Restriction map for
pF1KB3206
(Circular) (Six-base) MAPSORT of: pF1KB3206.seq Check: 2695 from: 1 to: 5498
pF1KB3206 5498 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1607 1607
Size: 5498
AatII G_ACGT'C
Cuts at: 1439 1439
Size: 5498
AccI GT'mk_AC
Cuts at: 2171 2536 2171
Size: 365 5133
Acc65I G'GTAC_C
Cuts at: 2514 2514
Size: 5498
AclI AA'CG_TT
Cuts at: 5338 5338
Size: 5498
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3323 3755 4108 4770 3323
Size: 432 353 662 4051
Fragments arranged by size:
4051 662 432 353
AflIII A'CryG_T
Cuts at: 2850 4650 4931 2850
Size: 1800 281 3417
Fragments arranged by size:
3417 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 1195 2803 4241 1195
Size: 1608 1438 2452
Fragments arranged by size:
2452 1608 1438
ApaI G_GGCC'C
Cuts at: 2299 2299
Size: 5498
ApaLI G'TGCA_C
Cuts at: 4336 4336
Size: 5498
ApoI r'AATT_y
Cuts at: 246 1109 2401 2502 4851 5107 246
Size: 863 1292 101 2349 256 637
Fragments arranged by size:
2349 1292 863 637 256 101
AseI AT'TA_AT
Cuts at: 20 812 989 20
Size: 792 177 4529
Fragments arranged by size:
4529 792 177
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5498
AvaI C'yCGr_G
Cuts at: 268 299 1545 1893 1942 2518 268
Size: 31 1246 348 49 576 3248
Fragments arranged by size:
3248 1246 576 348 49 31
BamHI G'GATC_C
Cuts at: 2523 2523
Size: 5498
BanI G'GyrC_C
Cuts at: 1549 1702 1833 2286 2329 2514 2928 3176 3211
Size: 153 131 453 43 185 414 248 35
Cuts at: 3211 1549
Size: 3836
Fragments arranged by size:
3836 453 414 248 185 153 131 43
35
BanII G_rGCy'C
Cuts at: 190 300 392 470 731 1943 2299 2512 3542
Size: 110 92 78 261 1212 356 213 1030
Cuts at: 3542 190
Size: 2146
Fragments arranged by size:
2146 1212 1030 356 261 213 110 92
78
BbeI G_GCGC'C
Cuts at: 1706 2932 3180 1706
Size: 1226 248 4024
Fragments arranged by size:
4024 1226 248
BbsI GAAGACnn'nnnn_
Cuts at: 278 495 278
Size: 217 5281
BciVI GTATCCnnnnn_n'
Cuts at: 1208 3396 4452 5498 1208
Size: 2188 1056 1046 1208
Fragments arranged by size:
2188 1208 1056 1046
BclI T'GATC_A
Cuts at: 489 3018 489
Size: 2529 2969
BglII A'GATC_T
Cuts at: 3013 5050 3013
Size: 2037 3461
BlpI GC'TnA_GC
Cuts at: 1996 2610 1996
Size: 614 4884
Bme1580I G_kGCm'C
Cuts at: 1552 1838 2299 3123 3216 4340 1552
Size: 286 461 824 93 1124 2710
Fragments arranged by size:
2710 1124 824 461 286 93
BmgBI CAC'GTC
Cuts at: 2322 2322
Size: 5498
BmrI ACTGGGnnnn_n'
Cuts at: 206 359 2710 2791 3126 5270 206
Size: 153 2351 81 335 2144 434
Fragments arranged by size:
2351 2144 434 335 153 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1068 3865 1068
Size: 2797 2701
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 883 1172 2367 2396 454
Size: 429 289 1195 29 3556
Fragments arranged by size:
3556 1195 429 289 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1912 2674 3553 4267 4565 1912
Size: 762 879 714 298 2845
Fragments arranged by size:
2845 879 762 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1320 1434 1623 5202 1320
Size: 114 189 3579 1616
Fragments arranged by size:
3579 1616 189 114
BsaAI yAC'GTr
Cuts at: 2085 2851 2085
Size: 766 4732
BsaBI GATnn'nnATC
Cuts at: 6 3012 5049 6
Size: 3006 2037 455
Fragments arranged by size:
3006 2037 455
BsaHI Gr'CG_yC
Cuts at: 1436 1703 1766 2929 3177 3879 5375 1436
Size: 267 63 1163 248 702 1496 1559
Fragments arranged by size:
1559 1496 1163 702 267 248 63
BsaWI w'CCGG_w
Cuts at: 2687 3208 3886 4297 4444 4718 2687
Size: 521 678 411 147 274 3467
Fragments arranged by size:
3467 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1702 1732 2089 2119 1702
Size: 30 357 30 5081
Fragments arranged by size:
5081 357 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 400 2268 400
Size: 1868 3630
BseYI C'CCAG_C
Cuts at: 168 2108 2923 3681 4346 4754 168
Size: 1940 815 758 665 408 912
Fragments arranged by size:
1940 912 815 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1495 1806 2065 2268 1495
Size: 311 259 203 4725
Fragments arranged by size:
4725 311 259 203
BsiEI CG_ry'CG
Cuts at: 104 1847 2273 2277 3040 3086 4316 104
Size: 1743 426 4 763 46 1230 1286
Fragments arranged by size:
1743 1286 1230 763 426 46 4
BsiHKAI G_wGCw'C
Cuts at: 392 470 731 1412 2090 2225 2443 2512 3290
Size: 78 261 681 678 135 218 69 778
Cuts at: 3290 3480 4340 392
Size: 190 860 1550
Fragments arranged by size:
1550 860 778 681 678 261 218 190
135 78 69
BsmBI CGTCTCn'nnnn_
Cuts at: 903 903
Size: 5498
Bsp1286I G_dGCh'C
Cuts at: 190 300 392 470 731 1412 1552 1838 1943
Size: 110 92 78 261 681 140 286 105
Cuts at: 1943 2090 2225 2299 2443 2512 3123 3216 3290
Size: 147 135 74 144 69 611 93 74
Cuts at: 3290 3480 3542 4340 190
Size: 190 62 798 1348
Fragments arranged by size:
1348 798 681 611 286 261 190 147
144 140 135 110 105 93 92 78
74 74 69 62
BspHI T'CATG_A
Cuts at: 3930 3930
Size: 5498
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1607 2126 2548 2762 3064 3445 1607
Size: 519 422 214 302 381 3660
Fragments arranged by size:
3660 519 422 381 302 214
BsrBI CCG'CTC
Cuts at: 50 230 3790 3844 5328 5493 50
Size: 180 3560 54 1484 165 55
Fragments arranged by size:
3560 1484 180 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3410 3410
Size: 5498
BsrFI r'CCGG_y
Cuts at: 3496 3677 3496
Size: 181 5317
BssSI C'ACGA_G
Cuts at: 3769 4477 3769
Size: 708 4790
BstAPI GCAn_nnn'nTGC
Cuts at: 150 2875 150
Size: 2725 2773
BstBI TT'CG_AA
Cuts at: 3859 3859
Size: 5498
BstEII G'GTnAC_C
Cuts at: 1863 1863
Size: 5498
BstXI CCAn_nnnn'nTGG
Cuts at: 1875 1875
Size: 5498
BstYI r'GATC_y
Cuts at: 1 2523 2990 3013 3348 3594 3900 3912 3998
Size: 2522 467 23 335 246 306 12 86
Cuts at: 3998 5050 1
Size: 1052 449
Fragments arranged by size:
2522 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1654 2291 1654
Size: 637 4861
BtgI C'CryG_G
Cuts at: 107 1713 1934 107
Size: 1606 221 3671
Fragments arranged by size:
3671 1606 221
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3448 3629 3792 3448
Size: 181 163 5154
Fragments arranged by size:
5154 181 163
BtsI GCAGTG_nn'
Cuts at: 743 2741 743
Size: 1998 3500
DraI TTT'AAA
Cuts at: 2497 2497
Size: 5498
DrdI GACnn_nn'nnGTC
Cuts at: 3204 4548 3204
Size: 1344 4154
EaeI y'GGCC_r
Cuts at: 1638 1844 2012 2066 3083 3257 3648 3675 5020
Size: 206 168 54 1017 174 391 27 1345
Cuts at: 5020 1638
Size: 2116
Fragments arranged by size:
2116 1345 1017 391 206 174 168 54
27
EagI C'GGCC_G
Cuts at: 1844 3083 1844
Size: 1239 4259
EarI CTCTTCn'nnn_
Cuts at: 710 2212 2218 3521 3731 710
Size: 1502 6 1303 210 2477
Fragments arranged by size:
2477 1502 1303 210 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3706 4448 4594 5308 3706
Size: 742 146 714 3896
Fragments arranged by size:
3896 742 714 146
EcoICRI GAG'CTC
Cuts at: 390 468 729 2510 390
Size: 78 261 1781 3378
Fragments arranged by size:
3378 1781 261 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 883 1172 2367 2396 3323 3755 4108 4770
Size: 429 289 1195 29 927 432 353 662
Cuts at: 4770 454
Size: 1182
Fragments arranged by size:
1195 1182 927 662 432 429 353 289
29
EcoNI CCTnn'n_nnAGG
Cuts at: 789 789
Size: 5498
EcoO109I rG'GnC_Cy
Cuts at: 761 1388 1590 1674 2295 2296 2336 2637 761
Size: 627 202 84 621 1 40 301 3622
Fragments arranged by size:
3622 627 621 301 202 84 40 1
EcoRI G'AATT_C
Cuts at: 1109 2502 1109
Size: 1393 4105
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 169 3517 169
Size: 3348 2150
FspI TGC'GCA
Cuts at: 1981 3279 1981
Size: 1298 4200
FspAI rTGC'GCAy
Cuts at: 1981 1981
Size: 5498
HaeII r_GCGC'y
Cuts at: 706 723 1532 1706 1744 2932 3180 4410 5181
Size: 17 809 174 38 1188 248 1230 771
Cuts at: 5181 706
Size: 1023
Fragments arranged by size:
1230 1188 1023 809 771 248 174 38
17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 745 777 901 933 1213 1245 1528 1560 1702
Size: 32 124 32 280 32 283 32 142
Cuts at: 1702 1734 1876 1908 2089 2121 3340 3370 3372
Size: 32 142 32 181 32 1219 30 2
Cuts at: 3372 3402 745
Size: 30 2841
Fragments arranged by size:
2841 1219 283 280 181 142 142 124
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1566 2537 1566
Size: 971 4527
KasI G'GCGC_C
Cuts at: 1702 2928 3176 1702
Size: 1226 248 4024
Fragments arranged by size:
4024 1226 248
KpnI G_GTAC'C
Cuts at: 2518 2518
Size: 5498
MfeI C'AATT_G
Cuts at: 1040 1040
Size: 5498
MluI A'CGCG_T
Cuts at: 4931 4931
Size: 5498
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1457 4257 4441 1457
Size: 2800 184 2514
Fragments arranged by size:
2800 2514 184
MscI TGG'CCA
Cuts at: 3259 3259
Size: 5498
MslI CAynn'nnrTG
Cuts at: 1420 3487 3614 1420
Size: 2067 127 3304
Fragments arranged by size:
3304 2067 127
MspA1I CmG'CkG
Cuts at: 336 1332 1800 1923 2609 2923 3283 4067 4312
Size: 996 468 123 686 314 360 784 245
Cuts at: 4312 336
Size: 1522
Fragments arranged by size:
1522 996 784 686 468 360 314 245
123
NaeI GCC'GGC
Cuts at: 3679 3679
Size: 5498
NarI GG'CG_CC
Cuts at: 1703 2929 3177 1703
Size: 1226 248 4024
Fragments arranged by size:
4024 1226 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5498
NgoMIV G'CCGG_C
Cuts at: 3677 3677
Size: 5498
NspI r_CATG'y
Cuts at: 2551 3051 4654 2551
Size: 500 1603 3395
Fragments arranged by size:
3395 1603 500
PasI CC'CwG_GG
Cuts at: 261 1838 261
Size: 1577 3921
PciI A'CATG_T
Cuts at: 4650 4650
Size: 5498
PfoI T'CCnGG_A
Cuts at: 1262 2126 2304 1262
Size: 864 178 4456
Fragments arranged by size:
4456 864 178
PmeI GTTT'AAAC
Cuts at: 2497 2497
Size: 5498
PmlI CAC'GTG
Cuts at: 2085 2085
Size: 5498
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3944 3976 3944
Size: 32 5466
PpuMI rG'GwC_Cy
Cuts at: 761 1388 1590 2336 761
Size: 627 202 746 3923
Fragments arranged by size:
3923 746 627 202
PspOMI G'GGCC_C
Cuts at: 2295 2295
Size: 5498
PspXI vC'TCGA_Gb
Cuts at: 268 268
Size: 5498
PstI C_TGCA'G
Cuts at: 137 1682 1805 2545 3230 137
Size: 1545 123 740 685 2405
Fragments arranged by size:
2405 1545 740 685 123
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5498
PvuII CAG'CTG
Cuts at: 336 1923 2923 3283 336
Size: 1587 1000 360 2551
Fragments arranged by size:
2551 1587 1000 360
RsrII CG'GwC_CG
Cuts at: 3693 3693
Size: 5498
SacI G_AGCT'C
Cuts at: 392 470 731 2512 392
Size: 78 261 1781 3378
Fragments arranged by size:
3378 1781 261 78
SalI G'TCGA_C
Cuts at: 2535 2535
Size: 5498
SanDI GG'GwC_CC
Cuts at: 761 1388 2336 761
Size: 627 948 3923
Fragments arranged by size:
3923 948 627
SapI GCTCTTCn'nnn_
Cuts at: 710 2212 3521 3731 710
Size: 1502 1309 210 2477
Fragments arranged by size:
2477 1502 1309 210
SbfI CC_TGCA'GG
Cuts at: 1682 2545 1682
Size: 863 4635
ScaI AGT'ACT
Cuts at: 5015 5015
Size: 5498
SexAI A'CCwGG_T
Cuts at: 1859 2174 1859
Size: 315 5183
SfcI C'TryA_G
Cuts at: 33 133 1100 1678 1801 2541 3226 4194 4385
Size: 100 967 578 123 740 685 968 191
Cuts at: 4385 33
Size: 1146
Fragments arranged by size:
1146 968 967 740 685 578 191 123
100
SfoI GGC'GCC
Cuts at: 1704 2930 3178 1704
Size: 1226 248 4024
Fragments arranged by size:
4024 1226 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5498
SmaI CCC'GGG
Cuts at: 1944 2520 1944
Size: 576 4922
SmlI C'TyrA_G
Cuts at: 268 1927 2653 3568 4282 4544 268
Size: 1659 726 915 714 262 1222
Fragments arranged by size:
1659 1222 915 726 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5498
SphI G_CATG'C
Cuts at: 2551 3051 2551
Size: 500 4998
SrfI GCCC'GGGC
Cuts at: 1944 1944
Size: 5498
StyI C'CwwG_G
Cuts at: 107 785 876 1868 2632 2986 107
Size: 678 91 992 764 354 2619
Fragments arranged by size:
2619 992 764 678 354 91
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1477 2263 3081 3214 3673 3882 1477
Size: 786 818 133 459 209 3093
Fragments arranged by size:
3093 818 786 459 209 133
TatI w'GTAC_w
Cuts at: 1355 5013 1355
Size: 3658 1840
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 257 289 609 641 2300 2332 5015 5047 257
Size: 32 320 32 1659 32 2683 32 708
Fragments arranged by size:
2683 1659 708 320 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2340 3295 2340
Size: 955 4543
XbaI T'CTAG_A
Cuts at: 2529 2529
Size: 5498
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2022 2993 2022
Size: 971 4527
XhoI C'TCGA_G
Cuts at: 268 268
Size: 5498
XmaI C'CCGG_G
Cuts at: 1942 2518 1942
Size: 576 4922
XmnI GAAnn'nnTTC
Cuts at: 968 968
Size: 5498
ZraI GAC'GTC
Cuts at: 1437 1437
Size: 5498
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmtI Bpu10I
BsiWI BsmI BspEI BsrGI BssHII BstZ17I ClaI CspCI
DraIII EcoRV FseI HindIII HpaI NdeI NheI NotI
NruI NsiI PacI PflMI PshAI PsiI PsrI SacII
SfiI SgrAI SnaBI SspI StuI SwaI