(Circular) (Six-base) MAPSORT of: pF1KB3248.seq Check: 2115 from: 1 to: 3743 pF1KB3248 3743 bp With 182 enzymes: SgfI * June 20, 2008 10:22 .. AccI GT'mk_AC Cuts at: 781 781 Size: 3743 Acc65I G'GTAC_C Cuts at: 759 759 Size: 3743 AclI AA'CG_TT Cuts at: 3583 3583 Size: 3743 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 298 1568 2000 2353 3015 298 Size: 1270 432 353 662 1026 Fragments arranged by size: 1270 1026 662 432 353 AflIII A'CryG_T Cuts at: 1095 2895 3176 1095 Size: 1800 281 1662 Fragments arranged by size: 1800 1662 281 AlwNI CAG_nnn'CTG Cuts at: 1048 2486 1048 Size: 1438 2305 ApaLI G'TGCA_C Cuts at: 2581 2581 Size: 3743 ApoI r'AATT_y Cuts at: 229 439 451 747 3096 3352 229 Size: 210 12 296 2349 256 620 Fragments arranged by size: 2349 620 296 256 210 12 AseI AT'TA_AT Cuts at: 20 20 Size: 3743 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3743 AvaI C'yCGr_G Cuts at: 647 763 647 Size: 116 3627 BamHI G'GATC_C Cuts at: 768 768 Size: 3743 BanI G'GyrC_C Cuts at: 759 1173 1421 1456 759 Size: 414 248 35 3046 Fragments arranged by size: 3046 414 248 35 BanII G_rGCy'C Cuts at: 757 1787 757 Size: 1030 2713 BbeI G_GCGC'C Cuts at: 1177 1425 1177 Size: 248 3495 BciVI GTATCCnnnnn_n' Cuts at: 1641 2697 3743 1641 Size: 1056 1046 1641 Fragments arranged by size: 1641 1056 1046 BclI T'GATC_A Cuts at: 1263 1263 Size: 3743 BglII A'GATC_T Cuts at: 1258 3295 1258 Size: 2037 1706 BlpI GC'TnA_GC Cuts at: 855 855 Size: 3743 Bme1580I G_kGCm'C Cuts at: 1368 1461 2585 1368 Size: 93 1124 2526 Fragments arranged by size: 2526 1124 93 BmrI ACTGGGnnnn_n' Cuts at: 955 1036 1371 3515 955 Size: 81 335 2144 1183 Fragments arranged by size: 2144 1183 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2110 2110 Size: 3743 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 582 582 Size: 3743 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 919 1798 2512 2810 919 Size: 879 714 298 1852 Fragments arranged by size: 1852 879 714 298 BsaI GGTCTCn'nnnn_ Cuts at: 3447 3447 Size: 3743 BsaAI yAC'GTr Cuts at: 1096 1096 Size: 3743 BsaBI GATnn'nnATC Cuts at: 6 1257 3294 6 Size: 1251 2037 455 Fragments arranged by size: 2037 1251 455 BsaHI Gr'CG_yC Cuts at: 1174 1422 2124 3620 1174 Size: 248 702 1496 1297 Fragments arranged by size: 1496 1297 702 248 BsaWI w'CCGG_w Cuts at: 932 1453 2131 2542 2689 2963 932 Size: 521 678 411 147 274 1712 Fragments arranged by size: 1712 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 154 154 Size: 3743 BseYI C'CCAG_C Cuts at: 1168 1926 2591 2999 1168 Size: 758 665 408 1912 Fragments arranged by size: 1912 758 665 408 BsiEI CG_ry'CG Cuts at: 104 1285 1331 2561 104 Size: 1181 46 1230 1286 Fragments arranged by size: 1286 1230 1181 46 BsiHKAI G_wGCw'C Cuts at: 757 1535 1725 2585 757 Size: 778 190 860 1915 Fragments arranged by size: 1915 860 778 190 Bsp1286I G_dGCh'C Cuts at: 757 1368 1461 1535 1725 1787 2585 757 Size: 611 93 74 190 62 798 1915 Fragments arranged by size: 1915 798 611 190 93 74 62 BspHI T'CATG_A Cuts at: 225 2175 225 Size: 1950 1793 BspMI ACCTGCnnnn'nnnn_ Cuts at: 793 1007 1309 1690 793 Size: 214 302 381 2846 Fragments arranged by size: 2846 381 302 214 BsrBI CCG'CTC Cuts at: 50 2035 2089 3573 3738 50 Size: 1985 54 1484 165 55 Fragments arranged by size: 1985 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1655 1655 Size: 3743 BsrFI r'CCGG_y Cuts at: 1741 1922 1741 Size: 181 3562 BssSI C'ACGA_G Cuts at: 2014 2722 2014 Size: 708 3035 BstAPI GCAn_nnn'nTGC Cuts at: 1120 1120 Size: 3743 BstBI TT'CG_AA Cuts at: 2104 2104 Size: 3743 BstEII G'GTnAC_C Cuts at: 114 114 Size: 3743 BstYI r'GATC_y Cuts at: 1 267 768 1235 1258 1593 1839 2145 2157 Size: 266 501 467 23 335 246 306 12 Cuts at: 2157 2243 3295 1 Size: 86 1052 449 Fragments arranged by size: 1052 501 467 449 335 306 266 246 86 23 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 162 1693 1874 2037 162 Size: 1531 181 163 1868 Fragments arranged by size: 1868 1531 181 163 BtsI GCAGTG_nn' Cuts at: 986 986 Size: 3743 DraI TTT'AAA Cuts at: 742 742 Size: 3743 DrdI GACnn_nn'nnGTC Cuts at: 1449 2793 1449 Size: 1344 2399 EaeI y'GGCC_r Cuts at: 444 1328 1502 1893 1920 3265 444 Size: 884 174 391 27 1345 922 Fragments arranged by size: 1345 922 884 391 174 27 EagI C'GGCC_G Cuts at: 1328 1328 Size: 3743 EarI CTCTTCn'nnn_ Cuts at: 1766 1976 1766 Size: 210 3533 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1951 2693 2839 3553 1951 Size: 742 146 714 2141 Fragments arranged by size: 2141 742 714 146 EcoICRI GAG'CTC Cuts at: 755 755 Size: 3743 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 298 582 1568 2000 2353 3015 298 Size: 284 986 432 353 662 1026 Fragments arranged by size: 1026 986 662 432 353 284 EcoO109I rG'GnC_Cy Cuts at: 664 882 664 Size: 218 3525 EcoRI G'AATT_C Cuts at: 229 747 229 Size: 518 3225 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1762 1762 Size: 3743 FspI TGC'GCA Cuts at: 1524 1524 Size: 3743 HaeII r_GCGC'y Cuts at: 1177 1425 2655 3426 1177 Size: 248 1230 771 1494 Fragments arranged by size: 1494 1230 771 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1585 1615 1617 1647 1585 Size: 30 2 30 3681 Fragments arranged by size: 3681 30 30 2 HincII GTy'rAC Cuts at: 782 782 Size: 3743 KasI G'GCGC_C Cuts at: 1173 1421 1173 Size: 248 3495 KpnI G_GTAC'C Cuts at: 763 763 Size: 3743 MluI A'CGCG_T Cuts at: 3176 3176 Size: 3743 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2502 2686 2502 Size: 184 3559 MscI TGG'CCA Cuts at: 446 1504 446 Size: 1058 2685 MslI CAynn'nnrTG Cuts at: 112 1732 1859 112 Size: 1620 127 1996 Fragments arranged by size: 1996 1620 127 MspA1I CmG'CkG Cuts at: 854 1168 1528 2312 2557 854 Size: 314 360 784 245 2040 Fragments arranged by size: 2040 784 360 314 245 NaeI GCC'GGC Cuts at: 1924 1924 Size: 3743 NarI GG'CG_CC Cuts at: 1174 1422 1174 Size: 248 3495 NgoMIV G'CCGG_C Cuts at: 1922 1922 Size: 3743 NspI r_CATG'y Cuts at: 151 420 796 1296 2899 151 Size: 269 376 500 1603 995 Fragments arranged by size: 1603 995 500 376 269 PciI A'CATG_T Cuts at: 2895 2895 Size: 3743 PmeI GTTT'AAAC Cuts at: 742 742 Size: 3743 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2189 2221 2189 Size: 32 3711 PpuMI rG'GwC_Cy Cuts at: 664 664 Size: 3743 PspXI vC'TCGA_Gb Cuts at: 647 647 Size: 3743 PstI C_TGCA'G Cuts at: 146 309 790 1475 146 Size: 163 481 685 2414 Fragments arranged by size: 2414 685 481 163 PvuI CG_AT'CG Cuts at: 104 104 Size: 3743 PvuII CAG'CTG Cuts at: 1168 1528 1168 Size: 360 3383 RsrII CG'GwC_CG Cuts at: 1938 1938 Size: 3743 SacI G_AGCT'C Cuts at: 757 757 Size: 3743 SalI G'TCGA_C Cuts at: 780 780 Size: 3743 SanDI GG'GwC_CC Cuts at: 664 664 Size: 3743 SapI GCTCTTCn'nnn_ Cuts at: 1766 1976 1766 Size: 210 3533 SbfI CC_TGCA'GG Cuts at: 790 790 Size: 3743 ScaI AGT'ACT Cuts at: 3260 3260 Size: 3743 SfcI C'TryA_G Cuts at: 33 142 305 622 786 1471 2439 2630 33 Size: 109 163 317 164 685 968 191 1146 Fragments arranged by size: 1146 968 685 317 191 164 163 109 SfoI GGC'GCC Cuts at: 1175 1423 1175 Size: 248 3495 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3743 SmaI CCC'GGG Cuts at: 765 765 Size: 3743 SmlI C'TyrA_G Cuts at: 647 898 1813 2527 2789 647 Size: 251 915 714 262 1601 Fragments arranged by size: 1601 915 714 262 251 SpeI A'CTAG_T Cuts at: 67 67 Size: 3743 SphI G_CATG'C Cuts at: 796 1296 796 Size: 500 3243 StyI C'CwwG_G Cuts at: 877 1231 877 Size: 354 3389 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 102 1326 1459 1918 2127 102 Size: 1224 133 459 209 1718 Fragments arranged by size: 1718 1224 459 209 133 TatI w'GTAC_w Cuts at: 328 3258 328 Size: 2930 813 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 669 701 3260 3292 669 Size: 32 2559 32 1120 Fragments arranged by size: 2559 1120 32 32 Tth111I GACn'n_nGTC Cuts at: 1540 1540 Size: 3743 XbaI T'CTAG_A Cuts at: 774 774 Size: 3743 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1238 1238 Size: 3743 XhoI C'TCGA_G Cuts at: 647 647 Size: 3743 XmaI C'CCGG_G Cuts at: 763 763 Size: 3743 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI PpuMI PspXI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AgeI AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BspEI BsrGI BssHII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XmnI ZraI