Restriction map for pF1KB3300
 (Circular) (Six-base) MAPSORT of: pF1KB3300.seq  Check: 1667  from: 1  to: 5282



pF1KB3300  5282 bp

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2230    2230 
   Size:       5282
 
AccI GT'mk_AC

Cuts at:    665    2320     665 
   Size:       1655    3627
 
Acc65I G'GTAC_C

Cuts at:   2298    2298 
   Size:       5282
 
AclI AA'CG_TT

Cuts at:   5122    5122 
   Size:       5282
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    544     946     979    1111    3107    3539    4286    4554     544 
   Size:        402      33     132    1996     432     747     268    1272

  Fragments arranged by size: 

                1996    1272     747     432     402     268     132      33
            
 
AfeI AGC'GCT

Cuts at:   1630    1630 
   Size:       5282
 
AflIII A'CryG_T

Cuts at:   2634    3738    4715    2634 
   Size:       1104     977    3201

  Fragments arranged by size: 

                3201    1104     977
 
AgeI A'CCGG_T

Cuts at:   3670    3670 
   Size:       5282
 
AhdI GACnn_n'nnGTC

Cuts at:   1599    1599 
   Size:       5282
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    286     434    1487     286 
   Size:        148    1053    4081

  Fragments arranged by size: 

                4081    1053     148
 
AlwNI CAG_nnn'CTG

Cuts at:    612    1035    1131    1284    1401    1446    1659    1954    1992 
   Size:        423      96     153     117      45     213     295      38

Cuts at:   1992    2214    2587    4154     612 
   Size:        222     373    1567    1740

  Fragments arranged by size: 

                1740    1567     423     373     295     222     213     153
                 117      96      45      38
 
ApaI G_GGCC'C

Cuts at:    821    1638     821 
   Size:        817    4465
 
ApaLI G'TGCA_C

Cuts at:   4052    4052 
   Size:       5282
 
ApoI r'AATT_y

Cuts at:   2286    4635    4891    2286 
   Size:       2349     256    2677

  Fragments arranged by size: 

                2677    2349     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5282
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5282
 
AvaI C'yCGr_G

Cuts at:    800     910    2302     800 
   Size:        110    1392    3780

  Fragments arranged by size: 

                3780    1392     110
 
BamHI G'GATC_C

Cuts at:   1121    2009    2307    1121 
   Size:        888     298    4096

  Fragments arranged by size: 

                4096     888     298
 
BanI G'GyrC_C

Cuts at:    688     923    2298    2712    2960    2995     688 
   Size:        235    1375     414     248      35    2975

  Fragments arranged by size: 

                2975    1375     414     248     235      35
 
BanII G_rGCy'C

Cuts at:    230     821    1638    1694    1921    2296    3326     230 
   Size:        591     817      56     227     375    1030    2186

  Fragments arranged by size: 

                2186    1030     817     591     375     227      56
 
BbeI G_GCGC'C

Cuts at:    927    2716    2964     927 
   Size:       1789     248    3245

  Fragments arranged by size: 

                3245    1789     248
 
BbvCI CC'TCA_GC

Cuts at:   1639    1639 
   Size:       5282
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    5248
 
BciVI GTATCCnnnnn_n'

Cuts at:   3180    3941    5282    3180 
   Size:        761    1341    3180

  Fragments arranged by size: 

                3180    1341     761
 
BclI T'GATC_A

Cuts at:   2802    2802 
   Size:       5282
 
BfrBI ATG'CAT

Cuts at:   1509    1509 
   Size:       5282
 
BglII A'GATC_T

Cuts at:    366    2797    4834     366 
   Size:       2431    2037     814

  Fragments arranged by size: 

                2431    2037     814
 
BlpI GC'TnA_GC

Cuts at:    640    1856    1906    2394     640 
   Size:       1216      50     488    3528

  Fragments arranged by size: 

                3528    1216     488      50
 
Bme1580I G_kGCm'C

Cuts at:    821    1638    2907    3000    4056     821 
   Size:        817    1269      93    1056    2047

  Fragments arranged by size: 

                2047    1269    1056     817      93
 
BmgBI CAC'GTC

Cuts at:   1255    2184    1255 
   Size:        929    4353
 
BmrI ACTGGGnnnn_n'

Cuts at:    131     943    2494    2575    2910    5054     131 
   Size:        812    1551      81     335    2144     359

  Fragments arranged by size: 

                2144    1551     812     359     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1635    3649    1635 
   Size:       2014    3268
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    181     409     478     652     796     922    1045    1471    1591 
   Size:        228      69     174     144     126     123     426     120

Cuts at:   1591    1636    1861    2131     181 
   Size:         45     225     270    3332

  Fragments arranged by size: 

                3332     426     270     228     225     174     144     126
                 123     120      69      45
 
Bpu10I CC'TnA_GC

Cuts at:   1639    1639 
   Size:       5282
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    819    2458    3337    3829    4127     819 
   Size:       1639     879     492     298    1974

  Fragments arranged by size: 

                1974    1639     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4986    4986 
   Size:       5282
 
BsaAI yAC'GTr

Cuts at:   2635    2635 
   Size:       5282
 
BsaBI GATnn'nnATC

Cuts at:      6    2796    4833       6 
   Size:       2790    2037     455

  Fragments arranged by size: 

                2790    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    650     924    1943    2713    2961    3663    5159     650 
   Size:        274    1019     770     248     702    1496     773

  Fragments arranged by size: 

                1496    1019     773     770     702     274     248
 
BsaWI w'CCGG_w

Cuts at:    122    1976    2471    2992    3670    3944    4091    4502     122 
   Size:       1854     495     521     678     274     147     411     902

  Fragments arranged by size: 

                1854     902     678     521     495     411     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    835     865    1000    1030    1249    1279    1834    1864     835 
   Size:         30     135      30     219      30     555      30    4253

  Fragments arranged by size: 

                4253     555     219     135      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    157     856    1024    1246    1858     157 
   Size:        699     168     222     612    3581

  Fragments arranged by size: 

                3581     699     612     222     168
 
BseYI C'CCAG_C

Cuts at:    190     488     953    1631    2249    2707    3465    4042    4538 
   Size:        298     465     678     618     458     758     577     496

Cuts at:   4538     190 
   Size:        934

  Fragments arranged by size: 

                 934     758     678     618     577     496     465     458
                 298
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   2137    2137 
   Size:       5282
 
BsiEI CG_ry'CG

Cuts at:    104    2824    2870    4078     104 
   Size:       2720      46    1208    1308

  Fragments arranged by size: 

                2720    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    230     950    1046    1694    1862    2296    3074    3264    4056 
   Size:        720      96     648     168     434     778     190     792

Cuts at:   4056     230 
   Size:       1456

  Fragments arranged by size: 

                1456     792     778     720     648     434     190     168
                  96
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1950    1950 
   Size:       5282
 
Bsp1286I G_dGCh'C

Cuts at:    230     821     950    1046    1638    1694    1862    1921    2296 
   Size:        591     129      96     592      56     168      59     375

Cuts at:   2296    2907    3000    3074    3264    3326    4056     230 
   Size:        611      93      74     190      62     730    1456

  Fragments arranged by size: 

                1456     730     611     592     591     375     190     168
                 129      96      93      74      62      59      56
 
BspEI T'CCGG_A

Cuts at:    122    1976    4502     122 
   Size:       1854    2526     902

  Fragments arranged by size: 

                2526    1854     902
 
BspHI T'CATG_A

Cuts at:   4458    4458 
   Size:       5282
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1874    1910    2230    2332    2546    2848    3229    1874 
   Size:         36     320     102     214     302     381    3927

  Fragments arranged by size: 

                3927     381     320     302     214     102      36
 
BsrBI CCG'CTC

Cuts at:     50     114    1068    2095    3574    3628    5112    5277      50 
   Size:         64     954    1027    1479      54    1484     165      55

  Fragments arranged by size: 

                1484    1479    1027     954     165      64      55      54
            
 
BsrDI GCAATG_nn'

Cuts at:   3194    3194 
   Size:       5282
 
BsrFI r'CCGG_y

Cuts at:    691    1304    3280    3461    3670     691 
   Size:        613    1976     181     209    2303

  Fragments arranged by size: 

                2303    1976     613     209     181
 
BssSI C'ACGA_G

Cuts at:   1966    3553    3911    1966 
   Size:       1587     358    3337

  Fragments arranged by size: 

                3337    1587     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1281    1866    2659    1281 
   Size:        585     793    3904

  Fragments arranged by size: 

                3904     793     585
 
BstBI TT'CG_AA

Cuts at:    555     624    3643     555 
   Size:         69    3019    2194

  Fragments arranged by size: 

                3019    2194      69
 
BstYI r'GATC_y

Cuts at:      1     366     590     989    1121    1442    1970    2009    2307 
   Size:        365     224     399     132     321     528      39     298

Cuts at:   2307    2774    2797    3132    3378    4390    4476    4488    4834 
   Size:        467      23     335     246    1012      86      12     346

Cuts at:   4834       1 
   Size:        449

  Fragments arranged by size: 

                1012     528     467     449     399     365     346     335
                 321     298     246     224     132      86      39      23
                  12
 
BtgI C'CryG_G

Cuts at:    542     659     673     821     542 
   Size:        117      14     148    5003

  Fragments arranged by size: 

                5003     148     117      14
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1474    2229    3232    3413    3576    1474 
   Size:        755    1003     181     163    3180

  Fragments arranged by size: 

                3180    1003     755     181     163
 
BtsI GCAGTG_nn'

Cuts at:    695    2206    2212    2525     695 
   Size:       1511       6     313    3452

  Fragments arranged by size: 

                3452    1511     313       6
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    561     596     561 
   Size:         35    5247
 
DraI TTT'AAA

Cuts at:   2281    2281 
   Size:       5282
 
DrdI GACnn_nn'nnGTC

Cuts at:   2988    3846    2988 
   Size:        858    4424
 
EaeI y'GGCC_r

Cuts at:    656     701    1370    1931    2178    2867    3041    3432    3459 
   Size:         45     669     561     247     689     174     391      27

Cuts at:   3459    4804     656 
   Size:       1345    1134

  Fragments arranged by size: 

                1345    1134     689     669     561     391     247     174
                  45      27
 
EagI C'GGCC_G

Cuts at:   2867    2867 
   Size:       5282
 
EarI CTCTTCn'nnn_

Cuts at:    121     874    1682    1802    3305    3515     121 
   Size:        753     808     120    1503     210    1888

  Fragments arranged by size: 

                1888    1503     808     753     210     120
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1257    3490    3800    3946    5092    1257 
   Size:       2233     310     146    1146    1447

  Fragments arranged by size: 

                2233    1447    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    228    1692    2294     228 
   Size:       1464     602    3216

  Fragments arranged by size: 

                3216    1464     602
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    181     409     478     544     652     796     922     946     979 
   Size:        228      69      66     108     144     126      24      33

Cuts at:    979    1045    1111    1471    1591    1636    1861    2131    3107 
   Size:         66      66     360     120      45     225     270     976

Cuts at:   3107    3539    4286    4554     181 
   Size:        432     747     268     909

  Fragments arranged by size: 

                 976     909     747     432     360     270     268     228
                 225     144     126     120     108      69      66      66
                  66      45      33      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1840    1933    1840 
   Size:         93    5189
 
EcoO109I rG'GnC_Cy

Cuts at:   1032    1635    1926    2421    1032 
   Size:        603     291     495    3893

  Fragments arranged by size: 

                3893     603     495     291
 
EcoRI G'AATT_C

Cuts at:   2286    2286 
   Size:       5282
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3301    3301 
   Size:       5282
 
FspI TGC'GCA

Cuts at:   1880    2218    3063    1880 
   Size:        338     845    4099

  Fragments arranged by size: 

                4099     845     338
 
HaeII r_GCGC'y

Cuts at:    927    1053    1632    1824    2256    2716    2964    3986    4965 
   Size:        126     579     192     432     460     248    1022     979

Cuts at:   4965     927 
   Size:       1244

  Fragments arranged by size: 

                1244    1022     979     579     460     432     248     192
                 126
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    600     632     817     849    1000    1032    1249    1281    1585 
   Size:         32     185      32     151      32     217      32     304

Cuts at:   1585    1617    1746    1778    1834    1866    3124    3154    3156 
   Size:         32     129      32      56      32    1258      30       2

Cuts at:   3156    3186     600 
   Size:         30    2696

  Fragments arranged by size: 

                2696    1258     304     217     185     151     129      56
                  32      32      32      32      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:   2321    2321 
   Size:       5282
 
KasI G'GCGC_C

Cuts at:    923    2712    2960     923 
   Size:       1789     248    3245

  Fragments arranged by size: 

                3245    1789     248
 
KpnI G_GTAC'C

Cuts at:   2302    2302 
   Size:       5282
 
MluI A'CGCG_T

Cuts at:   4715    4715 
   Size:       5282
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3953    4137    3953 
   Size:        184    5098
 
MscI TGG'CCA

Cuts at:   1933    2180    3043    1933 
   Size:        247     863    4172

  Fragments arranged by size: 

                4172     863     247
 
MslI CAynn'nnrTG

Cuts at:   1315    3271    3398    1315 
   Size:       1956     127    3199

  Fragments arranged by size: 

                3199    1956     127
 
MspA1I CmG'CkG

Cuts at:    384     414     675     942    1089    1560    1569    1659    1827 
   Size:         30     261     267     147     471       9      90     168

Cuts at:   1827    2229    2393    2707    3067    4080    4325     384 
   Size:        402     164     314     360    1013     245    1341

  Fragments arranged by size: 

                1341    1013     471     402     360     314     267     261
                 245     168     164     147      90      30       9
 
NaeI GCC'GGC

Cuts at:    693    3463     693 
   Size:       2770    2512
 
NarI GG'CG_CC

Cuts at:    924    2713    2961     924 
   Size:       1789     248    3245

  Fragments arranged by size: 

                3245    1789     248
 
NcoI C'CATG_G

Cuts at:    659     659 
   Size:       5282
 
NgoMIV G'CCGG_C

Cuts at:    691    3461     691 
   Size:       2770    2512
 
NsiI A_TGCA'T

Cuts at:   1511    1511 
   Size:       5282
 
NspI r_CATG'y

Cuts at:    263    2335    2835    3742     263 
   Size:       2072     500     907    1803

  Fragments arranged by size: 

                2072    1803     907     500
 
PasI CC'CwG_GG

Cuts at:   1921    1921 
   Size:       5282
 
PciI A'CATG_T

Cuts at:   3738    3738 
   Size:       5282
 
PflMI CCAn_nnn'nTGG

Cuts at:   1830    1830 
   Size:       5282
 
PfoI T'CCnGG_A

Cuts at:   1607    1694    1838    1985    1607 
   Size:         87     144     147    4904

  Fragments arranged by size: 

                4904     147     144      87
 
PmeI GTTT'AAAC

Cuts at:   2281    2281 
   Size:       5282
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    850     882    4421    4453     850 
   Size:         32    3539      32    1679

  Fragments arranged by size: 

                3539    1679      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   1926    1926 
   Size:       5282
 
PspOMI G'GGCC_C

Cuts at:    817    1634     817 
   Size:        817    4465
 
PspXI vC'TCGA_Gb

Cuts at:    910     910 
   Size:       5282
 
PstI C_TGCA'G

Cuts at:    275     334    1082    1139    1451    1964    2106    2329    3014 
   Size:         59     748      57     312     513     142     223     685

Cuts at:   3014     275 
   Size:       2543

  Fragments arranged by size: 

                2543     748     685     513     312     223     142      59
                  57
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5282
 
PvuII CAG'CTG

Cuts at:    384     414    1089    1560    1569    1659    1827    2707    3067 
   Size:         30     675     471       9      90     168     880     360

Cuts at:   3067     384 
   Size:       2599

  Fragments arranged by size: 

                2599     880     675     471     360     168      90      30
                   9
 
RsrII CG'GwC_CG

Cuts at:   3477    3477 
   Size:       5282
 
SacI G_AGCT'C

Cuts at:    230    1694    2296     230 
   Size:       1464     602    3216

  Fragments arranged by size: 

                3216    1464     602
 
SacII CC_GC'GG

Cuts at:    676     676 
   Size:       5282
 
SalI G'TCGA_C

Cuts at:   2319    2319 
   Size:       5282
 
SapI GCTCTTCn'nnn_

Cuts at:    121    1682    3305    3515     121 
   Size:       1561    1623     210    1888

  Fragments arranged by size: 

                1888    1623    1561     210
 
SbfI CC_TGCA'GG

Cuts at:   1451    2329    1451 
   Size:        878    4404
 
ScaI AGT'ACT

Cuts at:    760    4799     760 
   Size:       4039    1243
 
SfcI C'TryA_G

Cuts at:     33     271     330    1078    1135    1447    1960    2102    2325 
   Size:        238      59     748      57     312     513     142     223

Cuts at:   2325    3010    4003    4194      33 
   Size:        685     993     191    1121

  Fragments arranged by size: 

                1121     993     748     685     513     312     238     223
                 191     142      59      57
 
SfoI GGC'GCC

Cuts at:    925    2714    2962     925 
   Size:       1789     248    3245

  Fragments arranged by size: 

                3245    1789     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5282
 
SmaI CCC'GGG

Cuts at:   2304    2304 
   Size:       5282
 
SmlI C'TyrA_G

Cuts at:    834     910    2437    3352    3844    4106     834 
   Size:         76    1527     915     492     262    2010

  Fragments arranged by size: 

                2010    1527     915     492     262      76
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5282
 
SphI G_CATG'C

Cuts at:   2335    2835    2335 
   Size:        500    4782
 
StuI AGG'CCT

Cuts at:   1357    2122    1357 
   Size:        765    4517
 
StyI C'CwwG_G

Cuts at:    659    1934    2416    2770     659 
   Size:       1275     482     354    3171

  Fragments arranged by size: 

                3171    1275     482     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    967    2026    2865    2998    3457    3666     967 
   Size:       1059     839     133     459     209    2583

  Fragments arranged by size: 

                2583    1059     839     459     209     133
 
TatI w'GTAC_w

Cuts at:    758    4797     758 
   Size:       4039    1243
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    123     155     295     327     835     867    4799    4831     123 
   Size:         32     140      32     508      32    3932      32     574

  Fragments arranged by size: 

                3932     574     508     140      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   3079    3079 
   Size:       5282
 
XbaI T'CTAG_A

Cuts at:    363    2313     363 
   Size:       1950    3332
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2777    2777 
   Size:       5282
 
XhoI C'TCGA_G

Cuts at:    910     910 
   Size:       5282
 
XmaI C'CCGG_G

Cuts at:   2302    2302 
   Size:       5282
 
XmnI GAAnn'nnTTC

Cuts at:    862     862 
   Size:       5282
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AhdI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI    BstYI     BtgI    BtgZI     BtsI    CspCI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NsiI     NspI     PasI     PciI    PflMI     PfoI     PmeI
     PpiI    PpuMI   PspOMI    PspXI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII    AflII     AleI     AloI     AscI    AvrII     BaeI     BbsI
     BglI     BmtI    BsiWI     BsmI    BsrGI   BssHII   BstEII    BstXI
  BstZ17I   Bsu36I     ClaI   DraIII    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     NdeI     NheI     NotI     NruI     PacI     PmlI
    PshAI     PsiI     PsrI    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     SspI     SwaI     ZraI
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