Restriction map for
pF1KB3236
(Circular) (Six-base) MAPSORT of: pF1KB3236.seq Check: 8778 from: 1 to: 4829
pF1KB3236 4829 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 1867 1867
Size: 4829
Acc65I G'GTAC_C
Cuts at: 1845 1845
Size: 4829
AclI AA'CG_TT
Cuts at: 4669 4669
Size: 4829
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 494 1808 2654 3086 3833 4101 494
Size: 1314 846 432 747 268 1222
Fragments arranged by size:
1314 1222 846 747 432 268
AflIII A'CryG_T
Cuts at: 2181 3285 4262 2181
Size: 1104 977 2748
Fragments arranged by size:
2748 1104 977
AgeI A'CCGG_T
Cuts at: 3217 3217
Size: 4829
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1380 1380
Size: 4829
AlwNI CAG_nnn'CTG
Cuts at: 931 2134 3701 931
Size: 1203 1567 2059
Fragments arranged by size:
2059 1567 1203
ApaLI G'TGCA_C
Cuts at: 3599 3599
Size: 4829
ApoI r'AATT_y
Cuts at: 288 394 787 1274 1833 4182 4438 288
Size: 106 393 487 559 2349 256 679
Fragments arranged by size:
2349 679 559 487 393 256 106
AseI AT'TA_AT
Cuts at: 20 1123 20
Size: 1103 3726
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4829
AvaI C'yCGr_G
Cuts at: 1849 1849
Size: 4829
AvrII C'CTAG_G
Cuts at: 1026 1026
Size: 4829
BamHI G'GATC_C
Cuts at: 1854 1854
Size: 4829
BanI G'GyrC_C
Cuts at: 1845 2259 2507 2542 1845
Size: 414 248 35 4132
Fragments arranged by size:
4132 414 248 35
BanII G_rGCy'C
Cuts at: 116 1843 2873 116
Size: 1727 1030 2072
Fragments arranged by size:
2072 1727 1030
BbeI G_GCGC'C
Cuts at: 2263 2511 2263
Size: 248 4581
BbvCI CC'TCA_GC
Cuts at: 1553 1553
Size: 4829
BciVI GTATCCnnnnn_n'
Cuts at: 2727 3488 4829 2727
Size: 761 1341 2727
Fragments arranged by size:
2727 1341 761
BclI T'GATC_A
Cuts at: 987 2349 987
Size: 1362 3467
BfrBI ATG'CAT
Cuts at: 523 862 1669 523
Size: 339 807 3683
Fragments arranged by size:
3683 807 339
BglII A'GATC_T
Cuts at: 2344 4381 2344
Size: 2037 2792
BlpI GC'TnA_GC
Cuts at: 1565 1941 1565
Size: 376 4453
Bme1580I G_kGCm'C
Cuts at: 2454 2547 3603 2454
Size: 93 1056 3680
Fragments arranged by size:
3680 1056 93
BmgBI CAC'GTC
Cuts at: 1138 1387 1138
Size: 249 4580
BmrI ACTGGGnnnn_n'
Cuts at: 2041 2122 2457 4601 2041
Size: 81 335 2144 2269
Fragments arranged by size:
2269 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3196 3196
Size: 4829
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1034 1505 1034
Size: 471 4358
Bpu10I CC'TnA_GC
Cuts at: 1553 1553
Size: 4829
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 647 2005 2884 3376 3674 647
Size: 1358 879 492 298 1802
Fragments arranged by size:
1802 1358 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 478 581 952 4533 478
Size: 103 371 3581 774
Fragments arranged by size:
3581 774 371 103
BsaAI yAC'GTr
Cuts at: 2182 2182
Size: 4829
BsaBI GATnn'nnATC
Cuts at: 6 2343 4380 6
Size: 2337 2037 455
Fragments arranged by size:
2337 2037 455
BsaHI Gr'CG_yC
Cuts at: 2260 2508 3210 4706 2260
Size: 248 702 1496 2383
Fragments arranged by size:
2383 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2018 2539 3217 3491 3638 4049 2018
Size: 521 678 274 147 411 2798
Fragments arranged by size:
2798 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 721 721
Size: 4829
BseYI C'CCAG_C
Cuts at: 2254 3012 3589 4085 2254
Size: 758 577 496 2998
Fragments arranged by size:
2998 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1816 1816
Size: 4829
BsiEI CG_ry'CG
Cuts at: 104 495 2371 2417 3625 104
Size: 391 1876 46 1208 1308
Fragments arranged by size:
1876 1308 1208 391 46
BsiHKAI G_wGCw'C
Cuts at: 1843 2621 2811 3603 1843
Size: 778 190 792 3069
Fragments arranged by size:
3069 792 778 190
BsmI GAATG_Cn'
Cuts at: 864 1261 864
Size: 397 4432
BsmBI CGTCTCn'nnnn_
Cuts at: 874 874
Size: 4829
Bsp1286I G_dGCh'C
Cuts at: 116 1843 2454 2547 2621 2811 2873 3603 116
Size: 1727 611 93 74 190 62 730 1342
Fragments arranged by size:
1727 1342 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4049 4049
Size: 4829
BspHI T'CATG_A
Cuts at: 4005 4005
Size: 4829
BspMI ACCTGCnnnn'nnnn_
Cuts at: 164 934 1879 2093 2395 2776 164
Size: 770 945 214 302 381 2217
Fragments arranged by size:
2217 945 770 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1730 3121 3175 4659 4824 50
Size: 1680 1391 54 1484 165 55
Fragments arranged by size:
1680 1484 1391 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2741 2741
Size: 4829
BsrFI r'CCGG_y
Cuts at: 2827 3008 3217 2827
Size: 181 209 4439
Fragments arranged by size:
4439 209 181
BssSI C'ACGA_G
Cuts at: 3100 3458 3100
Size: 358 4471
BstAPI GCAn_nnn'nTGC
Cuts at: 824 934 1396 2206 824
Size: 110 462 810 3447
Fragments arranged by size:
3447 810 462 110
BstBI TT'CG_AA
Cuts at: 963 3190 963
Size: 2227 2602
BstXI CCAn_nnnn'nTGG
Cuts at: 1012 1012
Size: 4829
BstYI r'GATC_y
Cuts at: 1 263 336 720 1854 2321 2344 2679 2925
Size: 262 73 384 1134 467 23 335 246
Cuts at: 2925 3937 4023 4035 4381 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1134 1012 467 449 384 346 335 262
246 86 73 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4829
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2779 2960 3123 2779
Size: 181 163 4485
Fragments arranged by size:
4485 181 163
BtsI GCAGTG_nn'
Cuts at: 1369 2072 1369
Size: 703 4126
DraI TTT'AAA
Cuts at: 1828 1828
Size: 4829
DrdI GACnn_nn'nnGTC
Cuts at: 2535 3393 2535
Size: 858 3971
EaeI y'GGCC_r
Cuts at: 526 1455 2414 2588 2979 3006 4351 526
Size: 929 959 174 391 27 1345 1004
Fragments arranged by size:
1345 1004 959 929 391 174 27
EagI C'GGCC_G
Cuts at: 2414 2414
Size: 4829
EarI CTCTTCn'nnn_
Cuts at: 200 744 965 1433 1586 1784 2852 3062 200
Size: 544 221 468 153 198 1068 210 1967
Fragments arranged by size:
1967 1068 544 468 221 210 198 153
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3037 3347 3493 4639 3037
Size: 310 146 1146 3227
Fragments arranged by size:
3227 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1841 1841
Size: 4829
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 494 1034 1505 1808 2654 3086 3833 4101 494
Size: 540 471 303 846 432 747 268 1222
Fragments arranged by size:
1222 846 747 540 471 432 303 268
EcoNI CCTnn'n_nnAGG
Cuts at: 181 1457 181
Size: 1276 3553
EcoO109I rG'GnC_Cy
Cuts at: 1968 1968
Size: 4829
EcoRI G'AATT_C
Cuts at: 787 1833 787
Size: 1046 3783
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2848 2848
Size: 4829
FspI TGC'GCA
Cuts at: 2610 2610
Size: 4829
HaeII r_GCGC'y
Cuts at: 2263 2511 3533 4512 2263
Size: 248 1022 979 2580
Fragments arranged by size:
2580 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 645 677 1434 1466 2671 2701 2703 2733 645
Size: 32 757 32 1205 30 2 30 2741
Fragments arranged by size:
2741 1205 757 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1818 1868 1818
Size: 50 4779
KasI G'GCGC_C
Cuts at: 2259 2507 2259
Size: 248 4581
KpnI G_GTAC'C
Cuts at: 1849 1849
Size: 4829
MfeI C'AATT_G
Cuts at: 217 1250 217
Size: 1033 3796
MluI A'CGCG_T
Cuts at: 4262 4262
Size: 4829
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1357 3500 3684 1357
Size: 2143 184 2502
Fragments arranged by size:
2502 2143 184
MscI TGG'CCA
Cuts at: 528 1457 2590 528
Size: 929 1133 2767
Fragments arranged by size:
2767 1133 929
MslI CAynn'nnrTG
Cuts at: 553 616 1178 2818 2945 553
Size: 63 562 1640 127 2437
Fragments arranged by size:
2437 1640 562 127 63
MspA1I CmG'CkG
Cuts at: 1940 2254 2614 3627 3872 1940
Size: 314 360 1013 245 2897
Fragments arranged by size:
2897 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3010 3010
Size: 4829
NarI GG'CG_CC
Cuts at: 2260 2508 2260
Size: 248 4581
NcoI C'CATG_G
Cuts at: 107 107
Size: 4829
NgoMIV G'CCGG_C
Cuts at: 3008 3008
Size: 4829
NsiI A_TGCA'T
Cuts at: 525 864 1671 525
Size: 339 807 3683
Fragments arranged by size:
3683 807 339
NspI r_CATG'y
Cuts at: 1882 2382 3289 1882
Size: 500 907 3422
Fragments arranged by size:
3422 907 500
PciI A'CATG_T
Cuts at: 3285 3285
Size: 4829
PmeI GTTT'AAAC
Cuts at: 1828 1828
Size: 4829
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1262 1294 3968 4000 1262
Size: 32 2674 32 2091
Fragments arranged by size:
2674 2091 32 32
PstI C_TGCA'G
Cuts at: 1359 1876 2561 1359
Size: 517 685 3627
Fragments arranged by size:
3627 685 517
PvuI CG_AT'CG
Cuts at: 104 495 104
Size: 391 4438
PvuII CAG'CTG
Cuts at: 2254 2614 2254
Size: 360 4469
RsrII CG'GwC_CG
Cuts at: 3024 3024
Size: 4829
SacI G_AGCT'C
Cuts at: 1843 1843
Size: 4829
SalI G'TCGA_C
Cuts at: 1866 1866
Size: 4829
SapI GCTCTTCn'nnn_
Cuts at: 965 2852 3062 965
Size: 1887 210 2732
Fragments arranged by size:
2732 1887 210
SbfI CC_TGCA'GG
Cuts at: 1876 1876
Size: 4829
ScaI AGT'ACT
Cuts at: 1746 4346 1746
Size: 2600 2229
SexAI A'CCwGG_T
Cuts at: 1820 1820
Size: 4829
SfcI C'TryA_G
Cuts at: 33 1355 1872 2557 3550 3741 33
Size: 1322 517 685 993 191 1121
Fragments arranged by size:
1322 1121 993 685 517 191
SfoI GGC'GCC
Cuts at: 2261 2509 2261
Size: 248 4581
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4829
SmaI CCC'GGG
Cuts at: 1851 1851
Size: 4829
SmlI C'TyrA_G
Cuts at: 662 1984 2899 3391 3653 662
Size: 1322 915 492 262 1838
Fragments arranged by size:
1838 1322 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4829
SphI G_CATG'C
Cuts at: 1882 2382 1882
Size: 500 4329
SspI AAT'ATT
Cuts at: 1536 1536
Size: 4829
StyI C'CwwG_G
Cuts at: 107 591 1005 1026 1055 1379 1458 1963 2317
Size: 484 414 21 29 324 79 505 354
Cuts at: 2317 107
Size: 2619
Fragments arranged by size:
2619 505 484 414 354 324 79 29
21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 154 2412 2545 3004 3213 154
Size: 2258 133 459 209 1770
Fragments arranged by size:
2258 1770 459 209 133
TatI w'GTAC_w
Cuts at: 1744 4344 1744
Size: 2600 2229
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1680 1712 4346 4378 1680
Size: 32 2634 32 2131
Fragments arranged by size:
2634 2131 32 32
Tth111I GACn'n_nGTC
Cuts at: 2626 2626
Size: 4829
XbaI T'CTAG_A
Cuts at: 1860 1860
Size: 4829
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 426 2324 426
Size: 1898 2931
XmaI C'CCGG_G
Cuts at: 1849 1849
Size: 4829
XmnI GAAnn'nnTTC
Cuts at: 1274 1274
Size: 4829
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbvCI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI BaeI BbsI BcgI BglI BmtI BsaXI BsiWI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI NdeI NheI NotI
NruI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI