Restriction map for
pF1KB3224
(Circular) (Six-base) MAPSORT of: pF1KB3224.seq Check: 4256 from: 1 to: 5354
pF1KB3224 5354 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 310 2392 310
Size: 2082 3272
Acc65I G'GTAC_C
Cuts at: 2370 2370
Size: 5354
AclI AA'CG_TT
Cuts at: 5194 5194
Size: 5354
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 541 602 631 809 956 1148 1979 2246 3179
Size: 61 29 178 147 192 831 267 933
Cuts at: 3179 3611 3964 4626 541
Size: 432 353 662 1269
Fragments arranged by size:
1269 933 831 662 432 353 267 192
178 147 61 29
AflII C'TTAA_G
Cuts at: 1237 1237
Size: 5354
AflIII A'CryG_T
Cuts at: 1162 2706 4506 4787 1162
Size: 1544 1800 281 1729
Fragments arranged by size:
1800 1729 1544 281
AhdI GACnn_n'nnGTC
Cuts at: 1323 1323
Size: 5354
AleI CACnn'nnGTG
Cuts at: 181 181
Size: 5354
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1112 1112
Size: 5354
AlwNI CAG_nnn'CTG
Cuts at: 206 439 1105 2659 4097 206
Size: 233 666 1554 1438 1463
Fragments arranged by size:
1554 1463 1438 666 233
ApaLI G'TGCA_C
Cuts at: 4192 4192
Size: 5354
ApoI r'AATT_y
Cuts at: 2358 4707 4963 2358
Size: 2349 256 2749
Fragments arranged by size:
2749 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5354
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5354
AvaI C'yCGr_G
Cuts at: 2374 2374
Size: 5354
BamHI G'GATC_C
Cuts at: 2115 2379 2115
Size: 264 5090
BanI G'GyrC_C
Cuts at: 2370 2784 3032 3067 2370
Size: 414 248 35 4657
Fragments arranged by size:
4657 414 248 35
BanII G_rGCy'C
Cuts at: 964 2368 3398 964
Size: 1404 1030 2920
Fragments arranged by size:
2920 1404 1030
BbeI G_GCGC'C
Cuts at: 2788 3036 2788
Size: 248 5106
BbvCI CC'TCA_GC
Cuts at: 1370 1370
Size: 5354
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 806 840 90
Size: 34 682 34 4604
Fragments arranged by size:
4604 682 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1152 1774 3252 4308 5354 1152
Size: 622 1478 1056 1046 1152
Fragments arranged by size:
1478 1152 1056 1046 622
BclI T'GATC_A
Cuts at: 273 1056 2874 273
Size: 783 1818 2753
Fragments arranged by size:
2753 1818 783
BfrBI ATG'CAT
Cuts at: 946 946
Size: 5354
BglI GCCn_nnn'nGGC
Cuts at: 245 245
Size: 5354
BglII A'GATC_T
Cuts at: 2869 4906 2869
Size: 2037 3317
BlpI GC'TnA_GC
Cuts at: 1625 2466 1625
Size: 841 4513
Bme1580I G_kGCm'C
Cuts at: 2979 3072 4196 2979
Size: 93 1124 4137
Fragments arranged by size:
4137 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 335 446 552 1864 2566 2647 2982 5126 335
Size: 111 106 1312 702 81 335 2144 563
Fragments arranged by size:
2144 1312 702 563 335 111 106 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3721 3721
Size: 5354
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 731 916 1631 2125 184
Size: 547 185 715 494 3413
Fragments arranged by size:
3413 715 547 494 185
Bpu10I CC'TnA_GC
Cuts at: 1370 1370
Size: 5354
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2044 2530 3409 4123 4421 2044
Size: 486 879 714 298 2977
Fragments arranged by size:
2977 879 714 486 298
BsaI GGTCTCn'nnnn_
Cuts at: 660 2091 5058 660
Size: 1431 2967 956
Fragments arranged by size:
2967 1431 956
BsaAI yAC'GTr
Cuts at: 2707 2707
Size: 5354
BsaBI GATnn'nnATC
Cuts at: 6 2868 4905 6
Size: 2862 2037 455
Fragments arranged by size:
2862 2037 455
BsaHI Gr'CG_yC
Cuts at: 2785 3033 3735 5231 2785
Size: 248 702 1496 2908
Fragments arranged by size:
2908 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2543 3064 3742 4153 4300 4574 2543
Size: 521 678 411 147 274 3323
Fragments arranged by size:
3323 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 657 687 657
Size: 30 5324
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 596 678 596
Size: 82 5272
BseYI C'CCAG_C
Cuts at: 364 1242 2294 2779 3537 4202 4610 364
Size: 878 1052 485 758 665 408 1108
Fragments arranged by size:
1108 1052 878 758 665 485 408
BsiEI CG_ry'CG
Cuts at: 104 2896 2942 4172 104
Size: 2792 46 1230 1286
Fragments arranged by size:
2792 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2368 3146 3336 4196 2368
Size: 778 190 860 3526
Fragments arranged by size:
3526 860 778 190
BsmI GAATG_Cn'
Cuts at: 829 829
Size: 5354
Bsp1286I G_dGCh'C
Cuts at: 964 2368 2979 3072 3146 3336 3398 4196 964
Size: 1404 611 93 74 190 62 798 2122
Fragments arranged by size:
2122 1404 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 1416 3786 1416
Size: 2370 2984
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1620 2404 2618 2920 3301 1620
Size: 784 214 302 381 3673
Fragments arranged by size:
3673 784 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3646 3700 5184 5349 50
Size: 3596 54 1484 165 55
Fragments arranged by size:
3596 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 964 1436 2046 3266 964
Size: 472 610 1220 3052
Fragments arranged by size:
3052 1220 610 472
BsrFI r'CCGG_y
Cuts at: 1068 3352 3533 1068
Size: 2284 181 2889
Fragments arranged by size:
2889 2284 181
BsrGI T'GTAC_A
Cuts at: 565 1672 565
Size: 1107 4247
BssSI C'ACGA_G
Cuts at: 3625 4333 3625
Size: 708 4646
BstAPI GCAn_nnn'nTGC
Cuts at: 2731 2731
Size: 5354
BstBI TT'CG_AA
Cuts at: 3715 3715
Size: 5354
BstEII G'GTnAC_C
Cuts at: 183 183
Size: 5354
BstXI CCAn_nnnn'nTGG
Cuts at: 1986 1986
Size: 5354
BstYI r'GATC_y
Cuts at: 1 864 2115 2379 2846 2869 3204 3450 3756
Size: 863 1251 264 467 23 335 246 306
Cuts at: 3756 3768 3854 4906 1
Size: 12 86 1052 449
Fragments arranged by size:
1251 1052 863 467 449 335 306 264
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 311 311
Size: 5354
BtgI C'CryG_G
Cuts at: 240 890 240
Size: 650 4704
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3304 3485 3648 3304
Size: 181 163 5010
Fragments arranged by size:
5010 181 163
BtsI GCAGTG_nn'
Cuts at: 255 2084 2597 255
Size: 1829 513 3012
Fragments arranged by size:
3012 1829 513
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2039 2074 2039
Size: 35 5319
DraI TTT'AAA
Cuts at: 353 2353 353
Size: 2000 3354
DraIII CAC_nnn'GTG
Cuts at: 329 454 329
Size: 125 5229
DrdI GACnn_nn'nnGTC
Cuts at: 3060 4404 3060
Size: 1344 4010
EaeI y'GGCC_r
Cuts at: 840 2939 3113 3504 3531 4876 840
Size: 2099 174 391 27 1345 1318
Fragments arranged by size:
2099 1345 1318 391 174 27
EagI C'GGCC_G
Cuts at: 2939 2939
Size: 5354
EarI CTCTTCn'nnn_
Cuts at: 1079 1784 3377 3587 1079
Size: 705 1593 210 2846
Fragments arranged by size:
2846 1593 705 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3562 4304 4450 5164 3562
Size: 742 146 714 3752
Fragments arranged by size:
3752 742 714 146
EcoICRI GAG'CTC
Cuts at: 2366 2366
Size: 5354
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 541 602 631 731 809 916 956 1148
Size: 357 61 29 100 78 107 40 192
Cuts at: 1148 1631 1979 2125 2246 3179 3611 3964 4626
Size: 483 348 146 121 933 432 353 662
Cuts at: 4626 184
Size: 912
Fragments arranged by size:
933 912 662 483 432 357 353 348
192 146 121 107 100 78 61 40
29
EcoO109I rG'GnC_Cy
Cuts at: 2493 2493
Size: 5354
EcoRI G'AATT_C
Cuts at: 2358 2358
Size: 5354
EcoRV GAT'ATC
Cuts at: 1007 1007
Size: 5354
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1314 1783 3373 1314
Size: 469 1590 3295
Fragments arranged by size:
3295 1590 469
FspI TGC'GCA
Cuts at: 1700 3135 1700
Size: 1435 3919
FspAI rTGC'GCAy
Cuts at: 1700 1700
Size: 5354
HaeII r_GCGC'y
Cuts at: 2788 3036 4266 5037 2788
Size: 248 1230 771 3105
Fragments arranged by size:
3105 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 203 235 657 689 1309 1341 1498 1530 1748
Size: 32 422 32 620 32 157 32 218
Cuts at: 1748 1780 3196 3226 3228 3258 203
Size: 32 1416 30 2 30 2299
Fragments arranged by size:
2299 1416 620 422 218 157 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1875 2393 1875
Size: 518 4836
KasI G'GCGC_C
Cuts at: 2784 3032 2784
Size: 248 5106
KpnI G_GTAC'C
Cuts at: 2374 2374
Size: 5354
MluI A'CGCG_T
Cuts at: 4787 4787
Size: 5354
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 353 681 1734 1757 2353 4113 4297 353
Size: 328 1053 23 596 1760 184 1410
Fragments arranged by size:
1760 1410 1053 596 328 184 23
MscI TGG'CCA
Cuts at: 842 3115 842
Size: 2273 3081
MslI CAynn'nnrTG
Cuts at: 181 3343 3470 181
Size: 3162 127 2065
Fragments arranged by size:
3162 2065 127
MspA1I CmG'CkG
Cuts at: 181 206 242 1369 1957 2465 2779 3139 3923
Size: 25 36 1127 588 508 314 360 784
Cuts at: 3923 4168 181
Size: 245 1367
Fragments arranged by size:
1367 1127 784 588 508 360 314 245
36 25
NaeI GCC'GGC
Cuts at: 3535 3535
Size: 5354
NarI GG'CG_CC
Cuts at: 2785 3033 2785
Size: 248 5106
NcoI C'CATG_G
Cuts at: 890 890
Size: 5354
NdeI CA'TA_TG
Cuts at: 1818 1818
Size: 5354
NgoMIV G'CCGG_C
Cuts at: 3533 3533
Size: 5354
NsiI A_TGCA'T
Cuts at: 948 948
Size: 5354
NspI r_CATG'y
Cuts at: 946 1166 1705 1734 2407 2907 4510 946
Size: 220 539 29 673 500 1603 1790
Fragments arranged by size:
1790 1603 673 539 500 220 29
PasI CC'CwG_GG
Cuts at: 1542 1542
Size: 5354
PciI A'CATG_T
Cuts at: 1162 4506 1162
Size: 3344 2010
PflMI CCAn_nnn'nTGG
Cuts at: 759 1930 759
Size: 1171 4183
PfoI T'CCnGG_A
Cuts at: 1486 1486
Size: 5354
PmeI GTTT'AAAC
Cuts at: 2353 2353
Size: 5354
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3800 3832 3800
Size: 32 5322
PstI C_TGCA'G
Cuts at: 2401 3086 2401
Size: 685 4669
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5354
PvuII CAG'CTG
Cuts at: 206 1369 1957 2779 3139 206
Size: 1163 588 822 360 2421
Fragments arranged by size:
2421 1163 822 588 360
RsrII CG'GwC_CG
Cuts at: 3549 3549
Size: 5354
SacI G_AGCT'C
Cuts at: 2368 2368
Size: 5354
SacII CC_GC'GG
Cuts at: 243 243
Size: 5354
SalI G'TCGA_C
Cuts at: 2391 2391
Size: 5354
SapI GCTCTTCn'nnn_
Cuts at: 1079 3377 3587 1079
Size: 2298 210 2846
Fragments arranged by size:
2846 2298 210
SbfI CC_TGCA'GG
Cuts at: 2401 2401
Size: 5354
ScaI AGT'ACT
Cuts at: 379 1399 4871 379
Size: 1020 3472 862
Fragments arranged by size:
3472 1020 862
SfcI C'TryA_G
Cuts at: 33 518 1193 2397 3082 4050 4241 33
Size: 485 675 1204 685 968 191 1146
Fragments arranged by size:
1204 1146 968 685 675 485 191
SfoI GGC'GCC
Cuts at: 2786 3034 2786
Size: 248 5106
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5354
SmaI CCC'GGG
Cuts at: 2376 2376
Size: 5354
SmlI C'TyrA_G
Cuts at: 1237 2023 2509 3424 4138 4400 1237
Size: 786 486 915 714 262 2191
Fragments arranged by size:
2191 915 786 714 486 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5354
SphI G_CATG'C
Cuts at: 946 2407 2907 946
Size: 1461 500 3393
Fragments arranged by size:
3393 1461 500
SspI AAT'ATT
Cuts at: 2272 2272
Size: 5354
StyI C'CwwG_G
Cuts at: 854 890 1843 2257 2488 2842 854
Size: 36 953 414 231 354 3366
Fragments arranged by size:
3366 953 414 354 231 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 317 465 1555 1814 1846 2937 3070 3529 3738
Size: 148 1090 259 32 1091 133 459 209
Cuts at: 3738 317
Size: 1933
Fragments arranged by size:
1933 1091 1090 459 259 209 148 133
32
TatI w'GTAC_w
Cuts at: 266 335 377 565 1397 1672 1793 4869 266
Size: 69 42 188 832 275 121 3076 751
Fragments arranged by size:
3076 832 751 275 188 121 69 42
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1674 1706 4871 4903 1674
Size: 32 3165 32 2125
Fragments arranged by size:
3165 2125 32 32
Tth111I GACn'n_nGTC
Cuts at: 671 3151 671
Size: 2480 2874
XbaI T'CTAG_A
Cuts at: 397 2385 397
Size: 1988 3366
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1027 2849 1027
Size: 1822 3532
XmaI C'CCGG_G
Cuts at: 2374 2374
Size: 5354
XmnI GAAnn'nnTTC
Cuts at: 827 1475 827
Size: 648 4706
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AhdI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AloI ApaI AscI AvrII
BaeI BbsI BmgBI BmtI BsgI BsiWI BsmBI BspEI
BssHII Bsu36I ClaI EcoNI FseI HindIII HpaI MfeI
NheI NotI NruI PacI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI