Restriction map for
pF1KB3247
(Circular) (Six-base) MAPSORT of: pF1KB3247.seq Check: 4729 from: 1 to: 4196
pF1KB3247 4196 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 190 190
Size: 4196
AccI GT'mk_AC
Cuts at: 1234 1234
Size: 4196
Acc65I G'GTAC_C
Cuts at: 1212 1212
Size: 4196
AclI AA'CG_TT
Cuts at: 4036 4036
Size: 4196
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 455 503 623 659 2021 2453 2806 3468 455
Size: 48 120 36 1362 432 353 662 1183
Fragments arranged by size:
1362 1183 662 432 353 120 48 36
AflIII A'CryG_T
Cuts at: 1548 3348 3629 1548
Size: 1800 281 2115
Fragments arranged by size:
2115 1800 281
AleI CACnn'nnGTG
Cuts at: 415 797 415
Size: 382 3814
AlwNI CAG_nnn'CTG
Cuts at: 737 1501 2939 737
Size: 764 1438 1994
Fragments arranged by size:
1994 1438 764
ApaLI G'TGCA_C
Cuts at: 3034 3034
Size: 4196
ApoI r'AATT_y
Cuts at: 902 1200 3549 3805 902
Size: 298 2349 256 1293
Fragments arranged by size:
2349 1293 298 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4196
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4196
AvaI C'yCGr_G
Cuts at: 1216 1216
Size: 4196
BamHI G'GATC_C
Cuts at: 377 1221 377
Size: 844 3352
BanI G'GyrC_C
Cuts at: 562 1212 1626 1874 1909 562
Size: 650 414 248 35 2849
Fragments arranged by size:
2849 650 414 248 35
BanII G_rGCy'C
Cuts at: 1210 2240 1210
Size: 1030 3166
BbeI G_GCGC'C
Cuts at: 1630 1878 1630
Size: 248 3948
BbsI GAAGACnn'nnnn_
Cuts at: 611 734 611
Size: 123 4073
BciVI GTATCCnnnnn_n'
Cuts at: 2094 3150 4196 2094
Size: 1056 1046 2094
Fragments arranged by size:
2094 1056 1046
BclI T'GATC_A
Cuts at: 917 1013 1716 917
Size: 96 703 3397
Fragments arranged by size:
3397 703 96
BglII A'GATC_T
Cuts at: 1711 3748 1711
Size: 2037 2159
BlpI GC'TnA_GC
Cuts at: 157 1308 157
Size: 1151 3045
Bme1580I G_kGCm'C
Cuts at: 362 1821 1914 3038 362
Size: 1459 93 1124 1520
Fragments arranged by size:
1520 1459 1124 93
BmgBI CAC'GTC
Cuts at: 639 639
Size: 4196
BmrI ACTGGGnnnn_n'
Cuts at: 438 791 1408 1489 1824 3968 438
Size: 353 617 81 335 2144 666
Fragments arranged by size:
2144 666 617 353 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2563 2563
Size: 4196
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 639 1109 639
Size: 470 3726
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1372 2251 2965 3263 1372
Size: 879 714 298 2305
Fragments arranged by size:
2305 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 325 770 3900 325
Size: 445 3130 621
Fragments arranged by size:
3130 621 445
BsaAI yAC'GTr
Cuts at: 213 363 1549 213
Size: 150 1186 2860
Fragments arranged by size:
2860 1186 150
BsaBI GATnn'nnATC
Cuts at: 6 828 1710 3747 6
Size: 822 882 2037 455
Fragments arranged by size:
2037 882 822 455
BsaHI Gr'CG_yC
Cuts at: 1627 1875 2577 4073 1627
Size: 248 702 1496 1750
Fragments arranged by size:
1750 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1385 1906 2584 2995 3142 3416 1385
Size: 521 678 411 147 274 2165
Fragments arranged by size:
2165 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 373 403 373
Size: 30 4166
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 642 642
Size: 4196
BseYI C'CCAG_C
Cuts at: 616 1621 2379 3044 3452 616
Size: 1005 758 665 408 1360
Fragments arranged by size:
1360 1005 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 419 419
Size: 4196
BsiEI CG_ry'CG
Cuts at: 104 1738 1784 3014 104
Size: 1634 46 1230 1286
Fragments arranged by size:
1634 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1210 1988 2178 3038 1210
Size: 778 190 860 2368
Fragments arranged by size:
2368 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 362 1210 1821 1914 1988 2178 2240 3038 362
Size: 848 611 93 74 190 62 798 1520
Fragments arranged by size:
1520 848 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 2628 2628
Size: 4196
BspMI ACCTGCnnnn'nnnn_
Cuts at: 190 196 329 343 518 1169 1246 1460 1762
Size: 6 133 14 175 651 77 214 302
Cuts at: 1762 2143 190
Size: 381 2243
Fragments arranged by size:
2243 651 381 302 214 175 133 77
14 6
BsrBI CCG'CTC
Cuts at: 50 2488 2542 4026 4191 50
Size: 2438 54 1484 165 55
Fragments arranged by size:
2438 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 965 1032 2108 965
Size: 67 1076 3053
Fragments arranged by size:
3053 1076 67
BsrFI r'CCGG_y
Cuts at: 2194 2375 2194
Size: 181 4015
BsrGI T'GTAC_A
Cuts at: 419 493 708 419
Size: 74 215 3907
Fragments arranged by size:
3907 215 74
BssSI C'ACGA_G
Cuts at: 148 2467 3175 148
Size: 2319 708 1169
Fragments arranged by size:
2319 1169 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1573 1573
Size: 4196
BstBI TT'CG_AA
Cuts at: 2557 2557
Size: 4196
BstXI CCAn_nnnn'nTGG
Cuts at: 799 799
Size: 4196
BstYI r'GATC_y
Cuts at: 1 377 823 1221 1688 1711 2046 2292 2598
Size: 376 446 398 467 23 335 246 306
Cuts at: 2598 2610 2696 3748 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 467 449 446 398 376 335 306
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4196
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2146 2327 2490 2146
Size: 181 163 3852
Fragments arranged by size:
3852 181 163
BtsI GCAGTG_nn'
Cuts at: 1439 1439
Size: 4196
ClaI AT'CG_AT
Cuts at: 173 173
Size: 4196
DraI TTT'AAA
Cuts at: 1195 1195
Size: 4196
DraIII CAC_nnn'GTG
Cuts at: 366 366
Size: 4196
DrdI GACnn_nn'nnGTC
Cuts at: 1902 3246 1902
Size: 1344 2852
EaeI y'GGCC_r
Cuts at: 1781 1955 2346 2373 3718 1781
Size: 174 391 27 1345 2259
Fragments arranged by size:
2259 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1781 1781
Size: 4196
EarI CTCTTCn'nnn_
Cuts at: 2219 2429 2219
Size: 210 3986
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2404 3146 3292 4006 2404
Size: 742 146 714 2594
Fragments arranged by size:
2594 742 714 146
EcoICRI GAG'CTC
Cuts at: 1208 1208
Size: 4196
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 455 503 623 639 659 1109 2021 2453 2806
Size: 48 120 16 20 450 912 432 353
Cuts at: 2806 3468 455
Size: 662 1183
Fragments arranged by size:
1183 912 662 450 432 353 120 48
20 16
EcoNI CCTnn'n_nnAGG
Cuts at: 265 265
Size: 4196
EcoO109I rG'GnC_Cy
Cuts at: 806 1335 806
Size: 529 3667
EcoRI G'AATT_C
Cuts at: 1200 1200
Size: 4196
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2215 2215
Size: 4196
FspI TGC'GCA
Cuts at: 1977 1977
Size: 4196
HaeII r_GCGC'y
Cuts at: 1630 1878 3108 3879 1630
Size: 248 1230 771 1947
Fragments arranged by size:
1947 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 131 163 481 513 671 703 826 858 2038
Size: 32 318 32 158 32 123 32 1180
Cuts at: 2038 2068 2070 2100 131
Size: 30 2 30 2227
Fragments arranged by size:
2227 1180 318 158 123 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 552 975 1235 552
Size: 423 260 3513
Fragments arranged by size:
3513 423 260
HpaI GTT'AAC
Cuts at: 552 552
Size: 4196
KasI G'GCGC_C
Cuts at: 1626 1874 1626
Size: 248 3948
KpnI G_GTAC'C
Cuts at: 1216 1216
Size: 4196
MluI A'CGCG_T
Cuts at: 3629 3629
Size: 4196
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 458 2955 3139 458
Size: 2497 184 1515
Fragments arranged by size:
2497 1515 184
MscI TGG'CCA
Cuts at: 1957 1957
Size: 4196
MslI CAynn'nnrTG
Cuts at: 415 797 845 2185 2312 415
Size: 382 48 1340 127 2299
Fragments arranged by size:
2299 1340 382 127 48
MspA1I CmG'CkG
Cuts at: 156 256 647 1144 1307 1621 1981 2765 3010
Size: 100 391 497 163 314 360 784 245
Cuts at: 3010 156
Size: 1342
Fragments arranged by size:
1342 784 497 391 360 314 245 163
100
NaeI GCC'GGC
Cuts at: 2377 2377
Size: 4196
NarI GG'CG_CC
Cuts at: 1627 1875 1627
Size: 248 3948
NcoI C'CATG_G
Cuts at: 107 107
Size: 4196
NgoMIV G'CCGG_C
Cuts at: 2375 2375
Size: 4196
NspI r_CATG'y
Cuts at: 1249 1749 3352 1249
Size: 500 1603 2093
Fragments arranged by size:
2093 1603 500
PciI A'CATG_T
Cuts at: 3348 3348
Size: 4196
PmeI GTTT'AAAC
Cuts at: 1195 1195
Size: 4196
PmlI CAC'GTG
Cuts at: 213 363 213
Size: 150 4046
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2642 2674 2642
Size: 32 4164
PpuMI rG'GwC_Cy
Cuts at: 806 806
Size: 4196
PstI C_TGCA'G
Cuts at: 340 1166 1243 1928 340
Size: 826 77 685 2608
Fragments arranged by size:
2608 826 685 77
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4196
PvuII CAG'CTG
Cuts at: 1144 1621 1981 1144
Size: 477 360 3359
Fragments arranged by size:
3359 477 360
RsrII CG'GwC_CG
Cuts at: 2391 2391
Size: 4196
SacI G_AGCT'C
Cuts at: 1210 1210
Size: 4196
SalI G'TCGA_C
Cuts at: 1233 1233
Size: 4196
SanDI GG'GwC_CC
Cuts at: 806 806
Size: 4196
SapI GCTCTTCn'nnn_
Cuts at: 2219 2429 2219
Size: 210 3986
SbfI CC_TGCA'GG
Cuts at: 340 1243 340
Size: 903 3293
ScaI AGT'ACT
Cuts at: 3713 3713
Size: 4196
SfcI C'TryA_G
Cuts at: 33 231 336 1162 1239 1924 2892 3083 33
Size: 198 105 826 77 685 968 191 1146
Fragments arranged by size:
1146 968 826 685 198 191 105 77
SfoI GGC'GCC
Cuts at: 1628 1876 1628
Size: 248 3948
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4196
SmaI CCC'GGG
Cuts at: 1218 1218
Size: 4196
SmlI C'TyrA_G
Cuts at: 1351 2266 2980 3242 1351
Size: 915 714 262 2305
Fragments arranged by size:
2305 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4196
SphI G_CATG'C
Cuts at: 1249 1749 1249
Size: 500 3696
StyI C'CwwG_G
Cuts at: 107 941 1330 1684 107
Size: 834 389 354 2619
Fragments arranged by size:
2619 834 389 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1779 1912 2371 2580 1779
Size: 133 459 209 3395
Fragments arranged by size:
3395 459 209 133
TatI w'GTAC_w
Cuts at: 419 493 708 3711 419
Size: 74 215 3003 904
Fragments arranged by size:
3003 904 215 74
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 373 405 3713 3745 373
Size: 32 3308 32 824
Fragments arranged by size:
3308 824 32 32
Tth111I GACn'n_nGTC
Cuts at: 1993 1993
Size: 4196
XbaI T'CTAG_A
Cuts at: 1227 1227
Size: 4196
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 817 1691 817
Size: 874 3322
XmaI C'CCGG_G
Cuts at: 1216 1216
Size: 4196
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PmlI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI
Bpu10I BsiWI BsmI BsmBI BspEI BssHII BstEII BstZ17I
Bsu36I CspCI EcoRV FseI FspAI HindIII MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PshAI PsiI PspOMI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI