Restriction map for
pF1KB3288
(Circular) (Six-base) MAPSORT of: pF1KB3288.seq Check: 6124 from: 1 to: 5345
pF1KB3288 5345 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 2087 2383 2087
Size: 296 5049
Acc65I G'GTAC_C
Cuts at: 1014 2361 1014
Size: 1347 3998
AclI AA'CG_TT
Cuts at: 5185 5185
Size: 5345
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3170 3602 3955 4617 3170
Size: 432 353 662 3898
Fragments arranged by size:
3898 662 432 353
AflIII A'CryG_T
Cuts at: 2126 2168 2697 4497 4778 2126
Size: 42 529 1800 281 2693
Fragments arranged by size:
2693 1800 529 281 42
AgeI A'CCGG_T
Cuts at: 1989 1989
Size: 5345
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 343 343
Size: 5345
AlwNI CAG_nnn'CTG
Cuts at: 138 267 2650 4088 138
Size: 129 2383 1438 1395
Fragments arranged by size:
2383 1438 1395 129
ApaI G_GGCC'C
Cuts at: 509 953 961 1315 1487 1594 1621 1685 2001
Size: 444 8 354 172 107 27 64 316
Cuts at: 2001 509
Size: 3853
Fragments arranged by size:
3853 444 354 316 172 107 64 27
8
ApaLI G'TGCA_C
Cuts at: 4183 4183
Size: 5345
ApoI r'AATT_y
Cuts at: 224 248 383 2349 4698 4954 224
Size: 24 135 1966 2349 256 615
Fragments arranged by size:
2349 1966 615 256 135 24
AseI AT'TA_AT
Cuts at: 20 20
Size: 5345
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5345
AvaI C'yCGr_G
Cuts at: 2365 2365
Size: 5345
BamHI G'GATC_C
Cuts at: 680 1073 2370 680
Size: 393 1297 3655
Fragments arranged by size:
3655 1297 393
BanI G'GyrC_C
Cuts at: 190 393 1014 1249 1750 2361 2775 3023 3058
Size: 203 621 235 501 611 414 248 35
Cuts at: 3058 190
Size: 2477
Fragments arranged by size:
2477 621 611 501 414 248 235 203
35
BanII G_rGCy'C
Cuts at: 509 953 961 981 1218 1315 1487 1594 1621
Size: 444 8 20 237 97 172 107 27
Cuts at: 1621 1685 2001 2159 2359 3389 509
Size: 64 316 158 200 1030 2465
Fragments arranged by size:
2465 1030 444 316 237 200 172 158
107 97 64 27 20 8
BbeI G_GCGC'C
Cuts at: 397 2779 3027 397
Size: 2382 248 2715
Fragments arranged by size:
2715 2382 248
BciVI GTATCCnnnnn_n'
Cuts at: 365 524 1034 1612 2342 3243 4299 5345 365
Size: 159 510 578 730 901 1056 1046 365
Fragments arranged by size:
1056 1046 901 730 578 510 365 159
BclI T'GATC_A
Cuts at: 2865 2865
Size: 5345
BglI GCCn_nnn'nGGC
Cuts at: 741 896 1374 1625 1775 1912 741
Size: 155 478 251 150 137 4174
Fragments arranged by size:
4174 478 251 155 150 137
BglII A'GATC_T
Cuts at: 2860 4897 2860
Size: 2037 3308
BlpI GC'TnA_GC
Cuts at: 2457 2457
Size: 5345
Bme1580I G_kGCm'C
Cuts at: 509 953 961 1254 1315 1487 1594 1621 1685
Size: 444 8 293 61 172 107 27 64
Cuts at: 1685 1755 2001 2970 3063 4187 509
Size: 70 246 969 93 1124 1667
Fragments arranged by size:
1667 1124 969 444 293 246 172 107
93 70 64 61 27 8
BmrI ACTGGGnnnn_n'
Cuts at: 1996 2557 2638 2973 5117 1996
Size: 561 81 335 2144 2224
Fragments arranged by size:
2224 2144 561 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3712 3712
Size: 5345
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 686 860 965 1256 1301 1784 1793 2239 686
Size: 174 105 291 45 483 9 446 3792
Fragments arranged by size:
3792 483 446 291 174 105 45 9
Bpu10I CC'TnA_GC
Cuts at: 2230 2230
Size: 5345
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 791 1748 2521 3400 4114 4412 791
Size: 957 773 879 714 298 1724
Fragments arranged by size:
1724 957 879 773 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2234 5049 2234
Size: 2815 2530
BsaAI yAC'GTr
Cuts at: 2171 2698 2171
Size: 527 4818
BsaBI GATnn'nnATC
Cuts at: 6 2859 4896 6
Size: 2853 2037 455
Fragments arranged by size:
2853 2037 455
BsaHI Gr'CG_yC
Cuts at: 394 2776 3024 3726 5222 394
Size: 2382 248 702 1496 517
Fragments arranged by size:
2382 1496 702 517 248
BsaWI w'CCGG_w
Cuts at: 1989 2534 3055 3733 4144 4291 4565 1989
Size: 545 521 678 411 147 274 2769
Fragments arranged by size:
2769 678 545 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1505 1535 2231 2261 1505
Size: 30 696 30 4589
Fragments arranged by size:
4589 696 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 221 2298 221
Size: 2077 3268
BseYI C'CCAG_C
Cuts at: 138 1498 1818 1875 2770 3528 4193 4601 138
Size: 1360 320 57 895 758 665 408 882
Fragments arranged by size:
1360 895 882 758 665 408 320 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2237 2237
Size: 5345
BsiEI CG_ry'CG
Cuts at: 104 2887 2933 4163 104
Size: 2783 46 1230 1286
Fragments arranged by size:
2783 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2359 3137 3327 4187 2359
Size: 778 190 860 3517
Fragments arranged by size:
3517 860 778 190
BsiWI C'GTAC_G
Cuts at: 2171 2171
Size: 5345
BsmI GAATG_Cn'
Cuts at: 605 605
Size: 5345
Bsp1286I G_dGCh'C
Cuts at: 509 953 961 981 1218 1254 1315 1487 1594
Size: 444 8 20 237 36 61 172 107
Cuts at: 1594 1621 1685 1755 2001 2159 2359 2970 3063
Size: 27 64 70 246 158 200 611 93
Cuts at: 3063 3137 3327 3389 4187 509
Size: 74 190 62 798 1667
Fragments arranged by size:
1667 798 611 444 246 237 200 190
172 158 107 93 74 70 64 62
61 36 27 20 8
BspHI T'CATG_A
Cuts at: 3777 3777
Size: 5345
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2395 2609 2911 3292 2395
Size: 214 302 381 4448
Fragments arranged by size:
4448 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3637 3691 5175 5340 50
Size: 3587 54 1484 165 55
Fragments arranged by size:
3587 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3257 3257
Size: 5345
BsrFI r'CCGG_y
Cuts at: 390 1989 3343 3524 390
Size: 1599 1354 181 2211
Fragments arranged by size:
2211 1599 1354 181
BsrGI T'GTAC_A
Cuts at: 2165 2165
Size: 5345
BssSI C'ACGA_G
Cuts at: 3616 4324 3616
Size: 708 4637
BstAPI GCAn_nnn'nTGC
Cuts at: 1119 2722 1119
Size: 1603 3742
BstBI TT'CG_AA
Cuts at: 3706 3706
Size: 5345
BstEII G'GTnAC_C
Cuts at: 749 1186 749
Size: 437 4908
BstXI CCAn_nnnn'nTGG
Cuts at: 2209 2209
Size: 5345
BstYI r'GATC_y
Cuts at: 1 680 1073 2370 2837 2860 3195 3441 3747
Size: 679 393 1297 467 23 335 246 306
Cuts at: 3747 3759 3845 4897 1
Size: 12 86 1052 449
Fragments arranged by size:
1297 1052 679 467 449 393 335 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1550 1550
Size: 5345
BtgI C'CryG_G
Cuts at: 107 314 474 620 2336 107
Size: 207 160 146 1716 3116
Fragments arranged by size:
3116 1716 207 160 146
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3295 3476 3639 3295
Size: 181 163 5001
Fragments arranged by size:
5001 181 163
BtsI GCAGTG_nn'
Cuts at: 2109 2225 2588 2109
Size: 116 363 4866
Fragments arranged by size:
4866 363 116
DraI TTT'AAA
Cuts at: 2344 2344
Size: 5345
DrdI GACnn_nn'nnGTC
Cuts at: 3051 4395 3051
Size: 1344 4001
EaeI y'GGCC_r
Cuts at: 296 2930 3104 3495 3522 4867 296
Size: 2634 174 391 27 1345 774
Fragments arranged by size:
2634 1345 774 391 174 27
EagI C'GGCC_G
Cuts at: 2930 2930
Size: 5345
EarI CTCTTCn'nnn_
Cuts at: 3368 3578 3368
Size: 210 5135
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3553 4295 4441 5155 3553
Size: 742 146 714 3743
Fragments arranged by size:
3743 742 714 146
EcoICRI GAG'CTC
Cuts at: 2357 2357
Size: 5345
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 686 860 965 1256 1301 1784 1793 2239 3170
Size: 174 105 291 45 483 9 446 931
Cuts at: 3170 3602 3955 4617 686
Size: 432 353 662 1414
Fragments arranged by size:
1414 931 662 483 446 432 353 291
174 105 45 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1700 2200 1700
Size: 500 4845
EcoO109I rG'GnC_Cy
Cuts at: 469 506 697 706 784 802 820 871 950
Size: 37 191 9 78 18 18 51 79
Cuts at: 950 957 958 1003 1030 1135 1177 1267 1303
Size: 7 1 45 27 105 42 90 36
Cuts at: 1303 1312 1384 1447 1474 1483 1590 1591 1617
Size: 9 72 63 27 9 107 1 26
Cuts at: 1617 1618 1654 1681 1682 1786 1795 1804 1813
Size: 1 36 27 1 104 9 9 9
Cuts at: 1813 1997 1998 2076 2484 469
Size: 184 1 78 408 3330
Fragments arranged by size:
3330 408 191 184 107 105 104 90
79 78 78 72 63 51 45 42
37 36 36 27 27 27 26 18
18 9 9 9 9 9 9 7
1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 2349 2349
Size: 5345
EcoRV GAT'ATC
Cuts at: 568 568
Size: 5345
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 325 3364 325
Size: 3039 2306
FspI TGC'GCA
Cuts at: 3126 3126
Size: 5345
HaeII r_GCGC'y
Cuts at: 397 2779 3027 4257 5028 397
Size: 2382 248 1230 771 714
Fragments arranged by size:
2382 1230 771 714 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2191 2223 3187 3217 3219 3249 2191
Size: 32 964 30 2 30 4287
Fragments arranged by size:
4287 964 32 30 30 2
HincII GTy'rAC
Cuts at: 2384 2384
Size: 5345
KasI G'GCGC_C
Cuts at: 393 2775 3023 393
Size: 2382 248 2715
Fragments arranged by size:
2715 2382 248
KpnI G_GTAC'C
Cuts at: 1018 2365 1018
Size: 1347 3998
MluI A'CGCG_T
Cuts at: 4778 4778
Size: 5345
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 558 885 4104 4288 558
Size: 327 3219 184 1615
Fragments arranged by size:
3219 1615 327 184
MscI TGG'CCA
Cuts at: 298 3106 298
Size: 2808 2537
MslI CAynn'nnrTG
Cuts at: 112 625 3334 3461 112
Size: 513 2709 127 1996
Fragments arranged by size:
2709 1996 513 127
MspA1I CmG'CkG
Cuts at: 135 213 1822 2456 2770 3130 3914 4159 135
Size: 78 1609 634 314 360 784 245 1321
Fragments arranged by size:
1609 1321 784 634 360 314 245 78
NaeI GCC'GGC
Cuts at: 3526 3526
Size: 5345
NarI GG'CG_CC
Cuts at: 394 2776 3024 394
Size: 2382 248 2715
Fragments arranged by size:
2715 2382 248
NcoI C'CATG_G
Cuts at: 107 314 620 2336 107
Size: 207 306 1716 3116
Fragments arranged by size:
3116 1716 306 207
NgoMIV G'CCGG_C
Cuts at: 3524 3524
Size: 5345
NspI r_CATG'y
Cuts at: 291 630 2398 2898 4501 291
Size: 339 1768 500 1603 1135
Fragments arranged by size:
1768 1603 1135 500 339
PasI CC'CwG_GG
Cuts at: 267 946 954 963 1570 1606 1678 1687 1738
Size: 679 8 9 607 36 72 9 51
Cuts at: 1738 1848 267
Size: 110 3764
Fragments arranged by size:
3764 679 607 110 72 51 36 9
9 8
PciI A'CATG_T
Cuts at: 4497 4497
Size: 5345
PflMI CCAn_nnn'nTGG
Cuts at: 267 526 577 1156 1612 267
Size: 259 51 579 456 4000
Fragments arranged by size:
4000 579 456 259 51
PfoI T'CCnGG_A
Cuts at: 570 981 1272 1335 1782 1800 1809 2195 570
Size: 411 291 63 447 18 9 386 3720
Fragments arranged by size:
3720 447 411 386 291 63 18 9
PmeI GTTT'AAAC
Cuts at: 2344 2344
Size: 5345
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3791 3823 3791
Size: 32 5313
PpuMI rG'GwC_Cy
Cuts at: 469 697 784 802 820 1030 1135 1177 1267
Size: 228 87 18 18 210 105 42 90
Cuts at: 1267 1303 1384 1474 1786 1804 1813 2076 469
Size: 36 81 90 312 18 9 263 3738
Fragments arranged by size:
3738 312 263 228 210 105 90 90
87 81 42 36 18 18 18 9
PspOMI G'GGCC_C
Cuts at: 505 949 957 1311 1483 1590 1617 1681 1997
Size: 444 8 354 172 107 27 64 316
Cuts at: 1997 505
Size: 3853
Fragments arranged by size:
3853 444 354 316 172 107 64 27
8
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1007 1039 1007
Size: 32 5313
PstI C_TGCA'G
Cuts at: 134 2280 2392 3077 134
Size: 2146 112 685 2402
Fragments arranged by size:
2402 2146 685 112
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5345
PvuII CAG'CTG
Cuts at: 135 1822 2770 3130 135
Size: 1687 948 360 2350
Fragments arranged by size:
2350 1687 948 360
RsrII CG'GwC_CG
Cuts at: 3540 3540
Size: 5345
SacI G_AGCT'C
Cuts at: 2359 2359
Size: 5345
SalI G'TCGA_C
Cuts at: 2382 2382
Size: 5345
SapI GCTCTTCn'nnn_
Cuts at: 3368 3578 3368
Size: 210 5135
SbfI CC_TGCA'GG
Cuts at: 2392 2392
Size: 5345
ScaI AGT'ACT
Cuts at: 4862 4862
Size: 5345
SexAI A'CCwGG_T
Cuts at: 1380 1380
Size: 5345
SfcI C'TryA_G
Cuts at: 33 130 2276 2388 3073 4041 4232 33
Size: 97 2146 112 685 968 191 1146
Fragments arranged by size:
2146 1146 968 685 191 112 97
SfiI GGCCn_nnn'nGGCC
Cuts at: 896 1374 1625 1775 896
Size: 478 251 150 4466
Fragments arranged by size:
4466 478 251 150
SfoI GGC'GCC
Cuts at: 395 2777 3025 395
Size: 2382 248 2715
Fragments arranged by size:
2715 2382 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5345
SgrAI Cr'CCGG_yG
Cuts at: 1989 1989
Size: 5345
SmaI CCC'GGG
Cuts at: 2367 2367
Size: 5345
SmlI C'TyrA_G
Cuts at: 806 1763 2500 3415 4129 4391 806
Size: 957 737 915 714 262 1760
Fragments arranged by size:
1760 957 915 737 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5345
SphI G_CATG'C
Cuts at: 630 2398 2898 630
Size: 1768 500 3077
Fragments arranged by size:
3077 1768 500
StuI AGG'CCT
Cuts at: 812 1145 1286 812
Size: 333 141 4871
Fragments arranged by size:
4871 333 141
StyI C'CwwG_G
Cuts at: 107 314 620 1388 1434 1487 1755 2336 2479
Size: 207 306 768 46 53 268 581 143
Cuts at: 2479 2833 107
Size: 354 2619
Fragments arranged by size:
2619 768 581 354 306 268 207 143
53 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2928 3061 3520 3729 2928
Size: 133 459 209 4544
Fragments arranged by size:
4544 459 209 133
TatI w'GTAC_w
Cuts at: 2165 2180 4860 2165
Size: 15 2680 2650
Fragments arranged by size:
2680 2650 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 723 755 837 869 1734 1766 2231 2263 4862
Size: 32 82 32 865 32 465 32 2599
Cuts at: 4862 4894 723
Size: 32 1174
Fragments arranged by size:
2599 1174 865 465 82 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 3142 3142
Size: 5345
XbaI T'CTAG_A
Cuts at: 2376 2376
Size: 5345
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 340 2840 340
Size: 2500 2845
XmaI C'CCGG_G
Cuts at: 2365 2365
Size: 5345
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
BsmBI BspEI BssHII BstZ17I ClaI CspCI DraIII FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PshAI PsiI PspXI SacII SanDI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI