Restriction map for
pF1KB3226
(Circular) (Six-base) MAPSORT of: pF1KB3226.seq Check: 443 from: 1 to: 4973
pF1KB3226 4973 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 288 1463 1525 288
Size: 1175 62 3736
Fragments arranged by size:
3736 1175 62
AccI GT'mk_AC
Cuts at: 2011 2011
Size: 4973
Acc65I G'GTAC_C
Cuts at: 1989 1989
Size: 4973
AclI AA'CG_TT
Cuts at: 4813 4813
Size: 4973
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 2798 3230 3583 4245 283
Size: 2515 432 353 662 1011
Fragments arranged by size:
2515 1011 662 432 353
AflIII A'CryG_T
Cuts at: 1445 2325 4125 4406 1445
Size: 880 1800 281 2012
Fragments arranged by size:
2012 1800 880 281
AhdI GACnn_n'nnGTC
Cuts at: 336 405 336
Size: 69 4904
AleI CACnn'nnGTG
Cuts at: 1018 1831 1018
Size: 813 4160
AlwNI CAG_nnn'CTG
Cuts at: 215 723 2278 3716 215
Size: 508 1555 1438 1472
Fragments arranged by size:
1555 1472 1438 508
ApaI G_GGCC'C
Cuts at: 1004 1167 1660 1004
Size: 163 493 4317
Fragments arranged by size:
4317 493 163
ApaLI G'TGCA_C
Cuts at: 3811 3811
Size: 4973
ApoI r'AATT_y
Cuts at: 1977 4326 4582 1977
Size: 2349 256 2368
Fragments arranged by size:
2368 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4973
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4973
AvaI C'yCGr_G
Cuts at: 176 693 925 945 972 997 1231 1282 1675
Size: 517 232 20 27 25 234 51 393
Cuts at: 1675 1993 176
Size: 318 3156
Fragments arranged by size:
3156 517 393 318 234 232 51 27
25 20
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1114 1147 1114
Size: 33 4940
BamHI G'GATC_C
Cuts at: 1867 1998 1867
Size: 131 4842
BanI G'GyrC_C
Cuts at: 367 411 602 685 783 871 930 949 1989
Size: 44 191 83 98 88 59 19 1040
Cuts at: 1989 2403 2651 2686 367
Size: 414 248 35 2654
Fragments arranged by size:
2654 1040 414 248 191 98 88 83
59 44 35 19
BanII G_rGCy'C
Cuts at: 655 914 946 1004 1167 1418 1660 1931 1987
Size: 259 32 58 163 251 242 271 56
Cuts at: 1987 3017 655
Size: 1030 2611
Fragments arranged by size:
2611 1030 271 259 251 242 163 58
56 32
BbeI G_GCGC'C
Cuts at: 415 606 689 787 875 934 953 2407 2655
Size: 191 83 98 88 59 19 1454 248
Cuts at: 2655 415
Size: 2733
Fragments arranged by size:
2733 1454 248 191 98 88 83 59
19
BbsI GAAGACnn'nnnn_
Cuts at: 1546 1785 1546
Size: 239 4734
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 334 368 408 442 334
Size: 34 40 34 4865
Fragments arranged by size:
4865 40 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1115 1956 2871 3927 4973 1115
Size: 841 915 1056 1046 1115
Fragments arranged by size:
1115 1056 1046 915 841
BclI T'GATC_A
Cuts at: 2493 2493
Size: 4973
BglI GCCn_nnn'nGGC
Cuts at: 384 763 1430 1439 384
Size: 379 667 9 3918
Fragments arranged by size:
3918 667 379 9
BglII A'GATC_T
Cuts at: 2488 4525 2488
Size: 2037 2936
BlpI GC'TnA_GC
Cuts at: 1925 2085 1925
Size: 160 4813
Bme1580I G_kGCm'C
Cuts at: 320 1004 1167 1232 1513 1660 2598 2691 3815
Size: 684 163 65 281 147 938 93 1124
Cuts at: 3815 320
Size: 1478
Fragments arranged by size:
1478 1124 938 684 281 163 147 93
65
BmgBI CAC'GTC
Cuts at: 1209 1802 1209
Size: 593 4380
BmrI ACTGGGnnnn_n'
Cuts at: 1094 2185 2266 2601 4745 1094
Size: 1091 81 335 2144 1322
Fragments arranged by size:
2144 1322 1091 335 81
BmtI G_CTAG'C
Cuts at: 1904 1904
Size: 4973
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3340 3340
Size: 4973
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 815 842 815
Size: 27 4946
Bpu10I CC'TnA_GC
Cuts at: 1412 1661 1412
Size: 249 4724
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2149 3028 3742 4040 2149
Size: 879 714 298 3082
Fragments arranged by size:
3082 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4677 4677
Size: 4973
BsaAI yAC'GTr
Cuts at: 2326 2326
Size: 4973
BsaBI GATnn'nnATC
Cuts at: 6 2487 4524 6
Size: 2481 2037 455
Fragments arranged by size:
2481 2037 455
BsaHI Gr'CG_yC
Cuts at: 305 412 603 686 784 872 931 950 2404
Size: 107 191 83 98 88 59 19 1454
Cuts at: 2404 2652 3354 4850 305
Size: 248 702 1496 428
Fragments arranged by size:
1496 1454 702 428 248 191 107 98
88 83 59 19
BsaWI w'CCGG_w
Cuts at: 2162 2683 3361 3772 3919 4193 2162
Size: 521 678 411 147 274 2942
Fragments arranged by size:
2942 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 308 853 1004 2398 3156 3821 4229 308
Size: 545 151 1394 758 665 408 1052
Fragments arranged by size:
1394 1052 758 665 545 408 151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1165 1323 1165
Size: 158 4815
BsiEI CG_ry'CG
Cuts at: 104 1139 2515 2561 3791 104
Size: 1035 1376 46 1230 1286
Fragments arranged by size:
1376 1286 1230 1035 46
BsiHKAI G_wGCw'C
Cuts at: 209 1418 1667 1931 1987 2765 2955 3815 209
Size: 1209 249 264 56 778 190 860 1367
Fragments arranged by size:
1367 1209 860 778 264 249 190 56
Bsp1286I G_dGCh'C
Cuts at: 209 320 655 914 946 1004 1167 1232 1418
Size: 111 335 259 32 58 163 65 186
Cuts at: 1418 1513 1660 1667 1931 1987 2598 2691 2765
Size: 95 147 7 264 56 611 93 74
Cuts at: 2765 2955 3017 3815 209
Size: 190 62 798 1367
Fragments arranged by size:
1367 798 611 335 264 259 190 186
163 147 111 95 93 74 65 62
58 56 32 7
BspHI T'CATG_A
Cuts at: 3405 3405
Size: 4973
BspMI ACCTGCnnnn'nnnn_
Cuts at: 288 341 1463 1525 2023 2237 2539 2920 288
Size: 53 1122 62 498 214 302 381 2341
Fragments arranged by size:
2341 1122 498 381 302 214 62 53
BsrBI CCG'CTC
Cuts at: 50 642 3265 3319 4803 4968 50
Size: 592 2623 54 1484 165 55
Fragments arranged by size:
2623 1484 592 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2885 2885
Size: 4973
BsrFI r'CCGG_y
Cuts at: 599 898 992 1089 2971 3152 599
Size: 299 94 97 1882 181 2420
Fragments arranged by size:
2420 1882 299 181 97 94
BssHII G'CGCG_C
Cuts at: 741 836 964 1025 1027 1029 741
Size: 95 128 61 2 2 4685
Fragments arranged by size:
4685 128 95 61 2 2
BssSI C'ACGA_G
Cuts at: 1417 1465 3244 3952 1417
Size: 48 1779 708 2438
Fragments arranged by size:
2438 1779 708 48
BstAPI GCAn_nnn'nTGC
Cuts at: 342 723 1346 2350 342
Size: 381 623 1004 2965
Fragments arranged by size:
2965 1004 623 381
BstBI TT'CG_AA
Cuts at: 1296 3334 1296
Size: 2038 2935
BstXI CCAn_nnnn'nTGG
Cuts at: 1350 1350
Size: 4973
BstYI r'GATC_y
Cuts at: 1 1844 1867 1998 2465 2488 2823 3069 3375
Size: 1843 23 131 467 23 335 246 306
Cuts at: 3375 3387 3473 4525 1
Size: 12 86 1052 449
Fragments arranged by size:
1843 1052 467 449 335 306 246 131
86 23 23 12
BtgI C'CryG_G
Cuts at: 123 284 520 647 680 937 1811 1829 1884
Size: 161 236 127 33 257 874 18 55
Cuts at: 1884 123
Size: 3212
Fragments arranged by size:
3212 874 257 236 161 127 55 33
18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 521 2923 3104 3267 521
Size: 2402 181 163 2227
Fragments arranged by size:
2402 2227 181 163
BtsI GCAGTG_nn'
Cuts at: 724 1513 1710 2216 724
Size: 789 197 506 3481
Fragments arranged by size:
3481 789 506 197
DraI TTT'AAA
Cuts at: 1972 1972
Size: 4973
DraIII CAC_nnn'GTG
Cuts at: 239 239
Size: 4973
DrdI GACnn_nn'nnGTC
Cuts at: 2679 4023 2679
Size: 1344 3629
EaeI y'GGCC_r
Cuts at: 115 230 1136 1440 1469 2558 2732 3123 3150
Size: 115 906 304 29 1089 174 391 27
Cuts at: 3150 4495 115
Size: 1345 593
Fragments arranged by size:
1345 1089 906 593 391 304 174 115
29 27
EagI C'GGCC_G
Cuts at: 1136 2558 1136
Size: 1422 3551
EarI CTCTTCn'nnn_
Cuts at: 2996 3206 2996
Size: 210 4763
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 974 3181 3923 4069 4783 974
Size: 2207 742 146 714 1164
Fragments arranged by size:
2207 1164 742 714 146
EcoICRI GAG'CTC
Cuts at: 1416 1929 1985 1416
Size: 513 56 4404
Fragments arranged by size:
4404 513 56
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 815 842 2798 3230 3583 4245 283
Size: 532 27 1956 432 353 662 1011
Fragments arranged by size:
1956 1011 662 532 432 353 27
EcoNI CCTnn'n_nnAGG
Cuts at: 295 295
Size: 4973
EcoO109I rG'GnC_Cy
Cuts at: 826 1001 1050 1164 1656 1657 2112 826
Size: 175 49 114 492 1 455 3687
Fragments arranged by size:
3687 492 455 175 114 49 1
EcoRI G'AATT_C
Cuts at: 1977 1977
Size: 4973
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2992 2992
Size: 4973
FspI TGC'GCA
Cuts at: 2754 2754
Size: 4973
HaeII r_GCGC'y
Cuts at: 415 606 689 777 787 860 875 892 934
Size: 191 83 88 10 73 15 17 42
Cuts at: 934 953 2407 2655 3885 4656 415
Size: 19 1454 248 1230 771 732
Fragments arranged by size:
1454 1230 771 732 248 191 88 83
73 42 19 17 15 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 322 354 391 423 520 552 1263 1295 1852
Size: 32 37 32 97 32 711 32 557
Cuts at: 1852 1859 1884 1891 2815 2845 2847 2877 322
Size: 7 25 7 924 30 2 30 2418
Fragments arranged by size:
2418 924 711 557 97 37 32 32
32 32 30 30 25 7 7 2
HincII GTy'rAC
Cuts at: 732 2012 732
Size: 1280 3693
KasI G'GCGC_C
Cuts at: 411 602 685 783 871 930 949 2403 2651
Size: 191 83 98 88 59 19 1454 248
Cuts at: 2651 411
Size: 2733
Fragments arranged by size:
2733 1454 248 191 98 88 83 59
19
KpnI G_GTAC'C
Cuts at: 1993 1993
Size: 4973
MluI A'CGCG_T
Cuts at: 4406 4406
Size: 4973
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 776 1802 1829 1895 3732 3916 776
Size: 1026 27 66 1837 184 1833
Fragments arranged by size:
1837 1833 1026 184 66 27
MscI TGG'CCA
Cuts at: 1442 1471 2734 1442
Size: 29 1263 3681
Fragments arranged by size:
3681 1263 29
MslI CAynn'nnrTG
Cuts at: 1018 1348 1831 2962 3089 1018
Size: 330 483 1131 127 2902
Fragments arranged by size:
2902 1131 483 330 127
MspA1I CmG'CkG
Cuts at: 215 801 1436 2084 2398 2758 3542 3787 215
Size: 586 635 648 314 360 784 245 1401
Fragments arranged by size:
1401 784 648 635 586 360 314 245
NaeI GCC'GGC
Cuts at: 601 900 3154 601
Size: 299 2254 2420
Fragments arranged by size:
2420 2254 299
NarI GG'CG_CC
Cuts at: 412 603 686 784 872 931 950 2404 2652
Size: 191 83 98 88 59 19 1454 248
Cuts at: 2652 412
Size: 2733
Fragments arranged by size:
2733 1454 248 191 98 88 83 59
19
NgoMIV G'CCGG_C
Cuts at: 599 898 3152 599
Size: 299 2254 2420
Fragments arranged by size:
2420 2254 299
NheI G'CTAG_C
Cuts at: 1900 1900
Size: 4973
NspI r_CATG'y
Cuts at: 576 1308 1449 2026 2526 4129 576
Size: 732 141 577 500 1603 1420
Fragments arranged by size:
1603 1420 732 577 500 141
PasI CC'CwG_GG
Cuts at: 1938 1938
Size: 4973
PciI A'CATG_T
Cuts at: 1445 4125 1445
Size: 2680 2293
PmeI GTTT'AAAC
Cuts at: 1972 1972
Size: 4973
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3419 3451 3419
Size: 32 4941
PpuMI rG'GwC_Cy
Cuts at: 1050 1050
Size: 4973
PspOMI G'GGCC_C
Cuts at: 1000 1163 1656 1000
Size: 163 493 4317
Fragments arranged by size:
4317 493 163
PstI C_TGCA'G
Cuts at: 338 1508 1959 2020 2705 338
Size: 1170 451 61 685 2606
Fragments arranged by size:
2606 1170 685 451 61
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4973
PvuII CAG'CTG
Cuts at: 2398 2758 2398
Size: 360 4613
RsrII CG'GwC_CG
Cuts at: 3168 3168
Size: 4973
SacI G_AGCT'C
Cuts at: 1418 1931 1987 1418
Size: 513 56 4404
Fragments arranged by size:
4404 513 56
SalI G'TCGA_C
Cuts at: 2010 2010
Size: 4973
SanDI GG'GwC_CC
Cuts at: 1050 1050
Size: 4973
SapI GCTCTTCn'nnn_
Cuts at: 2996 3206 2996
Size: 210 4763
SbfI CC_TGCA'GG
Cuts at: 2020 2020
Size: 4973
ScaI AGT'ACT
Cuts at: 4490 4490
Size: 4973
SfcI C'TryA_G
Cuts at: 33 334 1504 1955 2016 2701 3669 3860 33
Size: 301 1170 451 61 685 968 191 1146
Fragments arranged by size:
1170 1146 968 685 451 301 191 61
SfiI GGCCn_nnn'nGGCC
Cuts at: 1430 1439 1430
Size: 9 4964
SfoI GGC'GCC
Cuts at: 413 604 687 785 873 932 951 2405 2653
Size: 191 83 98 88 59 19 1454 248
Cuts at: 2653 413
Size: 2733
Fragments arranged by size:
2733 1454 248 191 98 88 83 59
19
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4973
SgrAI Cr'CCGG_yG
Cuts at: 599 599
Size: 4973
SmaI CCC'GGG
Cuts at: 178 927 947 974 999 1233 1677 1995 178
Size: 749 20 27 25 234 444 318 3156
Fragments arranged by size:
3156 749 444 318 234 27 25 20
SmlI C'TyrA_G
Cuts at: 2128 3043 3757 4019 2128
Size: 915 714 262 3082
Fragments arranged by size:
3082 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4973
SphI G_CATG'C
Cuts at: 2026 2526 2026
Size: 500 4473
SrfI GCCC'GGGC
Cuts at: 947 999 947
Size: 52 4921
StyI C'CwwG_G
Cuts at: 1961 2107 2461 1961
Size: 146 354 4473
Fragments arranged by size:
4473 354 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1873 2556 2689 3148 3357 1873
Size: 683 133 459 209 3489
Fragments arranged by size:
3489 683 459 209 133
TatI w'GTAC_w
Cuts at: 1475 4488 1475
Size: 3013 1960
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4490 4522 4490
Size: 32 4941
Tth111I GACn'n_nGTC
Cuts at: 2770 2770
Size: 4973
XbaI T'CTAG_A
Cuts at: 2004 2004
Size: 4973
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 759 2468 759
Size: 1709 3264
XmaI C'CCGG_G
Cuts at: 176 925 945 972 997 1231 1675 1993 176
Size: 749 20 27 25 234 444 318 3156
Fragments arranged by size:
3156 749 444 318 234 27 25 20
XmnI GAAnn'nnTTC
Cuts at: 1684 1684
Size: 4973
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NheI NspI
PasI PciI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI SrfI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AlfI AloI AscI AvrII
BbvCI BfrBI BsaXI BseRI BsiWI BsmI BsmBI BspEI
BsrGI BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NotI NruI
NsiI PacI PflMI PfoI PmlI PshAI PsiI PspXI
PsrI SacII SexAI SnaBI SspI StuI SwaI XhoI
ZraI