Restriction map for
pF1KB3218
(Circular) (Six-base) MAPSORT of: pF1KB3218.seq Check: 6610 from: 1 to: 5801
pF1KB3218 5801 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 683 1476 1916 2839 683
Size: 793 440 923 3645
Fragments arranged by size:
3645 923 793 440
Acc65I G'GTAC_C
Cuts at: 2817 2817
Size: 5801
AclI AA'CG_TT
Cuts at: 2167 5641 2167
Size: 3474 2327
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 506 1525 2067 2271 2628 2681 3626 4058 4411
Size: 1019 542 204 357 53 945 432 353
Cuts at: 4411 5073 506
Size: 662 1234
Fragments arranged by size:
1234 1019 945 662 542 432 357 353
204 53
AflII C'TTAA_G
Cuts at: 1174 1174
Size: 5801
AflIII A'CryG_T
Cuts at: 1731 3153 4953 5234 1731
Size: 1422 1800 281 2298
Fragments arranged by size:
2298 1800 1422 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2735 2735
Size: 5801
AlwNI CAG_nnn'CTG
Cuts at: 3106 4544 3106
Size: 1438 4363
ApaLI G'TGCA_C
Cuts at: 689 4639 689
Size: 3950 1851
ApoI r'AATT_y
Cuts at: 322 2805 5154 5410 322
Size: 2483 2349 256 713
Fragments arranged by size:
2483 2349 713 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5801
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5801
AvaI C'yCGr_G
Cuts at: 2821 2821
Size: 5801
BamHI G'GATC_C
Cuts at: 2826 2826
Size: 5801
BanI G'GyrC_C
Cuts at: 735 2817 3231 3479 3514 735
Size: 2082 414 248 35 3022
Fragments arranged by size:
3022 2082 414 248 35
BanII G_rGCy'C
Cuts at: 467 1766 2344 2815 3845 467
Size: 1299 578 471 1030 2423
Fragments arranged by size:
2423 1299 1030 578 471
BbeI G_GCGC'C
Cuts at: 3235 3483 3235
Size: 248 5553
BbsI GAAGACnn'nnnn_
Cuts at: 2753 2753
Size: 5801
BbvCI CC'TCA_GC
Cuts at: 1367 1693 1367
Size: 326 5475
BciVI GTATCCnnnnn_n'
Cuts at: 316 777 3699 4755 5801 316
Size: 461 2922 1056 1046 316
Fragments arranged by size:
2922 1056 1046 461 316
BclI T'GATC_A
Cuts at: 3321 3321
Size: 5801
BglII A'GATC_T
Cuts at: 1424 3316 5353 1424
Size: 1892 2037 1872
Fragments arranged by size:
2037 1892 1872
BlpI GC'TnA_GC
Cuts at: 461 1468 2913 461
Size: 1007 1445 3349
Fragments arranged by size:
3349 1445 1007
Bme1580I G_kGCm'C
Cuts at: 357 693 3426 3519 4643 357
Size: 336 2733 93 1124 1515
Fragments arranged by size:
2733 1515 1124 336 93
BmrI ACTGGGnnnn_n'
Cuts at: 523 3013 3094 3429 5573 523
Size: 2490 81 335 2144 751
Fragments arranged by size:
2490 2144 751 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4168 4168
Size: 5801
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 710 715 1351 1766 710
Size: 5 636 415 4745
Fragments arranged by size:
4745 636 415 5
Bpu10I CC'TnA_GC
Cuts at: 1367 1693 1367
Size: 326 5475
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1327 1905 2977 3856 4570 4868 1327
Size: 578 1072 879 714 298 2260
Fragments arranged by size:
2260 1072 879 714 578 298
BsaI GGTCTCn'nnnn_
Cuts at: 5505 5505
Size: 5801
BsaAI yAC'GTr
Cuts at: 3154 3154
Size: 5801
BsaBI GATnn'nnATC
Cuts at: 6 3315 5352 6
Size: 3309 2037 455
Fragments arranged by size:
3309 2037 455
BsaHI Gr'CG_yC
Cuts at: 3232 3480 4182 5678 3232
Size: 248 702 1496 3355
Fragments arranged by size:
3355 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2990 3511 4189 4600 4747 5021 2990
Size: 521 678 411 147 274 3770
Fragments arranged by size:
3770 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1280 1310 1280
Size: 30 5771
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 995 1348 1384 1516 995
Size: 353 36 132 5280
Fragments arranged by size:
5280 353 132 36
BseYI C'CCAG_C
Cuts at: 122 1325 2412 3226 3984 4649 5057 122
Size: 1203 1087 814 758 665 408 866
Fragments arranged by size:
1203 1087 866 814 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1662 2153 1662
Size: 491 5310
BsiEI CG_ry'CG
Cuts at: 104 3343 3389 4619 104
Size: 3239 46 1230 1286
Fragments arranged by size:
3239 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 467 693 2344 2815 3593 3783 4643 467
Size: 226 1651 471 778 190 860 1625
Fragments arranged by size:
1651 1625 860 778 471 226 190
BsmI GAATG_Cn'
Cuts at: 647 1658 647
Size: 1011 4790
Bsp1286I G_dGCh'C
Cuts at: 357 467 693 1766 2344 2815 3426 3519 3593
Size: 110 226 1073 578 471 611 93 74
Cuts at: 3593 3783 3845 4643 357
Size: 190 62 798 1515
Fragments arranged by size:
1515 1073 798 611 578 471 226 190
110 93 74 62
BspHI T'CATG_A
Cuts at: 417 4233 417
Size: 3816 1985
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1275 2851 3065 3367 3748 1275
Size: 1576 214 302 381 3328
Fragments arranged by size:
3328 1576 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4093 4147 5631 5796 50
Size: 4043 54 1484 165 55
Fragments arranged by size:
4043 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3713 3713
Size: 5801
BsrFI r'CCGG_y
Cuts at: 3799 3980 3799
Size: 181 5620
BssSI C'ACGA_G
Cuts at: 4072 4780 4072
Size: 708 5093
BstAPI GCAn_nnn'nTGC
Cuts at: 3178 3178
Size: 5801
BstBI TT'CG_AA
Cuts at: 4162 4162
Size: 5801
BstXI CCAn_nnnn'nTGG
Cuts at: 678 678
Size: 5801
BstYI r'GATC_y
Cuts at: 1 1221 1424 1902 2058 2316 2473 2562 2826
Size: 1220 203 478 156 258 157 89 264
Cuts at: 2826 3293 3316 3651 3897 4203 4215 4301 5353
Size: 467 23 335 246 306 12 86 1052
Cuts at: 5353 1
Size: 449
Fragments arranged by size:
1220 1052 478 467 449 335 306 264
258 246 203 157 156 89 86 23
12
BstZ17I GTA'TAC
Cuts at: 684 684
Size: 5801
BtgI C'CryG_G
Cuts at: 107 469 107
Size: 362 5439
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3751 3932 4095 3751
Size: 181 163 5457
Fragments arranged by size:
5457 181 163
BtsI GCAGTG_nn'
Cuts at: 1713 1995 2416 3044 1713
Size: 282 421 628 4470
Fragments arranged by size:
4470 628 421 282
ClaI AT'CG_AT
Cuts at: 2024 2024
Size: 5801
DraI TTT'AAA
Cuts at: 2508 2800 2508
Size: 292 5509
DrdI GACnn_nn'nnGTC
Cuts at: 3507 4851 3507
Size: 1344 4457
EaeI y'GGCC_r
Cuts at: 3386 3560 3951 3978 5323 3386
Size: 174 391 27 1345 3864
Fragments arranged by size:
3864 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3386 3386
Size: 5801
EarI CTCTTCn'nnn_
Cuts at: 600 1392 1457 1544 2702 3824 4034 600
Size: 792 65 87 1158 1122 210 2367
Fragments arranged by size:
2367 1158 1122 792 210 87 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4009 4751 4897 5611 4009
Size: 742 146 714 4199
Fragments arranged by size:
4199 742 714 146
EcoICRI GAG'CTC
Cuts at: 465 2342 2813 465
Size: 1877 471 3453
Fragments arranged by size:
3453 1877 471
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 506 710 715 1351 1525 1766 2067 2271 2628
Size: 204 5 636 174 241 301 204 357
Cuts at: 2628 2681 3626 4058 4411 5073 506
Size: 53 945 432 353 662 1234
Fragments arranged by size:
1234 945 662 636 432 357 353 301
241 204 204 174 53 5
EcoNI CCTnn'n_nnAGG
Cuts at: 2048 2534 2048
Size: 486 5315
EcoO109I rG'GnC_Cy
Cuts at: 1170 2940 1170
Size: 1770 4031
EcoRI G'AATT_C
Cuts at: 322 2805 322
Size: 2483 3318
EcoRV GAT'ATC
Cuts at: 597 2786 597
Size: 2189 3612
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3820 3820
Size: 5801
FspI TGC'GCA
Cuts at: 3582 3582
Size: 5801
HaeII r_GCGC'y
Cuts at: 3235 3483 4713 5484 3235
Size: 248 1230 771 3552
Fragments arranged by size:
3552 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 833 865 1374 1406 2371 2403 2421 2453 3643
Size: 32 509 32 965 32 18 32 1190
Cuts at: 3643 3673 3675 3705 833
Size: 30 2 30 2929
Fragments arranged by size:
2929 1190 965 509 32 32 32 32
30 30 18 2
HincII GTy'rAC
Cuts at: 1137 2307 2840 1137
Size: 1170 533 4098
Fragments arranged by size:
4098 1170 533
HindIII A'AGCT_T
Cuts at: 934 934
Size: 5801
KasI G'GCGC_C
Cuts at: 3231 3479 3231
Size: 248 5553
KpnI G_GTAC'C
Cuts at: 2821 2821
Size: 5801
MluI A'CGCG_T
Cuts at: 5234 5234
Size: 5801
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1511 2464 4560 4744 1511
Size: 953 2096 184 2568
Fragments arranged by size:
2568 2096 953 184
MscI TGG'CCA
Cuts at: 3562 3562
Size: 5801
MslI CAynn'nnrTG
Cuts at: 3790 3917 3790
Size: 127 5674
MspA1I CmG'CkG
Cuts at: 460 1329 1846 2912 3226 3586 4370 4615 460
Size: 869 517 1066 314 360 784 245 1646
Fragments arranged by size:
1646 1066 869 784 517 360 314 245
NaeI GCC'GGC
Cuts at: 3982 3982
Size: 5801
NarI GG'CG_CC
Cuts at: 3232 3480 3232
Size: 248 5553
NcoI C'CATG_G
Cuts at: 107 469 107
Size: 362 5439
NgoMIV G'CCGG_C
Cuts at: 3980 3980
Size: 5801
NspI r_CATG'y
Cuts at: 353 1735 2854 3354 4957 353
Size: 1382 1119 500 1603 1197
Fragments arranged by size:
1603 1382 1197 1119 500
PciI A'CATG_T
Cuts at: 1731 4953 1731
Size: 3222 2579
PflMI CCAn_nnn'nTGG
Cuts at: 535 2035 535
Size: 1500 4301
PmeI GTTT'AAAC
Cuts at: 2800 2800
Size: 5801
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4247 4279 4247
Size: 32 5769
PpuMI rG'GwC_Cy
Cuts at: 1170 1170
Size: 5801
PstI C_TGCA'G
Cuts at: 459 777 2848 3533 459
Size: 318 2071 685 2727
Fragments arranged by size:
2727 2071 685 318
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5801
PvuII CAG'CTG
Cuts at: 460 1329 1846 3226 3586 460
Size: 869 517 1380 360 2675
Fragments arranged by size:
2675 1380 869 517 360
RsrII CG'GwC_CG
Cuts at: 3996 3996
Size: 5801
SacI G_AGCT'C
Cuts at: 467 2344 2815 467
Size: 1877 471 3453
Fragments arranged by size:
3453 1877 471
SalI G'TCGA_C
Cuts at: 2838 2838
Size: 5801
SapI GCTCTTCn'nnn_
Cuts at: 1457 2702 3824 4034 1457
Size: 1245 1122 210 3224
Fragments arranged by size:
3224 1245 1122 210
SbfI CC_TGCA'GG
Cuts at: 2848 2848
Size: 5801
ScaI AGT'ACT
Cuts at: 5318 5318
Size: 5801
SfcI C'TryA_G
Cuts at: 33 174 455 773 1141 1267 1477 1676 1841
Size: 141 281 318 368 126 210 199 165
Cuts at: 1841 2844 3529 4497 4688 33
Size: 1003 685 968 191 1146
Fragments arranged by size:
1146 1003 968 685 368 318 281 210
199 191 165 141 126
SfoI GGC'GCC
Cuts at: 3233 3481 3233
Size: 248 5553
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5801
SmaI CCC'GGG
Cuts at: 2823 2823
Size: 5801
SmlI C'TyrA_G
Cuts at: 1174 1342 1920 2956 3871 4585 4847 1174
Size: 168 578 1036 915 714 262 2128
Fragments arranged by size:
2128 1036 915 714 578 262 168
SpeI A'CTAG_T
Cuts at: 67 1408 67
Size: 1341 4460
SphI G_CATG'C
Cuts at: 2854 3354 2854
Size: 500 5301
SspI AAT'ATT
Cuts at: 512 860 2573 512
Size: 348 1713 3740
Fragments arranged by size:
3740 1713 348
StyI C'CwwG_G
Cuts at: 107 469 600 731 956 2935 3289 107
Size: 362 131 131 225 1979 354 2619
Fragments arranged by size:
2619 1979 362 354 225 131 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2379 3384 3517 3976 4185 2379
Size: 1005 133 459 209 3995
Fragments arranged by size:
3995 1005 459 209 133
TatI w'GTAC_w
Cuts at: 5316 5316
Size: 5801
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5318 5350 5318
Size: 32 5769
Tth111I GACn'n_nGTC
Cuts at: 3598 3598
Size: 5801
XbaI T'CTAG_A
Cuts at: 2832 2832
Size: 5801
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2484 3296 2484
Size: 812 4989
XmaI C'CCGG_G
Cuts at: 2821 2821
Size: 5801
XmnI GAAnn'nnTTC
Cuts at: 1028 1538 1028
Size: 510 5291
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BsiWI BsmBI BspEI BsrGI BssHII BstEII Bsu36I CspCI
DraIII FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI