Restriction map for
pF1KB3201
(Circular) (Six-base) MAPSORT of: pF1KB3201.seq Check: 822 from: 1 to: 4151
pF1KB3201 4151 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 1189 1189
Size: 4151
Acc65I G'GTAC_C
Cuts at: 1167 1167
Size: 4151
AclI AA'CG_TT
Cuts at: 3991 3991
Size: 4151
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 873 1976 2408 3155 3423 873
Size: 1103 432 747 268 1601
Fragments arranged by size:
1601 1103 747 432 268
AflII C'TTAA_G
Cuts at: 285 285
Size: 4151
AflIII A'CryG_T
Cuts at: 458 692 824 1503 2607 3584 458
Size: 234 132 679 1104 977 1025
Fragments arranged by size:
1104 1025 977 679 234 132
AgeI A'CCGG_T
Cuts at: 2539 2539
Size: 4151
AhdI GACnn_n'nnGTC
Cuts at: 123 123
Size: 4151
AlwNI CAG_nnn'CTG
Cuts at: 975 1456 3023 975
Size: 481 1567 2103
Fragments arranged by size:
2103 1567 481
ApaI G_GGCC'C
Cuts at: 648 648
Size: 4151
ApaLI G'TGCA_C
Cuts at: 2921 2921
Size: 4151
ApoI r'AATT_y
Cuts at: 1155 3504 3760 1155
Size: 2349 256 1546
Fragments arranged by size:
2349 1546 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4151
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
AvaI C'yCGr_G
Cuts at: 128 649 1171 128
Size: 521 522 3108
Fragments arranged by size:
3108 522 521
BamHI G'GATC_C
Cuts at: 503 1176 503
Size: 673 3478
BanI G'GyrC_C
Cuts at: 1167 1581 1829 1864 1167
Size: 414 248 35 3454
Fragments arranged by size:
3454 414 248 35
BanII G_rGCy'C
Cuts at: 601 648 1165 2195 601
Size: 47 517 1030 2557
Fragments arranged by size:
2557 1030 517 47
BbeI G_GCGC'C
Cuts at: 1585 1833 1585
Size: 248 3903
BbsI GAAGACnn'nnnn_
Cuts at: 826 826
Size: 4151
BciVI GTATCCnnnnn_n'
Cuts at: 2049 2810 4151 2049
Size: 761 1341 2049
Fragments arranged by size:
2049 1341 761
BclI T'GATC_A
Cuts at: 765 1671 765
Size: 906 3245
BglI GCCn_nnn'nGGC
Cuts at: 970 970
Size: 4151
BglII A'GATC_T
Cuts at: 915 1666 3703 915
Size: 751 2037 1363
Fragments arranged by size:
2037 1363 751
BlpI GC'TnA_GC
Cuts at: 1263 1263
Size: 4151
Bme1580I G_kGCm'C
Cuts at: 648 799 1776 1869 2925 648
Size: 151 977 93 1056 1874
Fragments arranged by size:
1874 1056 977 151 93
BmgBI CAC'GTC
Cuts at: 120 120
Size: 4151
BmrI ACTGGGnnnn_n'
Cuts at: 1363 1444 1779 3923 1363
Size: 81 335 2144 1591
Fragments arranged by size:
2144 1591 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2518 2518
Size: 4151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1327 2206 2698 2996 1327
Size: 879 492 298 2482
Fragments arranged by size:
2482 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 340 3855 340
Size: 3515 636
BsaAI yAC'GTr
Cuts at: 1504 1504
Size: 4151
BsaBI GATnn'nnATC
Cuts at: 6 1665 3702 6
Size: 1659 2037 455
Fragments arranged by size:
2037 1659 455
BsaHI Gr'CG_yC
Cuts at: 1582 1830 2532 4028 1582
Size: 248 702 1496 1705
Fragments arranged by size:
1705 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1340 1861 2539 2813 2960 3371 1340
Size: 521 678 274 147 411 2120
Fragments arranged by size:
2120 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 157 187 157
Size: 30 4121
BseYI C'CCAG_C
Cuts at: 1576 2334 2911 3407 1576
Size: 758 577 496 2320
Fragments arranged by size:
2320 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 914 914
Size: 4151
BsiEI CG_ry'CG
Cuts at: 104 1693 1739 2947 104
Size: 1589 46 1208 1308
Fragments arranged by size:
1589 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 954 1165 1943 2133 2925 954
Size: 211 778 190 792 2180
Fragments arranged by size:
2180 792 778 211 190
BsmBI CGTCTCn'nnnn_
Cuts at: 896 896
Size: 4151
Bsp1286I G_dGCh'C
Cuts at: 601 648 799 954 1165 1776 1869 1943 2133
Size: 47 151 155 211 611 93 74 190
Cuts at: 2133 2195 2925 601
Size: 62 730 1827
Fragments arranged by size:
1827 730 611 211 190 155 151 93
74 62 47
BspEI T'CCGG_A
Cuts at: 3371 3371
Size: 4151
BspHI T'CATG_A
Cuts at: 3327 3327
Size: 4151
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1201 1415 1717 2098 1201
Size: 214 302 381 3254
Fragments arranged by size:
3254 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2443 2497 3981 4146 50
Size: 2393 54 1484 165 55
Fragments arranged by size:
2393 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2063 2063
Size: 4151
BsrFI r'CCGG_y
Cuts at: 2149 2330 2539 2149
Size: 181 209 3761
Fragments arranged by size:
3761 209 181
BssSI C'ACGA_G
Cuts at: 2422 2780 2422
Size: 358 3793
BstAPI GCAn_nnn'nTGC
Cuts at: 1528 1528
Size: 4151
BstBI TT'CG_AA
Cuts at: 2512 2512
Size: 4151
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4151
BstYI r'GATC_y
Cuts at: 1 503 516 915 1176 1643 1666 2001 2247
Size: 502 13 399 261 467 23 335 246
Cuts at: 2247 3259 3345 3357 3703 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 502 467 449 399 346 335 261
246 86 23 13 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4151
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2101 2282 2445 2101
Size: 181 163 3807
Fragments arranged by size:
3807 181 163
BtsI GCAGTG_nn'
Cuts at: 1394 1394
Size: 4151
DraI TTT'AAA
Cuts at: 1150 1150
Size: 4151
DrdI GACnn_nn'nnGTC
Cuts at: 1040 1857 2715 1040
Size: 817 858 2476
Fragments arranged by size:
2476 858 817
EaeI y'GGCC_r
Cuts at: 581 817 962 1000 1736 1910 2301 2328 3673
Size: 236 145 38 736 174 391 27 1345
Cuts at: 3673 581
Size: 1059
Fragments arranged by size:
1345 1059 736 391 236 174 145 38
27
EagI C'GGCC_G
Cuts at: 1736 1736
Size: 4151
EarI CTCTTCn'nnn_
Cuts at: 476 2174 2384 476
Size: 1698 210 2243
Fragments arranged by size:
2243 1698 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2359 2669 2815 3961 2359
Size: 310 146 1146 2549
Fragments arranged by size:
2549 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1163 1163
Size: 4151
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 873 1976 2408 3155 3423 873
Size: 1103 432 747 268 1601
Fragments arranged by size:
1601 1103 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 163 163
Size: 4151
EcoO109I rG'GnC_Cy
Cuts at: 645 1290 645
Size: 645 3506
EcoRI G'AATT_C
Cuts at: 1155 1155
Size: 4151
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 171 755 2170 171
Size: 584 1415 2152
Fragments arranged by size:
2152 1415 584
FspI TGC'GCA
Cuts at: 1932 1932
Size: 4151
HaeII r_GCGC'y
Cuts at: 1585 1833 2855 3834 1585
Size: 248 1022 979 1902
Fragments arranged by size:
1902 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 109 141 1993 2023 2025 2055 109
Size: 32 1852 30 2 30 2205
Fragments arranged by size:
2205 1852 32 30 30 2
HincII GTy'rAC
Cuts at: 1190 1190
Size: 4151
KasI G'GCGC_C
Cuts at: 1581 1829 1581
Size: 248 3903
KpnI G_GTAC'C
Cuts at: 1171 1171
Size: 4151
MluI A'CGCG_T
Cuts at: 3584 3584
Size: 4151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2822 3006 2822
Size: 184 3967
MscI TGG'CCA
Cuts at: 583 1912 583
Size: 1329 2822
MslI CAynn'nnrTG
Cuts at: 463 1017 2140 2267 463
Size: 554 1123 127 2347
Fragments arranged by size:
2347 1123 554 127
MspA1I CmG'CkG
Cuts at: 1026 1262 1576 1936 2949 3194 1026
Size: 236 314 360 1013 245 1983
Fragments arranged by size:
1983 1013 360 314 245 236
NaeI GCC'GGC
Cuts at: 2332 2332
Size: 4151
NarI GG'CG_CC
Cuts at: 1582 1830 1582
Size: 248 3903
NcoI C'CATG_G
Cuts at: 107 107
Size: 4151
NgoMIV G'CCGG_C
Cuts at: 2330 2330
Size: 4151
NspI r_CATG'y
Cuts at: 462 696 828 1204 1704 2611 462
Size: 234 132 376 500 907 2002
Fragments arranged by size:
2002 907 500 376 234 132
PciI A'CATG_T
Cuts at: 458 692 824 2607 458
Size: 234 132 1783 2002
Fragments arranged by size:
2002 1783 234 132
PflMI CCAn_nnn'nTGG
Cuts at: 1015 1015
Size: 4151
PmeI GTTT'AAAC
Cuts at: 1150 1150
Size: 4151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3290 3322 3290
Size: 32 4119
PsiI TTA'TAA
Cuts at: 409 409
Size: 4151
PspOMI G'GGCC_C
Cuts at: 644 644
Size: 4151
PstI C_TGCA'G
Cuts at: 1198 1883 1198
Size: 685 3466
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4151
PvuII CAG'CTG
Cuts at: 1026 1576 1936 1026
Size: 550 360 3241
Fragments arranged by size:
3241 550 360
RsrII CG'GwC_CG
Cuts at: 2346 2346
Size: 4151
SacI G_AGCT'C
Cuts at: 1165 1165
Size: 4151
SalI G'TCGA_C
Cuts at: 1188 1188
Size: 4151
SapI GCTCTTCn'nnn_
Cuts at: 2174 2384 2174
Size: 210 3941
SbfI CC_TGCA'GG
Cuts at: 1198 1198
Size: 4151
ScaI AGT'ACT
Cuts at: 3668 3668
Size: 4151
SexAI A'CCwGG_T
Cuts at: 631 631
Size: 4151
SfcI C'TryA_G
Cuts at: 33 1194 1879 2872 3063 33
Size: 1161 685 993 191 1121
Fragments arranged by size:
1161 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1583 1831 1583
Size: 248 3903
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
SmaI CCC'GGG
Cuts at: 1173 1173
Size: 4151
SmlI C'TyrA_G
Cuts at: 285 1306 2221 2713 2975 285
Size: 1021 915 492 262 1461
Fragments arranged by size:
1461 1021 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4151
SphI G_CATG'C
Cuts at: 1204 1704 1204
Size: 500 3651
SspI AAT'ATT
Cuts at: 366 487 782 366
Size: 121 295 3735
Fragments arranged by size:
3735 295 121
StuI AGG'CCT
Cuts at: 1108 1108
Size: 4151
StyI C'CwwG_G
Cuts at: 107 159 1285 1639 107
Size: 52 1126 354 2619
Fragments arranged by size:
2619 1126 354 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1734 1867 2326 2535 1734
Size: 133 459 209 3350
Fragments arranged by size:
3350 459 209 133
TatI w'GTAC_w
Cuts at: 3666 3666
Size: 4151
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 663 695 3668 3700 663
Size: 32 2973 32 1114
Fragments arranged by size:
2973 1114 32 32
Tth111I GACn'n_nGTC
Cuts at: 1948 1948
Size: 4151
XbaI T'CTAG_A
Cuts at: 399 1182 399
Size: 783 3368
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1646 1646
Size: 4151
XmaI C'CCGG_G
Cuts at: 1171 1171
Size: 4151
XmnI GAAnn'nnTTC
Cuts at: 988 988
Size: 4151
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AleI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmtI BpmI Bpu10I BseRI
BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PpuMI PshAI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SwaI XhoI ZraI