Restriction map for
pF1KB3134
(Circular) (Six-base) MAPSORT of: pF1KB3134.seq Check: 6186 from: 1 to: 6803
pF1KB3134 6803 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1800 1800
Size: 6803
AccI GT'mk_AC
Cuts at: 3841 3841
Size: 6803
Acc65I G'GTAC_C
Cuts at: 132 3819 132
Size: 3687 3116
AclI AA'CG_TT
Cuts at: 6643 6643
Size: 6803
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 4628 5060 5413 6075 415
Size: 4213 432 353 662 1143
Fragments arranged by size:
4213 1143 662 432 353
AflIII A'CryG_T
Cuts at: 4155 5955 6236 4155
Size: 1800 281 4722
Fragments arranged by size:
4722 1800 281
AleI CACnn'nnGTG
Cuts at: 3517 3517
Size: 6803
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1474 1506 2816 2848 3118 3150 1474
Size: 32 1310 32 270 32 5127
Fragments arranged by size:
5127 1310 270 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 850 2644 3477 4108 5546 850
Size: 1794 833 631 1438 2107
Fragments arranged by size:
2107 1794 1438 833 631
ApaLI G'TGCA_C
Cuts at: 5641 5641
Size: 6803
ApoI r'AATT_y
Cuts at: 333 395 2456 2863 3807 6156 6412 333
Size: 62 2061 407 944 2349 256 724
Fragments arranged by size:
2349 2061 944 724 407 256 62
AseI AT'TA_AT
Cuts at: 20 2180 20
Size: 2160 4643
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6803
AvaI C'yCGr_G
Cuts at: 1651 3347 3823 1651
Size: 1696 476 4631
Fragments arranged by size:
4631 1696 476
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2694 2727 2694
Size: 33 6770
BamHI G'GATC_C
Cuts at: 1600 2306 3828 1600
Size: 706 1522 4575
Fragments arranged by size:
4575 1522 706
BanI G'GyrC_C
Cuts at: 132 1221 3819 4233 4481 4516 132
Size: 1089 2598 414 248 35 2419
Fragments arranged by size:
2598 2419 1089 414 248 35
BanII G_rGCy'C
Cuts at: 1391 1419 2000 3362 3817 4847 1391
Size: 28 581 1362 455 1030 3347
Fragments arranged by size:
3347 1362 1030 581 455 28
BbeI G_GCGC'C
Cuts at: 4237 4485 4237
Size: 248 6555
BbsI GAAGACnn'nnnn_
Cuts at: 911 1787 2073 2369 3434 911
Size: 876 286 296 1065 4280
Fragments arranged by size:
4280 1065 876 296 286
BbvCI CC'TCA_GC
Cuts at: 1451 1586 1768 2966 3368 1451
Size: 135 182 1198 402 4886
Fragments arranged by size:
4886 1198 402 182 135
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 6769
BciVI GTATCCnnnnn_n'
Cuts at: 4701 5757 6803 4701
Size: 1056 1046 4701
Fragments arranged by size:
4701 1056 1046
BclI T'GATC_A
Cuts at: 195 254 1592 4323 195
Size: 59 1338 2731 2675
Fragments arranged by size:
2731 2675 1338 59
BglII A'GATC_T
Cuts at: 3167 3222 3618 4318 6355 3167
Size: 55 396 700 2037 3615
Fragments arranged by size:
3615 2037 700 396 55
BlpI GC'TnA_GC
Cuts at: 503 3356 3915 503
Size: 2853 559 3391
Fragments arranged by size:
3391 2853 559
Bme1580I G_kGCm'C
Cuts at: 1940 4428 4521 5645 1940
Size: 2488 93 1124 3098
Fragments arranged by size:
3098 2488 1124 93
BmgBI CAC'GTC
Cuts at: 1710 1892 1710
Size: 182 6621
BmrI ACTGGGnnnn_n'
Cuts at: 297 2589 4015 4096 4431 6575 297
Size: 2292 1426 81 335 2144 525
Fragments arranged by size:
2292 2144 1426 525 335 81
BmtI G_CTAG'C
Cuts at: 3014 3022 3014
Size: 8 6795
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3167 5170 3167
Size: 2003 4800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2230 3466 3499 2230
Size: 1236 33 5534
Fragments arranged by size:
5534 1236 33
Bpu10I CC'TnA_GC
Cuts at: 491 1451 1586 1768 2966 3142 3368 3466 491
Size: 960 135 182 1198 176 226 98 3828
Fragments arranged by size:
3828 1198 960 226 182 176 135 98
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 556 1345 1555 2118 2125 3247 3979 4858
Size: 300 789 210 563 7 1122 732 879
Cuts at: 4858 5572 5870 256
Size: 714 298 1189
Fragments arranged by size:
1189 1122 879 789 732 714 563 300
298 210 7
BsaI GGTCTCn'nnnn_
Cuts at: 1513 6507 1513
Size: 4994 1809
BsaAI yAC'GTr
Cuts at: 4156 4156
Size: 6803
BsaBI GATnn'nnATC
Cuts at: 6 256 980 3418 4317 6354 6
Size: 250 724 2438 899 2037 455
Fragments arranged by size:
2438 2037 899 724 455 250
BsaHI Gr'CG_yC
Cuts at: 297 4234 4482 5184 6680 297
Size: 3937 248 702 1496 420
Fragments arranged by size:
3937 1496 702 420 248
BsaWI w'CCGG_w
Cuts at: 437 2516 3992 4513 5191 5602 5749 6023 437
Size: 2079 1476 521 678 411 147 274 1217
Fragments arranged by size:
2079 1476 1217 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1474 1504 2295 2325 2816 2846 3118 3148 3439
Size: 30 791 30 491 30 272 30 291
Cuts at: 3439 3469 3782 3812 1474
Size: 30 313 30 4465
Fragments arranged by size:
4465 791 491 313 291 272 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1423 1468 1615 1688 3673 1423
Size: 45 147 73 1985 4553
Fragments arranged by size:
4553 1985 147 73 45
BseYI C'CCAG_C
Cuts at: 1973 2427 4228 4986 5651 6059 1973
Size: 454 1801 758 665 408 2717
Fragments arranged by size:
2717 1801 758 665 454 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1141 1471 2605 2938 1141
Size: 330 1134 333 5006
Fragments arranged by size:
5006 1134 333 330
BsiEI CG_ry'CG
Cuts at: 104 4345 4391 5621 104
Size: 4241 46 1230 1286
Fragments arranged by size:
4241 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2000 2222 3362 3817 4595 4785 5645 2000
Size: 222 1140 455 778 190 860 3158
Fragments arranged by size:
3158 1140 860 778 455 222 190
BsmI GAATG_Cn'
Cuts at: 743 1745 743
Size: 1002 5801
Bsp1286I G_dGCh'C
Cuts at: 1391 1419 1940 2000 2222 3362 3817 4428 4521
Size: 28 521 60 222 1140 455 611 93
Cuts at: 4521 4595 4785 4847 5645 1391
Size: 74 190 62 798 2549
Fragments arranged by size:
2549 1140 798 611 521 455 222 190
93 74 62 60 28
BspEI T'CCGG_A
Cuts at: 2516 2516
Size: 6803
BspHI T'CATG_A
Cuts at: 1313 3773 5235 1313
Size: 2460 1462 2881
Fragments arranged by size:
2881 2460 1462
BspMI ACCTGCnnnn'nnnn_
Cuts at: 144 498 1493 1800 2918 3853 4067 4369 4750
Size: 354 995 307 1118 935 214 302 381
Cuts at: 4750 144
Size: 2197
Fragments arranged by size:
2197 1118 995 935 381 354 307 302
214
BsrBI CCG'CTC
Cuts at: 50 5095 5149 6633 6798 50
Size: 5045 54 1484 165 55
Fragments arranged by size:
5045 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3045 3362 4715 3045
Size: 317 1353 5133
Fragments arranged by size:
5133 1353 317
BsrFI r'CCGG_y
Cuts at: 1214 4801 4982 1214
Size: 3587 181 3035
Fragments arranged by size:
3587 3035 181
BsrGI T'GTAC_A
Cuts at: 1266 1266
Size: 6803
BssSI C'ACGA_G
Cuts at: 1954 5074 5782 1954
Size: 3120 708 2975
Fragments arranged by size:
3120 2975 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2119 2644 4180 2119
Size: 525 1536 4742
Fragments arranged by size:
4742 1536 525
BstBI TT'CG_AA
Cuts at: 2868 5164 2868
Size: 2296 4507
BstEII G'GTnAC_C
Cuts at: 229 229
Size: 6803
BstXI CCAn_nnnn'nTGG
Cuts at: 603 603
Size: 6803
BstYI r'GATC_y
Cuts at: 1 1018 1600 2091 2306 3043 3167 3222 3618
Size: 1017 582 491 215 737 124 55 396
Cuts at: 3618 3828 4295 4318 4653 4899 5205 5217 5303
Size: 210 467 23 335 246 306 12 86
Cuts at: 5303 6355 1
Size: 1052 449
Fragments arranged by size:
1052 1017 737 582 491 467 449 396
335 306 246 215 210 124 86 55
23 12
Bsu36I CC'TnA_GG
Cuts at: 1406 1406
Size: 6803
BtgI C'CryG_G
Cuts at: 1553 1613 2159 2600 2792 3605 1553
Size: 60 546 441 192 813 4751
Fragments arranged by size:
4751 813 546 441 192 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3766 4753 4934 5097 3766
Size: 987 181 163 5472
Fragments arranged by size:
5472 987 181 163
BtsI GCAGTG_nn'
Cuts at: 3301 4046 3301
Size: 745 6058
DraI TTT'AAA
Cuts at: 2325 3802 2325
Size: 1477 5326
DraIII CAC_nnn'GTG
Cuts at: 2628 2628
Size: 6803
DrdI GACnn_nn'nnGTC
Cuts at: 4509 5853 4509
Size: 1344 5459
EaeI y'GGCC_r
Cuts at: 605 1814 4388 4562 4953 4980 6325 605
Size: 1209 2574 174 391 27 1345 1083
Fragments arranged by size:
2574 1345 1209 1083 391 174 27
EagI C'GGCC_G
Cuts at: 4388 4388
Size: 6803
EarI CTCTTCn'nnn_
Cuts at: 2572 4826 5036 2572
Size: 2254 210 4339
Fragments arranged by size:
4339 2254 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 607 5011 5753 5899 6613 607
Size: 4404 742 146 714 797
Fragments arranged by size:
4404 797 742 714 146
EcoICRI GAG'CTC
Cuts at: 1998 3360 3815 1998
Size: 1362 455 4986
Fragments arranged by size:
4986 1362 455
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 2230 3466 3499 4628 5060 5413 6075 415
Size: 1815 1236 33 1129 432 353 662 1143
Fragments arranged by size:
1815 1236 1143 1129 662 432 353 33
EcoNI CCTnn'n_nnAGG
Cuts at: 1229 1281 1361 1229
Size: 52 80 6671
Fragments arranged by size:
6671 80 52
EcoO109I rG'GnC_Cy
Cuts at: 553 1118 1274 1903 2079 3942 553
Size: 565 156 629 176 1863 3414
Fragments arranged by size:
3414 1863 629 565 176 156
EcoRI G'AATT_C
Cuts at: 395 3807 395
Size: 3412 3391
EcoRV GAT'ATC
Cuts at: 243 2166 243
Size: 1923 4880
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 445 4822 445
Size: 4377 2426
FspI TGC'GCA
Cuts at: 4584 4584
Size: 6803
HaeII r_GCGC'y
Cuts at: 4237 4485 5715 6486 4237
Size: 248 1230 771 4554
Fragments arranged by size:
4554 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 339 371 3049 3081 4645 4675 4677 4707 339
Size: 32 2678 32 1564 30 2 30 2435
Fragments arranged by size:
2678 2435 1564 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2235 3388 3842 2235
Size: 1153 454 5196
Fragments arranged by size:
5196 1153 454
HindIII A'AGCT_T
Cuts at: 532 1801 532
Size: 1269 5534
KasI G'GCGC_C
Cuts at: 4233 4481 4233
Size: 248 6555
KpnI G_GTAC'C
Cuts at: 136 3823 136
Size: 3687 3116
MluI A'CGCG_T
Cuts at: 6236 6236
Size: 6803
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 107 287 2836 3575 5562 5746 107
Size: 180 2549 739 1987 184 1164
Fragments arranged by size:
2549 1987 1164 739 184 180
MscI TGG'CCA
Cuts at: 607 4564 607
Size: 3957 2846
MslI CAynn'nnrTG
Cuts at: 974 1712 3517 4792 4919 974
Size: 738 1805 1275 127 2858
Fragments arranged by size:
2858 1805 1275 738 127
MspA1I CmG'CkG
Cuts at: 325 1194 1495 1839 2110 3444 3474 3914 4228
Size: 869 301 344 271 1334 30 440 314
Cuts at: 4228 4588 5372 5617 325
Size: 360 784 245 1511
Fragments arranged by size:
1511 1334 869 784 440 360 344 314
301 271 245 30
NaeI GCC'GGC
Cuts at: 1216 4984 1216
Size: 3768 3035
NarI GG'CG_CC
Cuts at: 4234 4482 4234
Size: 248 6555
NcoI C'CATG_G
Cuts at: 2159 2600 2792 3605 2159
Size: 441 192 813 5357
Fragments arranged by size:
5357 813 441 192
NgoMIV G'CCGG_C
Cuts at: 1214 4982 1214
Size: 3768 3035
NheI G'CTAG_C
Cuts at: 3010 3018 3010
Size: 8 6795
NspI r_CATG'y
Cuts at: 2117 3856 4356 5959 2117
Size: 1739 500 1603 2961
Fragments arranged by size:
2961 1739 1603 500
PasI CC'CwG_GG
Cuts at: 1907 1907
Size: 6803
PciI A'CATG_T
Cuts at: 5955 5955
Size: 6803
PflMI CCAn_nnn'nTGG
Cuts at: 2160 2601 2606 2692 2160
Size: 441 5 86 6271
Fragments arranged by size:
6271 441 86 5
PfoI T'CCnGG_A
Cuts at: 671 1535 3422 671
Size: 864 1887 4052
Fragments arranged by size:
4052 1887 864
PmeI GTTT'AAAC
Cuts at: 3802 3802
Size: 6803
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1474 1506 2816 2848 3118 3150 5249 5281 1474
Size: 32 1310 32 270 32 2099 32 2996
Fragments arranged by size:
2996 2099 1310 270 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 553 1118 1903 553
Size: 565 785 5453
Fragments arranged by size:
5453 785 565
PshAI GACnn'nnGTC
Cuts at: 1862 3557 1862
Size: 1695 5108
PsiI TTA'TAA
Cuts at: 205 205
Size: 6803
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 360 392 360
Size: 32 6771
PstI C_TGCA'G
Cuts at: 687 1172 1811 2075 2174 2640 3850 4535 687
Size: 485 639 264 99 466 1210 685 2955
Fragments arranged by size:
2955 1210 685 639 485 466 264 99
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6803
PvuII CAG'CTG
Cuts at: 1194 1839 2110 3444 3474 4228 4588 1194
Size: 645 271 1334 30 754 360 3409
Fragments arranged by size:
3409 1334 754 645 360 271 30
RsrII CG'GwC_CG
Cuts at: 4998 4998
Size: 6803
SacI G_AGCT'C
Cuts at: 2000 3362 3817 2000
Size: 1362 455 4986
Fragments arranged by size:
4986 1362 455
SalI G'TCGA_C
Cuts at: 3840 3840
Size: 6803
SanDI GG'GwC_CC
Cuts at: 553 1903 553
Size: 1350 5453
SapI GCTCTTCn'nnn_
Cuts at: 4826 5036 4826
Size: 210 6593
SbfI CC_TGCA'GG
Cuts at: 3850 3850
Size: 6803
ScaI AGT'ACT
Cuts at: 1320 6320 1320
Size: 5000 1803
SfcI C'TryA_G
Cuts at: 33 683 871 1168 1189 1807 2071 2170 2636
Size: 650 188 297 21 618 264 99 466
Cuts at: 2636 3541 3846 4531 5499 5690 33
Size: 905 305 685 968 191 1146
Fragments arranged by size:
1146 968 905 685 650 618 466 305
297 264 191 188 99 21
SfoI GGC'GCC
Cuts at: 4235 4483 4235
Size: 248 6555
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6803
SmaI CCC'GGG
Cuts at: 3349 3825 3349
Size: 476 6327
SmlI C'TyrA_G
Cuts at: 235 535 1324 1570 1651 2097 2140 3226 3958
Size: 300 789 246 81 446 43 1086 732
Cuts at: 3958 4873 5587 5849 235
Size: 915 714 262 1189
Fragments arranged by size:
1189 1086 915 789 732 714 446 300
262 246 81 43
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6803
SphI G_CATG'C
Cuts at: 3856 4356 3856
Size: 500 6303
SspI AAT'ATT
Cuts at: 1340 2803 1340
Size: 1463 5340
StuI AGG'CCT
Cuts at: 3293 3293
Size: 6803
StyI C'CwwG_G
Cuts at: 911 951 2123 2159 2600 2792 2846 3605 3937
Size: 40 1172 36 441 192 54 759 332
Cuts at: 3937 4291 911
Size: 354 3423
Fragments arranged by size:
3423 1172 759 441 354 332 192 54
40 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 217 1841 2461 3598 4386 4519 4978 5187 217
Size: 1624 620 1137 788 133 459 209 1833
Fragments arranged by size:
1833 1624 1137 788 620 459 209 133
TatI w'GTAC_w
Cuts at: 417 1266 1318 2369 6318 417
Size: 849 52 1051 3949 902
Fragments arranged by size:
3949 1051 902 849 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1870 1902 2295 2327 6320 6352 1870
Size: 32 393 32 3993 32 2321
Fragments arranged by size:
3993 2321 393 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1828 4600 1828
Size: 2772 4031
XbaI T'CTAG_A
Cuts at: 3834 3834
Size: 6803
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1560 1858 4298 1560
Size: 298 2440 4065
Fragments arranged by size:
4065 2440 298
XhoI C'TCGA_G
Cuts at: 1651 1651
Size: 6803
XmaI C'CCGG_G
Cuts at: 3347 3823 3347
Size: 476 6327
XmnI GAAnn'nnTTC
Cuts at: 395 428 827 2831 3620 395
Size: 33 399 2004 789 3578
Fragments arranged by size:
3578 2004 789 399 33
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI ApaI AscI
AvrII BfrBI BglI BsiWI BsmBI BssHII BstZ17I ClaI
CspCI FseI FspAI HpaI MfeI NdeI NotI NruI
NsiI PacI PmlI PspOMI PspXI SacII SexAI SfiI
SgrAI SnaBI SrfI SwaI ZraI