Restriction map for
pF1KB3279
(Circular) (Six-base) MAPSORT of: pF1KB3279.seq Check: 8105 from: 1 to: 5963
pF1KB3279 5963 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1248 1752 1248
Size: 504 5459
AccI GT'mk_AC
Cuts at: 437 847 3001 437
Size: 410 2154 3399
Fragments arranged by size:
3399 2154 410
Acc65I G'GTAC_C
Cuts at: 2979 2979
Size: 5963
AclI AA'CG_TT
Cuts at: 869 5803 869
Size: 4934 1029
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2406 3788 4220 4573 5235 2406
Size: 1382 432 353 662 3134
Fragments arranged by size:
3134 1382 662 432 353
AfeI AGC'GCT
Cuts at: 1612 2081 1612
Size: 469 5494
AflII C'TTAA_G
Cuts at: 999 999
Size: 5963
AflIII A'CryG_T
Cuts at: 1628 1919 2008 2198 3315 5115 5396 1628
Size: 291 89 190 1117 1800 281 2195
Fragments arranged by size:
2195 1800 1117 291 281 190 89
AleI CACnn'nnGTG
Cuts at: 238 238
Size: 5963
AlwNI CAG_nnn'CTG
Cuts at: 261 3268 4706 261
Size: 3007 1438 1518
Fragments arranged by size:
3007 1518 1438
ApaI G_GGCC'C
Cuts at: 2173 2173
Size: 5963
ApaLI G'TGCA_C
Cuts at: 1636 2566 4801 1636
Size: 930 2235 2798
Fragments arranged by size:
2798 2235 930
ApoI r'AATT_y
Cuts at: 366 2767 2967 5316 5572 366
Size: 2401 200 2349 256 757
Fragments arranged by size:
2401 2349 757 256 200
AseI AT'TA_AT
Cuts at: 20 20
Size: 5963
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5963
AvaI C'yCGr_G
Cuts at: 766 1908 2173 2427 2983 766
Size: 1142 265 254 556 3746
Fragments arranged by size:
3746 1142 556 265 254
BamHI G'GATC_C
Cuts at: 2988 2988
Size: 5963
BanI G'GyrC_C
Cuts at: 232 285 778 1864 2130 2161 2979 3393 3641
Size: 53 493 1086 266 31 818 414 248
Cuts at: 3641 3676 232
Size: 35 2519
Fragments arranged by size:
2519 1086 818 493 414 266 248 53
35 31
BanII G_rGCy'C
Cuts at: 628 1344 1402 2173 2977 4007 628
Size: 716 58 771 804 1030 2584
Fragments arranged by size:
2584 1030 804 771 716 58
BbeI G_GCGC'C
Cuts at: 1868 2134 3397 3645 1868
Size: 266 1263 248 4186
Fragments arranged by size:
4186 1263 266 248
BbsI GAAGACnn'nnnn_
Cuts at: 2410 2410
Size: 5963
BciVI GTATCCnnnnn_n'
Cuts at: 2643 3861 4917 5963 2643
Size: 1218 1056 1046 2643
Fragments arranged by size:
2643 1218 1056 1046
BclI T'GATC_A
Cuts at: 2207 2613 3483 2207
Size: 406 870 4687
Fragments arranged by size:
4687 870 406
BglII A'GATC_T
Cuts at: 3478 5515 3478
Size: 2037 3926
BlpI GC'TnA_GC
Cuts at: 3075 3075
Size: 5963
Bme1580I G_kGCm'C
Cuts at: 1640 1825 2016 2173 2570 3588 3681 4805 1640
Size: 185 191 157 397 1018 93 1124 2798
Fragments arranged by size:
2798 1124 1018 397 191 185 157 93
BmgBI CAC'GTC
Cuts at: 468 651 1284 1449 468
Size: 183 633 165 4982
Fragments arranged by size:
4982 633 183 165
BmrI ACTGGGnnnn_n'
Cuts at: 820 1057 1210 2504 3175 3256 3591 5735 820
Size: 237 153 1294 671 81 335 2144 1048
Fragments arranged by size:
2144 1294 1048 671 335 237 153 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4330 4330
Size: 5963
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 352 445 482 936 950 1711 2660 289
Size: 63 93 37 454 14 761 949 3592
Fragments arranged by size:
3592 949 761 454 93 63 37 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 102 3139 4018 4732 5030 102
Size: 3037 879 714 298 1035
Fragments arranged by size:
3037 1035 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2863 5667 2863
Size: 2804 3159
BsaAI yAC'GTr
Cuts at: 1629 3316 1629
Size: 1687 4276
BsaBI GATnn'nnATC
Cuts at: 6 3477 5514 6
Size: 3471 2037 455
Fragments arranged by size:
3471 2037 455
BsaHI Gr'CG_yC
Cuts at: 1276 1865 1901 2131 2152 3394 3642 4344 5840
Size: 589 36 230 21 1242 248 702 1496
Cuts at: 5840 1276
Size: 1399
Fragments arranged by size:
1496 1399 1242 702 589 248 230 36
21
BsaWI w'CCGG_w
Cuts at: 723 2743 3152 3673 4351 4762 4909 5183 723
Size: 2020 409 521 678 411 147 274 1503
Fragments arranged by size:
2020 1503 678 521 411 409 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 934 964 1585 1615 2521 2551 934
Size: 30 621 30 906 30 4346
Fragments arranged by size:
4346 906 621 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 140 400 1699 2382 2590 2881 140
Size: 260 1299 683 208 291 3222
Fragments arranged by size:
3222 1299 683 291 260 208
BseYI C'CCAG_C
Cuts at: 507 3388 4146 4811 5219 507
Size: 2881 758 665 408 1251
Fragments arranged by size:
2881 1251 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 432 432
Size: 5963
BsiEI CG_ry'CG
Cuts at: 104 381 1858 3505 3551 4781 104
Size: 277 1477 1647 46 1230 1286
Fragments arranged by size:
1647 1477 1286 1230 277 46
BsiHKAI G_wGCw'C
Cuts at: 1053 1344 1640 2278 2386 2570 2977 3755 3945
Size: 291 296 638 108 184 407 778 190
Cuts at: 3945 4805 1053
Size: 860 2211
Fragments arranged by size:
2211 860 778 638 407 296 291 190
184 108
BsmI GAATG_Cn'
Cuts at: 2585 2585
Size: 5963
Bsp1286I G_dGCh'C
Cuts at: 628 1053 1344 1402 1640 1825 2016 2173 2278
Size: 425 291 58 238 185 191 157 105
Cuts at: 2278 2386 2570 2977 3588 3681 3755 3945 4007
Size: 108 184 407 611 93 74 190 62
Cuts at: 4007 4805 628
Size: 798 1786
Fragments arranged by size:
1786 798 611 425 407 291 238 191
190 185 184 157 108 105 93 74
62 58
BspEI T'CCGG_A
Cuts at: 723 723
Size: 5963
BspHI T'CATG_A
Cuts at: 4395 4395
Size: 5963
BspMI ACCTGCnnnn'nnnn_
Cuts at: 441 660 1248 1273 1752 3013 3227 3529 3910
Size: 219 588 25 479 1261 214 302 381
Cuts at: 3910 441
Size: 2494
Fragments arranged by size:
2494 1261 588 479 381 302 219 214
25
BsrBI CCG'CTC
Cuts at: 50 191 1596 1649 2123 4255 4309 5793 5958
Size: 141 1405 53 474 2132 54 1484 165
Cuts at: 5958 50
Size: 55
Fragments arranged by size:
2132 1484 1405 474 165 141 55 54
53
BsrDI GCAATG_nn'
Cuts at: 179 3875 179
Size: 3696 2267
BsrFI r'CCGG_y
Cuts at: 1794 1867 3961 4142 1794
Size: 73 2094 181 3615
Fragments arranged by size:
3615 2094 181 73
BsrGI T'GTAC_A
Cuts at: 1108 1985 2258 1108
Size: 877 273 4813
Fragments arranged by size:
4813 877 273
BssHII G'CGCG_C
Cuts at: 1715 1717 1890 2303 1715
Size: 2 173 413 5375
Fragments arranged by size:
5375 413 173 2
BssSI C'ACGA_G
Cuts at: 1681 4234 4942 1681
Size: 2553 708 2702
Fragments arranged by size:
2702 2553 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3340 3340
Size: 5963
BstBI TT'CG_AA
Cuts at: 4324 4324
Size: 5963
BstEII G'GTnAC_C
Cuts at: 1260 1380 1260
Size: 120 5843
BstXI CCAn_nnnn'nTGG
Cuts at: 2103 2103
Size: 5963
BstYI r'GATC_y
Cuts at: 1 129 1058 2449 2782 2988 3455 3478 3813
Size: 128 929 1391 333 206 467 23 335
Cuts at: 3813 4059 4365 4377 4463 5515 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1391 1052 929 467 449 335 333 306
246 206 128 86 23 12
BstZ17I GTA'TAC
Cuts at: 848 848
Size: 5963
BtgI C'CryG_G
Cuts at: 318 1418 2067 2090 2165 318
Size: 1100 649 23 75 4116
Fragments arranged by size:
4116 1100 649 75 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 234 516 2196 2296 3913 4094 4257 234
Size: 282 1680 100 1617 181 163 1940
Fragments arranged by size:
1940 1680 1617 282 181 163 100
BtsI GCAGTG_nn'
Cuts at: 1841 1901 2626 3206 1841
Size: 60 725 580 4598
Fragments arranged by size:
4598 725 580 60
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 161 196 161
Size: 35 5928
DraI TTT'AAA
Cuts at: 2715 2962 2715
Size: 247 5716
DrdI GACnn_nn'nnGTC
Cuts at: 3669 5013 3669
Size: 1344 4619
EaeI y'GGCC_r
Cuts at: 192 746 1421 1442 1952 2087 2093 2124 2623
Size: 554 675 21 510 135 6 31 499
Cuts at: 2623 3548 3722 4113 4140 5485 192
Size: 925 174 391 27 1345 670
Fragments arranged by size:
1345 925 675 670 554 510 499 391
174 135 31 27 21 6
EagI C'GGCC_G
Cuts at: 3548 3548
Size: 5963
EarI CTCTTCn'nnn_
Cuts at: 3986 4196 3986
Size: 210 5753
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 292 414 1353 4171 4913 5059 5773 292
Size: 122 939 2818 742 146 714 482
Fragments arranged by size:
2818 939 742 714 482 146 122
EcoICRI GAG'CTC
Cuts at: 1342 2975 1342
Size: 1633 4330
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 352 445 482 936 950 1711 2406 2660
Size: 63 93 37 454 14 761 695 254
Cuts at: 2660 3788 4220 4573 5235 289
Size: 1128 432 353 662 1017
Fragments arranged by size:
1128 1017 761 695 662 454 432 353
254 93 63 37 14
EcoNI CCTnn'n_nnAGG
Cuts at: 331 2575 331
Size: 2244 3719
EcoO109I rG'GnC_Cy
Cuts at: 258 324 2170 2608 3102 258
Size: 66 1846 438 494 3119
Fragments arranged by size:
3119 1846 494 438 66
EcoRI G'AATT_C
Cuts at: 366 2967 366
Size: 2601 3362
EcoRV GAT'ATC
Cuts at: 591 591
Size: 5963
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3982 3982
Size: 5963
FspI TGC'GCA
Cuts at: 3744 3744
Size: 5963
HaeII r_GCGC'y
Cuts at: 1614 1801 1868 2046 2083 2134 3397 3645 4875
Size: 187 67 178 37 51 1263 248 1230
Cuts at: 4875 5646 1614
Size: 771 1931
Fragments arranged by size:
1931 1263 1230 771 248 187 178 67
51 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 562 594 961 993 1345 1377 1384 1416 1891
Size: 32 367 32 352 32 7 32 475
Cuts at: 1891 1923 3805 3835 3837 3867 562
Size: 32 1882 30 2 30 2658
Fragments arranged by size:
2658 1882 475 367 352 32 32 32
32 32 30 30 7 2
HincII GTy'rAC
Cuts at: 1157 2196 2694 3002 1157
Size: 1039 498 308 4118
Fragments arranged by size:
4118 1039 498 308
KasI G'GCGC_C
Cuts at: 1864 2130 3393 3641 1864
Size: 266 1263 248 4186
Fragments arranged by size:
4186 1263 266 248
KpnI G_GTAC'C
Cuts at: 2983 2983
Size: 5963
MluI A'CGCG_T
Cuts at: 1919 2008 5396 1919
Size: 89 3388 2486
Fragments arranged by size:
3388 2486 89
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 284 1655 4722 4906 284
Size: 1371 3067 184 1341
Fragments arranged by size:
3067 1371 1341 184
MscI TGG'CCA
Cuts at: 2625 3724 2625
Size: 1099 4864
MslI CAynn'nnrTG
Cuts at: 238 899 3952 4079 238
Size: 661 3053 127 2122
Fragments arranged by size:
3053 2122 661 127
MspA1I CmG'CkG
Cuts at: 320 447 2705 3074 3388 3748 4532 4777 320
Size: 127 2258 369 314 360 784 245 1506
Fragments arranged by size:
2258 1506 784 369 360 314 245 127
NaeI GCC'GGC
Cuts at: 1796 1869 4144 1796
Size: 73 2275 3615
Fragments arranged by size:
3615 2275 73
NarI GG'CG_CC
Cuts at: 1865 2131 3394 3642 1865
Size: 266 1263 248 4186
Fragments arranged by size:
4186 1263 266 248
NdeI CA'TA_TG
Cuts at: 1028 1028
Size: 5963
NgoMIV G'CCGG_C
Cuts at: 1794 1867 4142 1794
Size: 73 2275 3615
Fragments arranged by size:
3615 2275 73
NspI r_CATG'y
Cuts at: 2556 3016 3516 5119 2556
Size: 460 500 1603 3400
Fragments arranged by size:
3400 1603 500 460
PciI A'CATG_T
Cuts at: 5115 5115
Size: 5963
PfoI T'CCnGG_A
Cuts at: 212 644 2601 212
Size: 432 1957 3574
Fragments arranged by size:
3574 1957 432
PmeI GTTT'AAAC
Cuts at: 2962 2962
Size: 5963
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4409 4441 4409
Size: 32 5931
PpuMI rG'GwC_Cy
Cuts at: 258 324 258
Size: 66 5897
PspOMI G'GGCC_C
Cuts at: 2169 2169
Size: 5963
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 831 863 1477 1509 831
Size: 32 614 32 5285
Fragments arranged by size:
5285 614 32 32
PstI C_TGCA'G
Cuts at: 347 452 1670 1763 1943 3010 3695 347
Size: 105 1218 93 180 1067 685 2615
Fragments arranged by size:
2615 1218 1067 685 180 105 93
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5963
PvuII CAG'CTG
Cuts at: 2705 3388 3748 2705
Size: 683 360 4920
Fragments arranged by size:
4920 683 360
RsrII CG'GwC_CG
Cuts at: 4158 4158
Size: 5963
SacI G_AGCT'C
Cuts at: 1344 2977 1344
Size: 1633 4330
SacII CC_GC'GG
Cuts at: 321 321
Size: 5963
SalI G'TCGA_C
Cuts at: 3000 3000
Size: 5963
SapI GCTCTTCn'nnn_
Cuts at: 3986 4196 3986
Size: 210 5753
SbfI CC_TGCA'GG
Cuts at: 3010 3010
Size: 5963
ScaI AGT'ACT
Cuts at: 5480 5480
Size: 5963
SexAI A'CCwGG_T
Cuts at: 2954 2954
Size: 5963
SfcI C'TryA_G
Cuts at: 33 343 448 1666 1699 1759 1939 2453 3006
Size: 310 105 1218 33 60 180 514 553
Cuts at: 3006 3691 4659 4850 33
Size: 685 968 191 1146
Fragments arranged by size:
1218 1146 968 685 553 514 310 191
180 105 60 33
SfoI GGC'GCC
Cuts at: 1866 2132 3395 3643 1866
Size: 266 1263 248 4186
Fragments arranged by size:
4186 1263 266 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5963
SgrAI Cr'CCGG_yG
Cuts at: 1794 1867 1794
Size: 73 5890
SmaI CCC'GGG
Cuts at: 2429 2985 2429
Size: 556 5407
SmlI C'TyrA_G
Cuts at: 117 999 3118 4033 4747 5009 117
Size: 882 2119 915 714 262 1071
Fragments arranged by size:
2119 1071 915 882 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5963
SphI G_CATG'C
Cuts at: 2556 3016 3516 2556
Size: 460 500 5003
Fragments arranged by size:
5003 500 460
SrfI GCCC'GGGC
Cuts at: 2429 2429
Size: 5963
StyI C'CwwG_G
Cuts at: 3097 3451 3097
Size: 354 5609
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 168 2149 2422 3546 3679 4138 4347 168
Size: 1981 273 1124 133 459 209 1784
Fragments arranged by size:
1981 1784 1124 459 273 209 133
TatI w'GTAC_w
Cuts at: 1108 1484 1985 2258 5478 1108
Size: 376 501 273 3220 1593
Fragments arranged by size:
3220 1593 501 376 273
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 872 904 1043 1075 5480 5512 872
Size: 32 139 32 4405 32 1323
Fragments arranged by size:
4405 1323 139 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3760 3760
Size: 5963
XbaI T'CTAG_A
Cuts at: 2994 2994
Size: 5963
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 987 1431 3458 987
Size: 444 2027 3492
Fragments arranged by size:
3492 2027 444
XmaI C'CCGG_G
Cuts at: 2427 2983 2427
Size: 556 5407
XmnI GAAnn'nnTTC
Cuts at: 526 526
Size: 5963
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NspI PciI PfoI PmeI PpiI
PpuMI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI SrfI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AgeI AhdI AlfI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmtI Bpu10I BsiWI BsmBI
Bsu36I ClaI DraIII FseI FspAI HindIII HpaI MfeI
NcoI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PshAI PsiI PspXI SanDI SfiI SnaBI SspI
StuI SwaI XhoI ZraI