Restriction map for
pF1KB3168
(Circular) (Six-base) MAPSORT of: pF1KB3168.seq Check: 4277 from: 1 to: 5483
pF1KB3168 5483 bp
With 182 enzymes: SgfI *
February 15, 2010 17:49 ..
AccI GT'mk_AC
Cuts at: 2521 2521
Size: 5483
Acc65I G'GTAC_C
Cuts at: 2499 2499
Size: 5483
AclI AA'CG_TT
Cuts at: 1596 5323 1596
Size: 3727 1756
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 649 916 3308 3740 4487 4755 649
Size: 267 2392 432 747 268 1377
Fragments arranged by size:
2392 1377 747 432 268 267
AflIII A'CryG_T
Cuts at: 440 2835 3939 4916 440
Size: 2395 1104 977 1007
Fragments arranged by size:
2395 1104 1007 977
AgeI A'CCGG_T
Cuts at: 3871 3871
Size: 5483
AleI CACnn'nnGTG
Cuts at: 2354 2354
Size: 5483
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2233 2233
Size: 5483
AlwNI CAG_nnn'CTG
Cuts at: 988 2788 4355 988
Size: 1800 1567 2116
Fragments arranged by size:
2116 1800 1567
ApaI G_GGCC'C
Cuts at: 1387 2341 2349 2400 1387
Size: 954 8 51 4470
Fragments arranged by size:
4470 954 51 8
ApaLI G'TGCA_C
Cuts at: 4253 4253
Size: 5483
ApoI r'AATT_y
Cuts at: 523 559 800 2487 4836 5092 523
Size: 36 241 1687 2349 256 914
Fragments arranged by size:
2349 1687 914 256 241 36
AseI AT'TA_AT
Cuts at: 20 20
Size: 5483
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5483
AvaI C'yCGr_G
Cuts at: 1411 1421 1660 2258 2503 1411
Size: 10 239 598 245 4391
Fragments arranged by size:
4391 598 245 239 10
BamHI G'GATC_C
Cuts at: 1656 2508 1656
Size: 852 4631
BanI G'GyrC_C
Cuts at: 2095 2499 2913 3161 3196 2095
Size: 404 414 248 35 4382
Fragments arranged by size:
4382 414 404 248 35
BanII G_rGCy'C
Cuts at: 1387 1397 1442 1549 1828 2303 2341 2349 2400
Size: 10 45 107 279 475 38 8 51
Cuts at: 2400 2497 3527 1387
Size: 97 1030 3343
Fragments arranged by size:
3343 1030 475 279 107 97 51 45
38 10 8
BbeI G_GCGC'C
Cuts at: 2917 3165 2917
Size: 248 5235
BbsI GAAGACnn'nnnn_
Cuts at: 194 965 2378 194
Size: 771 1413 3299
Fragments arranged by size:
3299 1413 771
BbvCI CC'TCA_GC
Cuts at: 1759 1759
Size: 5483
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1392 1426 1849 1883 1392
Size: 34 423 34 4992
Fragments arranged by size:
4992 423 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2116 3381 4142 5483 2116
Size: 1265 761 1341 2116
Fragments arranged by size:
2116 1341 1265 761
BclI T'GATC_A
Cuts at: 2409 3003 2409
Size: 594 4889
BfrBI ATG'CAT
Cuts at: 423 423
Size: 5483
BglII A'GATC_T
Cuts at: 2998 5035 2998
Size: 2037 3446
BlpI GC'TnA_GC
Cuts at: 2595 2595
Size: 5483
Bme1580I G_kGCm'C
Cuts at: 1211 1241 1387 2341 2349 2400 3108 3201 4257
Size: 30 146 954 8 51 708 93 1056
Cuts at: 4257 1211
Size: 2437
Fragments arranged by size:
2437 1056 954 708 146 93 51 30
8
BmrI ACTGGGnnnn_n'
Cuts at: 1478 2695 2776 3111 5255 1478
Size: 1217 81 335 2144 1706
Fragments arranged by size:
2144 1706 1217 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1767 3850 1767
Size: 2083 3400
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 559 1666 1737 1943 167
Size: 392 1107 71 206 3707
Fragments arranged by size:
3707 1107 392 206 71
Bpu10I CC'TnA_GC
Cuts at: 1759 1759
Size: 5483
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 1868 2226 2659 3538 4030 4328 481
Size: 1387 358 433 879 492 298 1636
Fragments arranged by size:
1636 1387 879 492 433 358 298
BsaI GGTCTCn'nnnn_
Cuts at: 935 1516 5187 935
Size: 581 3671 1231
Fragments arranged by size:
3671 1231 581
BsaAI yAC'GTr
Cuts at: 2836 2836
Size: 5483
BsaBI GATnn'nnATC
Cuts at: 6 1039 2997 5034 6
Size: 1033 1958 2037 455
Fragments arranged by size:
2037 1958 1033 455
BsaHI Gr'CG_yC
Cuts at: 1862 2914 3162 3864 5360 1862
Size: 1052 248 702 1496 1985
Fragments arranged by size:
1985 1496 1052 702 248
BsaWI w'CCGG_w
Cuts at: 374 1820 2672 3193 3871 4145 4292 4703 374
Size: 1446 852 521 678 274 147 411 1154
Fragments arranged by size:
1446 1154 852 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1212 1474 1740 2311 1212
Size: 262 266 571 4384
Fragments arranged by size:
4384 571 266 262
BseYI C'CCAG_C
Cuts at: 1055 1241 1307 1501 2908 3666 4243 4739 1055
Size: 186 66 194 1407 758 577 496 1799
Fragments arranged by size:
1799 1407 758 577 496 194 186 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1560 1594 1874 1947 1560
Size: 34 280 73 5096
Fragments arranged by size:
5096 280 73 34
BsiEI CG_ry'CG
Cuts at: 104 3025 3071 4279 104
Size: 2921 46 1208 1308
Fragments arranged by size:
2921 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1330 2303 2497 3275 3465 4257 1330
Size: 973 194 778 190 792 2556
Fragments arranged by size:
2556 973 792 778 194 190
BsmI GAATG_Cn'
Cuts at: 838 1836 2095 2455 838
Size: 998 259 360 3866
Fragments arranged by size:
3866 998 360 259
Bsp1286I G_dGCh'C
Cuts at: 1211 1241 1330 1387 1397 1442 1549 1828 2303
Size: 30 89 57 10 45 107 279 475
Cuts at: 2303 2341 2349 2400 2497 3108 3201 3275 3465
Size: 38 8 51 97 611 93 74 190
Cuts at: 3465 3527 4257 1211
Size: 62 730 2437
Fragments arranged by size:
2437 730 611 475 279 190 107 97
93 89 74 62 57 51 45 38
30 10 8
BspEI T'CCGG_A
Cuts at: 374 1820 4703 374
Size: 1446 2883 1154
Fragments arranged by size:
2883 1446 1154
BspHI T'CATG_A
Cuts at: 2166 4659 2166
Size: 2493 2990
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2533 2747 3049 3430 2533
Size: 214 302 381 4586
Fragments arranged by size:
4586 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3775 3829 5313 5478 50
Size: 3725 54 1484 165 55
Fragments arranged by size:
3725 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2427 3395 2427
Size: 968 4515
BsrFI r'CCGG_y
Cuts at: 1936 3481 3662 3871 1936
Size: 1545 181 209 3548
Fragments arranged by size:
3548 1545 209 181
BssSI C'ACGA_G
Cuts at: 3754 4112 3754
Size: 358 5125
BstAPI GCAn_nnn'nTGC
Cuts at: 1721 2450 2860 1721
Size: 729 410 4344
Fragments arranged by size:
4344 729 410
BstBI TT'CG_AA
Cuts at: 3844 3844
Size: 5483
BstEII G'GTnAC_C
Cuts at: 888 888
Size: 5483
BstXI CCAn_nnnn'nTGG
Cuts at: 1852 1852
Size: 5483
BstYI r'GATC_y
Cuts at: 1 984 1656 1709 1803 2508 2975 2998 3333
Size: 983 672 53 94 705 467 23 335
Cuts at: 3333 3579 4591 4677 4689 5035 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 983 705 672 467 449 346 335
246 94 86 53 23 12
Bsu36I CC'TnA_GG
Cuts at: 128 1388 1457 2028 128
Size: 1260 69 571 3583
Fragments arranged by size:
3583 1260 571 69
BtgI C'CryG_G
Cuts at: 107 647 1062 1223 107
Size: 540 415 161 4367
Fragments arranged by size:
4367 540 415 161
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3433 3614 3777 3433
Size: 181 163 5139
Fragments arranged by size:
5139 181 163
BtsI GCAGTG_nn'
Cuts at: 1526 2726 1526
Size: 1200 4283
DraI TTT'AAA
Cuts at: 198 228 522 2482 198
Size: 30 294 1960 3199
Fragments arranged by size:
3199 1960 294 30
DraIII CAC_nnn'GTG
Cuts at: 1985 1985
Size: 5483
DrdI GACnn_nn'nnGTC
Cuts at: 3189 4047 3189
Size: 858 4625
EaeI y'GGCC_r
Cuts at: 3068 3242 3633 3660 5005 3068
Size: 174 391 27 1345 3546
Fragments arranged by size:
3546 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3068 3068
Size: 5483
EarI CTCTTCn'nnn_
Cuts at: 2180 3506 3716 2180
Size: 1326 210 3947
Fragments arranged by size:
3947 1326 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3691 4001 4147 5293 3691
Size: 310 146 1146 3881
Fragments arranged by size:
3881 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2301 2495 2301
Size: 194 5289
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 559 649 916 1666 1737 1943 3308 3740
Size: 392 90 267 750 71 206 1365 432
Cuts at: 3740 4487 4755 167
Size: 747 268 895
Fragments arranged by size:
1365 895 750 747 432 392 268 267
206 90 71
EcoNI CCTnn'n_nnAGG
Cuts at: 127 127
Size: 5483
EcoO109I rG'GnC_Cy
Cuts at: 1384 1415 1474 2338 2345 2397 2622 1384
Size: 31 59 864 7 52 225 4245
Fragments arranged by size:
4245 864 225 59 52 31 7
EcoRI G'AATT_C
Cuts at: 2487 2487
Size: 5483
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3502 3502
Size: 5483
FspI TGC'GCA
Cuts at: 3264 3264
Size: 5483
HaeII r_GCGC'y
Cuts at: 391 484 1411 2917 3165 4187 5166 391
Size: 93 927 1506 248 1022 979 708
Fragments arranged by size:
1506 1022 979 927 708 248 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 204 236 3325 3355 3357 3387 204
Size: 32 3089 30 2 30 2300
Fragments arranged by size:
3089 2300 32 30 30 2
HincII GTy'rAC
Cuts at: 936 2522 936
Size: 1586 3897
HindIII A'AGCT_T
Cuts at: 379 379
Size: 5483
HpaI GTT'AAC
Cuts at: 936 936
Size: 5483
KasI G'GCGC_C
Cuts at: 2913 3161 2913
Size: 248 5235
KpnI G_GTAC'C
Cuts at: 2503 2503
Size: 5483
MfeI C'AATT_G
Cuts at: 411 411
Size: 5483
MluI A'CGCG_T
Cuts at: 4916 4916
Size: 5483
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1076 4154 4338 1076
Size: 3078 184 2221
Fragments arranged by size:
3078 2221 184
MscI TGG'CCA
Cuts at: 3244 3244
Size: 5483
MslI CAynn'nnrTG
Cuts at: 646 1297 1531 2354 3472 3599 646
Size: 651 234 823 1118 127 2530
Fragments arranged by size:
2530 1118 823 651 234 127
MspA1I CmG'CkG
Cuts at: 530 2594 2908 3268 4281 4526 530
Size: 2064 314 360 1013 245 1487
Fragments arranged by size:
2064 1487 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1938 3664 1938
Size: 1726 3757
NarI GG'CG_CC
Cuts at: 2914 3162 2914
Size: 248 5235
NcoI C'CATG_G
Cuts at: 107 647 1062 1223 107
Size: 540 415 161 4367
Fragments arranged by size:
4367 540 415 161
NgoMIV G'CCGG_C
Cuts at: 1936 3662 1936
Size: 1726 3757
NsiI A_TGCA'T
Cuts at: 425 425
Size: 5483
NspI r_CATG'y
Cuts at: 427 444 2536 3036 3943 427
Size: 17 2092 500 907 1967
Fragments arranged by size:
2092 1967 907 500 17
PasI CC'CwG_GG
Cuts at: 2341 2341
Size: 5483
PciI A'CATG_T
Cuts at: 440 3939 440
Size: 3499 1984
PflMI CCAn_nnn'nTGG
Cuts at: 2334 2334
Size: 5483
PfoI T'CCnGG_A
Cuts at: 1682 1682
Size: 5483
PmeI GTTT'AAAC
Cuts at: 2482 2482
Size: 5483
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4622 4654 4622
Size: 32 5451
PspOMI G'GGCC_C
Cuts at: 1383 2337 2345 2396 1383
Size: 954 8 51 4470
Fragments arranged by size:
4470 954 51 8
PstI C_TGCA'G
Cuts at: 1717 2530 3215 1717
Size: 813 685 3985
Fragments arranged by size:
3985 813 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5483
PvuII CAG'CTG
Cuts at: 2908 3268 2908
Size: 360 5123
RsrII CG'GwC_CG
Cuts at: 3678 3678
Size: 5483
SacI G_AGCT'C
Cuts at: 2303 2497 2303
Size: 194 5289
SalI G'TCGA_C
Cuts at: 2520 2520
Size: 5483
SapI GCTCTTCn'nnn_
Cuts at: 2180 3506 3716 2180
Size: 1326 210 3947
Fragments arranged by size:
3947 1326 210
SbfI CC_TGCA'GG
Cuts at: 2530 2530
Size: 5483
ScaI AGT'ACT
Cuts at: 1024 1719 5000 1024
Size: 695 3281 1507
Fragments arranged by size:
3281 1507 695
SfcI C'TryA_G
Cuts at: 33 837 1713 1736 2526 3211 4204 4395 33
Size: 804 876 23 790 685 993 191 1121
Fragments arranged by size:
1121 993 876 804 790 685 191 23
SfoI GGC'GCC
Cuts at: 2915 3163 2915
Size: 248 5235
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5483
SmaI CCC'GGG
Cuts at: 1662 2260 2505 1662
Size: 598 245 4640
Fragments arranged by size:
4640 598 245
SmlI C'TyrA_G
Cuts at: 496 1883 2241 2638 3553 4045 4307 496
Size: 1387 358 397 915 492 262 1672
Fragments arranged by size:
1672 1387 915 492 397 358 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5483
SphI G_CATG'C
Cuts at: 427 2536 3036 427
Size: 2109 500 2874
Fragments arranged by size:
2874 2109 500
SrfI GCCC'GGGC
Cuts at: 2260 2260
Size: 5483
StuI AGG'CCT
Cuts at: 1747 1747
Size: 5483
StyI C'CwwG_G
Cuts at: 107 647 884 1050 1062 1223 1442 2100 2617
Size: 540 237 166 12 161 219 658 517
Cuts at: 2617 2971 107
Size: 354 2619
Fragments arranged by size:
2619 658 540 517 354 237 219 166
161 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 660 784 1321 1515 3066 3199 3658 3867 660
Size: 124 537 194 1551 133 459 209 2276
Fragments arranged by size:
2276 1551 537 459 209 194 133 124
TatI w'GTAC_w
Cuts at: 437 668 1022 1163 1717 1997 4998 437
Size: 231 354 141 554 280 3001 922
Fragments arranged by size:
3001 922 554 354 280 231 141
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1777 1809 5000 5032 1777
Size: 32 3191 32 2228
Fragments arranged by size:
3191 2228 32 32
Tth111I GACn'n_nGTC
Cuts at: 3280 3280
Size: 5483
XbaI T'CTAG_A
Cuts at: 2514 2514
Size: 5483
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2076 2331 2978 2076
Size: 255 647 4581
Fragments arranged by size:
4581 647 255
XmaI C'CCGG_G
Cuts at: 1660 2258 2503 1660
Size: 598 245 4640
Fragments arranged by size:
4640 598 245
XmnI GAAnn'nnTTC
Cuts at: 1151 2137 2453 1151
Size: 986 316 4181
Fragments arranged by size:
4181 986 316
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BglI BmgBI BmtI BsaXI BsiWI BsmBI BsrGI
BssHII BstZ17I ClaI CspCI EcoRV FseI FspAI NdeI
NheI NotI NruI PacI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SspI SwaI XhoI ZraI