Restriction map for
pF1KB3240
(Circular) (Six-base) MAPSORT of: pF1KB3240.seq Check: 4786 from: 1 to: 5732
pF1KB3240 5732 bp
With 182 enzymes: SgfI *
January 9, 2009 11:10 ..
AccI GT'mk_AC
Cuts at: 2770 2770
Size: 5732
Acc65I G'GTAC_C
Cuts at: 1398 2748 1398
Size: 1350 4382
AclI AA'CG_TT
Cuts at: 1113 5572 1113
Size: 4459 1273
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 749 773 1840 2497 3557 3989 4342 5004 749
Size: 24 1067 657 1060 432 353 662 1477
Fragments arranged by size:
1477 1067 1060 662 657 432 353 24
AflII C'TTAA_G
Cuts at: 2131 2461 2131
Size: 330 5402
AflIII A'CryG_T
Cuts at: 2694 3084 4884 5165 2694
Size: 390 1800 281 3261
Fragments arranged by size:
3261 1800 390 281
AleI CACnn'nnGTG
Cuts at: 856 856
Size: 5732
AlwNI CAG_nnn'CTG
Cuts at: 796 3037 4475 796
Size: 2241 1438 2053
Fragments arranged by size:
2241 2053 1438
ApaLI G'TGCA_C
Cuts at: 2069 4570 2069
Size: 2501 3231
ApoI r'AATT_y
Cuts at: 402 495 921 1668 2437 2736 5085 5341 402
Size: 93 426 747 769 299 2349 256 793
Fragments arranged by size:
2349 793 769 747 426 299 256 93
AseI AT'TA_AT
Cuts at: 20 20
Size: 5732
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5732
AvaI C'yCGr_G
Cuts at: 1141 2752 1141
Size: 1611 4121
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 890 923 890
Size: 33 5699
BamHI G'GATC_C
Cuts at: 2757 2757
Size: 5732
BanI G'GyrC_C
Cuts at: 1398 2748 3162 3410 3445 1398
Size: 1350 414 248 35 3685
Fragments arranged by size:
3685 1350 414 248 35
BanII G_rGCy'C
Cuts at: 2746 3776 2746
Size: 1030 4702
BbeI G_GCGC'C
Cuts at: 3166 3414 3166
Size: 248 5484
BbsI GAAGACnn'nnnn_
Cuts at: 2312 2376 2312
Size: 64 5668
BciVI GTATCCnnnnn_n'
Cuts at: 3630 4686 5732 3630
Size: 1056 1046 3630
Fragments arranged by size:
3630 1056 1046
BclI T'GATC_A
Cuts at: 3252 3252
Size: 5732
BglII A'GATC_T
Cuts at: 348 3247 5284 348
Size: 2899 2037 796
Fragments arranged by size:
2899 2037 796
BlpI GC'TnA_GC
Cuts at: 914 1409 2844 914
Size: 495 1435 3802
Fragments arranged by size:
3802 1435 495
Bme1580I G_kGCm'C
Cuts at: 1917 2073 3357 3450 4574 1917
Size: 156 1284 93 1124 3075
Fragments arranged by size:
3075 1284 1124 156 93
BmrI ACTGGGnnnn_n'
Cuts at: 2421 2944 3025 3360 5504 2421
Size: 523 81 335 2144 2649
Fragments arranged by size:
2649 2144 523 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 261 4099 261
Size: 3838 1894
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 329 1716 2060 329
Size: 1387 344 4001
Fragments arranged by size:
4001 1387 344
Bpu10I CC'TnA_GC
Cuts at: 1778 2114 1778
Size: 336 5396
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 231 1049 1289 2908 3787 4501 4799 193
Size: 38 818 240 1619 879 714 298 1126
Fragments arranged by size:
1619 1126 879 818 714 298 240 38
BsaI GGTCTCn'nnnn_
Cuts at: 2340 5436 2340
Size: 3096 2636
BsaAI yAC'GTr
Cuts at: 3085 3085
Size: 5732
BsaBI GATnn'nnATC
Cuts at: 6 992 3246 5283 6
Size: 986 2254 2037 455
Fragments arranged by size:
2254 2037 986 455
BsaHI Gr'CG_yC
Cuts at: 3163 3411 4113 5609 3163
Size: 248 702 1496 3286
Fragments arranged by size:
3286 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2147 2921 3442 4120 4531 4678 4952 2147
Size: 774 521 678 411 147 274 2927
Fragments arranged by size:
2927 774 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1230 1260 1230
Size: 30 5702
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 601 1168 2629 601
Size: 567 1461 3704
Fragments arranged by size:
3704 1461 567
BseYI C'CCAG_C
Cuts at: 3157 3915 4580 4988 3157
Size: 758 665 408 3901
Fragments arranged by size:
3901 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1754 2107 1754
Size: 353 5379
BsiEI CG_ry'CG
Cuts at: 104 3274 3320 4550 104
Size: 3170 46 1230 1286
Fragments arranged by size:
3170 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2073 2746 3524 3714 4574 2073
Size: 673 778 190 860 3231
Fragments arranged by size:
3231 860 778 673 190
BsmI GAATG_Cn'
Cuts at: 430 597 962 1505 430
Size: 167 365 543 4657
Fragments arranged by size:
4657 543 365 167
Bsp1286I G_dGCh'C
Cuts at: 1917 2073 2746 3357 3450 3524 3714 3776 4574
Size: 156 673 611 93 74 190 62 798
Cuts at: 4574 1917
Size: 3075
Fragments arranged by size:
3075 798 673 611 190 156 93 74
62
BspEI T'CCGG_A
Cuts at: 2147 2147
Size: 5732
BspHI T'CATG_A
Cuts at: 264 656 4164 264
Size: 392 3508 1832
Fragments arranged by size:
3508 1832 392
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1410 2008 2535 2782 2996 3298 3679 1410
Size: 598 527 247 214 302 381 3463
Fragments arranged by size:
3463 598 527 381 302 247 214
BsrBI CCG'CTC
Cuts at: 50 4024 4078 5562 5727 50
Size: 3974 54 1484 165 55
Fragments arranged by size:
3974 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3644 3644
Size: 5732
BsrFI r'CCGG_y
Cuts at: 3730 3911 3730
Size: 181 5551
BssSI C'ACGA_G
Cuts at: 4003 4711 4003
Size: 708 5024
BstAPI GCAn_nnn'nTGC
Cuts at: 3109 3109
Size: 5732
BstBI TT'CG_AA
Cuts at: 4093 4093
Size: 5732
BstXI CCAn_nnnn'nTGG
Cuts at: 1279 1279
Size: 5732
BstYI r'GATC_y
Cuts at: 1 162 348 2757 3224 3247 3582 3828 4134
Size: 161 186 2409 467 23 335 246 306
Cuts at: 4134 4146 4232 5284 1
Size: 12 86 1052 449
Fragments arranged by size:
2409 1052 467 449 335 306 246 186
161 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2326 2326
Size: 5732
BtgI C'CryG_G
Cuts at: 107 1745 107
Size: 1638 4094
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3682 3863 4026 3682
Size: 181 163 5388
Fragments arranged by size:
5388 181 163
BtsI GCAGTG_nn'
Cuts at: 2975 2975
Size: 5732
ClaI AT'CG_AT
Cuts at: 1943 1943
Size: 5732
DraI TTT'AAA
Cuts at: 1118 1824 1982 2442 2731 1118
Size: 706 158 460 289 4119
Fragments arranged by size:
4119 706 460 289 158
DrdI GACnn_nn'nnGTC
Cuts at: 3438 4782 3438
Size: 1344 4388
EaeI y'GGCC_r
Cuts at: 2158 2186 3317 3491 3882 3909 5254 2158
Size: 28 1131 174 391 27 1345 2636
Fragments arranged by size:
2636 1345 1131 391 174 28 27
EagI C'GGCC_G
Cuts at: 3317 3317
Size: 5732
EarI CTCTTCn'nnn_
Cuts at: 251 485 725 1929 1982 2165 2358 2639 3755
Size: 234 240 1204 53 183 193 281 1116
Cuts at: 3755 3965 251
Size: 210 2018
Fragments arranged by size:
2018 1204 1116 281 240 234 210 193
183 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3940 4682 4828 5542 3940
Size: 742 146 714 4130
Fragments arranged by size:
4130 742 714 146
EcoICRI GAG'CTC
Cuts at: 2744 2744
Size: 5732
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 329 749 773 1716 1840 2060 2497 3557 3989
Size: 420 24 943 124 220 437 1060 432
Cuts at: 3989 4342 5004 329
Size: 353 662 1057
Fragments arranged by size:
1060 1057 943 662 437 432 420 353
220 124 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2078 2078
Size: 5732
EcoO109I rG'GnC_Cy
Cuts at: 199 2871 199
Size: 2672 3060
EcoRI G'AATT_C
Cuts at: 921 2736 921
Size: 1815 3917
EcoRV GAT'ATC
Cuts at: 182 182
Size: 5732
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3751 3751
Size: 5732
FspI TGC'GCA
Cuts at: 3513 3513
Size: 5732
HaeII r_GCGC'y
Cuts at: 2294 3166 3414 4644 5415 2294
Size: 872 248 1230 771 2611
Fragments arranged by size:
2611 1230 872 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 166 198 2414 2446 3574 3604 3606 3636 166
Size: 32 2216 32 1128 30 2 30 2262
Fragments arranged by size:
2262 2216 1128 32 32 30 30 2
HincII GTy'rAC
Cuts at: 506 2520 2771 506
Size: 2014 251 3467
Fragments arranged by size:
3467 2014 251
HindIII A'AGCT_T
Cuts at: 1976 1976
Size: 5732
HpaI GTT'AAC
Cuts at: 2520 2520
Size: 5732
KasI G'GCGC_C
Cuts at: 3162 3410 3162
Size: 248 5484
KpnI G_GTAC'C
Cuts at: 1402 2752 1402
Size: 1350 4382
MluI A'CGCG_T
Cuts at: 5165 5165
Size: 5732
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1114 1474 4491 4675 1114
Size: 360 3017 184 2171
Fragments arranged by size:
3017 2171 360 184
MscI TGG'CCA
Cuts at: 2160 2188 3493 2160
Size: 28 1305 4399
Fragments arranged by size:
4399 1305 28
MslI CAynn'nnrTG
Cuts at: 389 661 856 3721 3848 389
Size: 272 195 2865 127 2273
Fragments arranged by size:
2865 2273 272 195 127
MspA1I CmG'CkG
Cuts at: 2121 2843 3157 3517 4301 4546 2121
Size: 722 314 360 784 245 3307
Fragments arranged by size:
3307 784 722 360 314 245
NaeI GCC'GGC
Cuts at: 3913 3913
Size: 5732
NarI GG'CG_CC
Cuts at: 3163 3411 3163
Size: 248 5484
NcoI C'CATG_G
Cuts at: 107 107
Size: 5732
NdeI CA'TA_TG
Cuts at: 2531 2531
Size: 5732
NgoMIV G'CCGG_C
Cuts at: 3911 3911
Size: 5732
NspI r_CATG'y
Cuts at: 1271 2130 2698 2785 3285 4888 1271
Size: 859 568 87 500 1603 2115
Fragments arranged by size:
2115 1603 859 568 500 87
PciI A'CATG_T
Cuts at: 2694 4884 2694
Size: 2190 3542
PflMI CCAn_nnn'nTGG
Cuts at: 1762 1762
Size: 5732
PfoI T'CCnGG_A
Cuts at: 879 1786 879
Size: 907 4825
PmeI GTTT'AAAC
Cuts at: 2731 2731
Size: 5732
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4178 4210 4178
Size: 32 5700
PpuMI rG'GwC_Cy
Cuts at: 199 199
Size: 5732
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1173 1205 1173
Size: 32 5700
PstI C_TGCA'G
Cuts at: 1422 1839 2058 2779 3464 1422
Size: 417 219 721 685 3690
Fragments arranged by size:
3690 721 685 417 219
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5732
PvuII CAG'CTG
Cuts at: 2121 3157 3517 2121
Size: 1036 360 4336
Fragments arranged by size:
4336 1036 360
RsrII CG'GwC_CG
Cuts at: 3927 3927
Size: 5732
SacI G_AGCT'C
Cuts at: 2746 2746
Size: 5732
SalI G'TCGA_C
Cuts at: 2769 2769
Size: 5732
SapI GCTCTTCn'nnn_
Cuts at: 3755 3965 3755
Size: 210 5522
SbfI CC_TGCA'GG
Cuts at: 2779 2779
Size: 5732
ScaI AGT'ACT
Cuts at: 813 843 5249 813
Size: 30 4406 1296
Fragments arranged by size:
4406 1296 30
SfcI C'TryA_G
Cuts at: 33 218 611 1343 1418 1835 2054 2775 3460
Size: 185 393 732 75 417 219 721 685
Cuts at: 3460 4428 4619 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 732 721 685 417 393 219
191 185 75
SfoI GGC'GCC
Cuts at: 3164 3412 3164
Size: 248 5484
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5732
SmaI CCC'GGG
Cuts at: 2754 2754
Size: 5732
SmlI C'TyrA_G
Cuts at: 172 246 1064 1304 2131 2461 2887 3802 4516
Size: 74 818 240 827 330 426 915 714
Cuts at: 4516 4778 172
Size: 262 1126
Fragments arranged by size:
1126 915 827 818 714 426 330 262
240 74
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5732
SphI G_CATG'C
Cuts at: 2785 3285 2785
Size: 500 5232
SspI AAT'ATT
Cuts at: 328 328
Size: 5732
StuI AGG'CCT
Cuts at: 1613 1613
Size: 5732
StyI C'CwwG_G
Cuts at: 107 2866 3220 107
Size: 2759 354 2619
Fragments arranged by size:
2759 2619 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 497 3315 3448 3907 4116 497
Size: 2818 133 459 209 2113
Fragments arranged by size:
2818 2113 459 209 133
TatI w'GTAC_w
Cuts at: 331 811 841 1881 2086 5247 331
Size: 480 30 1040 205 3161 816
Fragments arranged by size:
3161 1040 816 480 205 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5249 5281 5249
Size: 32 5700
Tth111I GACn'n_nGTC
Cuts at: 3529 3529
Size: 5732
XbaI T'CTAG_A
Cuts at: 2763 2763
Size: 5732
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3227 3227
Size: 5732
XmaI C'CCGG_G
Cuts at: 2752 2752
Size: 5732
XmnI GAAnn'nnTTC
Cuts at: 260 1171 260
Size: 911 4821
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AlfI AloI ApaI
AscI AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I CspCI DraIII
FseI FspAI MfeI NheI NotI NruI NsiI PacI
PasI PmlI PshAI PsiI PspOMI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI