(Circular) (Six-base) MAPSORT of: pF1KB3218.seq Check: 6610 from: 1 to: 5801 pF1KB3218 5801 bp With 182 enzymes: SgfI * June 20, 2008 10:22 .. AccI GT'mk_AC Cuts at: 683 1476 1916 2839 683 Size: 793 440 923 3645 Fragments arranged by size: 3645 923 793 440 Acc65I G'GTAC_C Cuts at: 2817 2817 Size: 5801 AclI AA'CG_TT Cuts at: 2167 5641 2167 Size: 3474 2327 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 506 1525 2067 2271 2628 2681 3626 4058 4411 Size: 1019 542 204 357 53 945 432 353 Cuts at: 4411 5073 506 Size: 662 1234 Fragments arranged by size: 1234 1019 945 662 542 432 357 353 204 53 AflII C'TTAA_G Cuts at: 1174 1174 Size: 5801 AflIII A'CryG_T Cuts at: 1731 3153 4953 5234 1731 Size: 1422 1800 281 2298 Fragments arranged by size: 2298 1800 1422 281 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 2735 2735 Size: 5801 AlwNI CAG_nnn'CTG Cuts at: 3106 4544 3106 Size: 1438 4363 ApaLI G'TGCA_C Cuts at: 689 4639 689 Size: 3950 1851 ApoI r'AATT_y Cuts at: 322 2805 5154 5410 322 Size: 2483 2349 256 713 Fragments arranged by size: 2483 2349 713 256 AseI AT'TA_AT Cuts at: 20 20 Size: 5801 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5801 AvaI C'yCGr_G Cuts at: 2821 2821 Size: 5801 BamHI G'GATC_C Cuts at: 2826 2826 Size: 5801 BanI G'GyrC_C Cuts at: 735 2817 3231 3479 3514 735 Size: 2082 414 248 35 3022 Fragments arranged by size: 3022 2082 414 248 35 BanII G_rGCy'C Cuts at: 467 1766 2344 2815 3845 467 Size: 1299 578 471 1030 2423 Fragments arranged by size: 2423 1299 1030 578 471 BbeI G_GCGC'C Cuts at: 3235 3483 3235 Size: 248 5553 BbsI GAAGACnn'nnnn_ Cuts at: 2753 2753 Size: 5801 BbvCI CC'TCA_GC Cuts at: 1367 1693 1367 Size: 326 5475 BciVI GTATCCnnnnn_n' Cuts at: 316 777 3699 4755 5801 316 Size: 461 2922 1056 1046 316 Fragments arranged by size: 2922 1056 1046 461 316 BclI T'GATC_A Cuts at: 3321 3321 Size: 5801 BglII A'GATC_T Cuts at: 1424 3316 5353 1424 Size: 1892 2037 1872 Fragments arranged by size: 2037 1892 1872 BlpI GC'TnA_GC Cuts at: 461 1468 2913 461 Size: 1007 1445 3349 Fragments arranged by size: 3349 1445 1007 Bme1580I G_kGCm'C Cuts at: 357 693 3426 3519 4643 357 Size: 336 2733 93 1124 1515 Fragments arranged by size: 2733 1515 1124 336 93 BmrI ACTGGGnnnn_n' Cuts at: 523 3013 3094 3429 5573 523 Size: 2490 81 335 2144 751 Fragments arranged by size: 2490 2144 751 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4168 4168 Size: 5801 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 710 715 1351 1766 710 Size: 5 636 415 4745 Fragments arranged by size: 4745 636 415 5 Bpu10I CC'TnA_GC Cuts at: 1367 1693 1367 Size: 326 5475 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1327 1905 2977 3856 4570 4868 1327 Size: 578 1072 879 714 298 2260 Fragments arranged by size: 2260 1072 879 714 578 298 BsaI GGTCTCn'nnnn_ Cuts at: 5505 5505 Size: 5801 BsaAI yAC'GTr Cuts at: 3154 3154 Size: 5801 BsaBI GATnn'nnATC Cuts at: 6 3315 5352 6 Size: 3309 2037 455 Fragments arranged by size: 3309 2037 455 BsaHI Gr'CG_yC Cuts at: 3232 3480 4182 5678 3232 Size: 248 702 1496 3355 Fragments arranged by size: 3355 1496 702 248 BsaWI w'CCGG_w Cuts at: 2990 3511 4189 4600 4747 5021 2990 Size: 521 678 411 147 274 3770 Fragments arranged by size: 3770 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1280 1310 1280 Size: 30 5771 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 995 1348 1384 1516 995 Size: 353 36 132 5280 Fragments arranged by size: 5280 353 132 36 BseYI C'CCAG_C Cuts at: 122 1325 2412 3226 3984 4649 5057 122 Size: 1203 1087 814 758 665 408 866 Fragments arranged by size: 1203 1087 866 814 758 665 408 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1662 2153 1662 Size: 491 5310 BsiEI CG_ry'CG Cuts at: 104 3343 3389 4619 104 Size: 3239 46 1230 1286 Fragments arranged by size: 3239 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 467 693 2344 2815 3593 3783 4643 467 Size: 226 1651 471 778 190 860 1625 Fragments arranged by size: 1651 1625 860 778 471 226 190 BsmI GAATG_Cn' Cuts at: 647 1658 647 Size: 1011 4790 Bsp1286I G_dGCh'C Cuts at: 357 467 693 1766 2344 2815 3426 3519 3593 Size: 110 226 1073 578 471 611 93 74 Cuts at: 3593 3783 3845 4643 357 Size: 190 62 798 1515 Fragments arranged by size: 1515 1073 798 611 578 471 226 190 110 93 74 62 BspHI T'CATG_A Cuts at: 417 4233 417 Size: 3816 1985 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1275 2851 3065 3367 3748 1275 Size: 1576 214 302 381 3328 Fragments arranged by size: 3328 1576 381 302 214 BsrBI CCG'CTC Cuts at: 50 4093 4147 5631 5796 50 Size: 4043 54 1484 165 55 Fragments arranged by size: 4043 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 3713 3713 Size: 5801 BsrFI r'CCGG_y Cuts at: 3799 3980 3799 Size: 181 5620 BssSI C'ACGA_G Cuts at: 4072 4780 4072 Size: 708 5093 BstAPI GCAn_nnn'nTGC Cuts at: 3178 3178 Size: 5801 BstBI TT'CG_AA Cuts at: 4162 4162 Size: 5801 BstXI CCAn_nnnn'nTGG Cuts at: 678 678 Size: 5801 BstYI r'GATC_y Cuts at: 1 1221 1424 1902 2058 2316 2473 2562 2826 Size: 1220 203 478 156 258 157 89 264 Cuts at: 2826 3293 3316 3651 3897 4203 4215 4301 5353 Size: 467 23 335 246 306 12 86 1052 Cuts at: 5353 1 Size: 449 Fragments arranged by size: 1220 1052 478 467 449 335 306 264 258 246 203 157 156 89 86 23 12 BstZ17I GTA'TAC Cuts at: 684 684 Size: 5801 BtgI C'CryG_G Cuts at: 107 469 107 Size: 362 5439 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3751 3932 4095 3751 Size: 181 163 5457 Fragments arranged by size: 5457 181 163 BtsI GCAGTG_nn' Cuts at: 1713 1995 2416 3044 1713 Size: 282 421 628 4470 Fragments arranged by size: 4470 628 421 282 ClaI AT'CG_AT Cuts at: 2024 2024 Size: 5801 DraI TTT'AAA Cuts at: 2508 2800 2508 Size: 292 5509 DrdI GACnn_nn'nnGTC Cuts at: 3507 4851 3507 Size: 1344 4457 EaeI y'GGCC_r Cuts at: 3386 3560 3951 3978 5323 3386 Size: 174 391 27 1345 3864 Fragments arranged by size: 3864 1345 391 174 27 EagI C'GGCC_G Cuts at: 3386 3386 Size: 5801 EarI CTCTTCn'nnn_ Cuts at: 600 1392 1457 1544 2702 3824 4034 600 Size: 792 65 87 1158 1122 210 2367 Fragments arranged by size: 2367 1158 1122 792 210 87 65 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 4009 4751 4897 5611 4009 Size: 742 146 714 4199 Fragments arranged by size: 4199 742 714 146 EcoICRI GAG'CTC Cuts at: 465 2342 2813 465 Size: 1877 471 3453 Fragments arranged by size: 3453 1877 471 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 506 710 715 1351 1525 1766 2067 2271 2628 Size: 204 5 636 174 241 301 204 357 Cuts at: 2628 2681 3626 4058 4411 5073 506 Size: 53 945 432 353 662 1234 Fragments arranged by size: 1234 945 662 636 432 357 353 301 241 204 204 174 53 5 EcoNI CCTnn'n_nnAGG Cuts at: 2048 2534 2048 Size: 486 5315 EcoO109I rG'GnC_Cy Cuts at: 1170 2940 1170 Size: 1770 4031 EcoRI G'AATT_C Cuts at: 322 2805 322 Size: 2483 3318 EcoRV GAT'ATC Cuts at: 597 2786 597 Size: 2189 3612 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3820 3820 Size: 5801 FspI TGC'GCA Cuts at: 3582 3582 Size: 5801 HaeII r_GCGC'y Cuts at: 3235 3483 4713 5484 3235 Size: 248 1230 771 3552 Fragments arranged by size: 3552 1230 771 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 833 865 1374 1406 2371 2403 2421 2453 3643 Size: 32 509 32 965 32 18 32 1190 Cuts at: 3643 3673 3675 3705 833 Size: 30 2 30 2929 Fragments arranged by size: 2929 1190 965 509 32 32 32 32 30 30 18 2 HincII GTy'rAC Cuts at: 1137 2307 2840 1137 Size: 1170 533 4098 Fragments arranged by size: 4098 1170 533 HindIII A'AGCT_T Cuts at: 934 934 Size: 5801 KasI G'GCGC_C Cuts at: 3231 3479 3231 Size: 248 5553 KpnI G_GTAC'C Cuts at: 2821 2821 Size: 5801 MluI A'CGCG_T Cuts at: 5234 5234 Size: 5801 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1511 2464 4560 4744 1511 Size: 953 2096 184 2568 Fragments arranged by size: 2568 2096 953 184 MscI TGG'CCA Cuts at: 3562 3562 Size: 5801 MslI CAynn'nnrTG Cuts at: 3790 3917 3790 Size: 127 5674 MspA1I CmG'CkG Cuts at: 460 1329 1846 2912 3226 3586 4370 4615 460 Size: 869 517 1066 314 360 784 245 1646 Fragments arranged by size: 1646 1066 869 784 517 360 314 245 NaeI GCC'GGC Cuts at: 3982 3982 Size: 5801 NarI GG'CG_CC Cuts at: 3232 3480 3232 Size: 248 5553 NcoI C'CATG_G Cuts at: 107 469 107 Size: 362 5439 NgoMIV G'CCGG_C Cuts at: 3980 3980 Size: 5801 NspI r_CATG'y Cuts at: 353 1735 2854 3354 4957 353 Size: 1382 1119 500 1603 1197 Fragments arranged by size: 1603 1382 1197 1119 500 PciI A'CATG_T Cuts at: 1731 4953 1731 Size: 3222 2579 PflMI CCAn_nnn'nTGG Cuts at: 535 2035 535 Size: 1500 4301 PmeI GTTT'AAAC Cuts at: 2800 2800 Size: 5801 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4247 4279 4247 Size: 32 5769 PpuMI rG'GwC_Cy Cuts at: 1170 1170 Size: 5801 PstI C_TGCA'G Cuts at: 459 777 2848 3533 459 Size: 318 2071 685 2727 Fragments arranged by size: 2727 2071 685 318 PvuI CG_AT'CG Cuts at: 104 104 Size: 5801 PvuII CAG'CTG Cuts at: 460 1329 1846 3226 3586 460 Size: 869 517 1380 360 2675 Fragments arranged by size: 2675 1380 869 517 360 RsrII CG'GwC_CG Cuts at: 3996 3996 Size: 5801 SacI G_AGCT'C Cuts at: 467 2344 2815 467 Size: 1877 471 3453 Fragments arranged by size: 3453 1877 471 SalI G'TCGA_C Cuts at: 2838 2838 Size: 5801 SapI GCTCTTCn'nnn_ Cuts at: 1457 2702 3824 4034 1457 Size: 1245 1122 210 3224 Fragments arranged by size: 3224 1245 1122 210 SbfI CC_TGCA'GG Cuts at: 2848 2848 Size: 5801 ScaI AGT'ACT Cuts at: 5318 5318 Size: 5801 SfcI C'TryA_G Cuts at: 33 174 455 773 1141 1267 1477 1676 1841 Size: 141 281 318 368 126 210 199 165 Cuts at: 1841 2844 3529 4497 4688 33 Size: 1003 685 968 191 1146 Fragments arranged by size: 1146 1003 968 685 368 318 281 210 199 191 165 141 126 SfoI GGC'GCC Cuts at: 3233 3481 3233 Size: 248 5553 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5801 SmaI CCC'GGG Cuts at: 2823 2823 Size: 5801 SmlI C'TyrA_G Cuts at: 1174 1342 1920 2956 3871 4585 4847 1174 Size: 168 578 1036 915 714 262 2128 Fragments arranged by size: 2128 1036 915 714 578 262 168 SpeI A'CTAG_T Cuts at: 67 1408 67 Size: 1341 4460 SphI G_CATG'C Cuts at: 2854 3354 2854 Size: 500 5301 SspI AAT'ATT Cuts at: 512 860 2573 512 Size: 348 1713 3740 Fragments arranged by size: 3740 1713 348 StyI C'CwwG_G Cuts at: 107 469 600 731 956 2935 3289 107 Size: 362 131 131 225 1979 354 2619 Fragments arranged by size: 2619 1979 362 354 225 131 131 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2379 3384 3517 3976 4185 2379 Size: 1005 133 459 209 3995 Fragments arranged by size: 3995 1005 459 209 133 TatI w'GTAC_w Cuts at: 5316 5316 Size: 5801 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5318 5350 5318 Size: 32 5769 Tth111I GACn'n_nGTC Cuts at: 3598 3598 Size: 5801 XbaI T'CTAG_A Cuts at: 2832 2832 Size: 5801 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2484 3296 2484 Size: 812 4989 XmaI C'CCGG_G Cuts at: 2821 2821 Size: 5801 XmnI GAAnn'nnTTC Cuts at: 1028 1538 1028 Size: 510 5291 Enzymes that do cut: AccI Acc65I AclI AcuI AflII AflIII AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AgeI AhdI AleI AloI ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsiWI BsmBI BspEI BsrGI BssHII BstEII Bsu36I CspCI DraIII FseI FspAI HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI