Restriction map for
pF1KB3232
(Circular) (Six-base) MAPSORT of: pF1KB3232.seq Check: 902 from: 1 to: 5252
pF1KB3232 5252 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1115 1515 1115
Size: 400 4852
AccI GT'mk_AC
Cuts at: 229 2290 229
Size: 2061 3191
Acc65I G'GTAC_C
Cuts at: 174 1526 2268 174
Size: 1352 742 3158
Fragments arranged by size:
3158 1352 742
AclI AA'CG_TT
Cuts at: 5092 5092
Size: 5252
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1855 3077 3509 3862 4524 1855
Size: 1222 432 353 662 2583
Fragments arranged by size:
2583 1222 662 432 353
AflII C'TTAA_G
Cuts at: 967 967
Size: 5252
AflIII A'CryG_T
Cuts at: 2604 4404 4685 2604
Size: 1800 281 3171
Fragments arranged by size:
3171 1800 281
AleI CACnn'nnGTG
Cuts at: 1597 1597
Size: 5252
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 625 796 1450 625
Size: 171 654 4427
Fragments arranged by size:
4427 654 171
AlwNI CAG_nnn'CTG
Cuts at: 327 465 656 1611 1692 2557 3995 327
Size: 138 191 955 81 865 1438 1584
Fragments arranged by size:
1584 1438 955 865 191 138 81
ApaLI G'TGCA_C
Cuts at: 4090 4090
Size: 5252
ApoI r'AATT_y
Cuts at: 2241 2256 4605 4861 2241
Size: 15 2349 256 2632
Fragments arranged by size:
2632 2349 256 15
AseI AT'TA_AT
Cuts at: 20 20
Size: 5252
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5252
AvaI C'yCGr_G
Cuts at: 431 857 889 1036 1465 1540 1835 2272 431
Size: 426 32 147 429 75 295 437 3411
Fragments arranged by size:
3411 437 429 426 295 147 75 32
BamHI G'GATC_C
Cuts at: 2277 2277
Size: 5252
BanI G'GyrC_C
Cuts at: 151 174 861 885 1258 1338 1526 2268 2682
Size: 23 687 24 373 80 188 742 414
Cuts at: 2682 2930 2965 151
Size: 248 35 2438
Fragments arranged by size:
2438 742 687 414 373 248 188 80
35 24 23
BanII G_rGCy'C
Cuts at: 896 1700 2000 2266 3296 896
Size: 804 300 266 1030 2852
Fragments arranged by size:
2852 1030 804 300 266
BbeI G_GCGC'C
Cuts at: 2686 2934 2686
Size: 248 5004
BbvCI CC'TCA_GC
Cuts at: 598 598
Size: 5252
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1000 1034 1000
Size: 34 5218
BciVI GTATCCnnnnn_n'
Cuts at: 3150 4206 5252 3150
Size: 1056 1046 3150
Fragments arranged by size:
3150 1056 1046
BclI T'GATC_A
Cuts at: 2772 2772
Size: 5252
BglI GCCn_nnn'nGGC
Cuts at: 583 2011 583
Size: 1428 3824
BglII A'GATC_T
Cuts at: 788 1469 2767 4804 788
Size: 681 1298 2037 1236
Fragments arranged by size:
2037 1298 1236 681
BlpI GC'TnA_GC
Cuts at: 466 2364 466
Size: 1898 3354
Bme1580I G_kGCm'C
Cuts at: 170 866 876 890 1261 1343 1449 2136 2877
Size: 696 10 14 371 82 106 687 741
Cuts at: 2877 2970 4094 170
Size: 93 1124 1328
Fragments arranged by size:
1328 1124 741 696 687 371 106 93
82 14 10
BmrI ACTGGGnnnn_n'
Cuts at: 1485 1896 1920 2464 2545 2880 5024 1485
Size: 411 24 544 81 335 2144 1713
Fragments arranged by size:
2144 1713 544 411 335 81 24
BmtI G_CTAG'C
Cuts at: 139 769 2047 139
Size: 630 1278 3344
Fragments arranged by size:
3344 1278 630
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 849 1020 2114 3619 849
Size: 171 1094 1505 2482
Fragments arranged by size:
2482 1505 1094 171
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 826 844 910 1300 1390 1469 1573 1648
Size: 303 18 66 390 90 79 104 75
Cuts at: 1648 1714 2090 2148 523
Size: 66 376 58 3627
Fragments arranged by size:
3627 390 376 303 104 90 79 75
66 66 58 18
Bpu10I CC'TnA_GC
Cuts at: 598 598
Size: 5252
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 880 2428 3307 4021 4319 880
Size: 1548 879 714 298 1813
Fragments arranged by size:
1813 1548 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1008 1114 1207 1705 4956 1008
Size: 106 93 498 3251 1304
Fragments arranged by size:
3251 1304 498 106 93
BsaAI yAC'GTr
Cuts at: 2605 2605
Size: 5252
BsaBI GATnn'nnATC
Cuts at: 6 2766 4803 6
Size: 2760 2037 455
Fragments arranged by size:
2760 2037 455
BsaHI Gr'CG_yC
Cuts at: 2683 2931 3633 5129 2683
Size: 248 702 1496 2806
Fragments arranged by size:
2806 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1308 2441 2962 3640 4051 4198 4472 1308
Size: 1133 521 678 411 147 274 2088
Fragments arranged by size:
2088 1133 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 610 640 2206 2236 610
Size: 30 1566 30 3626
Fragments arranged by size:
3626 1566 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 496 1009 1651 1782 496
Size: 513 642 131 3966
Fragments arranged by size:
3966 642 513 131
BseYI C'CCAG_C
Cuts at: 372 458 975 1241 1489 2038 2677 3435 4100
Size: 86 517 266 248 549 639 758 665
Cuts at: 4100 4508 372
Size: 408 1116
Fragments arranged by size:
1116 758 665 639 549 517 408 266
248 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 601 946 1726 457
Size: 144 345 780 3983
Fragments arranged by size:
3983 780 345 144
BsiEI CG_ry'CG
Cuts at: 104 2794 2840 4070 104
Size: 2690 46 1230 1286
Fragments arranged by size:
2690 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1659 1700 2266 3044 3234 4094 1659
Size: 41 566 778 190 860 2817
Fragments arranged by size:
2817 860 778 566 190 41
Bsp1286I G_dGCh'C
Cuts at: 170 866 876 890 896 1261 1343 1449 1659
Size: 696 10 14 6 365 82 106 210
Cuts at: 1659 1700 2000 2136 2266 2877 2970 3044 3234
Size: 41 300 136 130 611 93 74 190
Cuts at: 3234 3296 4094 170
Size: 62 798 1328
Fragments arranged by size:
1328 798 696 611 365 300 210 190
136 130 106 93 82 74 62 41
14 10 6
BspEI T'CCGG_A
Cuts at: 1308 1308
Size: 5252
BspHI T'CATG_A
Cuts at: 689 3684 689
Size: 2995 2257
BspMI ACCTGCnnnn'nnnn_
Cuts at: 758 788 1115 1196 1515 2302 2516 2818 3199
Size: 30 327 81 319 787 214 302 381
Cuts at: 3199 758
Size: 2811
Fragments arranged by size:
2811 787 381 327 319 302 214 81
30
BsrBI CCG'CTC
Cuts at: 50 239 924 3544 3598 5082 5247 50
Size: 189 685 2620 54 1484 165 55
Fragments arranged by size:
2620 1484 685 189 165 55 54
BsrDI GCAATG_nn'
Cuts at: 279 1437 2000 3164 279
Size: 1158 563 1164 2367
Fragments arranged by size:
2367 1164 1158 563
BsrFI r'CCGG_y
Cuts at: 3250 3431 3250
Size: 181 5071
BssSI C'ACGA_G
Cuts at: 118 3523 4231 118
Size: 3405 708 1139
Fragments arranged by size:
3405 1139 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2629 2629
Size: 5252
BstBI TT'CG_AA
Cuts at: 3613 3613
Size: 5252
BstEII G'GTnAC_C
Cuts at: 1743 1743
Size: 5252
BstXI CCAn_nnnn'nTGG
Cuts at: 1821 1821
Size: 5252
BstYI r'GATC_y
Cuts at: 1 215 788 1469 2200 2277 2744 2767 3102
Size: 214 573 681 731 77 467 23 335
Cuts at: 3102 3348 3654 3666 3752 4804 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 731 681 573 467 449 335 306
246 214 86 77 23 12
BstZ17I GTA'TAC
Cuts at: 230 230
Size: 5252
Bsu36I CC'TnA_GG
Cuts at: 637 1980 637
Size: 1343 3909
BtgI C'CryG_G
Cuts at: 170 1069 1206 1253 1877 170
Size: 899 137 47 624 3545
Fragments arranged by size:
3545 899 624 137 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 558 3202 3383 3546 558
Size: 2644 181 163 2264
Fragments arranged by size:
2644 2264 181 163
BtsI GCAGTG_nn'
Cuts at: 155 2495 155
Size: 2340 2912
DraI TTT'AAA
Cuts at: 2251 2251
Size: 5252
DraIII CAC_nnn'GTG
Cuts at: 1295 1295
Size: 5252
DrdI GACnn_nn'nnGTC
Cuts at: 1173 2958 4302 1173
Size: 1785 1344 2123
Fragments arranged by size:
2123 1785 1344
EaeI y'GGCC_r
Cuts at: 383 527 743 1451 2837 3011 3402 3429 4774
Size: 144 216 708 1386 174 391 27 1345
Cuts at: 4774 383
Size: 861
Fragments arranged by size:
1386 1345 861 708 391 216 174 144
27
EagI C'GGCC_G
Cuts at: 2837 2837
Size: 5252
EarI CTCTTCn'nnn_
Cuts at: 1571 3275 3485 1571
Size: 1704 210 3338
Fragments arranged by size:
3338 1704 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3460 4202 4348 5062 3460
Size: 742 146 714 3650
Fragments arranged by size:
3650 742 714 146
EcoICRI GAG'CTC
Cuts at: 1698 2264 1698
Size: 566 4686
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 826 844 910 1300 1390 1469 1573 1648
Size: 303 18 66 390 90 79 104 75
Cuts at: 1648 1714 1855 2090 2148 3077 3509 3862 4524
Size: 66 141 235 58 929 432 353 662
Cuts at: 4524 523
Size: 1251
Fragments arranged by size:
1251 929 662 432 390 353 303 235
141 104 90 79 75 66 66 58
18
EcoNI CCTnn'n_nnAGG
Cuts at: 1192 1870 1192
Size: 678 4574
EcoO109I rG'GnC_Cy
Cuts at: 584 1547 2391 584
Size: 963 844 3445
Fragments arranged by size:
3445 963 844
EcoRI G'AATT_C
Cuts at: 2256 2256
Size: 5252
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3271 3271
Size: 5252
FspI TGC'GCA
Cuts at: 3033 3033
Size: 5252
HaeII r_GCGC'y
Cuts at: 1323 2686 2934 4164 4935 1323
Size: 1363 248 1230 771 1640
Fragments arranged by size:
1640 1363 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1270 1302 3094 3124 3126 3156 1270
Size: 32 1792 30 2 30 3366
Fragments arranged by size:
3366 1792 32 30 30 2
HincII GTy'rAC
Cuts at: 2291 2291
Size: 5252
HindIII A'AGCT_T
Cuts at: 1389 1389
Size: 5252
KasI G'GCGC_C
Cuts at: 2682 2930 2682
Size: 248 5004
KpnI G_GTAC'C
Cuts at: 178 1530 2272 178
Size: 1352 742 3158
Fragments arranged by size:
3158 1352 742
MluI A'CGCG_T
Cuts at: 4685 4685
Size: 5252
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 653 818 4011 4195 653
Size: 165 3193 184 1710
Fragments arranged by size:
3193 1710 184 165
MscI TGG'CCA
Cuts at: 385 529 745 3013 385
Size: 144 216 2268 2624
Fragments arranged by size:
2624 2268 216 144
MslI CAynn'nnrTG
Cuts at: 1597 3241 3368 1597
Size: 1644 127 3481
Fragments arranged by size:
3481 1644 127
MspA1I CmG'CkG
Cuts at: 462 492 656 852 930 1223 1241 1489 2038
Size: 30 164 196 78 293 18 248 549
Cuts at: 2038 2363 2677 3037 3821 4066 462
Size: 325 314 360 784 245 1648
Fragments arranged by size:
1648 784 549 360 325 314 293 248
245 196 164 78 30 18
NaeI GCC'GGC
Cuts at: 3433 3433
Size: 5252
NarI GG'CG_CC
Cuts at: 2683 2931 2683
Size: 248 5004
NgoMIV G'CCGG_C
Cuts at: 3431 3431
Size: 5252
NheI G'CTAG_C
Cuts at: 135 765 2043 135
Size: 630 1278 3344
Fragments arranged by size:
3344 1278 630
NspI r_CATG'y
Cuts at: 187 960 2305 2805 4408 187
Size: 773 1345 500 1603 1031
Fragments arranged by size:
1603 1345 1031 773 500
PasI CC'CwG_GG
Cuts at: 581 581
Size: 5252
PciI A'CATG_T
Cuts at: 4404 4404
Size: 5252
PflMI CCAn_nnn'nTGG
Cuts at: 1611 1611
Size: 5252
PfoI T'CCnGG_A
Cuts at: 719 1346 719
Size: 627 4625
PmeI GTTT'AAAC
Cuts at: 2251 2251
Size: 5252
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 533 565 3698 3730 533
Size: 32 3133 32 2055
Fragments arranged by size:
3133 2055 32 32
PpuMI rG'GwC_Cy
Cuts at: 1547 1547
Size: 5252
PshAI GACnn'nnGTC
Cuts at: 292 292
Size: 5252
PstI C_TGCA'G
Cuts at: 338 1193 2299 2984 338
Size: 855 1106 685 2606
Fragments arranged by size:
2606 1106 855 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5252
PvuII CAG'CTG
Cuts at: 462 492 852 1489 2038 2677 3037 462
Size: 30 360 637 549 639 360 2677
Fragments arranged by size:
2677 639 637 549 360 360 30
RsrII CG'GwC_CG
Cuts at: 1329 3447 1329
Size: 2118 3134
SacI G_AGCT'C
Cuts at: 1700 2266 1700
Size: 566 4686
SalI G'TCGA_C
Cuts at: 2289 2289
Size: 5252
SanDI GG'GwC_CC
Cuts at: 1547 1547
Size: 5252
SapI GCTCTTCn'nnn_
Cuts at: 3275 3485 3275
Size: 210 5042
SbfI CC_TGCA'GG
Cuts at: 1193 2299 1193
Size: 1106 4146
ScaI AGT'ACT
Cuts at: 1885 4769 1885
Size: 2884 2368
SexAI A'CCwGG_T
Cuts at: 1610 1777 1610
Size: 167 5085
SfcI C'TryA_G
Cuts at: 33 334 535 1189 2295 2980 3948 4139 33
Size: 301 201 654 1106 685 968 191 1146
Fragments arranged by size:
1146 1106 968 685 654 301 201 191
SfoI GGC'GCC
Cuts at: 2684 2932 2684
Size: 248 5004
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5252
SmaI CCC'GGG
Cuts at: 859 891 2274 859
Size: 32 1383 3837
Fragments arranged by size:
3837 1383 32
SmlI C'TyrA_G
Cuts at: 895 967 2407 3322 4036 4298 895
Size: 72 1440 915 714 262 1849
Fragments arranged by size:
1849 1440 915 714 262 72
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5252
SphI G_CATG'C
Cuts at: 2305 2805 2305
Size: 500 4752
SrfI GCCC'GGGC
Cuts at: 891 891
Size: 5252
StyI C'CwwG_G
Cuts at: 2055 2386 2740 2055
Size: 331 354 4567
Fragments arranged by size:
4567 354 331
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 132 1767 1939 2074 2835 2968 3427 3636 132
Size: 1635 172 135 761 133 459 209 1748
Fragments arranged by size:
1748 1635 761 459 209 172 135 133
TatI w'GTAC_w
Cuts at: 1883 4767 1883
Size: 2884 2368
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 610 642 1376 1408 4769 4801 610
Size: 32 734 32 3361 32 1061
Fragments arranged by size:
3361 1061 734 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1231 3049 1231
Size: 1818 3434
XbaI T'CTAG_A
Cuts at: 2283 2283
Size: 5252
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 168 873 1414 2079 2747 168
Size: 705 541 665 668 2673
Fragments arranged by size:
2673 705 668 665 541
XmaI C'CCGG_G
Cuts at: 857 889 2272 857
Size: 32 1383 3837
Fragments arranged by size:
3837 1383 32
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NheI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AloI ApaI AscI AvrII
BaeI BbsI BfrBI BmgBI BsiWI BsmI BsmBI BsrGI
BssHII ClaI CspCI EcoRV FseI FspAI HpaI MfeI
NcoI NdeI NotI NruI NsiI PacI PmlI PsiI
PspOMI PspXI PsrI SacII SfiI SgrAI SnaBI SspI
StuI SwaI XhoI XmnI ZraI