(Circular) (Six-base) MAPSORT of: pF1KB3300.seq Check: 1667 from: 1 to: 5282 pF1KB3300 5282 bp With 182 enzymes: SgfI * September 18, 2009 17:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2230 2230 Size: 5282 AccI GT'mk_AC Cuts at: 665 2320 665 Size: 1655 3627 Acc65I G'GTAC_C Cuts at: 2298 2298 Size: 5282 AclI AA'CG_TT Cuts at: 5122 5122 Size: 5282 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 544 946 979 1111 3107 3539 4286 4554 544 Size: 402 33 132 1996 432 747 268 1272 Fragments arranged by size: 1996 1272 747 432 402 268 132 33 AfeI AGC'GCT Cuts at: 1630 1630 Size: 5282 AflIII A'CryG_T Cuts at: 2634 3738 4715 2634 Size: 1104 977 3201 Fragments arranged by size: 3201 1104 977 AgeI A'CCGG_T Cuts at: 3670 3670 Size: 5282 AhdI GACnn_n'nnGTC Cuts at: 1599 1599 Size: 5282 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 286 434 1487 286 Size: 148 1053 4081 Fragments arranged by size: 4081 1053 148 AlwNI CAG_nnn'CTG Cuts at: 612 1035 1131 1284 1401 1446 1659 1954 1992 Size: 423 96 153 117 45 213 295 38 Cuts at: 1992 2214 2587 4154 612 Size: 222 373 1567 1740 Fragments arranged by size: 1740 1567 423 373 295 222 213 153 117 96 45 38 ApaI G_GGCC'C Cuts at: 821 1638 821 Size: 817 4465 ApaLI G'TGCA_C Cuts at: 4052 4052 Size: 5282 ApoI r'AATT_y Cuts at: 2286 4635 4891 2286 Size: 2349 256 2677 Fragments arranged by size: 2677 2349 256 AseI AT'TA_AT Cuts at: 20 20 Size: 5282 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5282 AvaI C'yCGr_G Cuts at: 800 910 2302 800 Size: 110 1392 3780 Fragments arranged by size: 3780 1392 110 BamHI G'GATC_C Cuts at: 1121 2009 2307 1121 Size: 888 298 4096 Fragments arranged by size: 4096 888 298 BanI G'GyrC_C Cuts at: 688 923 2298 2712 2960 2995 688 Size: 235 1375 414 248 35 2975 Fragments arranged by size: 2975 1375 414 248 235 35 BanII G_rGCy'C Cuts at: 230 821 1638 1694 1921 2296 3326 230 Size: 591 817 56 227 375 1030 2186 Fragments arranged by size: 2186 1030 817 591 375 227 56 BbeI G_GCGC'C Cuts at: 927 2716 2964 927 Size: 1789 248 3245 Fragments arranged by size: 3245 1789 248 BbvCI CC'TCA_GC Cuts at: 1639 1639 Size: 5282 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 90 Size: 34 5248 BciVI GTATCCnnnnn_n' Cuts at: 3180 3941 5282 3180 Size: 761 1341 3180 Fragments arranged by size: 3180 1341 761 BclI T'GATC_A Cuts at: 2802 2802 Size: 5282 BfrBI ATG'CAT Cuts at: 1509 1509 Size: 5282 BglII A'GATC_T Cuts at: 366 2797 4834 366 Size: 2431 2037 814 Fragments arranged by size: 2431 2037 814 BlpI GC'TnA_GC Cuts at: 640 1856 1906 2394 640 Size: 1216 50 488 3528 Fragments arranged by size: 3528 1216 488 50 Bme1580I G_kGCm'C Cuts at: 821 1638 2907 3000 4056 821 Size: 817 1269 93 1056 2047 Fragments arranged by size: 2047 1269 1056 817 93 BmgBI CAC'GTC Cuts at: 1255 2184 1255 Size: 929 4353 BmrI ACTGGGnnnn_n' Cuts at: 131 943 2494 2575 2910 5054 131 Size: 812 1551 81 335 2144 359 Fragments arranged by size: 2144 1551 812 359 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1635 3649 1635 Size: 2014 3268 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 181 409 478 652 796 922 1045 1471 1591 Size: 228 69 174 144 126 123 426 120 Cuts at: 1591 1636 1861 2131 181 Size: 45 225 270 3332 Fragments arranged by size: 3332 426 270 228 225 174 144 126 123 120 69 45 Bpu10I CC'TnA_GC Cuts at: 1639 1639 Size: 5282 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 819 2458 3337 3829 4127 819 Size: 1639 879 492 298 1974 Fragments arranged by size: 1974 1639 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4986 4986 Size: 5282 BsaAI yAC'GTr Cuts at: 2635 2635 Size: 5282 BsaBI GATnn'nnATC Cuts at: 6 2796 4833 6 Size: 2790 2037 455 Fragments arranged by size: 2790 2037 455 BsaHI Gr'CG_yC Cuts at: 650 924 1943 2713 2961 3663 5159 650 Size: 274 1019 770 248 702 1496 773 Fragments arranged by size: 1496 1019 773 770 702 274 248 BsaWI w'CCGG_w Cuts at: 122 1976 2471 2992 3670 3944 4091 4502 122 Size: 1854 495 521 678 274 147 411 902 Fragments arranged by size: 1854 902 678 521 495 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 835 865 1000 1030 1249 1279 1834 1864 835 Size: 30 135 30 219 30 555 30 4253 Fragments arranged by size: 4253 555 219 135 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 157 856 1024 1246 1858 157 Size: 699 168 222 612 3581 Fragments arranged by size: 3581 699 612 222 168 BseYI C'CCAG_C Cuts at: 190 488 953 1631 2249 2707 3465 4042 4538 Size: 298 465 678 618 458 758 577 496 Cuts at: 4538 190 Size: 934 Fragments arranged by size: 934 758 678 618 577 496 465 458 298 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 2137 2137 Size: 5282 BsiEI CG_ry'CG Cuts at: 104 2824 2870 4078 104 Size: 2720 46 1208 1308 Fragments arranged by size: 2720 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 230 950 1046 1694 1862 2296 3074 3264 4056 Size: 720 96 648 168 434 778 190 792 Cuts at: 4056 230 Size: 1456 Fragments arranged by size: 1456 792 778 720 648 434 190 168 96 BsmBI CGTCTCn'nnnn_ Cuts at: 1950 1950 Size: 5282 Bsp1286I G_dGCh'C Cuts at: 230 821 950 1046 1638 1694 1862 1921 2296 Size: 591 129 96 592 56 168 59 375 Cuts at: 2296 2907 3000 3074 3264 3326 4056 230 Size: 611 93 74 190 62 730 1456 Fragments arranged by size: 1456 730 611 592 591 375 190 168 129 96 93 74 62 59 56 BspEI T'CCGG_A Cuts at: 122 1976 4502 122 Size: 1854 2526 902 Fragments arranged by size: 2526 1854 902 BspHI T'CATG_A Cuts at: 4458 4458 Size: 5282 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1874 1910 2230 2332 2546 2848 3229 1874 Size: 36 320 102 214 302 381 3927 Fragments arranged by size: 3927 381 320 302 214 102 36 BsrBI CCG'CTC Cuts at: 50 114 1068 2095 3574 3628 5112 5277 50 Size: 64 954 1027 1479 54 1484 165 55 Fragments arranged by size: 1484 1479 1027 954 165 64 55 54 BsrDI GCAATG_nn' Cuts at: 3194 3194 Size: 5282 BsrFI r'CCGG_y Cuts at: 691 1304 3280 3461 3670 691 Size: 613 1976 181 209 2303 Fragments arranged by size: 2303 1976 613 209 181 BssSI C'ACGA_G Cuts at: 1966 3553 3911 1966 Size: 1587 358 3337 Fragments arranged by size: 3337 1587 358 BstAPI GCAn_nnn'nTGC Cuts at: 1281 1866 2659 1281 Size: 585 793 3904 Fragments arranged by size: 3904 793 585 BstBI TT'CG_AA Cuts at: 555 624 3643 555 Size: 69 3019 2194 Fragments arranged by size: 3019 2194 69 BstYI r'GATC_y Cuts at: 1 366 590 989 1121 1442 1970 2009 2307 Size: 365 224 399 132 321 528 39 298 Cuts at: 2307 2774 2797 3132 3378 4390 4476 4488 4834 Size: 467 23 335 246 1012 86 12 346 Cuts at: 4834 1 Size: 449 Fragments arranged by size: 1012 528 467 449 399 365 346 335 321 298 246 224 132 86 39 23 12 BtgI C'CryG_G Cuts at: 542 659 673 821 542 Size: 117 14 148 5003 Fragments arranged by size: 5003 148 117 14 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1474 2229 3232 3413 3576 1474 Size: 755 1003 181 163 3180 Fragments arranged by size: 3180 1003 755 181 163 BtsI GCAGTG_nn' Cuts at: 695 2206 2212 2525 695 Size: 1511 6 313 3452 Fragments arranged by size: 3452 1511 313 6 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 561 596 561 Size: 35 5247 DraI TTT'AAA Cuts at: 2281 2281 Size: 5282 DrdI GACnn_nn'nnGTC Cuts at: 2988 3846 2988 Size: 858 4424 EaeI y'GGCC_r Cuts at: 656 701 1370 1931 2178 2867 3041 3432 3459 Size: 45 669 561 247 689 174 391 27 Cuts at: 3459 4804 656 Size: 1345 1134 Fragments arranged by size: 1345 1134 689 669 561 391 247 174 45 27 EagI C'GGCC_G Cuts at: 2867 2867 Size: 5282 EarI CTCTTCn'nnn_ Cuts at: 121 874 1682 1802 3305 3515 121 Size: 753 808 120 1503 210 1888 Fragments arranged by size: 1888 1503 808 753 210 120 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1257 3490 3800 3946 5092 1257 Size: 2233 310 146 1146 1447 Fragments arranged by size: 2233 1447 1146 310 146 EcoICRI GAG'CTC Cuts at: 228 1692 2294 228 Size: 1464 602 3216 Fragments arranged by size: 3216 1464 602 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 181 409 478 544 652 796 922 946 979 Size: 228 69 66 108 144 126 24 33 Cuts at: 979 1045 1111 1471 1591 1636 1861 2131 3107 Size: 66 66 360 120 45 225 270 976 Cuts at: 3107 3539 4286 4554 181 Size: 432 747 268 909 Fragments arranged by size: 976 909 747 432 360 270 268 228 225 144 126 120 108 69 66 66 66 45 33 24 EcoNI CCTnn'n_nnAGG Cuts at: 1840 1933 1840 Size: 93 5189 EcoO109I rG'GnC_Cy Cuts at: 1032 1635 1926 2421 1032 Size: 603 291 495 3893 Fragments arranged by size: 3893 603 495 291 EcoRI G'AATT_C Cuts at: 2286 2286 Size: 5282 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3301 3301 Size: 5282 FspI TGC'GCA Cuts at: 1880 2218 3063 1880 Size: 338 845 4099 Fragments arranged by size: 4099 845 338 HaeII r_GCGC'y Cuts at: 927 1053 1632 1824 2256 2716 2964 3986 4965 Size: 126 579 192 432 460 248 1022 979 Cuts at: 4965 927 Size: 1244 Fragments arranged by size: 1244 1022 979 579 460 432 248 192 126 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 600 632 817 849 1000 1032 1249 1281 1585 Size: 32 185 32 151 32 217 32 304 Cuts at: 1585 1617 1746 1778 1834 1866 3124 3154 3156 Size: 32 129 32 56 32 1258 30 2 Cuts at: 3156 3186 600 Size: 30 2696 Fragments arranged by size: 2696 1258 304 217 185 151 129 56 32 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 2321 2321 Size: 5282 KasI G'GCGC_C Cuts at: 923 2712 2960 923 Size: 1789 248 3245 Fragments arranged by size: 3245 1789 248 KpnI G_GTAC'C Cuts at: 2302 2302 Size: 5282 MluI A'CGCG_T Cuts at: 4715 4715 Size: 5282 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3953 4137 3953 Size: 184 5098 MscI TGG'CCA Cuts at: 1933 2180 3043 1933 Size: 247 863 4172 Fragments arranged by size: 4172 863 247 MslI CAynn'nnrTG Cuts at: 1315 3271 3398 1315 Size: 1956 127 3199 Fragments arranged by size: 3199 1956 127 MspA1I CmG'CkG Cuts at: 384 414 675 942 1089 1560 1569 1659 1827 Size: 30 261 267 147 471 9 90 168 Cuts at: 1827 2229 2393 2707 3067 4080 4325 384 Size: 402 164 314 360 1013 245 1341 Fragments arranged by size: 1341 1013 471 402 360 314 267 261 245 168 164 147 90 30 9 NaeI GCC'GGC Cuts at: 693 3463 693 Size: 2770 2512 NarI GG'CG_CC Cuts at: 924 2713 2961 924 Size: 1789 248 3245 Fragments arranged by size: 3245 1789 248 NcoI C'CATG_G Cuts at: 659 659 Size: 5282 NgoMIV G'CCGG_C Cuts at: 691 3461 691 Size: 2770 2512 NsiI A_TGCA'T Cuts at: 1511 1511 Size: 5282 NspI r_CATG'y Cuts at: 263 2335 2835 3742 263 Size: 2072 500 907 1803 Fragments arranged by size: 2072 1803 907 500 PasI CC'CwG_GG Cuts at: 1921 1921 Size: 5282 PciI A'CATG_T Cuts at: 3738 3738 Size: 5282 PflMI CCAn_nnn'nTGG Cuts at: 1830 1830 Size: 5282 PfoI T'CCnGG_A Cuts at: 1607 1694 1838 1985 1607 Size: 87 144 147 4904 Fragments arranged by size: 4904 147 144 87 PmeI GTTT'AAAC Cuts at: 2281 2281 Size: 5282 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 850 882 4421 4453 850 Size: 32 3539 32 1679 Fragments arranged by size: 3539 1679 32 32 PpuMI rG'GwC_Cy Cuts at: 1926 1926 Size: 5282 PspOMI G'GGCC_C Cuts at: 817 1634 817 Size: 817 4465 PspXI vC'TCGA_Gb Cuts at: 910 910 Size: 5282 PstI C_TGCA'G Cuts at: 275 334 1082 1139 1451 1964 2106 2329 3014 Size: 59 748 57 312 513 142 223 685 Cuts at: 3014 275 Size: 2543 Fragments arranged by size: 2543 748 685 513 312 223 142 59 57 PvuI CG_AT'CG Cuts at: 104 104 Size: 5282 PvuII CAG'CTG Cuts at: 384 414 1089 1560 1569 1659 1827 2707 3067 Size: 30 675 471 9 90 168 880 360 Cuts at: 3067 384 Size: 2599 Fragments arranged by size: 2599 880 675 471 360 168 90 30 9 RsrII CG'GwC_CG Cuts at: 3477 3477 Size: 5282 SacI G_AGCT'C Cuts at: 230 1694 2296 230 Size: 1464 602 3216 Fragments arranged by size: 3216 1464 602 SacII CC_GC'GG Cuts at: 676 676 Size: 5282 SalI G'TCGA_C Cuts at: 2319 2319 Size: 5282 SapI GCTCTTCn'nnn_ Cuts at: 121 1682 3305 3515 121 Size: 1561 1623 210 1888 Fragments arranged by size: 1888 1623 1561 210 SbfI CC_TGCA'GG Cuts at: 1451 2329 1451 Size: 878 4404 ScaI AGT'ACT Cuts at: 760 4799 760 Size: 4039 1243 SfcI C'TryA_G Cuts at: 33 271 330 1078 1135 1447 1960 2102 2325 Size: 238 59 748 57 312 513 142 223 Cuts at: 2325 3010 4003 4194 33 Size: 685 993 191 1121 Fragments arranged by size: 1121 993 748 685 513 312 238 223 191 142 59 57 SfoI GGC'GCC Cuts at: 925 2714 2962 925 Size: 1789 248 3245 Fragments arranged by size: 3245 1789 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5282 SmaI CCC'GGG Cuts at: 2304 2304 Size: 5282 SmlI C'TyrA_G Cuts at: 834 910 2437 3352 3844 4106 834 Size: 76 1527 915 492 262 2010 Fragments arranged by size: 2010 1527 915 492 262 76 SpeI A'CTAG_T Cuts at: 67 67 Size: 5282 SphI G_CATG'C Cuts at: 2335 2835 2335 Size: 500 4782 StuI AGG'CCT Cuts at: 1357 2122 1357 Size: 765 4517 StyI C'CwwG_G Cuts at: 659 1934 2416 2770 659 Size: 1275 482 354 3171 Fragments arranged by size: 3171 1275 482 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 967 2026 2865 2998 3457 3666 967 Size: 1059 839 133 459 209 2583 Fragments arranged by size: 2583 1059 839 459 209 133 TatI w'GTAC_w Cuts at: 758 4797 758 Size: 4039 1243 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 123 155 295 327 835 867 4799 4831 123 Size: 32 140 32 508 32 3932 32 574 Fragments arranged by size: 3932 574 508 140 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 3079 3079 Size: 5282 XbaI T'CTAG_A Cuts at: 363 2313 363 Size: 1950 3332 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2777 2777 Size: 5282 XhoI C'TCGA_G Cuts at: 910 910 Size: 5282 XmaI C'CCGG_G Cuts at: 2302 2302 Size: 5282 XmnI GAAnn'nnTTC Cuts at: 862 862 Size: 5282 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII AflII AleI AloI AscI AvrII BaeI BbsI BglI BmtI BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI PacI PmlI PshAI PsiI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI ZraI