Restriction map for
pF1KB3219
(Circular) (Six-base) MAPSORT of: pF1KB3219.seq Check: 4125 from: 1 to: 5120
pF1KB3219 5120 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1616 1616
Size: 5120
AccI GT'mk_AC
Cuts at: 1673 2158 1673
Size: 485 4635
Acc65I G'GTAC_C
Cuts at: 1993 2136 1993
Size: 143 4977
AclI AA'CG_TT
Cuts at: 4960 4960
Size: 5120
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1429 1678 2945 3377 3730 4392 1429
Size: 249 1267 432 353 662 2157
Fragments arranged by size:
2157 1267 662 432 353 249
AfeI AGC'GCT
Cuts at: 1330 1330
Size: 5120
AflIII A'CryG_T
Cuts at: 560 2472 4272 4553 560
Size: 1912 1800 281 1127
Fragments arranged by size:
1912 1800 1127 281
AgeI A'CCGG_T
Cuts at: 293 293
Size: 5120
AleI CACnn'nnGTG
Cuts at: 124 1753 124
Size: 1629 3491
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2048 2048
Size: 5120
AlwNI CAG_nnn'CTG
Cuts at: 171 711 1230 2425 3863 171
Size: 540 519 1195 1438 1428
Fragments arranged by size:
1438 1428 1195 540 519
ApaI G_GGCC'C
Cuts at: 1062 1542 1923 2046 1062
Size: 480 381 123 4136
Fragments arranged by size:
4136 480 381 123
ApaLI G'TGCA_C
Cuts at: 328 1635 3958 328
Size: 1307 2323 1490
Fragments arranged by size:
2323 1490 1307
ApoI r'AATT_y
Cuts at: 2124 4473 4729 2124
Size: 2349 256 2515
Fragments arranged by size:
2515 2349 256
AscI GG'CGCG_CC
Cuts at: 356 356
Size: 5120
AseI AT'TA_AT
Cuts at: 20 20
Size: 5120
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5120
AvaI C'yCGr_G
Cuts at: 1314 2039 2140 1314
Size: 725 101 4294
Fragments arranged by size:
4294 725 101
BamHI G'GATC_C
Cuts at: 2145 2145
Size: 5120
BanI G'GyrC_C
Cuts at: 625 1297 1474 1993 2136 2550 2798 2833 625
Size: 672 177 519 143 414 248 35 2912
Fragments arranged by size:
2912 672 519 414 248 177 143 35
BanII G_rGCy'C
Cuts at: 918 1062 1130 1393 1542 1562 1923 2046 2134
Size: 144 68 263 149 20 361 123 88
Cuts at: 2134 3164 918
Size: 1030 2874
Fragments arranged by size:
2874 1030 361 263 149 144 123 88
68 20
BbeI G_GCGC'C
Cuts at: 629 2554 2802 629
Size: 1925 248 2947
Fragments arranged by size:
2947 1925 248
BbsI GAAGACnn'nnnn_
Cuts at: 881 1957 881
Size: 1076 4044
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1464 1498 1464
Size: 34 5086
BciVI GTATCCnnnnn_n'
Cuts at: 3018 4074 5120 3018
Size: 1056 1046 3018
Fragments arranged by size:
3018 1056 1046
BclI T'GATC_A
Cuts at: 1196 2640 1196
Size: 1444 3676
BglII A'GATC_T
Cuts at: 1047 2088 2635 4672 1047
Size: 1041 547 2037 1495
Fragments arranged by size:
2037 1495 1041 547
BlpI GC'TnA_GC
Cuts at: 175 854 2232 175
Size: 679 1378 3063
Fragments arranged by size:
3063 1378 679
Bme1580I G_kGCm'C
Cuts at: 332 1062 1477 1542 1639 1923 2046 2745 2838
Size: 730 415 65 97 284 123 699 93
Cuts at: 2838 3962 332
Size: 1124 1490
Fragments arranged by size:
1490 1124 730 699 415 284 123 97
93 65
BmgBI CAC'GTC
Cuts at: 273 399 273
Size: 126 4994
BmrI ACTGGGnnnn_n'
Cuts at: 1569 2332 2413 2748 4892 1569
Size: 763 81 335 2144 1797
Fragments arranged by size:
2144 1797 763 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3487 3487
Size: 5120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 514 556 637 715 1092 1207 1399 1642
Size: 366 42 81 78 377 115 192 243
Cuts at: 1642 2113 148
Size: 471 3155
Fragments arranged by size:
3155 471 377 366 243 192 115 81
78 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1669 2296 3175 3889 4187 1669
Size: 627 879 714 298 2602
Fragments arranged by size:
2602 879 714 627 298
BsaI GGTCTCn'nnnn_
Cuts at: 337 4824 337
Size: 4487 633
BsaAI yAC'GTr
Cuts at: 563 1028 1440 1794 2016 2473 563
Size: 465 412 354 222 457 3210
Fragments arranged by size:
3210 465 457 412 354 222
BsaBI GATnn'nnATC
Cuts at: 6 2634 4671 6
Size: 2628 2037 455
Fragments arranged by size:
2628 2037 455
BsaHI Gr'CG_yC
Cuts at: 626 1931 2551 2799 3501 4997 626
Size: 1305 620 248 702 1496 749
Fragments arranged by size:
1496 1305 749 702 620 248
BsaWI w'CCGG_w
Cuts at: 293 1122 2309 2830 3508 3919 4066 4340 293
Size: 829 1187 521 678 411 147 274 1073
Fragments arranged by size:
1187 1073 829 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 921 1195 921
Size: 274 4846
BseYI C'CCAG_C
Cuts at: 714 1017 1642 2545 3303 3968 4376 714
Size: 303 625 903 758 665 408 1458
Fragments arranged by size:
1458 903 758 665 625 408 303
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1708 1774 2095 1708
Size: 66 321 4733
Fragments arranged by size:
4733 321 66
BsiEI CG_ry'CG
Cuts at: 104 183 2662 2708 3938 104
Size: 79 2479 46 1230 1286
Fragments arranged by size:
2479 1286 1230 79 46
BsiHKAI G_wGCw'C
Cuts at: 332 524 1639 1655 2134 2912 3102 3962 332
Size: 192 1115 16 479 778 190 860 1490
Fragments arranged by size:
1490 1115 860 778 479 192 190 16
BsmI GAATG_Cn'
Cuts at: 1905 1905
Size: 5120
Bsp1286I G_dGCh'C
Cuts at: 332 524 918 1062 1130 1393 1477 1542 1562
Size: 192 394 144 68 263 84 65 20
Cuts at: 1562 1639 1655 1923 2046 2134 2745 2838 2912
Size: 77 16 268 123 88 611 93 74
Cuts at: 2912 3102 3164 3962 332
Size: 190 62 798 1490
Fragments arranged by size:
1490 798 611 394 268 263 192 190
144 123 93 88 84 77 74 68
65 62 20 16
BspHI T'CATG_A
Cuts at: 569 1895 3552 569
Size: 1326 1657 2137
Fragments arranged by size:
2137 1657 1326
BspMI ACCTGCnnnn'nnnn_
Cuts at: 620 825 1121 1616 1625 2170 2384 2686 3067
Size: 205 296 495 9 545 214 302 381
Cuts at: 3067 620
Size: 2673
Fragments arranged by size:
2673 545 495 381 302 296 214 205
9
BsrBI CCG'CTC
Cuts at: 50 3412 3466 4950 5115 50
Size: 3362 54 1484 165 55
Fragments arranged by size:
3362 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3032 3032
Size: 5120
BsrFI r'CCGG_y
Cuts at: 223 293 316 385 548 638 3118 3299 223
Size: 70 23 69 163 90 2480 181 2044
Fragments arranged by size:
2480 2044 181 163 90 70 69 23
BsrGI T'GTAC_A
Cuts at: 128 128
Size: 5120
BssHII G'CGCG_C
Cuts at: 356 1691 356
Size: 1335 3785
BssSI C'ACGA_G
Cuts at: 576 3391 4099 576
Size: 2815 708 1597
Fragments arranged by size:
2815 1597 708
BstAPI GCAn_nnn'nTGC
Cuts at: 711 2497 711
Size: 1786 3334
BstBI TT'CG_AA
Cuts at: 3481 3481
Size: 5120
BstXI CCAn_nnnn'nTGG
Cuts at: 798 1590 1755 798
Size: 792 165 4163
Fragments arranged by size:
4163 792 165
BstYI r'GATC_y
Cuts at: 1 698 1047 2060 2088 2145 2612 2635 2970
Size: 697 349 1013 28 57 467 23 335
Cuts at: 2970 3216 3522 3534 3620 4672 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 1013 697 467 449 349 335 306
246 86 57 28 23 12
Bsu36I CC'TnA_GG
Cuts at: 908 908
Size: 5120
BtgI C'CryG_G
Cuts at: 107 420 998 1082 1769 107
Size: 313 578 84 687 3458
Fragments arranged by size:
3458 687 578 313 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 231 3070 3251 3414 231
Size: 2839 181 163 1937
Fragments arranged by size:
2839 1937 181 163
BtsI GCAGTG_nn'
Cuts at: 2032 2363 2032
Size: 331 4789
DraI TTT'AAA
Cuts at: 2119 2119
Size: 5120
DrdI GACnn_nn'nnGTC
Cuts at: 2826 4170 2826
Size: 1344 3776
EaeI y'GGCC_r
Cuts at: 180 221 261 387 486 542 555 770 825
Size: 41 40 126 99 56 13 215 55
Cuts at: 825 1592 1889 1985 2705 2879 3270 3297 4642
Size: 767 297 96 720 174 391 27 1345
Cuts at: 4642 180
Size: 658
Fragments arranged by size:
1345 767 720 658 391 297 215 174
126 99 96 56 55 41 40 27
13
EagI C'GGCC_G
Cuts at: 180 2705 180
Size: 2525 2595
EarI CTCTTCn'nnn_
Cuts at: 1435 3143 3353 1435
Size: 1708 210 3202
Fragments arranged by size:
3202 1708 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3328 4070 4216 4930 3328
Size: 742 146 714 3518
Fragments arranged by size:
3518 742 714 146
EcoICRI GAG'CTC
Cuts at: 2132 2132
Size: 5120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 514 556 637 715 1092 1207 1399 1429
Size: 366 42 81 78 377 115 192 30
Cuts at: 1429 1642 1678 2113 2945 3377 3730 4392 148
Size: 213 36 435 832 432 353 662 876
Fragments arranged by size:
876 832 662 435 432 377 366 353
213 192 115 81 78 42 36 30
EcoNI CCTnn'n_nnAGG
Cuts at: 811 811
Size: 5120
EcoO109I rG'GnC_Cy
Cuts at: 425 1058 1059 1227 1539 1919 2043 2259 425
Size: 633 1 168 312 380 124 216 3286
Fragments arranged by size:
3286 633 380 312 216 168 124 1
EcoRI G'AATT_C
Cuts at: 2124 2124
Size: 5120
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1728 3139 1728
Size: 1411 3709
FspI TGC'GCA
Cuts at: 902 1219 2901 902
Size: 317 1682 3121
Fragments arranged by size:
3121 1682 317
FspAI rTGC'GCAy
Cuts at: 1219 1219
Size: 5120
HaeII r_GCGC'y
Cuts at: 629 650 1195 1332 1591 2554 2802 4032 4803
Size: 21 545 137 259 963 248 1230 771
Cuts at: 4803 629
Size: 946
Fragments arranged by size:
1230 963 946 771 545 259 248 137
21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2962 2992 2994 3024 2962
Size: 30 2 30 5058
Fragments arranged by size:
5058 30 30 2
HincII GTy'rAC
Cuts at: 1170 2159 1170
Size: 989 4131
KasI G'GCGC_C
Cuts at: 625 2550 2798 625
Size: 1925 248 2947
Fragments arranged by size:
2947 1925 248
KpnI G_GTAC'C
Cuts at: 1997 2140 1997
Size: 143 4977
MluI A'CGCG_T
Cuts at: 4553 4553
Size: 5120
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3879 4063 3879
Size: 184 4936
MscI TGG'CCA
Cuts at: 544 1594 1987 2881 544
Size: 1050 393 894 2783
Fragments arranged by size:
2783 1050 894 393
MslI CAynn'nnrTG
Cuts at: 124 335 1732 1753 3109 3236 124
Size: 211 1397 21 1356 127 2008
Fragments arranged by size:
2008 1397 1356 211 127 21
MspA1I CmG'CkG
Cuts at: 159 179 366 422 708 762 1137 1548 2231
Size: 20 187 56 286 54 375 411 683
Cuts at: 2231 2545 2905 3689 3934 159
Size: 314 360 784 245 1345
Fragments arranged by size:
1345 784 683 411 375 360 314 286
245 187 56 54 20
NaeI GCC'GGC
Cuts at: 225 387 3301 225
Size: 162 2914 2044
Fragments arranged by size:
2914 2044 162
NarI GG'CG_CC
Cuts at: 626 2551 2799 626
Size: 1925 248 2947
Fragments arranged by size:
2947 1925 248
NcoI C'CATG_G
Cuts at: 107 1082 107
Size: 975 4145
NgoMIV G'CCGG_C
Cuts at: 223 385 3299 223
Size: 162 2914 2044
Fragments arranged by size:
2914 2044 162
NspI r_CATG'y
Cuts at: 1224 1365 2173 2673 4276 1224
Size: 141 808 500 1603 2068
Fragments arranged by size:
2068 1603 808 500 141
PasI CC'CwG_GG
Cuts at: 1553 1697 1553
Size: 144 4976
PciI A'CATG_T
Cuts at: 4272 4272
Size: 5120
PfoI T'CCnGG_A
Cuts at: 812 1238 1523 2081 812
Size: 426 285 558 3851
Fragments arranged by size:
3851 558 426 285
PmeI GTTT'AAAC
Cuts at: 2119 2119
Size: 5120
PmlI CAC'GTG
Cuts at: 563 1794 563
Size: 1231 3889
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3566 3598 3566
Size: 32 5088
PspOMI G'GGCC_C
Cuts at: 1058 1538 1919 2042 1058
Size: 480 381 123 4136
Fragments arranged by size:
4136 480 381 123
PstI C_TGCA'G
Cuts at: 158 707 928 1547 2027 2167 2852 158
Size: 549 221 619 480 140 685 2426
Fragments arranged by size:
2426 685 619 549 480 221 140
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5120
PvuII CAG'CTG
Cuts at: 159 708 1137 1548 2545 2905 159
Size: 549 429 411 997 360 2374
Fragments arranged by size:
2374 997 549 429 411 360
RsrII CG'GwC_CG
Cuts at: 3315 3315
Size: 5120
SacI G_AGCT'C
Cuts at: 2134 2134
Size: 5120
SacII CC_GC'GG
Cuts at: 423 423
Size: 5120
SalI G'TCGA_C
Cuts at: 2157 2157
Size: 5120
SapI GCTCTTCn'nnn_
Cuts at: 3143 3353 3143
Size: 210 4910
SbfI CC_TGCA'GG
Cuts at: 2167 2167
Size: 5120
ScaI AGT'ACT
Cuts at: 4637 4637
Size: 5120
SexAI A'CCwGG_T
Cuts at: 284 509 284
Size: 225 4895
SfcI C'TryA_G
Cuts at: 33 154 703 924 1543 2023 2163 2848 3816
Size: 121 549 221 619 480 140 685 968
Cuts at: 3816 4007 33
Size: 191 1146
Fragments arranged by size:
1146 968 685 619 549 480 221 191
140 121
SfoI GGC'GCC
Cuts at: 627 2552 2800 627
Size: 1925 248 2947
Fragments arranged by size:
2947 1925 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5120
SmaI CCC'GGG
Cuts at: 2041 2142 2041
Size: 101 5019
SmlI C'TyrA_G
Cuts at: 1684 2275 3190 3904 4166 1684
Size: 591 915 714 262 2638
Fragments arranged by size:
2638 915 714 591 262
SnaBI TAC'GTA
Cuts at: 1028 1028
Size: 5120
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5120
SphI G_CATG'C
Cuts at: 1224 2173 2673 1224
Size: 949 500 3671
Fragments arranged by size:
3671 949 500
SrfI GCCC'GGGC
Cuts at: 2041 2041
Size: 5120
StyI C'CwwG_G
Cuts at: 107 650 1007 1082 1583 2254 2608 107
Size: 543 357 75 501 671 354 2619
Fragments arranged by size:
2619 671 543 501 357 354 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1243 1492 2703 2836 3295 3504 1243
Size: 249 1211 133 459 209 2859
Fragments arranged by size:
2859 1211 459 249 209 133
TatI w'GTAC_w
Cuts at: 128 4635 128
Size: 4507 613
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1124 1156 4637 4669 1124
Size: 32 3481 32 1575
Fragments arranged by size:
3481 1575 32 32
Tth111I GACn'n_nGTC
Cuts at: 2917 2917
Size: 5120
XbaI T'CTAG_A
Cuts at: 2151 2151
Size: 5120
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1182 1467 2615 1182
Size: 285 1148 3687
Fragments arranged by size:
3687 1148 285
XmaI C'CCGG_G
Cuts at: 2039 2140 2039
Size: 101 5019
XmnI GAAnn'nnTTC
Cuts at: 1711 1711
Size: 5120
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PfoI PmeI PmlI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SrfI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AloI AvrII BaeI BbvCI BfrBI
BglI BmtI Bpu10I BsaXI BsiWI BsmBI BspEI BstEII
BstZ17I ClaI CspCI DraIII EcoRV FseI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PpuMI PshAI PsiI PspXI PsrI SanDI SfiI SgrAI
SspI StuI SwaI XhoI ZraI