Restriction map for
pF1KB3241
(Circular) (Six-base) MAPSORT of: pF1KB3241.seq Check: 3504 from: 1 to: 5990
pF1KB3241 5990 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 2966 3028 2966
Size: 62 5928
Acc65I G'GTAC_C
Cuts at: 3006 3006
Size: 5990
AclI AA'CG_TT
Cuts at: 5830 5830
Size: 5990
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 409 946 1411 1703 1784 3815 4247 4600 5262
Size: 537 465 292 81 2031 432 353 662
Cuts at: 5262 409
Size: 1137
Fragments arranged by size:
2031 1137 662 537 465 432 353 292
81
AflIII A'CryG_T
Cuts at: 3342 5142 5423 3342
Size: 1800 281 3909
Fragments arranged by size:
3909 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 523 523
Size: 5990
AleI CACnn'nnGTG
Cuts at: 1988 1988
Size: 5990
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 958 958
Size: 5990
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2613 2645 2613
Size: 32 5958
AlwNI CAG_nnn'CTG
Cuts at: 1461 1654 3295 4733 1461
Size: 193 1641 1438 2718
Fragments arranged by size:
2718 1641 1438 193
ApaLI G'TGCA_C
Cuts at: 828 4828 828
Size: 4000 1990
ApoI r'AATT_y
Cuts at: 948 1240 1312 2241 2496 2772 2869 2994 5343
Size: 292 72 929 255 276 97 125 2349
Cuts at: 5343 5599 948
Size: 256 1339
Fragments arranged by size:
2349 1339 929 292 276 256 255 125
97 72
AseI AT'TA_AT
Cuts at: 20 20
Size: 5990
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5990
AvaI C'yCGr_G
Cuts at: 3010 3010
Size: 5990
BamHI G'GATC_C
Cuts at: 3015 3015
Size: 5990
BanI G'GyrC_C
Cuts at: 468 1133 3006 3420 3668 3703 468
Size: 665 1873 414 248 35 2755
Fragments arranged by size:
2755 1873 665 414 248 35
BanII G_rGCy'C
Cuts at: 254 2015 2687 3004 4034 254
Size: 1761 672 317 1030 2210
Fragments arranged by size:
2210 1761 1030 672 317
BbeI G_GCGC'C
Cuts at: 1137 3424 3672 1137
Size: 2287 248 3455
Fragments arranged by size:
3455 2287 248
BbsI GAAGACnn'nnnn_
Cuts at: 1772 2171 1772
Size: 399 5591
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2043 2077 2043
Size: 34 5956
BciVI GTATCCnnnnn_n'
Cuts at: 2080 3888 4944 5990 2080
Size: 1808 1056 1046 2080
Fragments arranged by size:
2080 1808 1056 1046
BclI T'GATC_A
Cuts at: 1536 3510 1536
Size: 1974 4016
BfrBI ATG'CAT
Cuts at: 619 2156 619
Size: 1537 4453
BglII A'GATC_T
Cuts at: 897 2712 2821 3505 5542 897
Size: 1815 109 684 2037 1345
Fragments arranged by size:
2037 1815 1345 684 109
BlpI GC'TnA_GC
Cuts at: 3102 3102
Size: 5990
Bme1580I G_kGCm'C
Cuts at: 832 3615 3708 4832 832
Size: 2783 93 1124 1990
Fragments arranged by size:
2783 1990 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 2125 2940 3202 3283 3618 5762 2125
Size: 815 262 81 335 2144 2353
Fragments arranged by size:
2353 2144 815 335 262 81
BmtI G_CTAG'C
Cuts at: 1150 1150
Size: 5990
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4357 4357
Size: 5990
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 2800 244
Size: 2556 3434
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1926 3166 4045 4759 5057 1926
Size: 1240 879 714 298 2859
Fragments arranged by size:
2859 1240 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 630 2616 5694 630
Size: 1986 3078 926
Fragments arranged by size:
3078 1986 926
BsaAI yAC'GTr
Cuts at: 3343 3343
Size: 5990
BsaBI GATnn'nnATC
Cuts at: 6 2299 2894 3504 5541 6
Size: 2293 595 610 2037 455
Fragments arranged by size:
2293 2037 610 595 455
BsaHI Gr'CG_yC
Cuts at: 1134 1845 2732 3421 3669 4371 5867 1134
Size: 711 887 689 248 702 1496 1257
Fragments arranged by size:
1496 1257 887 711 702 689 248
BsaWI w'CCGG_w
Cuts at: 279 3179 3700 4378 4789 4936 5210 279
Size: 2900 521 678 411 147 274 1059
Fragments arranged by size:
2900 1059 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 138 168 2613 2643 138
Size: 30 2445 30 3485
Fragments arranged by size:
3485 2445 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 165 1678 165
Size: 1513 4477
BseYI C'CCAG_C
Cuts at: 314 3415 4173 4838 5246 314
Size: 3101 758 665 408 1058
Fragments arranged by size:
3101 1058 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 911 1250 911
Size: 339 5651
BsiEI CG_ry'CG
Cuts at: 104 3532 3578 4808 104
Size: 3428 46 1230 1286
Fragments arranged by size:
3428 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 578 832 1688 2015 2687 3004 3782 3972 4832
Size: 254 856 327 672 317 778 190 860
Cuts at: 4832 578
Size: 1736
Fragments arranged by size:
1736 860 856 778 672 327 317 254
190
BsmI GAATG_Cn'
Cuts at: 1206 1338 1488 2158 1206
Size: 132 150 670 5038
Fragments arranged by size:
5038 670 150 132
BsmBI CGTCTCn'nnnn_
Cuts at: 2823 2823
Size: 5990
Bsp1286I G_dGCh'C
Cuts at: 254 578 832 1688 2015 2687 3004 3615 3708
Size: 324 254 856 327 672 317 611 93
Cuts at: 3708 3782 3972 4034 4832 254
Size: 74 190 62 798 1412
Fragments arranged by size:
1412 856 798 672 611 327 324 317
254 190 93 74 62
BspHI T'CATG_A
Cuts at: 4422 4422
Size: 5990
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3040 3254 3556 3937 3040
Size: 214 302 381 5093
Fragments arranged by size:
5093 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1968 4282 4336 5820 5985 50
Size: 1918 2314 54 1484 165 55
Fragments arranged by size:
2314 1918 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 906 3902 906
Size: 2996 2994
BsrFI r'CCGG_y
Cuts at: 1136 2555 3988 4169 1136
Size: 1419 1433 181 2957
Fragments arranged by size:
2957 1433 1419 181
BssSI C'ACGA_G
Cuts at: 4261 4969 4261
Size: 708 5282
BstAPI GCAn_nnn'nTGC
Cuts at: 2456 3367 2456
Size: 911 5079
BstBI TT'CG_AA
Cuts at: 2940 4351 2940
Size: 1411 4579
BstYI r'GATC_y
Cuts at: 1 897 2697 2712 2821 3015 3482 3505 3840
Size: 896 1800 15 109 194 467 23 335
Cuts at: 3840 4086 4392 4404 4490 5542 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1800 1052 896 467 449 335 306 246
194 109 86 23 15 12
BtgI C'CryG_G
Cuts at: 498 784 498
Size: 286 5704
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3940 4121 4284 3940
Size: 181 163 5646
Fragments arranged by size:
5646 181 163
BtsI GCAGTG_nn'
Cuts at: 1163 2457 3233 1163
Size: 1294 776 3920
Fragments arranged by size:
3920 1294 776
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 905 940 905
Size: 35 5955
DraI TTT'AAA
Cuts at: 410 2877 2989 410
Size: 2467 112 3411
Fragments arranged by size:
3411 2467 112
DrdI GACnn_nn'nnGTC
Cuts at: 1036 3696 5040 1036
Size: 2660 1344 1986
Fragments arranged by size:
2660 1986 1344
EaeI y'GGCC_r
Cuts at: 837 2743 3575 3749 4140 4167 5512 837
Size: 1906 832 174 391 27 1345 1315
Fragments arranged by size:
1906 1345 1315 832 391 174 27
EagI C'GGCC_G
Cuts at: 3575 3575
Size: 5990
EarI CTCTTCn'nnn_
Cuts at: 183 566 869 4013 4223 183
Size: 383 303 3144 210 1950
Fragments arranged by size:
3144 1950 383 303 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4198 4940 5086 5800 4198
Size: 742 146 714 4388
Fragments arranged by size:
4388 742 714 146
EcoICRI GAG'CTC
Cuts at: 2013 2685 3002 2013
Size: 672 317 5001
Fragments arranged by size:
5001 672 317
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 409 946 1411 1703 1784 2800 3815 4247
Size: 165 537 465 292 81 1016 1015 432
Cuts at: 4247 4600 5262 244
Size: 353 662 972
Fragments arranged by size:
1016 1015 972 662 537 465 432 353
292 165 81
EcoNI CCTnn'n_nnAGG
Cuts at: 192 298 410 1990 2020 192
Size: 106 112 1580 30 4162
Fragments arranged by size:
4162 1580 112 106 30
EcoO109I rG'GnC_Cy
Cuts at: 1829 2946 3129 1829
Size: 1117 183 4690
Fragments arranged by size:
4690 1117 183
EcoRI G'AATT_C
Cuts at: 2496 2994 2496
Size: 498 5492
EcoRV GAT'ATC
Cuts at: 2262 2262
Size: 5990
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2734 4009 2734
Size: 1275 4715
FspI TGC'GCA
Cuts at: 3771 3771
Size: 5990
HaeII r_GCGC'y
Cuts at: 1137 3424 3672 4902 5673 1137
Size: 2287 248 1230 771 1454
Fragments arranged by size:
2287 1454 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 509 541 1714 1744 1746 1776 3832 3862 3864
Size: 32 1173 30 2 30 2056 30 2
Cuts at: 3864 3894 509
Size: 30 2605
Fragments arranged by size:
2605 2056 1173 32 30 30 30 30
2 2
HincII GTy'rAC
Cuts at: 3029 3029
Size: 5990
KasI G'GCGC_C
Cuts at: 1133 3420 3668 1133
Size: 2287 248 3455
Fragments arranged by size:
3455 2287 248
KpnI G_GTAC'C
Cuts at: 3010 3010
Size: 5990
MfeI C'AATT_G
Cuts at: 268 268
Size: 5990
MluI A'CGCG_T
Cuts at: 5423 5423
Size: 5990
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 181 1238 1476 2292 4749 4933 181
Size: 1057 238 816 2457 184 1238
Fragments arranged by size:
2457 1238 1057 816 238 184
MscI TGG'CCA
Cuts at: 839 3751 839
Size: 2912 3078
MslI CAynn'nnrTG
Cuts at: 167 511 1438 1988 3979 4106 167
Size: 344 927 550 1991 127 2051
Fragments arranged by size:
2051 1991 927 550 344 127
MspA1I CmG'CkG
Cuts at: 314 721 736 3101 3415 3775 4559 4804 314
Size: 407 15 2365 314 360 784 245 1500
Fragments arranged by size:
2365 1500 784 407 360 314 245 15
NaeI GCC'GGC
Cuts at: 1138 4171 1138
Size: 3033 2957
NarI GG'CG_CC
Cuts at: 1134 3421 3669 1134
Size: 2287 248 3455
Fragments arranged by size:
3455 2287 248
NcoI C'CATG_G
Cuts at: 498 498
Size: 5990
NdeI CA'TA_TG
Cuts at: 2046 2046
Size: 5990
NgoMIV G'CCGG_C
Cuts at: 1136 4169 1136
Size: 3033 2957
NheI G'CTAG_C
Cuts at: 1146 1146
Size: 5990
NsiI A_TGCA'T
Cuts at: 621 2158 621
Size: 1537 4453
NspI r_CATG'y
Cuts at: 999 1856 3043 3543 5146 999
Size: 857 1187 500 1603 1843
Fragments arranged by size:
1843 1603 1187 857 500
PasI CC'CwG_GG
Cuts at: 2950 2950
Size: 5990
PciI A'CATG_T
Cuts at: 5142 5142
Size: 5990
PflMI CCAn_nnn'nTGG
Cuts at: 2137 2341 2523 2137
Size: 204 182 5604
Fragments arranged by size:
5604 204 182
PfoI T'CCnGG_A
Cuts at: 437 2015 437
Size: 1578 4412
PmeI GTTT'AAAC
Cuts at: 2989 2989
Size: 5990
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2613 2645 4436 4468 2613
Size: 32 1791 32 4135
Fragments arranged by size:
4135 1791 32 32
PpuMI rG'GwC_Cy
Cuts at: 2946 2946
Size: 5990
PstI C_TGCA'G
Cuts at: 1094 1982 2787 3037 3722 1094
Size: 888 805 250 685 3362
Fragments arranged by size:
3362 888 805 685 250
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5990
PvuII CAG'CTG
Cuts at: 314 721 736 3415 3775 314
Size: 407 15 2679 360 2529
Fragments arranged by size:
2679 2529 407 360 15
RsrII CG'GwC_CG
Cuts at: 4185 4185
Size: 5990
SacI G_AGCT'C
Cuts at: 2015 2687 3004 2015
Size: 672 317 5001
Fragments arranged by size:
5001 672 317
SalI G'TCGA_C
Cuts at: 3027 3027
Size: 5990
SapI GCTCTTCn'nnn_
Cuts at: 566 4013 4223 566
Size: 3447 210 2333
Fragments arranged by size:
3447 2333 210
SbfI CC_TGCA'GG
Cuts at: 3037 3037
Size: 5990
ScaI AGT'ACT
Cuts at: 2705 5507 2705
Size: 2802 3188
SexAI A'CCwGG_T
Cuts at: 2625 2625
Size: 5990
SfcI C'TryA_G
Cuts at: 33 1090 1373 1978 2783 3033 3718 4686 4877
Size: 1057 283 605 805 250 685 968 191
Cuts at: 4877 33
Size: 1146
Fragments arranged by size:
1146 1057 968 805 685 605 283 250
191
SfoI GGC'GCC
Cuts at: 1135 3422 3670 1135
Size: 2287 248 3455
Fragments arranged by size:
3455 2287 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5990
SmaI CCC'GGG
Cuts at: 3012 3012
Size: 5990
SmlI C'TyrA_G
Cuts at: 1905 3145 4060 4774 5036 1905
Size: 1240 915 714 262 2859
Fragments arranged by size:
2859 1240 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5990
SphI G_CATG'C
Cuts at: 1856 3043 3543 1856
Size: 1187 500 4303
Fragments arranged by size:
4303 1187 500
SspI AAT'ATT
Cuts at: 1557 1791 1557
Size: 234 5756
StyI C'CwwG_G
Cuts at: 299 498 2136 3124 3478 299
Size: 199 1638 988 354 2811
Fragments arranged by size:
2811 1638 988 354 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2226 3573 3706 4165 4374 2226
Size: 1347 133 459 209 3842
Fragments arranged by size:
3842 1347 459 209 133
TatI w'GTAC_w
Cuts at: 2654 2703 5505 2654
Size: 49 2802 3139
Fragments arranged by size:
3139 2802 49
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5507 5539 5507
Size: 32 5958
Tth111I GACn'n_nGTC
Cuts at: 3787 3787
Size: 5990
XbaI T'CTAG_A
Cuts at: 3021 3021
Size: 5990
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3485 3485
Size: 5990
XmaI C'CCGG_G
Cuts at: 3010 3010
Size: 5990
XmnI GAAnn'nnTTC
Cuts at: 959 1515 959
Size: 556 5434
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AleI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI ApaI AscI AvrII
BaeI BbvCI BglI BmgBI Bpu10I BsiWI BspEI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII FseI
FspAI HindIII HpaI NotI NruI PacI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI