Restriction map for
pF1KB3264
(Circular) (Six-base) MAPSORT of: pF1KB3264.seq Check: 949 from: 1 to: 5654
pF1KB3264 5654 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 1784 2692 1784
Size: 908 4746
Acc65I G'GTAC_C
Cuts at: 2670 2670
Size: 5654
AclI AA'CG_TT
Cuts at: 5494 5494
Size: 5654
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 1432 3479 3911 4264 4926 298
Size: 1134 2047 432 353 662 1026
Fragments arranged by size:
2047 1134 1026 662 432 353
AflIII A'CryG_T
Cuts at: 1095 3006 4806 5087 1095
Size: 1911 1800 281 1662
Fragments arranged by size:
1911 1800 1662 281
AleI CACnn'nnGTG
Cuts at: 2632 2632
Size: 5654
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 172 172
Size: 5654
AlwNI CAG_nnn'CTG
Cuts at: 1128 2959 4397 1128
Size: 1831 1438 2385
Fragments arranged by size:
2385 1831 1438
ApaLI G'TGCA_C
Cuts at: 4492 4492
Size: 5654
ApoI r'AATT_y
Cuts at: 796 1057 1275 1325 1549 2028 2311 2658 5007
Size: 261 218 50 224 479 283 347 2349
Cuts at: 5007 5263 796
Size: 256 1187
Fragments arranged by size:
2349 1187 479 347 283 261 256 224
218 50
AseI AT'TA_AT
Cuts at: 20 512 2168 20
Size: 492 1656 3506
Fragments arranged by size:
3506 1656 492
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5654
AvaI C'yCGr_G
Cuts at: 1673 2674 1673
Size: 1001 4653
BamHI G'GATC_C
Cuts at: 1878 2679 1878
Size: 801 4853
BanI G'GyrC_C
Cuts at: 765 2670 3084 3332 3367 765
Size: 1905 414 248 35 3052
Fragments arranged by size:
3052 1905 414 248 35
BanII G_rGCy'C
Cuts at: 2668 3698 2668
Size: 1030 4624
BbeI G_GCGC'C
Cuts at: 3088 3336 3088
Size: 248 5406
BbsI GAAGACnn'nnnn_
Cuts at: 527 527
Size: 5654
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2469 2503 2469
Size: 34 5620
BciVI GTATCCnnnnn_n'
Cuts at: 1968 3552 4608 5654 1968
Size: 1584 1056 1046 1968
Fragments arranged by size:
1968 1584 1056 1046
BclI T'GATC_A
Cuts at: 3174 3174
Size: 5654
BfrBI ATG'CAT
Cuts at: 1662 1662
Size: 5654
BglII A'GATC_T
Cuts at: 579 1443 3169 5206 579
Size: 864 1726 2037 1027
Fragments arranged by size:
2037 1726 1027 864
BlpI GC'TnA_GC
Cuts at: 2766 2766
Size: 5654
Bme1580I G_kGCm'C
Cuts at: 2093 3279 3372 4496 2093
Size: 1186 93 1124 3251
Fragments arranged by size:
3251 1186 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 303 2866 2947 3282 5426 303
Size: 2563 81 335 2144 531
Fragments arranged by size:
2563 2144 531 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 250 4021 250
Size: 3771 1883
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1105 1948 2060 2150 1105
Size: 843 112 90 4609
Fragments arranged by size:
4609 843 112 90
Bpu10I CC'TnA_GC
Cuts at: 191 191
Size: 5654
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2344 2830 3709 4423 4721 2344
Size: 486 879 714 298 3277
Fragments arranged by size:
3277 879 714 486 298
BsaI GGTCTCn'nnnn_
Cuts at: 1228 2149 5358 1228
Size: 921 3209 1524
Fragments arranged by size:
3209 1524 921
BsaAI yAC'GTr
Cuts at: 3007 3007
Size: 5654
BsaBI GATnn'nnATC
Cuts at: 6 3168 5205 6
Size: 3162 2037 455
Fragments arranged by size:
3162 2037 455
BsaHI Gr'CG_yC
Cuts at: 3085 3333 4035 5531 3085
Size: 248 702 1496 3208
Fragments arranged by size:
3208 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2843 3364 4042 4453 4600 4874 2843
Size: 521 678 411 147 274 3623
Fragments arranged by size:
3623 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2534 2564 2534
Size: 30 5624
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1108 2606 1108
Size: 1498 4156
BseYI C'CCAG_C
Cuts at: 1932 2475 3079 3837 4502 4910 1932
Size: 543 604 758 665 408 2676
Fragments arranged by size:
2676 758 665 604 543 408
BsiEI CG_ry'CG
Cuts at: 104 3196 3242 4472 104
Size: 3092 46 1230 1286
Fragments arranged by size:
3092 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1761 2668 3446 3636 4496 1761
Size: 907 778 190 860 2919
Fragments arranged by size:
2919 907 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 352 352
Size: 5654
Bsp1286I G_dGCh'C
Cuts at: 1761 2093 2668 3279 3372 3446 3636 3698 4496
Size: 332 575 611 93 74 190 62 798
Cuts at: 4496 1761
Size: 2919
Fragments arranged by size:
2919 798 611 575 332 190 93 74
62
BspHI T'CATG_A
Cuts at: 864 4086 864
Size: 3222 2432
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1890 2704 2918 3220 3601 1890
Size: 814 214 302 381 3943
Fragments arranged by size:
3943 814 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3946 4000 5484 5649 50
Size: 3896 54 1484 165 55
Fragments arranged by size:
3896 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 144 2141 2294 2480 3566 144
Size: 1997 153 186 1086 2232
Fragments arranged by size:
2232 1997 1086 186 153
BsrFI r'CCGG_y
Cuts at: 3652 3833 3652
Size: 181 5473
BssHII G'CGCG_C
Cuts at: 128 128
Size: 5654
BssSI C'ACGA_G
Cuts at: 3925 4633 3925
Size: 708 4946
BstAPI GCAn_nnn'nTGC
Cuts at: 1694 3031 1694
Size: 1337 4317
BstBI TT'CG_AA
Cuts at: 1329 4015 1329
Size: 2686 2968
BstEII G'GTnAC_C
Cuts at: 174 174
Size: 5654
BstXI CCAn_nnnn'nTGG
Cuts at: 2634 2634
Size: 5654
BstYI r'GATC_y
Cuts at: 1 579 1443 1878 2679 3146 3169 3504 3750
Size: 578 864 435 801 467 23 335 246
Cuts at: 3750 4056 4068 4154 5206 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 864 801 578 467 449 435 335
306 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 292 1310 1708 292
Size: 1018 398 4238
Fragments arranged by size:
4238 1018 398
BtgI C'CryG_G
Cuts at: 107 115 107
Size: 8 5646
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 236 2472 3604 3785 3948 236
Size: 2236 1132 181 163 1942
Fragments arranged by size:
2236 1942 1132 181 163
BtsI GCAGTG_nn'
Cuts at: 130 139 960 1397 2897 130
Size: 9 821 437 1500 2887
Fragments arranged by size:
2887 1500 821 437 9
DraI TTT'AAA
Cuts at: 1103 1574 2316 2653 1103
Size: 471 742 337 4104
Fragments arranged by size:
4104 742 471 337
DraIII CAC_nnn'GTG
Cuts at: 174 174
Size: 5654
DrdI GACnn_nn'nnGTC
Cuts at: 2211 3360 4704 2211
Size: 1149 1344 3161
Fragments arranged by size:
3161 1344 1149
EaeI y'GGCC_r
Cuts at: 473 3239 3413 3804 3831 5176 473
Size: 2766 174 391 27 1345 951
Fragments arranged by size:
2766 1345 951 391 174 27
EagI C'GGCC_G
Cuts at: 3239 3239
Size: 5654
EarI CTCTTCn'nnn_
Cuts at: 1070 1773 2633 3677 3887 1070
Size: 703 860 1044 210 2837
Fragments arranged by size:
2837 1044 860 703 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3862 4604 4750 5464 3862
Size: 742 146 714 4052
Fragments arranged by size:
4052 742 714 146
EcoICRI GAG'CTC
Cuts at: 2666 2666
Size: 5654
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 1105 1432 1948 2060 2150 3479 3911 4264
Size: 807 327 516 112 90 1329 432 353
Cuts at: 4264 4926 298
Size: 662 1026
Fragments arranged by size:
1329 1026 807 662 516 432 353 327
112 90
EcoO109I rG'GnC_Cy
Cuts at: 488 1948 2793 488
Size: 1460 845 3349
Fragments arranged by size:
3349 1460 845
EcoRI G'AATT_C
Cuts at: 2658 2658
Size: 5654
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3673 3673
Size: 5654
FspI TGC'GCA
Cuts at: 209 3435 209
Size: 3226 2428
HaeII r_GCGC'y
Cuts at: 3088 3336 4566 5337 3088
Size: 248 1230 771 3405
Fragments arranged by size:
3405 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3496 3526 3528 3558 3496
Size: 30 2 30 5592
Fragments arranged by size:
5592 30 30 2
HincII GTy'rAC
Cuts at: 2693 2693
Size: 5654
KasI G'GCGC_C
Cuts at: 3084 3332 3084
Size: 248 5406
KpnI G_GTAC'C
Cuts at: 2674 2674
Size: 5654
MfeI C'AATT_G
Cuts at: 1788 1788
Size: 5654
MluI A'CGCG_T
Cuts at: 5087 5087
Size: 5654
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1174 2028 4413 4597 1174
Size: 854 2385 184 2231
Fragments arranged by size:
2385 2231 854 184
MscI TGG'CCA
Cuts at: 475 3415 475
Size: 2940 2714
MslI CAynn'nnrTG
Cuts at: 2566 2632 3643 3770 2566
Size: 66 1011 127 4450
Fragments arranged by size:
4450 1011 127 66
MspA1I CmG'CkG
Cuts at: 243 2425 2765 3079 3439 4223 4468 243
Size: 2182 340 314 360 784 245 1429
Fragments arranged by size:
2182 1429 784 360 340 314 245
NaeI GCC'GGC
Cuts at: 3835 3835
Size: 5654
NarI GG'CG_CC
Cuts at: 3085 3333 3085
Size: 248 5406
NcoI C'CATG_G
Cuts at: 107 107
Size: 5654
NdeI CA'TA_TG
Cuts at: 2631 2631
Size: 5654
NgoMIV G'CCGG_C
Cuts at: 3833 3833
Size: 5654
NsiI A_TGCA'T
Cuts at: 1664 1664
Size: 5654
NspI r_CATG'y
Cuts at: 2707 3207 4810 2707
Size: 500 1603 3551
Fragments arranged by size:
3551 1603 500
PciI A'CATG_T
Cuts at: 4806 4806
Size: 5654
PflMI CCAn_nnn'nTGG
Cuts at: 1128 1128
Size: 5654
PfoI T'CCnGG_A
Cuts at: 1121 1121
Size: 5654
PmeI GTTT'AAAC
Cuts at: 2653 2653
Size: 5654
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4100 4132 4100
Size: 32 5622
PpuMI rG'GwC_Cy
Cuts at: 1948 1948
Size: 5654
PspXI vC'TCGA_Gb
Cuts at: 1673 1673
Size: 5654
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2367 2399 2367
Size: 32 5622
PstI C_TGCA'G
Cuts at: 955 2396 2701 3386 955
Size: 1441 305 685 3223
Fragments arranged by size:
3223 1441 685 305
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5654
PvuII CAG'CTG
Cuts at: 243 2425 3079 3439 243
Size: 2182 654 360 2458
Fragments arranged by size:
2458 2182 654 360
RsrII CG'GwC_CG
Cuts at: 3849 3849
Size: 5654
SacI G_AGCT'C
Cuts at: 2668 2668
Size: 5654
SalI G'TCGA_C
Cuts at: 2691 2691
Size: 5654
SapI GCTCTTCn'nnn_
Cuts at: 3677 3887 3677
Size: 210 5444
SbfI CC_TGCA'GG
Cuts at: 2701 2701
Size: 5654
ScaI AGT'ACT
Cuts at: 949 2184 2341 5171 949
Size: 1235 157 2830 1432
Fragments arranged by size:
2830 1432 1235 157
SfcI C'TryA_G
Cuts at: 33 464 951 1268 2392 2697 3382 4350 4541
Size: 431 487 317 1124 305 685 968 191
Cuts at: 4541 33
Size: 1146
Fragments arranged by size:
1146 1124 968 685 487 431 317 305
191
SfoI GGC'GCC
Cuts at: 3086 3334 3086
Size: 248 5406
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5654
SmaI CCC'GGG
Cuts at: 2676 2676
Size: 5654
SmlI C'TyrA_G
Cuts at: 1673 2359 2809 3724 4438 4700 1673
Size: 686 450 915 714 262 2627
Fragments arranged by size:
2627 915 714 686 450 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5654
SphI G_CATG'C
Cuts at: 2707 3207 2707
Size: 500 5154
SspI AAT'ATT
Cuts at: 757 757
Size: 5654
StuI AGG'CCT
Cuts at: 536 536
Size: 5654
StyI C'CwwG_G
Cuts at: 107 1530 2788 3142 107
Size: 1423 1258 354 2619
Fragments arranged by size:
2619 1423 1258 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2173 3237 3370 3829 4038 2173
Size: 1064 133 459 209 3789
Fragments arranged by size:
3789 1064 459 209 133
TatI w'GTAC_w
Cuts at: 947 1454 2182 2240 2339 5169 947
Size: 507 728 58 99 2830 1432
Fragments arranged by size:
2830 1432 728 507 99 58
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2534 2566 5171 5203 2534
Size: 32 2605 32 2985
Fragments arranged by size:
2985 2605 32 32
Tth111I GACn'n_nGTC
Cuts at: 3451 3451
Size: 5654
XbaI T'CTAG_A
Cuts at: 1341 2685 1341
Size: 1344 4310
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1194 1537 3149 1194
Size: 343 1612 3699
Fragments arranged by size:
3699 1612 343
XhoI C'TCGA_G
Cuts at: 1673 1673
Size: 5654
XmaI C'CCGG_G
Cuts at: 2674 2674
Size: 5654
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PciI PflMI PfoI PmeI PpiI PpuMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AloI ApaI
AscI AvrII BaeI BbvCI BglI BmgBI BmtI BsgI
BsiWI BsmI BspEI BsrGI BstZ17I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI NheI NotI NruI
PacI PasI PmlI PshAI PsiI PspOMI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SwaI XmnI ZraI