Restriction map for
pF1KB3214
(Circular) (Six-base) MAPSORT of: pF1KB3214.seq Check: 6782 from: 1 to: 5636
pF1KB3214 5636 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 2674 2674
Size: 5636
Acc65I G'GTAC_C
Cuts at: 1611 2652 1611
Size: 1041 4595
AclI AA'CG_TT
Cuts at: 5476 5476
Size: 5636
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 468 3461 3893 4246 4908 468
Size: 2993 432 353 662 1196
Fragments arranged by size:
2993 1196 662 432 353
AfeI AGC'GCT
Cuts at: 1416 2092 1416
Size: 676 4960
AflII C'TTAA_G
Cuts at: 1128 1128
Size: 5636
AflIII A'CryG_T
Cuts at: 776 2988 4788 5069 776
Size: 2212 1800 281 1343
Fragments arranged by size:
2212 1800 1343 281
AhdI GACnn_n'nnGTC
Cuts at: 1911 1911
Size: 5636
AleI CACnn'nnGTG
Cuts at: 1438 1438
Size: 5636
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1214 1456 2387 1214
Size: 242 931 4463
Fragments arranged by size:
4463 931 242
AlwNI CAG_nnn'CTG
Cuts at: 303 507 1673 2283 2941 4379 303
Size: 204 1166 610 658 1438 1560
Fragments arranged by size:
1560 1438 1166 658 610 204
ApaI G_GGCC'C
Cuts at: 258 258
Size: 5636
ApaLI G'TGCA_C
Cuts at: 4474 4474
Size: 5636
ApoI r'AATT_y
Cuts at: 2124 2640 4989 5245 2124
Size: 516 2349 256 2515
Fragments arranged by size:
2515 2349 516 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5636
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5636
AvaI C'yCGr_G
Cuts at: 730 2266 2508 2656 730
Size: 1536 242 148 3710
Fragments arranged by size:
3710 1536 242 148
BamHI G'GATC_C
Cuts at: 2661 2661
Size: 5636
BanI G'GyrC_C
Cuts at: 1491 1611 2451 2652 3066 3314 3349 1491
Size: 120 840 201 414 248 35 3778
Fragments arranged by size:
3778 840 414 248 201 120 35
BanII G_rGCy'C
Cuts at: 258 674 766 1714 2266 2650 3680 258
Size: 416 92 948 552 384 1030 2214
Fragments arranged by size:
2214 1030 948 552 416 384 92
BbeI G_GCGC'C
Cuts at: 3070 3318 3070
Size: 248 5388
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 565 599 565
Size: 34 5602
BciVI GTATCCnnnnn_n'
Cuts at: 3534 4590 5636 3534
Size: 1056 1046 3534
Fragments arranged by size:
3534 1056 1046
BclI T'GATC_A
Cuts at: 3156 3156
Size: 5636
BfrBI ATG'CAT
Cuts at: 841 2201 841
Size: 1360 4276
BglI GCCn_nnn'nGGC
Cuts at: 262 640 262
Size: 378 5258
BglII A'GATC_T
Cuts at: 1977 3151 5188 1977
Size: 1174 2037 2425
Fragments arranged by size:
2425 2037 1174
BlpI GC'TnA_GC
Cuts at: 2360 2748 2360
Size: 388 5248
Bme1580I G_kGCm'C
Cuts at: 258 746 2408 2454 3261 3354 4478 258
Size: 488 1662 46 807 93 1124 1416
Fragments arranged by size:
1662 1416 1124 807 488 93 46
BmgBI CAC'GTC
Cuts at: 222 741 222
Size: 519 5117
BmrI ACTGGGnnnn_n'
Cuts at: 941 1172 2848 2929 3264 5408 941
Size: 231 1676 81 335 2144 1169
Fragments arranged by size:
2144 1676 1169 335 231 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1761 2549 4003 1761
Size: 788 1454 3394
Fragments arranged by size:
3394 1454 788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 1010 1114 1395 1857 2316 490
Size: 520 104 281 462 459 3810
Fragments arranged by size:
3810 520 462 459 281 104
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 889 2812 3691 4405 4703 889
Size: 1923 879 714 298 1822
Fragments arranged by size:
1923 1822 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1139 1642 5340 1139
Size: 503 3698 1435
Fragments arranged by size:
3698 1435 503
BsaAI yAC'GTr
Cuts at: 1296 2989 1296
Size: 1693 3943
BsaBI GATnn'nnATC
Cuts at: 6 3150 5187 6
Size: 3144 2037 455
Fragments arranged by size:
3144 2037 455
BsaHI Gr'CG_yC
Cuts at: 1004 3067 3315 4017 5513 1004
Size: 2063 248 702 1496 1127
Fragments arranged by size:
2063 1496 1127 702 248
BsaWI w'CCGG_w
Cuts at: 1037 1388 1885 2825 3346 4024 4435 4582 4856
Size: 351 497 940 521 678 411 147 274
Cuts at: 4856 1037
Size: 1817
Fragments arranged by size:
1817 940 678 521 497 411 351 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2209 2239 2209
Size: 30 5606
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 361 607 610 622 625 1398 1407 1734 1737
Size: 246 3 12 3 773 9 327 3
Cuts at: 1737 2502 2525 361
Size: 765 23 3472
Fragments arranged by size:
3472 773 765 327 246 23 12 9
3 3 3
BseYI C'CCAG_C
Cuts at: 500 1048 1074 1078 1531 2087 2455 3061 3819
Size: 548 26 4 453 556 368 606 758
Cuts at: 3819 4484 4892 500
Size: 665 408 1244
Fragments arranged by size:
1244 758 665 606 556 548 453 408
368 26 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 150 1945 150
Size: 1795 3841
BsiEI CG_ry'CG
Cuts at: 104 3178 3224 4454 104
Size: 3074 46 1230 1286
Fragments arranged by size:
3074 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 766 1435 2650 3428 3618 4478 766
Size: 669 1215 778 190 860 1924
Fragments arranged by size:
1924 1215 860 778 669 190
BsmI GAATG_Cn'
Cuts at: 1443 1443
Size: 5636
BsmBI CGTCTCn'nnnn_
Cuts at: 607 622 607
Size: 15 5621
Bsp1286I G_dGCh'C
Cuts at: 258 674 746 766 1435 1714 2266 2408 2454
Size: 416 72 20 669 279 552 142 46
Cuts at: 2454 2650 3261 3354 3428 3618 3680 4478 258
Size: 196 611 93 74 190 62 798 1416
Fragments arranged by size:
1416 798 669 611 552 416 279 196
190 142 93 74 72 62 46 20
BspEI T'CCGG_A
Cuts at: 1885 1885
Size: 5636
BspHI T'CATG_A
Cuts at: 2049 4068 2049
Size: 2019 3617
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2686 2900 3202 3583 2686
Size: 214 302 381 4739
Fragments arranged by size:
4739 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1018 1706 3928 3982 5466 5631 50
Size: 968 688 2222 54 1484 165 55
Fragments arranged by size:
2222 1484 968 688 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2369 3548 2369
Size: 1179 4457
BsrFI r'CCGG_y
Cuts at: 1488 1516 3634 3815 1488
Size: 28 2118 181 3309
Fragments arranged by size:
3309 2118 181 28
BsrGI T'GTAC_A
Cuts at: 1820 2596 1820
Size: 776 4860
BssSI C'ACGA_G
Cuts at: 565 3907 4615 565
Size: 3342 708 1586
Fragments arranged by size:
3342 1586 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3013 3013
Size: 5636
BstBI TT'CG_AA
Cuts at: 3997 3997
Size: 5636
BstEII G'GTnAC_C
Cuts at: 1243 1243
Size: 5636
BstXI CCAn_nnnn'nTGG
Cuts at: 114 1110 114
Size: 996 4640
BstYI r'GATC_y
Cuts at: 1 1099 1977 2273 2661 3128 3151 3486 3732
Size: 1098 878 296 388 467 23 335 246
Cuts at: 3732 4038 4050 4136 5188 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1098 1052 878 467 449 388 335 306
296 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 344 858 1339 344
Size: 514 481 4641
Fragments arranged by size:
4641 514 481
BtgI C'CryG_G
Cuts at: 1067 1325 1551 2312 2324 2564 1067
Size: 258 226 761 12 240 4139
Fragments arranged by size:
4139 761 258 240 226 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2064 3586 3767 3930 2064
Size: 1522 181 163 3770
Fragments arranged by size:
3770 1522 181 163
BtsI GCAGTG_nn'
Cuts at: 162 288 392 2396 2879 162
Size: 126 104 2004 483 2919
Fragments arranged by size:
2919 2004 483 126 104
DraI TTT'AAA
Cuts at: 2635 2635
Size: 5636
DrdI GACnn_nn'nnGTC
Cuts at: 3342 4686 3342
Size: 1344 4292
EaeI y'GGCC_r
Cuts at: 272 632 1518 1565 3221 3395 3786 3813 5158
Size: 360 886 47 1656 174 391 27 1345
Cuts at: 5158 272
Size: 750
Fragments arranged by size:
1656 1345 886 750 391 360 174 47
27
EagI C'GGCC_G
Cuts at: 3221 3221
Size: 5636
EarI CTCTTCn'nnn_
Cuts at: 599 947 959 1683 3659 3869 599
Size: 348 12 724 1976 210 2366
Fragments arranged by size:
2366 1976 724 348 210 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3844 4586 4732 5446 3844
Size: 742 146 714 4034
Fragments arranged by size:
4034 742 714 146
EcoICRI GAG'CTC
Cuts at: 764 2648 764
Size: 1884 3752
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 468 490 1010 1114 1395 1857 2316 3461 3893
Size: 22 520 104 281 462 459 1145 432
Cuts at: 3893 4246 4908 468
Size: 353 662 1196
Fragments arranged by size:
1196 1145 662 520 462 459 432 353
281 104 22
EcoNI CCTnn'n_nnAGG
Cuts at: 1128 1271 2236 2484 1128
Size: 143 965 248 4280
Fragments arranged by size:
4280 965 248 143
EcoO109I rG'GnC_Cy
Cuts at: 1345 2280 2530 2775 1345
Size: 935 250 245 4206
Fragments arranged by size:
4206 935 250 245
EcoRI G'AATT_C
Cuts at: 2640 2640
Size: 5636
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3655 3655
Size: 5636
FspI TGC'GCA
Cuts at: 457 2388 3417 457
Size: 1931 1029 2676
Fragments arranged by size:
2676 1931 1029
HaeII r_GCGC'y
Cuts at: 304 561 1418 2094 3070 3318 4548 5319 304
Size: 257 857 676 976 248 1230 771 621
Fragments arranged by size:
1230 976 857 771 676 621 257 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 407 439 1897 1929 2209 2241 3478 3508 3510
Size: 32 1458 32 280 32 1237 30 2
Cuts at: 3510 3540 407
Size: 30 2503
Fragments arranged by size:
2503 1458 1237 280 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 782 2675 782
Size: 1893 3743
HpaI GTT'AAC
Cuts at: 782 782
Size: 5636
KasI G'GCGC_C
Cuts at: 3066 3314 3066
Size: 248 5388
KpnI G_GTAC'C
Cuts at: 1615 2656 1615
Size: 1041 4595
MluI A'CGCG_T
Cuts at: 776 5069 776
Size: 4293 1343
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 288 1334 1340 2082 2195 4395 4579 288
Size: 1046 6 742 113 2200 184 1345
Fragments arranged by size:
2200 1345 1046 742 184 113 6
MscI TGG'CCA
Cuts at: 3397 3397
Size: 5636
MslI CAynn'nnrTG
Cuts at: 112 142 1438 3625 3752 112
Size: 30 1296 2187 127 1996
Fragments arranged by size:
2187 1996 1296 127 30
MspA1I CmG'CkG
Cuts at: 198 375 440 1078 1604 2747 3061 3421 4205
Size: 177 65 638 526 1143 314 360 784
Cuts at: 4205 4450 198
Size: 245 1384
Fragments arranged by size:
1384 1143 784 638 526 360 314 245
177 65
NaeI GCC'GGC
Cuts at: 1518 3817 1518
Size: 2299 3337
NarI GG'CG_CC
Cuts at: 3067 3315 3067
Size: 248 5388
NcoI C'CATG_G
Cuts at: 1325 2312 1325
Size: 987 4649
NgoMIV G'CCGG_C
Cuts at: 1516 3815 1516
Size: 2299 3337
NsiI A_TGCA'T
Cuts at: 843 2203 843
Size: 1360 4276
NspI r_CATG'y
Cuts at: 281 462 821 2201 2689 3189 4792 281
Size: 181 359 1380 488 500 1603 1125
Fragments arranged by size:
1603 1380 1125 500 488 359 181
PasI CC'CwG_GG
Cuts at: 650 1761 2447 650
Size: 1111 686 3839
Fragments arranged by size:
3839 1111 686
PciI A'CATG_T
Cuts at: 4788 4788
Size: 5636
PfoI T'CCnGG_A
Cuts at: 878 1102 878
Size: 224 5412
PmeI GTTT'AAAC
Cuts at: 2635 2635
Size: 5636
PmlI CAC'GTG
Cuts at: 1296 1296
Size: 5636
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4082 4114 4082
Size: 32 5604
PpuMI rG'GwC_Cy
Cuts at: 2530 2530
Size: 5636
PspOMI G'GGCC_C
Cuts at: 254 254
Size: 5636
PstI C_TGCA'G
Cuts at: 497 588 1723 2683 3368 497
Size: 91 1135 960 685 2765
Fragments arranged by size:
2765 1135 960 685 91
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5636
PvuII CAG'CTG
Cuts at: 1078 1604 3061 3421 1078
Size: 526 1457 360 3293
Fragments arranged by size:
3293 1457 526 360
RsrII CG'GwC_CG
Cuts at: 3831 3831
Size: 5636
SacI G_AGCT'C
Cuts at: 766 2650 766
Size: 1884 3752
SalI G'TCGA_C
Cuts at: 2673 2673
Size: 5636
SapI GCTCTTCn'nnn_
Cuts at: 1683 3659 3869 1683
Size: 1976 210 3450
Fragments arranged by size:
3450 1976 210
SbfI CC_TGCA'GG
Cuts at: 2683 2683
Size: 5636
ScaI AGT'ACT
Cuts at: 5153 5153
Size: 5636
SfcI C'TryA_G
Cuts at: 33 493 584 1719 1961 2679 3364 4332 4523
Size: 460 91 1135 242 718 685 968 191
Cuts at: 4523 33
Size: 1146
Fragments arranged by size:
1146 1135 968 718 685 460 242 191
91
SfoI GGC'GCC
Cuts at: 3068 3316 3068
Size: 248 5388
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5636
SmaI CCC'GGG
Cuts at: 2510 2658 2510
Size: 148 5488
SmlI C'TyrA_G
Cuts at: 904 1128 2791 3706 4420 4682 904
Size: 224 1663 915 714 262 1858
Fragments arranged by size:
1858 1663 915 714 262 224
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5636
SphI G_CATG'C
Cuts at: 281 462 2201 2689 3189 281
Size: 181 1739 488 500 2728
Fragments arranged by size:
2728 1739 500 488 181
SspI AAT'ATT
Cuts at: 289 289
Size: 5636
StuI AGG'CCT
Cuts at: 1543 1802 1543
Size: 259 5377
StyI C'CwwG_G
Cuts at: 755 1007 1325 1643 1984 2312 2351 2770 3124
Size: 252 318 318 341 328 39 419 354
Cuts at: 3124 755
Size: 3267
Fragments arranged by size:
3267 419 354 341 328 318 318 252
39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1497 2344 2469 3219 3352 3811 4020 1497
Size: 847 125 750 133 459 209 3113
Fragments arranged by size:
3113 847 750 459 209 133 125
TatI w'GTAC_w
Cuts at: 1180 1820 2596 5151 1180
Size: 640 776 2555 1665
Fragments arranged by size:
2555 1665 776 640
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 535 567 1965 1997 5153 5185 535
Size: 32 1398 32 3156 32 986
Fragments arranged by size:
3156 1398 986 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3433 3433
Size: 5636
XbaI T'CTAG_A
Cuts at: 2667 2667
Size: 5636
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 177 1323 1502 3131 177
Size: 1146 179 1629 2682
Fragments arranged by size:
2682 1629 1146 179
XmaI C'CCGG_G
Cuts at: 2508 2656 2508
Size: 148 5488
XmnI GAAnn'nnTTC
Cuts at: 247 1993 247
Size: 1746 3890
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AgeI AloI AscI AvrII BaeI BbsI
BbvCI BmtI Bpu10I BsiWI BssHII BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII MfeI NdeI NheI
NotI NruI PacI PflMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI
XhoI ZraI