Restriction map for
pF1KB3283
(Circular) (Six-base) MAPSORT of: pF1KB3283.seq Check: 9376 from: 1 to: 6680
pF1KB3283 6680 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 598 824 1787 3608 598
Size: 226 963 1821 3670
Fragments arranged by size:
3670 1821 963 226
AccI GT'mk_AC
Cuts at: 668 3718 668
Size: 3050 3630
Acc65I G'GTAC_C
Cuts at: 1016 3696 1016
Size: 2680 4000
AclI AA'CG_TT
Cuts at: 6520 6520
Size: 6680
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 687 1264 1537 4505 4937 5684 5952 151
Size: 536 577 273 2968 432 747 268 879
Fragments arranged by size:
2968 879 747 577 536 432 273 268
AflIII A'CryG_T
Cuts at: 3272 4032 5136 6113 3272
Size: 760 1104 977 3839
Fragments arranged by size:
3839 1104 977 760
AgeI A'CCGG_T
Cuts at: 5068 5068
Size: 6680
AleI CACnn'nnGTG
Cuts at: 1044 1044
Size: 6680
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2152 3175 2152
Size: 1023 5657
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2516 2548 2516
Size: 32 6648
AlwNI CAG_nnn'CTG
Cuts at: 925 1217 1420 1999 2808 3495 3985 5552 925
Size: 292 203 579 809 687 490 1567 2053
Fragments arranged by size:
2053 1567 809 687 579 490 292 203
ApaI G_GGCC'C
Cuts at: 313 349 1338 1848 2208 2363 313
Size: 36 989 510 360 155 4630
Fragments arranged by size:
4630 989 510 360 155 36
ApaLI G'TGCA_C
Cuts at: 2049 5450 2049
Size: 3401 3279
ApoI r'AATT_y
Cuts at: 3684 6033 6289 3684
Size: 2349 256 4075
Fragments arranged by size:
4075 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6680
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6680
AvaI C'yCGr_G
Cuts at: 203 1357 1488 1545 2195 2209 2571 3700 203
Size: 1154 131 57 650 14 362 1129 3183
Fragments arranged by size:
3183 1154 1129 650 362 131 57 14
BamHI G'GATC_C
Cuts at: 735 2735 3705 735
Size: 2000 970 3710
Fragments arranged by size:
3710 2000 970
BanI G'GyrC_C
Cuts at: 136 582 812 1016 1046 1159 3188 3340 3355
Size: 446 230 204 30 113 2029 152 15
Cuts at: 3355 3395 3462 3696 4110 4358 4393 136
Size: 40 67 234 414 248 35 2423
Fragments arranged by size:
2423 2029 446 414 248 234 230 204
152 113 67 40 35 30 15
BanII G_rGCy'C
Cuts at: 191 313 349 573 767 1338 1848 2208 2363
Size: 122 36 224 194 571 510 360 155
Cuts at: 2363 2660 2815 2908 3065 3094 3236 3477 3694
Size: 297 155 93 157 29 142 241 217
Cuts at: 3694 4724 191
Size: 1030 2147
Fragments arranged by size:
2147 1030 571 510 360 297 241 224
217 194 157 155 155 142 122 93
36 29
BbeI G_GCGC'C
Cuts at: 3344 3359 3399 4114 4362 3344
Size: 15 40 715 248 5662
Fragments arranged by size:
5662 715 248 40 15
BbsI GAAGACnn'nnnn_
Cuts at: 160 1255 1703 160
Size: 1095 448 5137
Fragments arranged by size:
5137 1095 448
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1749 1783 1749
Size: 34 6646
BciVI GTATCCnnnnn_n'
Cuts at: 4578 5339 6680 4578
Size: 761 1341 4578
Fragments arranged by size:
4578 1341 761
BclI T'GATC_A
Cuts at: 4200 4200
Size: 6680
BglI GCCn_nnn'nGGC
Cuts at: 832 1145 3186 832
Size: 313 2041 4326
Fragments arranged by size:
4326 2041 313
BglII A'GATC_T
Cuts at: 4195 6232 4195
Size: 2037 4643
BlpI GC'TnA_GC
Cuts at: 1756 2125 2809 3557 3792 1756
Size: 369 684 748 235 4644
Fragments arranged by size:
4644 748 684 369 235
Bme1580I G_kGCm'C
Cuts at: 139 313 349 454 587 695 815 932 1051
Size: 174 36 105 133 108 120 117 119
Cuts at: 1051 1338 1611 1637 1848 2053 2069 2174 2208
Size: 287 273 26 211 205 16 105 34
Cuts at: 2208 2363 2787 3198 3285 3328 4305 4398 5454
Size: 155 424 411 87 43 977 93 1056
Cuts at: 5454 139
Size: 1365
Fragments arranged by size:
1365 1056 977 424 411 287 273 211
205 174 155 133 120 119 117 108
105 105 93 87 43 36 34 26
16
BmrI ACTGGGnnnn_n'
Cuts at: 2009 2088 2465 3630 3892 3973 4308 6452 2009
Size: 79 377 1165 262 81 335 2144 2237
Fragments arranged by size:
2237 2144 1165 377 335 262 81 79
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1685 2886 5047 1685
Size: 1201 2161 3318
Fragments arranged by size:
3318 2161 1201
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 583 1171 1522 1640 1698 2088 2591 2724
Size: 15 588 351 118 58 390 503 133
Cuts at: 2724 3079 3298 3367 3421 568
Size: 355 219 69 54 3827
Fragments arranged by size:
3827 588 503 390 355 351 219 133
118 69 58 54 15
Bpu10I CC'TnA_GC
Cuts at: 268 479 708 1058 2879 268
Size: 211 229 350 1821 4069
Fragments arranged by size:
4069 1821 350 229 211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 3856 4735 5227 5525 484
Size: 3372 879 492 298 1639
Fragments arranged by size:
3372 1639 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 544 2305 2323 3245 3532 6384 544
Size: 1761 18 922 287 2852 840
Fragments arranged by size:
2852 1761 922 840 287 18
BsaAI yAC'GTr
Cuts at: 3273 4033 3273
Size: 760 5920
BsaBI GATnn'nnATC
Cuts at: 6 4194 6231 6
Size: 4188 2037 455
Fragments arranged by size:
4188 2037 455
BsaHI Gr'CG_yC
Cuts at: 263 3290 3341 3356 3396 4111 4359 5061 6557
Size: 3027 51 15 40 715 248 702 1496
Cuts at: 6557 263
Size: 386
Fragments arranged by size:
3027 1496 715 702 386 248 51 40
15
BsaWI w'CCGG_w
Cuts at: 1401 2985 3869 4390 5068 5342 5489 5900 1401
Size: 1584 884 521 678 274 147 411 2181
Fragments arranged by size:
2181 1584 884 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2783 2813 3440 3470 3514 3544 2783
Size: 30 627 30 44 30 5919
Fragments arranged by size:
5919 627 44 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1324 1643 2059 2323 3421 1324
Size: 319 416 264 1098 4583
Fragments arranged by size:
4583 1098 416 319 264
BseYI C'CCAG_C
Cuts at: 121 378 991 1023 1073 1180 1225 1435 1847
Size: 257 613 32 50 107 45 210 412
Cuts at: 1847 2334 2660 2828 2833 3065 3293 3577 4105
Size: 487 326 168 5 232 228 284 528
Cuts at: 4105 4863 5440 5936 121
Size: 758 577 496 865
Fragments arranged by size:
865 758 613 577 528 496 487 412
326 284 257 232 228 210 168 107
50 45 32 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 835 2821 2905 3508 199
Size: 636 1986 84 603 3371
Fragments arranged by size:
3371 1986 636 603 84
BsiEI CG_ry'CG
Cuts at: 104 3332 4222 4268 5476 104
Size: 3228 890 46 1208 1308
Fragments arranged by size:
3228 1308 1208 890 46
BsiHKAI G_wGCw'C
Cuts at: 274 573 2053 2131 2702 3505 3694 4472 4662
Size: 299 1480 78 571 803 189 778 190
Cuts at: 4662 5454 274
Size: 792 1500
Fragments arranged by size:
1500 1480 803 792 778 571 299 190
189 78
BsmI GAATG_Cn'
Cuts at: 433 743 1892 433
Size: 310 1149 5221
Fragments arranged by size:
5221 1149 310
Bsp1286I G_dGCh'C
Cuts at: 139 191 274 313 349 454 573 587 695
Size: 52 83 39 36 105 119 14 108
Cuts at: 695 767 815 932 1051 1338 1611 1637 1848
Size: 72 48 117 119 287 273 26 211
Cuts at: 1848 2053 2069 2131 2174 2208 2363 2660 2702
Size: 205 16 62 43 34 155 297 42
Cuts at: 2702 2787 2815 2908 3065 3094 3198 3236 3285
Size: 85 28 93 157 29 104 38 49
Cuts at: 3285 3328 3477 3505 3694 4305 4398 4472 4662
Size: 43 149 28 189 611 93 74 190
Cuts at: 4662 4724 5454 139
Size: 62 730 1365
Fragments arranged by size:
1365 730 611 297 287 273 211 205
190 189 157 155 149 119 119 117
108 105 104 93 93 85 83 74
72 62 62 52 49 48 43 43
42 39 38 36 34 29 28 28
26 16 14
BspEI T'CCGG_A
Cuts at: 2985 5900 2985
Size: 2915 3765
BspHI T'CATG_A
Cuts at: 5856 5856
Size: 6680
BspMI ACCTGCnnnn'nnnn_
Cuts at: 598 824 1787 3608 3730 3944 4246 4627 598
Size: 226 963 1821 122 214 302 381 2651
Fragments arranged by size:
2651 1821 963 381 302 226 214 122
BsrBI CCG'CTC
Cuts at: 50 903 1827 4972 5026 6510 6675 50
Size: 853 924 3145 54 1484 165 55
Fragments arranged by size:
3145 1484 924 853 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1379 2141 2280 2746 4592 1379
Size: 762 139 466 1846 3467
Fragments arranged by size:
3467 1846 762 466 139
BsrFI r'CCGG_y
Cuts at: 881 1317 1751 1964 4678 4859 5068 881
Size: 436 434 213 2714 181 209 2493
Fragments arranged by size:
2714 2493 436 434 213 209 181
BssSI C'ACGA_G
Cuts at: 4951 5309 4951
Size: 358 6322
BstAPI GCAn_nnn'nTGC
Cuts at: 1452 1913 4057 1452
Size: 461 2144 4075
Fragments arranged by size:
4075 2144 461
BstBI TT'CG_AA
Cuts at: 5041 5041
Size: 6680
BstXI CCAn_nnnn'nTGG
Cuts at: 284 561 641 862 1618 2496 2841 2955 284
Size: 277 80 221 756 878 345 114 4009
Fragments arranged by size:
4009 878 756 345 277 221 114 80
BstYI r'GATC_y
Cuts at: 1 557 735 1116 2531 2735 3705 4172 4195
Size: 556 178 381 1415 204 970 467 23
Cuts at: 4195 4530 4776 5788 5874 5886 6232 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1415 1012 970 556 467 449 381 346
335 246 204 178 86 23 12
BstZ17I GTA'TAC
Cuts at: 669 669
Size: 6680
Bsu36I CC'TnA_GG
Cuts at: 1301 1301
Size: 6680
BtgI C'CryG_G
Cuts at: 107 219 1920 2039 2402 3003 3650 107
Size: 112 1701 119 363 601 647 3137
Fragments arranged by size:
3137 1701 647 601 363 119 112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1016 1959 4630 4811 4974 1016
Size: 943 2671 181 163 2722
Fragments arranged by size:
2722 2671 943 181 163
BtsI GCAGTG_nn'
Cuts at: 531 847 1418 2129 2172 3175 3644 3923 531
Size: 316 571 711 43 1003 469 279 3288
Fragments arranged by size:
3288 1003 711 571 469 316 279 43
ClaI AT'CG_AT
Cuts at: 1465 1465
Size: 6680
DraI TTT'AAA
Cuts at: 3679 3679
Size: 6680
DraIII CAC_nnn'GTG
Cuts at: 3266 3486 3266
Size: 220 6460
DrdI GACnn_nn'nnGTC
Cuts at: 4386 5244 4386
Size: 858 5822
EaeI y'GGCC_r
Cuts at: 492 879 1396 1524 2352 3137 3329 3647 4265
Size: 387 517 128 828 785 192 318 618
Cuts at: 4265 4439 4830 4857 6202 492
Size: 174 391 27 1345 970
Fragments arranged by size:
1345 970 828 785 618 517 391 387
318 192 174 128 27
EagI C'GGCC_G
Cuts at: 3329 4265 3329
Size: 936 5744
EarI CTCTTCn'nnn_
Cuts at: 910 1933 2695 4703 4913 910
Size: 1023 762 2008 210 2677
Fragments arranged by size:
2677 2008 1023 762 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3292 4888 5198 5344 6490 3292
Size: 1596 310 146 1146 3482
Fragments arranged by size:
3482 1596 1146 310 146
EcoICRI GAG'CTC
Cuts at: 571 3692 571
Size: 3121 3559
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 568 583 687 1171 1264 1522 1537 1640
Size: 417 15 104 484 93 258 15 103
Cuts at: 1640 1698 2088 2591 2724 3079 3298 3367 3421
Size: 58 390 503 133 355 219 69 54
Cuts at: 3421 4505 4937 5684 5952 151
Size: 1084 432 747 268 879
Fragments arranged by size:
1084 879 747 503 484 432 417 390
355 268 258 219 133 104 103 93
69 58 54 15 15
EcoNI CCTnn'n_nnAGG
Cuts at: 255 1412 1673 2630 2692 3403 3436 255
Size: 1157 261 957 62 711 33 3499
Fragments arranged by size:
3499 1157 957 711 261 62 33
EcoO109I rG'GnC_Cy
Cuts at: 309 310 345 346 1146 1214 1335 1513 1542
Size: 1 35 1 800 68 121 178 29
Cuts at: 1542 1833 2205 2359 2360 2586 3441 3492 3574
Size: 291 372 154 1 226 855 51 82
Cuts at: 3574 3819 309
Size: 245 3170
Fragments arranged by size:
3170 855 800 372 291 245 226 178
154 121 82 68 51 35 29 1
1 1
EcoRI G'AATT_C
Cuts at: 3684 3684
Size: 6680
EcoRV GAT'ATC
Cuts at: 2163 2163
Size: 6680
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 783 3226 4699 783
Size: 2443 1473 2764
Fragments arranged by size:
2764 2443 1473
FspI TGC'GCA
Cuts at: 2581 4461 2581
Size: 1880 4800
HaeII r_GCGC'y
Cuts at: 2809 3344 3359 3399 4114 4362 5384 6363 2809
Size: 535 15 40 715 248 1022 979 3126
Fragments arranged by size:
3126 1022 979 715 535 248 40 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1471 1503 2760 2792 2890 2922 4522 4552 4554
Size: 32 1257 32 98 32 1600 30 2
Cuts at: 4554 4584 1471
Size: 30 3567
Fragments arranged by size:
3567 1600 1257 98 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 372 3719 372
Size: 3347 3333
KasI G'GCGC_C
Cuts at: 3340 3355 3395 4110 4358 3340
Size: 15 40 715 248 5662
Fragments arranged by size:
5662 715 248 40 15
KpnI G_GTAC'C
Cuts at: 1020 3700 1020
Size: 2680 4000
MluI A'CGCG_T
Cuts at: 6113 6113
Size: 6680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5351 5535 5351
Size: 184 6496
MscI TGG'CCA
Cuts at: 494 1526 4441 494
Size: 1032 2915 2733
Fragments arranged by size:
2915 2733 1032
MslI CAynn'nnrTG
Cuts at: 488 635 1044 2134 3157 4669 4796 488
Size: 147 409 1090 1023 1512 127 2372
Fragments arranged by size:
2372 1512 1090 1023 409 147 127
MspA1I CmG'CkG
Cuts at: 228 429 775 1023 1077 1618 1851 1955 2041
Size: 201 346 248 54 541 233 104 86
Cuts at: 2041 2326 2404 3368 3791 4105 4465 5478 5723
Size: 285 78 964 423 314 360 1013 245
Cuts at: 5723 228
Size: 1185
Fragments arranged by size:
1185 1013 964 541 423 360 346 314
285 248 245 233 201 104 86 78
54
NaeI GCC'GGC
Cuts at: 883 1753 4861 883
Size: 870 3108 2702
Fragments arranged by size:
3108 2702 870
NarI GG'CG_CC
Cuts at: 3341 3356 3396 4111 4359 3341
Size: 15 40 715 248 5662
Fragments arranged by size:
5662 715 248 40 15
NcoI C'CATG_G
Cuts at: 107 219 1920 3003 107
Size: 112 1701 1083 3784
Fragments arranged by size:
3784 1701 1083 112
NgoMIV G'CCGG_C
Cuts at: 881 1751 4859 881
Size: 870 3108 2702
Fragments arranged by size:
3108 2702 870
NspI r_CATG'y
Cuts at: 324 3166 3733 4233 5140 324
Size: 2842 567 500 907 1864
Fragments arranged by size:
2842 1864 907 567 500
PasI CC'CwG_GG
Cuts at: 306 662 1211 1732 2547 2632 306
Size: 356 549 521 815 85 4354
Fragments arranged by size:
4354 815 549 521 356 85
PciI A'CATG_T
Cuts at: 5136 5136
Size: 6680
PflMI CCAn_nnn'nTGG
Cuts at: 429 1003 2115 429
Size: 574 1112 4994
Fragments arranged by size:
4994 1112 574
PfoI T'CCnGG_A
Cuts at: 560 1538 2495 2534 2693 560
Size: 978 957 39 159 4547
Fragments arranged by size:
4547 978 957 159 39
PmeI GTTT'AAAC
Cuts at: 3679 3679
Size: 6680
PmlI CAC'GTG
Cuts at: 3273 3273
Size: 6680
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1186 1218 5819 5851 1186
Size: 32 4601 32 2015
Fragments arranged by size:
4601 2015 32 32
PpuMI rG'GwC_Cy
Cuts at: 1513 1542 3574 1513
Size: 29 2032 4619
Fragments arranged by size:
4619 2032 29
PspOMI G'GGCC_C
Cuts at: 309 345 1334 1844 2204 2359 309
Size: 36 989 510 360 155 4630
Fragments arranged by size:
4630 989 510 360 155 36
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1000 1032 1000
Size: 32 6648
PstI C_TGCA'G
Cuts at: 821 1425 1688 1909 1960 2654 3471 3727 4412
Size: 604 263 221 51 694 817 256 685
Cuts at: 4412 821
Size: 3089
Fragments arranged by size:
3089 817 694 685 604 263 256 221
51
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6680
PvuII CAG'CTG
Cuts at: 775 1023 1618 3368 4105 4465 775
Size: 248 595 1750 737 360 2990
Fragments arranged by size:
2990 1750 737 595 360 248
RsrII CG'GwC_CG
Cuts at: 4875 4875
Size: 6680
SacI G_AGCT'C
Cuts at: 573 3694 573
Size: 3121 3559
SacII CC_GC'GG
Cuts at: 2042 2405 2042
Size: 363 6317
SalI G'TCGA_C
Cuts at: 3717 3717
Size: 6680
SanDI GG'GwC_CC
Cuts at: 3574 3574
Size: 6680
SapI GCTCTTCn'nnn_
Cuts at: 910 4703 4913 910
Size: 3793 210 2677
Fragments arranged by size:
3793 2677 210
SbfI CC_TGCA'GG
Cuts at: 3471 3727 3471
Size: 256 6424
ScaI AGT'ACT
Cuts at: 2992 6197 2992
Size: 3205 3475
SexAI A'CCwGG_T
Cuts at: 2141 3143 3482 2141
Size: 1002 339 5339
Fragments arranged by size:
5339 1002 339
SfcI C'TryA_G
Cuts at: 33 817 1421 1684 1905 1956 2650 3467 3723
Size: 784 604 263 221 51 694 817 256
Cuts at: 3723 4408 5401 5592 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 817 784 694 685 604 263
256 221 191 51
SfoI GGC'GCC
Cuts at: 3342 3357 3397 4112 4360 3342
Size: 15 40 715 248 5662
Fragments arranged by size:
5662 715 248 40 15
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6680
SmaI CCC'GGG
Cuts at: 1547 2197 2211 2573 3702 1547
Size: 650 14 362 1129 4525
Fragments arranged by size:
4525 1129 650 362 14
SmlI C'TyrA_G
Cuts at: 203 499 3835 4750 5242 5504 203
Size: 296 3336 915 492 262 1379
Fragments arranged by size:
3336 1379 915 492 296 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6680
SphI G_CATG'C
Cuts at: 3166 3733 4233 3166
Size: 567 500 5613
Fragments arranged by size:
5613 567 500
StuI AGG'CCT
Cuts at: 962 1201 1766 3218 962
Size: 239 565 1452 4424
Fragments arranged by size:
4424 1452 565 239
StyI C'CwwG_G
Cuts at: 107 219 314 767 1011 1042 1860 1920 3003
Size: 112 95 453 244 31 818 60 1083
Cuts at: 3003 3814 4168 107
Size: 811 354 2619
Fragments arranged by size:
2619 1083 818 811 453 354 244 112
95 60 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 570 1087 1116 1814 1951 2299 4263 4396 4855
Size: 517 29 698 137 348 1964 133 459
Cuts at: 4855 5064 570
Size: 209 2186
Fragments arranged by size:
2186 1964 698 517 459 348 209 137
133 29
TatI w'GTAC_w
Cuts at: 2990 6195 2990
Size: 3205 3475
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1789 1821 2783 2815 3440 3472 3514 3546 6197
Size: 32 962 32 625 32 42 32 2651
Cuts at: 6197 6229 1789
Size: 32 2240
Fragments arranged by size:
2651 2240 962 625 42 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 3145 4477 3145
Size: 1332 5348
XbaI T'CTAG_A
Cuts at: 3711 3711
Size: 6680
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2112 3182 4175 2112
Size: 1070 993 4617
Fragments arranged by size:
4617 1070 993
XhoI C'TCGA_G
Cuts at: 203 203
Size: 6680
XmaI C'CCGG_G
Cuts at: 1545 2195 2209 2571 3700 1545
Size: 650 14 362 1129 4525
Fragments arranged by size:
4525 1129 650 362 14
XmnI GAAnn'nnTTC
Cuts at: 3205 3205
Size: 6680
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AscI AvrII BaeI BbvCI
BfrBI BmgBI BmtI BsiWI BsmBI BsrGI BssHII BstEII
CspCI FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PshAI PsiI PspXI SfiI
SgrAI SnaBI SrfI SspI SwaI ZraI