Restriction map for
pF1KB3158
(Circular) (Six-base) MAPSORT of: pF1KB3158.seq Check: 4567 from: 1 to: 5885
pF1KB3158 5885 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 117 2336 2923 117
Size: 2219 587 3079
Fragments arranged by size:
3079 2219 587
Acc65I G'GTAC_C
Cuts at: 2901 2901
Size: 5885
AclI AA'CG_TT
Cuts at: 5725 5725
Size: 5885
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 177 366 437 579 1941 2394 3710 4142 4495
Size: 189 71 142 1362 453 1316 432 353
Cuts at: 4495 5157 177
Size: 662 905
Fragments arranged by size:
1362 1316 905 662 453 432 353 189
142 71
AflIII A'CryG_T
Cuts at: 3237 5037 5318 3237
Size: 1800 281 3804
Fragments arranged by size:
3804 1800 281
AgeI A'CCGG_T
Cuts at: 352 352
Size: 5885
AhdI GACnn_n'nnGTC
Cuts at: 330 330
Size: 5885
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1178 1210 1178
Size: 32 5853
AlwNI CAG_nnn'CTG
Cuts at: 2023 3190 4628 2023
Size: 1167 1438 3280
Fragments arranged by size:
3280 1438 1167
ApaLI G'TGCA_C
Cuts at: 4723 4723
Size: 5885
ApoI r'AATT_y
Cuts at: 1793 2799 2889 5238 5494 1793
Size: 1006 90 2349 256 2184
Fragments arranged by size:
2349 2184 1006 256 90
AseI AT'TA_AT
Cuts at: 20 749 965 2615 20
Size: 729 216 1650 3290
Fragments arranged by size:
3290 1650 729 216
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5885
AvaI C'yCGr_G
Cuts at: 202 395 2515 2905 202
Size: 193 2120 390 3182
Fragments arranged by size:
3182 2120 390 193
AvrII C'CTAG_G
Cuts at: 2395 2395
Size: 5885
BamHI G'GATC_C
Cuts at: 2910 2910
Size: 5885
BanI G'GyrC_C
Cuts at: 1033 2901 3315 3563 3598 1033
Size: 1868 414 248 35 3320
Fragments arranged by size:
3320 1868 414 248 35
BanII G_rGCy'C
Cuts at: 2477 2899 3929 2477
Size: 422 1030 4433
Fragments arranged by size:
4433 1030 422
BbeI G_GCGC'C
Cuts at: 3319 3567 3319
Size: 248 5637
BbvCI CC'TCA_GC
Cuts at: 902 2107 902
Size: 1205 4680
BciVI GTATCCnnnnn_n'
Cuts at: 3783 4839 5885 3783
Size: 1056 1046 3783
Fragments arranged by size:
3783 1056 1046
BclI T'GATC_A
Cuts at: 2502 3405 2502
Size: 903 4982
BglI GCCn_nnn'nGGC
Cuts at: 1900 1900
Size: 5885
BglII A'GATC_T
Cuts at: 665 3400 5437 665
Size: 2735 2037 1113
Fragments arranged by size:
2735 2037 1113
BlpI GC'TnA_GC
Cuts at: 2997 2997
Size: 5885
Bme1580I G_kGCm'C
Cuts at: 1036 3510 3603 4727 1036
Size: 2474 93 1124 2194
Fragments arranged by size:
2474 2194 1124 93
BmgBI CAC'GTC
Cuts at: 804 804
Size: 5885
BmrI ACTGGGnnnn_n'
Cuts at: 3097 3178 3513 5657 3097
Size: 81 335 2144 3325
Fragments arranged by size:
3325 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1686 4252 1686
Size: 2566 3319
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1178 1219 1178
Size: 41 5844
Bpu10I CC'TnA_GC
Cuts at: 458 902 2107 458
Size: 444 1205 4236
Fragments arranged by size:
4236 1205 444
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3061 3940 4654 4952 3061
Size: 879 714 298 3994
Fragments arranged by size:
3994 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 920 1489 5589 920
Size: 569 4100 1216
Fragments arranged by size:
4100 1216 569
BsaAI yAC'GTr
Cuts at: 417 3238 417
Size: 2821 3064
BsaBI GATnn'nnATC
Cuts at: 6 619 3399 5436 6
Size: 613 2780 2037 455
Fragments arranged by size:
2780 2037 613 455
BsaHI Gr'CG_yC
Cuts at: 114 3316 3564 4266 5762 114
Size: 3202 248 702 1496 237
Fragments arranged by size:
3202 1496 702 248 237
BsaWI w'CCGG_w
Cuts at: 352 3074 3595 4273 4684 4831 5105 352
Size: 2722 521 678 411 147 274 1132
Fragments arranged by size:
2722 1132 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 466 496 1178 1208 2376 2406 466
Size: 30 682 30 1168 30 3945
Fragments arranged by size:
3945 1168 682 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 522 525 823 1735 2184 2234 2638 522
Size: 3 298 912 449 50 404 3769
Fragments arranged by size:
3769 912 449 404 298 50 3
BseYI C'CCAG_C
Cuts at: 3310 4068 4733 5141 3310
Size: 758 665 408 4054
Fragments arranged by size:
4054 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 430
Size: 5885
BsiEI CG_ry'CG
Cuts at: 104 3427 3473 4703 104
Size: 3323 46 1230 1286
Fragments arranged by size:
3323 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 269 2899 3677 3867 4727 269
Size: 2630 778 190 860 1427
Fragments arranged by size:
2630 1427 860 778 190
BsmI GAATG_Cn'
Cuts at: 1026 2361 1026
Size: 1335 4550
Bsp1286I G_dGCh'C
Cuts at: 269 1036 2477 2899 3510 3603 3677 3867 3929
Size: 767 1441 422 611 93 74 190 62
Cuts at: 3929 4727 269
Size: 798 1427
Fragments arranged by size:
1441 1427 798 767 611 422 190 93
74 62
BspHI T'CATG_A
Cuts at: 4317 4317
Size: 5885
BspMI ACCTGCnnnn'nnnn_
Cuts at: 161 299 2935 3149 3451 3832 161
Size: 138 2636 214 302 381 2214
Fragments arranged by size:
2636 2214 381 302 214 138
BsrBI CCG'CTC
Cuts at: 50 434 515 4177 4231 5715 5880 50
Size: 384 81 3662 54 1484 165 55
Fragments arranged by size:
3662 1484 384 165 81 55 54
BsrDI GCAATG_nn'
Cuts at: 1376 2452 3797 1376
Size: 1076 1345 3464
Fragments arranged by size:
3464 1345 1076
BsrFI r'CCGG_y
Cuts at: 352 3883 4064 352
Size: 3531 181 2173
Fragments arranged by size:
3531 2173 181
BsrGI T'GTAC_A
Cuts at: 233 233
Size: 5885
BssSI C'ACGA_G
Cuts at: 4156 4864 4156
Size: 708 5177
BstAPI GCAn_nnn'nTGC
Cuts at: 3262 3262
Size: 5885
BstBI TT'CG_AA
Cuts at: 4246 4246
Size: 5885
BstXI CCAn_nnnn'nTGG
Cuts at: 2538 2733 2538
Size: 195 5690
BstYI r'GATC_y
Cuts at: 1 665 1398 2910 3377 3400 3735 3981 4287
Size: 664 733 1512 467 23 335 246 306
Cuts at: 4287 4299 4385 5437 1
Size: 12 86 1052 449
Fragments arranged by size:
1512 1052 733 664 467 449 335 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 584 584
Size: 5885
BtgI C'CryG_G
Cuts at: 107 257 783 107
Size: 150 526 5209
Fragments arranged by size:
5209 526 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2464 3835 4016 4179 2464
Size: 1371 181 163 4170
Fragments arranged by size:
4170 1371 181 163
BtsI GCAGTG_nn'
Cuts at: 939 1256 3128 939
Size: 317 1872 3696
Fragments arranged by size:
3696 1872 317
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 908 943 908
Size: 35 5850
DraI TTT'AAA
Cuts at: 2884 2884
Size: 5885
DrdI GACnn_nn'nnGTC
Cuts at: 3591 4935 3591
Size: 1344 4541
EaeI y'GGCC_r
Cuts at: 281 408 710 3470 3644 4035 4062 5407 281
Size: 127 302 2760 174 391 27 1345 759
Fragments arranged by size:
2760 1345 759 391 302 174 127 27
EagI C'GGCC_G
Cuts at: 3470 3470
Size: 5885
EarI CTCTTCn'nnn_
Cuts at: 184 988 1655 1820 2382 2441 3908 4118 184
Size: 804 667 165 562 59 1467 210 1951
Fragments arranged by size:
1951 1467 804 667 562 210 165 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4093 4835 4981 5695 4093
Size: 742 146 714 4283
Fragments arranged by size:
4283 742 714 146
EcoICRI GAG'CTC
Cuts at: 2897 2897
Size: 5885
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 177 366 437 579 1178 1219 1941 2394 3710
Size: 189 71 142 599 41 722 453 1316
Cuts at: 3710 4142 4495 5157 177
Size: 432 353 662 905
Fragments arranged by size:
1316 905 722 662 599 453 432 353
189 142 71 41
EcoNI CCTnn'n_nnAGG
Cuts at: 244 283 583 244
Size: 39 300 5546
Fragments arranged by size:
5546 300 39
EcoO109I rG'GnC_Cy
Cuts at: 187 288 477 678 1750 2392 3024 187
Size: 101 189 201 1072 642 632 3048
Fragments arranged by size:
3048 1072 642 632 201 189 101
EcoRI G'AATT_C
Cuts at: 1793 2889 1793
Size: 1096 4789
EcoRV GAT'ATC
Cuts at: 2229 2229
Size: 5885
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1615 3904 1615
Size: 2289 3596
FspI TGC'GCA
Cuts at: 3666 3666
Size: 5885
HaeII r_GCGC'y
Cuts at: 3319 3567 4797 5568 3319
Size: 248 1230 771 3636
Fragments arranged by size:
3636 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 316 348 418 450 898 930 1068 1100 1559
Size: 32 70 32 448 32 138 32 459
Cuts at: 1559 1591 1747 1779 1835 1867 2376 2408 3727
Size: 32 156 32 56 32 509 32 1319
Cuts at: 3727 3757 3759 3789 316
Size: 30 2 30 2412
Fragments arranged by size:
2412 1319 509 459 448 156 138 70
56 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1368 1422 1863 2337 2924 1368
Size: 54 441 474 587 4329
Fragments arranged by size:
4329 587 474 441 54
HindIII A'AGCT_T
Cuts at: 2715 2715
Size: 5885
HpaI GTT'AAC
Cuts at: 1368 1422 1368
Size: 54 5831
KasI G'GCGC_C
Cuts at: 3315 3563 3315
Size: 248 5637
KpnI G_GTAC'C
Cuts at: 2905 2905
Size: 5885
MfeI C'AATT_G
Cuts at: 972 1040 972
Size: 68 5817
MluI A'CGCG_T
Cuts at: 5318 5318
Size: 5885
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1895 4644 4828 1895
Size: 2749 184 2952
Fragments arranged by size:
2952 2749 184
MscI TGG'CCA
Cuts at: 410 712 3646 410
Size: 302 2934 2649
Fragments arranged by size:
2934 2649 302
MslI CAynn'nnrTG
Cuts at: 1145 1591 2350 2536 2743 3874 4001 1145
Size: 446 759 186 207 1131 127 3029
Fragments arranged by size:
3029 1131 759 446 207 186 127
MspA1I CmG'CkG
Cuts at: 197 259 339 1048 1815 2996 3310 3670 4454
Size: 62 80 709 767 1181 314 360 784
Cuts at: 4454 4699 197
Size: 245 1383
Fragments arranged by size:
1383 1181 784 767 709 360 314 245
80 62
NaeI GCC'GGC
Cuts at: 4066 4066
Size: 5885
NarI GG'CG_CC
Cuts at: 3316 3564 3316
Size: 248 5637
NcoI C'CATG_G
Cuts at: 107 783 107
Size: 676 5209
NdeI CA'TA_TG
Cuts at: 2843 2843
Size: 5885
NgoMIV G'CCGG_C
Cuts at: 4064 4064
Size: 5885
NspI r_CATG'y
Cuts at: 2938 3438 5041 2938
Size: 500 1603 3782
Fragments arranged by size:
3782 1603 500
PasI CC'CwG_GG
Cuts at: 2786 2786
Size: 5885
PciI A'CATG_T
Cuts at: 5037 5037
Size: 5885
PflMI CCAn_nnn'nTGG
Cuts at: 1059 1990 1059
Size: 931 4954
PfoI T'CCnGG_A
Cuts at: 680 680
Size: 5885
PmeI GTTT'AAAC
Cuts at: 2884 2884
Size: 5885
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1178 1210 4331 4363 1178
Size: 32 3121 32 2700
Fragments arranged by size:
3121 2700 32 32
PpuMI rG'GwC_Cy
Cuts at: 187 288 678 1750 2392 187
Size: 101 390 1072 642 3680
Fragments arranged by size:
3680 1072 642 390 101
PshAI GACnn'nnGTC
Cuts at: 551 1931 551
Size: 1380 4505
PstI C_TGCA'G
Cuts at: 315 2462 2932 3617 315
Size: 2147 470 685 2583
Fragments arranged by size:
2583 2147 685 470
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5885
PvuII CAG'CTG
Cuts at: 197 339 1048 1815 3310 3670 197
Size: 142 709 767 1495 360 2412
Fragments arranged by size:
2412 1495 767 709 360 142
RsrII CG'GwC_CG
Cuts at: 4080 4080
Size: 5885
SacI G_AGCT'C
Cuts at: 2899 2899
Size: 5885
SacII CC_GC'GG
Cuts at: 260 260
Size: 5885
SalI G'TCGA_C
Cuts at: 2335 2922 2335
Size: 587 5298
SanDI GG'GwC_CC
Cuts at: 187 678 187
Size: 491 5394
SapI GCTCTTCn'nnn_
Cuts at: 3908 4118 3908
Size: 210 5675
SbfI CC_TGCA'GG
Cuts at: 2932 2932
Size: 5885
ScaI AGT'ACT
Cuts at: 2288 5402 2288
Size: 3114 2771
SfcI C'TryA_G
Cuts at: 33 144 311 1025 1522 1703 2198 2458 2928
Size: 111 167 714 497 181 495 260 470
Cuts at: 2928 3613 4581 4772 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 968 714 685 497 495 470 260
191 181 167 111
SfoI GGC'GCC
Cuts at: 3317 3565 3317
Size: 248 5637
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5885
SgrAI Cr'CCGG_yG
Cuts at: 352 352
Size: 5885
SmaI CCC'GGG
Cuts at: 397 2907 397
Size: 2510 3375
SmlI C'TyrA_G
Cuts at: 3040 3955 4669 4931 3040
Size: 915 714 262 3994
Fragments arranged by size:
3994 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5885
SphI G_CATG'C
Cuts at: 2938 3438 2938
Size: 500 5385
SspI AAT'ATT
Cuts at: 1486 2877 1486
Size: 1391 4494
StuI AGG'CCT
Cuts at: 2400 2400
Size: 5885
StyI C'CwwG_G
Cuts at: 107 783 2395 3019 3373 107
Size: 676 1612 624 354 2619
Fragments arranged by size:
2619 1612 676 624 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 841 1689 1835 1893 3468 3601 4060 4269 841
Size: 848 146 58 1575 133 459 209 2457
Fragments arranged by size:
2457 1575 848 459 209 146 133 58
TatI w'GTAC_w
Cuts at: 233 1838 2286 2808 5400 233
Size: 1605 448 522 2592 718
Fragments arranged by size:
2592 1605 718 522 448
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 466 498 5402 5434 466
Size: 32 4904 32 917
Fragments arranged by size:
4904 917 32 32
Tth111I GACn'n_nGTC
Cuts at: 1783 2563 3682 1783
Size: 780 1119 3986
Fragments arranged by size:
3986 1119 780
XbaI T'CTAG_A
Cuts at: 2223 2916 2223
Size: 693 5192
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1506 3380 1506
Size: 1874 4011
XmaI C'CCGG_G
Cuts at: 395 2905 395
Size: 2510 3375
XmnI GAAnn'nnTTC
Cuts at: 1979 1979
Size: 5885
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI ApaI AscI
BaeI BbsI BcgI BfrBI BmtI BsiWI BsmBI BspEI
BssHII BstEII BstZ17I ClaI DraIII FseI FspAI NheI
NotI NruI NsiI PacI PmlI PsiI PspOMI PspXI
PsrI SexAI SfiI SnaBI SrfI SwaI XhoI ZraI