Restriction map for
pF1KB3230
(Circular) (Six-base) MAPSORT of: pF1KB3230.seq Check: 2970 from: 1 to: 5000
pF1KB3230 5000 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 289 289
Size: 5000
AatII G_ACGT'C
Cuts at: 1886 1886
Size: 5000
AccI GT'mk_AC
Cuts at: 2038 2038
Size: 5000
Acc65I G'GTAC_C
Cuts at: 2016 2016
Size: 5000
AclI AA'CG_TT
Cuts at: 4840 4840
Size: 5000
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 973 1504 2825 3257 3610 4272 973
Size: 531 1321 432 353 662 1701
Fragments arranged by size:
1701 1321 662 531 432 353
AflIII A'CryG_T
Cuts at: 741 2352 4152 4433 741
Size: 1611 1800 281 1308
Fragments arranged by size:
1800 1611 1308 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1696 1728 1696
Size: 32 4968
AlwNI CAG_nnn'CTG
Cuts at: 194 2305 3743 194
Size: 2111 1438 1451
Fragments arranged by size:
2111 1451 1438
ApaI G_GGCC'C
Cuts at: 389 389
Size: 5000
ApaLI G'TGCA_C
Cuts at: 1342 3838 1342
Size: 2496 2504
ApoI r'AATT_y
Cuts at: 2004 4353 4609 2004
Size: 2349 256 2395
Fragments arranged by size:
2395 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5000
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5000
AvaI C'yCGr_G
Cuts at: 1094 2020 1094
Size: 926 4074
BamHI G'GATC_C
Cuts at: 480 1458 2025 480
Size: 978 567 3455
Fragments arranged by size:
3455 978 567
BanI G'GyrC_C
Cuts at: 1360 2016 2430 2678 2713 1360
Size: 656 414 248 35 3647
Fragments arranged by size:
3647 656 414 248 35
BanII G_rGCy'C
Cuts at: 155 326 389 1166 1241 1955 2014 3044 155
Size: 171 63 777 75 714 59 1030 2111
Fragments arranged by size:
2111 1030 777 714 171 75 63 59
BbeI G_GCGC'C
Cuts at: 2434 2682 2434
Size: 248 4752
BbsI GAAGACnn'nnnn_
Cuts at: 508 1438 508
Size: 930 4070
BbvCI CC'TCA_GC
Cuts at: 301 301
Size: 5000
BciVI GTATCCnnnnn_n'
Cuts at: 534 2898 3954 5000 534
Size: 2364 1056 1046 534
Fragments arranged by size:
2364 1056 1046 534
BclI T'GATC_A
Cuts at: 963 2520 963
Size: 1557 3443
BfrBI ATG'CAT
Cuts at: 757 757
Size: 5000
BglII A'GATC_T
Cuts at: 2515 4552 2515
Size: 2037 2963
BlpI GC'TnA_GC
Cuts at: 881 2112 881
Size: 1231 3769
Bme1580I G_kGCm'C
Cuts at: 277 389 1346 2625 2718 3842 277
Size: 112 957 1279 93 1124 1435
Fragments arranged by size:
1435 1279 1124 957 112 93
BmgBI CAC'GTC
Cuts at: 763 763
Size: 5000
BmrI ACTGGGnnnn_n'
Cuts at: 1752 1958 2212 2293 2628 4772 1752
Size: 206 254 81 335 2144 1980
Fragments arranged by size:
2144 1980 335 254 206 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1026 1180 3367 1026
Size: 154 2187 2659
Fragments arranged by size:
2659 2187 154
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 169 724 1225 139
Size: 30 555 501 3914
Fragments arranged by size:
3914 555 501 30
Bpu10I CC'TnA_GC
Cuts at: 301 301
Size: 5000
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 2176 3055 3769 4067 310
Size: 1866 879 714 298 1243
Fragments arranged by size:
1866 1243 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 397 700 1233 4704 397
Size: 303 533 3471 693
Fragments arranged by size:
3471 693 533 303
BsaAI yAC'GTr
Cuts at: 2353 2353
Size: 5000
BsaBI GATnn'nnATC
Cuts at: 6 2514 4551 6
Size: 2508 2037 455
Fragments arranged by size:
2508 2037 455
BsaHI Gr'CG_yC
Cuts at: 428 1883 2431 2679 3381 4877 428
Size: 1455 548 248 702 1496 551
Fragments arranged by size:
1496 1455 702 551 548 248
BsaWI w'CCGG_w
Cuts at: 603 1258 2189 2710 3388 3799 3946 4220 603
Size: 655 931 521 678 411 147 274 1383
Fragments arranged by size:
1383 931 678 655 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 799 799
Size: 5000
BseYI C'CCAG_C
Cuts at: 397 2425 3183 3848 4256 397
Size: 2028 758 665 408 1141
Fragments arranged by size:
2028 1141 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1152 1962 1152
Size: 810 4190
BsiEI CG_ry'CG
Cuts at: 104 2542 2588 3818 104
Size: 2438 46 1230 1286
Fragments arranged by size:
2438 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 155 1166 1241 1346 2014 2792 2982 3842 155
Size: 1011 75 105 668 778 190 860 1313
Fragments arranged by size:
1313 1011 860 778 668 190 105 75
Bsp1286I G_dGCh'C
Cuts at: 155 277 326 389 1166 1241 1346 1955 2014
Size: 122 49 63 777 75 105 609 59
Cuts at: 2014 2625 2718 2792 2982 3044 3842 155
Size: 611 93 74 190 62 798 1313
Fragments arranged by size:
1313 798 777 611 609 190 122 105
93 75 74 63 62 59 49
BspEI T'CCGG_A
Cuts at: 603 603
Size: 5000
BspHI T'CATG_A
Cuts at: 3432 3432
Size: 5000
BspMI ACCTGCnnnn'nnnn_
Cuts at: 142 289 2050 2264 2566 2947 142
Size: 147 1761 214 302 381 2195
Fragments arranged by size:
2195 1761 381 302 214 147
BsrBI CCG'CTC
Cuts at: 50 383 417 915 1358 3292 3346 4830 4995
Size: 333 34 498 443 1934 54 1484 165
Cuts at: 4995 50
Size: 55
Fragments arranged by size:
1934 1484 498 443 333 165 55 54
34
BsrDI GCAATG_nn'
Cuts at: 2912 2912
Size: 5000
BsrFI r'CCGG_y
Cuts at: 284 1610 1992 2998 3179 284
Size: 1326 382 1006 181 2105
Fragments arranged by size:
2105 1326 1006 382 181
BssSI C'ACGA_G
Cuts at: 3271 3979 3271
Size: 708 4292
BstAPI GCAn_nnn'nTGC
Cuts at: 905 1350 2377 905
Size: 445 1027 3528
Fragments arranged by size:
3528 1027 445
BstBI TT'CG_AA
Cuts at: 3361 3361
Size: 5000
BstYI r'GATC_y
Cuts at: 1 480 1458 2025 2492 2515 2850 3096 3402
Size: 479 978 567 467 23 335 246 306
Cuts at: 3402 3414 3500 4552 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 978 567 479 467 449 335 306
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 1013 107
Size: 906 4094
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2950 3131 3294 2950
Size: 181 163 4656
Fragments arranged by size:
4656 181 163
BtsI GCAGTG_nn'
Cuts at: 1419 2243 1419
Size: 824 4176
DraI TTT'AAA
Cuts at: 1452 1999 1452
Size: 547 4453
DraIII CAC_nnn'GTG
Cuts at: 232 1922 232
Size: 1690 3310
DrdI GACnn_nn'nnGTC
Cuts at: 2706 4050 2706
Size: 1344 3656
EaeI y'GGCC_r
Cuts at: 1608 2585 2759 3150 3177 4522 1608
Size: 977 174 391 27 1345 2086
Fragments arranged by size:
2086 1345 977 391 174 27
EagI C'GGCC_G
Cuts at: 2585 2585
Size: 5000
EarI CTCTTCn'nnn_
Cuts at: 173 1154 1531 3023 3233 173
Size: 981 377 1492 210 1940
Fragments arranged by size:
1940 1492 981 377 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 435 3208 3950 4096 4810 435
Size: 2773 742 146 714 625
Fragments arranged by size:
2773 742 714 625 146
EcoICRI GAG'CTC
Cuts at: 153 1164 1239 2012 153
Size: 1011 75 773 3141
Fragments arranged by size:
3141 1011 773 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 169 724 973 1225 1504 2825 3257 3610
Size: 30 555 249 252 279 1321 432 353
Cuts at: 3610 4272 139
Size: 662 867
Fragments arranged by size:
1321 867 662 555 432 353 279 252
249 30
EcoO109I rG'GnC_Cy
Cuts at: 162 456 2139 162
Size: 294 1683 3023
Fragments arranged by size:
3023 1683 294
EcoRI G'AATT_C
Cuts at: 2004 2004
Size: 5000
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 588 675 1389 3019 588
Size: 87 714 1630 2569
Fragments arranged by size:
2569 1630 714 87
FspI TGC'GCA
Cuts at: 183 1895 2781 183
Size: 1712 886 2402
Fragments arranged by size:
2402 1712 886
HaeII r_GCGC'y
Cuts at: 1723 2434 2682 3912 4683 1723
Size: 711 248 1230 771 2040
Fragments arranged by size:
2040 1230 771 711 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1450 1482 1510 1513 1542 1545 1816 1848 2842
Size: 32 28 3 29 3 271 32 994
Cuts at: 2842 2872 2874 2904 1450
Size: 30 2 30 3546
Fragments arranged by size:
3546 994 271 32 32 30 30 29
28 3 3 2
HincII GTy'rAC
Cuts at: 739 2039 739
Size: 1300 3700
KasI G'GCGC_C
Cuts at: 2430 2678 2430
Size: 248 4752
KpnI G_GTAC'C
Cuts at: 2020 2020
Size: 5000
MluI A'CGCG_T
Cuts at: 4433 4433
Size: 5000
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3759 3943 3759
Size: 184 4816
MscI TGG'CCA
Cuts at: 2761 2761
Size: 5000
MslI CAynn'nnrTG
Cuts at: 644 1192 1375 2989 3116 644
Size: 548 183 1614 127 2528
Fragments arranged by size:
2528 1614 548 183 127
MspA1I CmG'CkG
Cuts at: 1730 1989 2111 2425 2785 3569 3814 1730
Size: 259 122 314 360 784 245 2916
Fragments arranged by size:
2916 784 360 314 259 245 122
NaeI GCC'GGC
Cuts at: 286 1612 3181 286
Size: 1326 1569 2105
Fragments arranged by size:
2105 1569 1326
NarI GG'CG_CC
Cuts at: 2431 2679 2431
Size: 248 4752
NcoI C'CATG_G
Cuts at: 107 1013 107
Size: 906 4094
NdeI CA'TA_TG
Cuts at: 1179 1179
Size: 5000
NgoMIV G'CCGG_C
Cuts at: 284 1610 3179 284
Size: 1326 1569 2105
Fragments arranged by size:
2105 1569 1326
NsiI A_TGCA'T
Cuts at: 759 759
Size: 5000
NspI r_CATG'y
Cuts at: 292 745 2053 2553 4156 292
Size: 453 1308 500 1603 1136
Fragments arranged by size:
1603 1308 1136 500 453
PciI A'CATG_T
Cuts at: 741 4152 741
Size: 3411 1589
PmeI GTTT'AAAC
Cuts at: 1999 1999
Size: 5000
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3446 3478 3446
Size: 32 4968
PpuMI rG'GwC_Cy
Cuts at: 162 456 162
Size: 294 4706
PshAI GACnn'nnGTC
Cuts at: 508 508
Size: 5000
PspOMI G'GGCC_C
Cuts at: 385 385
Size: 5000
PstI C_TGCA'G
Cuts at: 1355 2047 2732 1355
Size: 692 685 3623
Fragments arranged by size:
3623 692 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5000
PvuII CAG'CTG
Cuts at: 1989 2425 2785 1989
Size: 436 360 4204
Fragments arranged by size:
4204 436 360
RsrII CG'GwC_CG
Cuts at: 3195 3195
Size: 5000
SacI G_AGCT'C
Cuts at: 155 1166 1241 2014 155
Size: 1011 75 773 3141
Fragments arranged by size:
3141 1011 773 75
SalI G'TCGA_C
Cuts at: 2037 2037
Size: 5000
SapI GCTCTTCn'nnn_
Cuts at: 1154 3023 3233 1154
Size: 1869 210 2921
Fragments arranged by size:
2921 1869 210
SbfI CC_TGCA'GG
Cuts at: 2047 2047
Size: 5000
ScaI AGT'ACT
Cuts at: 4517 4517
Size: 5000
SfcI C'TryA_G
Cuts at: 33 343 979 1351 2043 2728 3696 3887 33
Size: 310 636 372 692 685 968 191 1146
Fragments arranged by size:
1146 968 692 685 636 372 310 191
SfoI GGC'GCC
Cuts at: 2432 2680 2432
Size: 248 4752
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5000
SmaI CCC'GGG
Cuts at: 2022 2022
Size: 5000
SmlI C'TyrA_G
Cuts at: 325 2155 3070 3784 4046 325
Size: 1830 915 714 262 1279
Fragments arranged by size:
1830 1279 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5000
SphI G_CATG'C
Cuts at: 2053 2553 2053
Size: 500 4500
StyI C'CwwG_G
Cuts at: 107 1013 2134 2488 107
Size: 906 1121 354 2619
Fragments arranged by size:
2619 1121 906 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1734 2583 2716 3175 3384 1734
Size: 849 133 459 209 3350
Fragments arranged by size:
3350 849 459 209 133
TatI w'GTAC_w
Cuts at: 1244 1367 4515 1244
Size: 123 3148 1729
Fragments arranged by size:
3148 1729 123
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4517 4549 4517
Size: 32 4968
Tth111I GACn'n_nGTC
Cuts at: 2797 2797
Size: 5000
XbaI T'CTAG_A
Cuts at: 315 2031 315
Size: 1716 3284
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 246 1573 2495 114
Size: 132 1327 922 2619
Fragments arranged by size:
2619 1327 922 132
XmaI C'CCGG_G
Cuts at: 2020 2020
Size: 5000
ZraI GAC'GTC
Cuts at: 1884 1884
Size: 5000
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AleI AlfI AscI AvrII
BaeI BcgI BglI BmtI BsaXI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NheI
NotI NruI PacI PasI PflMI PfoI PmlI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI