Restriction map for pF1KB3248
 (Circular) (Six-base) MAPSORT of: pF1KB3248.seq  Check: 2115  from: 1  to: 3743



pF1KB3248  3743 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:22  ..

AccI GT'mk_AC

Cuts at:    781     781 
   Size:       3743
 
Acc65I G'GTAC_C

Cuts at:    759     759 
   Size:       3743
 
AclI AA'CG_TT

Cuts at:   3583    3583 
   Size:       3743
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    298    1568    2000    2353    3015     298 
   Size:       1270     432     353     662    1026

  Fragments arranged by size: 

                1270    1026     662     432     353
 
AflIII A'CryG_T

Cuts at:   1095    2895    3176    1095 
   Size:       1800     281    1662

  Fragments arranged by size: 

                1800    1662     281
 
AlwNI CAG_nnn'CTG

Cuts at:   1048    2486    1048 
   Size:       1438    2305
 
ApaLI G'TGCA_C

Cuts at:   2581    2581 
   Size:       3743
 
ApoI r'AATT_y

Cuts at:    229     439     451     747    3096    3352     229 
   Size:        210      12     296    2349     256     620

  Fragments arranged by size: 

                2349     620     296     256     210      12
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3743
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3743
 
AvaI C'yCGr_G

Cuts at:    647     763     647 
   Size:        116    3627
 
BamHI G'GATC_C

Cuts at:    768     768 
   Size:       3743
 
BanI G'GyrC_C

Cuts at:    759    1173    1421    1456     759 
   Size:        414     248      35    3046

  Fragments arranged by size: 

                3046     414     248      35
 
BanII G_rGCy'C

Cuts at:    757    1787     757 
   Size:       1030    2713
 
BbeI G_GCGC'C

Cuts at:   1177    1425    1177 
   Size:        248    3495
 
BciVI GTATCCnnnnn_n'

Cuts at:   1641    2697    3743    1641 
   Size:       1056    1046    1641

  Fragments arranged by size: 

                1641    1056    1046
 
BclI T'GATC_A

Cuts at:   1263    1263 
   Size:       3743
 
BglII A'GATC_T

Cuts at:   1258    3295    1258 
   Size:       2037    1706
 
BlpI GC'TnA_GC

Cuts at:    855     855 
   Size:       3743
 
Bme1580I G_kGCm'C

Cuts at:   1368    1461    2585    1368 
   Size:         93    1124    2526

  Fragments arranged by size: 

                2526    1124      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    955    1036    1371    3515     955 
   Size:         81     335    2144    1183

  Fragments arranged by size: 

                2144    1183     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2110    2110 
   Size:       3743
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    582     582 
   Size:       3743
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    919    1798    2512    2810     919 
   Size:        879     714     298    1852

  Fragments arranged by size: 

                1852     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3447    3447 
   Size:       3743
 
BsaAI yAC'GTr

Cuts at:   1096    1096 
   Size:       3743
 
BsaBI GATnn'nnATC

Cuts at:      6    1257    3294       6 
   Size:       1251    2037     455

  Fragments arranged by size: 

                2037    1251     455
 
BsaHI Gr'CG_yC

Cuts at:   1174    1422    2124    3620    1174 
   Size:        248     702    1496    1297

  Fragments arranged by size: 

                1496    1297     702     248
 
BsaWI w'CCGG_w

Cuts at:    932    1453    2131    2542    2689    2963     932 
   Size:        521     678     411     147     274    1712

  Fragments arranged by size: 

                1712     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    154     154 
   Size:       3743
 
BseYI C'CCAG_C

Cuts at:   1168    1926    2591    2999    1168 
   Size:        758     665     408    1912

  Fragments arranged by size: 

                1912     758     665     408
 
BsiEI CG_ry'CG

Cuts at:    104    1285    1331    2561     104 
   Size:       1181      46    1230    1286

  Fragments arranged by size: 

                1286    1230    1181      46
 
BsiHKAI G_wGCw'C

Cuts at:    757    1535    1725    2585     757 
   Size:        778     190     860    1915

  Fragments arranged by size: 

                1915     860     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    757    1368    1461    1535    1725    1787    2585     757 
   Size:        611      93      74     190      62     798    1915

  Fragments arranged by size: 

                1915     798     611     190      93      74      62
 
BspHI T'CATG_A

Cuts at:    225    2175     225 
   Size:       1950    1793
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    793    1007    1309    1690     793 
   Size:        214     302     381    2846

  Fragments arranged by size: 

                2846     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2035    2089    3573    3738      50 
   Size:       1985      54    1484     165      55

  Fragments arranged by size: 

                1985    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1655    1655 
   Size:       3743
 
BsrFI r'CCGG_y

Cuts at:   1741    1922    1741 
   Size:        181    3562
 
BssSI C'ACGA_G

Cuts at:   2014    2722    2014 
   Size:        708    3035
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1120    1120 
   Size:       3743
 
BstBI TT'CG_AA

Cuts at:   2104    2104 
   Size:       3743
 
BstEII G'GTnAC_C

Cuts at:    114     114 
   Size:       3743
 
BstYI r'GATC_y

Cuts at:      1     267     768    1235    1258    1593    1839    2145    2157 
   Size:        266     501     467      23     335     246     306      12

Cuts at:   2157    2243    3295       1 
   Size:         86    1052     449

  Fragments arranged by size: 

                1052     501     467     449     335     306     266     246
                  86      23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    162    1693    1874    2037     162 
   Size:       1531     181     163    1868

  Fragments arranged by size: 

                1868    1531     181     163
 
BtsI GCAGTG_nn'

Cuts at:    986     986 
   Size:       3743
 
DraI TTT'AAA

Cuts at:    742     742 
   Size:       3743
 
DrdI GACnn_nn'nnGTC

Cuts at:   1449    2793    1449 
   Size:       1344    2399
 
EaeI y'GGCC_r

Cuts at:    444    1328    1502    1893    1920    3265     444 
   Size:        884     174     391      27    1345     922

  Fragments arranged by size: 

                1345     922     884     391     174      27
 
EagI C'GGCC_G

Cuts at:   1328    1328 
   Size:       3743
 
EarI CTCTTCn'nnn_

Cuts at:   1766    1976    1766 
   Size:        210    3533
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1951    2693    2839    3553    1951 
   Size:        742     146     714    2141

  Fragments arranged by size: 

                2141     742     714     146
 
EcoICRI GAG'CTC

Cuts at:    755     755 
   Size:       3743
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    298     582    1568    2000    2353    3015     298 
   Size:        284     986     432     353     662    1026

  Fragments arranged by size: 

                1026     986     662     432     353     284
 
EcoO109I rG'GnC_Cy

Cuts at:    664     882     664 
   Size:        218    3525
 
EcoRI G'AATT_C

Cuts at:    229     747     229 
   Size:        518    3225
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1762    1762 
   Size:       3743
 
FspI TGC'GCA

Cuts at:   1524    1524 
   Size:       3743
 
HaeII r_GCGC'y

Cuts at:   1177    1425    2655    3426    1177 
   Size:        248    1230     771    1494

  Fragments arranged by size: 

                1494    1230     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1585    1615    1617    1647    1585 
   Size:         30       2      30    3681

  Fragments arranged by size: 

                3681      30      30       2
 
HincII GTy'rAC

Cuts at:    782     782 
   Size:       3743
 
KasI G'GCGC_C

Cuts at:   1173    1421    1173 
   Size:        248    3495
 
KpnI G_GTAC'C

Cuts at:    763     763 
   Size:       3743
 
MluI A'CGCG_T

Cuts at:   3176    3176 
   Size:       3743
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2502    2686    2502 
   Size:        184    3559
 
MscI TGG'CCA

Cuts at:    446    1504     446 
   Size:       1058    2685
 
MslI CAynn'nnrTG

Cuts at:    112    1732    1859     112 
   Size:       1620     127    1996

  Fragments arranged by size: 

                1996    1620     127
 
MspA1I CmG'CkG

Cuts at:    854    1168    1528    2312    2557     854 
   Size:        314     360     784     245    2040

  Fragments arranged by size: 

                2040     784     360     314     245
 
NaeI GCC'GGC

Cuts at:   1924    1924 
   Size:       3743
 
NarI GG'CG_CC

Cuts at:   1174    1422    1174 
   Size:        248    3495
 
NgoMIV G'CCGG_C

Cuts at:   1922    1922 
   Size:       3743
 
NspI r_CATG'y

Cuts at:    151     420     796    1296    2899     151 
   Size:        269     376     500    1603     995

  Fragments arranged by size: 

                1603     995     500     376     269
 
PciI A'CATG_T

Cuts at:   2895    2895 
   Size:       3743
 
PmeI GTTT'AAAC

Cuts at:    742     742 
   Size:       3743
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2189    2221    2189 
   Size:         32    3711
 
PpuMI rG'GwC_Cy

Cuts at:    664     664 
   Size:       3743
 
PspXI vC'TCGA_Gb

Cuts at:    647     647 
   Size:       3743
 
PstI C_TGCA'G

Cuts at:    146     309     790    1475     146 
   Size:        163     481     685    2414

  Fragments arranged by size: 

                2414     685     481     163
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3743
 
PvuII CAG'CTG

Cuts at:   1168    1528    1168 
   Size:        360    3383
 
RsrII CG'GwC_CG

Cuts at:   1938    1938 
   Size:       3743
 
SacI G_AGCT'C

Cuts at:    757     757 
   Size:       3743
 
SalI G'TCGA_C

Cuts at:    780     780 
   Size:       3743
 
SanDI GG'GwC_CC

Cuts at:    664     664 
   Size:       3743
 
SapI GCTCTTCn'nnn_

Cuts at:   1766    1976    1766 
   Size:        210    3533
 
SbfI CC_TGCA'GG

Cuts at:    790     790 
   Size:       3743
 
ScaI AGT'ACT

Cuts at:   3260    3260 
   Size:       3743
 
SfcI C'TryA_G

Cuts at:     33     142     305     622     786    1471    2439    2630      33 
   Size:        109     163     317     164     685     968     191    1146

  Fragments arranged by size: 

                1146     968     685     317     191     164     163     109
            
 
SfoI GGC'GCC

Cuts at:   1175    1423    1175 
   Size:        248    3495
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3743
 
SmaI CCC'GGG

Cuts at:    765     765 
   Size:       3743
 
SmlI C'TyrA_G

Cuts at:    647     898    1813    2527    2789     647 
   Size:        251     915     714     262    1601

  Fragments arranged by size: 

                1601     915     714     262     251
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3743
 
SphI G_CATG'C

Cuts at:    796    1296     796 
   Size:        500    3243
 
StyI C'CwwG_G

Cuts at:    877    1231     877 
   Size:        354    3389
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    102    1326    1459    1918    2127     102 
   Size:       1224     133     459     209    1718

  Fragments arranged by size: 

                1718    1224     459     209     133
 
TatI w'GTAC_w

Cuts at:    328    3258     328 
   Size:       2930     813
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    669     701    3260    3292     669 
   Size:         32    2559      32    1120

  Fragments arranged by size: 

                2559    1120      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1540    1540 
   Size:       3743
 
XbaI T'CTAG_A

Cuts at:    774     774 
   Size:       3743
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1238    1238 
   Size:       3743
 
XhoI C'TCGA_G

Cuts at:    647     647 
   Size:       3743
 
XmaI C'CCGG_G

Cuts at:    763     763 
   Size:       3743
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII    AlwNI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI    BciVI
     BclI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI
  BsiHKAI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI   BstEII    BstYI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI
     PciI     PmeI     PpiI    PpuMI    PspXI     PstI     PvuI    PvuII
    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AhdI     AleI     AlfI
     AloI     ApaI     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI
    BfrBI     BglI    BmgBI     BmtI   Bpu10I    BsaXI     BsgI    BsiWI
     BsmI    BsmBI    BspEI    BsrGI   BssHII    BstXI  BstZ17I   Bsu36I
     BtgI     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI
  HindIII     HpaI     MfeI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PfoI     PmlI    PshAI     PsiI
   PspOMI     PsrI    SacII    SexAI     SfiI    SgrAI    SnaBI     SrfI
     SspI     StuI     SwaI     XmnI     ZraI
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