Restriction map for
pF1KB3300
(Circular) (Six-base) MAPSORT of: pF1KB3300.seq Check: 1667 from: 1 to: 5282
pF1KB3300 5282 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2230 2230
Size: 5282
AccI GT'mk_AC
Cuts at: 665 2320 665
Size: 1655 3627
Acc65I G'GTAC_C
Cuts at: 2298 2298
Size: 5282
AclI AA'CG_TT
Cuts at: 5122 5122
Size: 5282
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 946 979 1111 3107 3539 4286 4554 544
Size: 402 33 132 1996 432 747 268 1272
Fragments arranged by size:
1996 1272 747 432 402 268 132 33
AfeI AGC'GCT
Cuts at: 1630 1630
Size: 5282
AflIII A'CryG_T
Cuts at: 2634 3738 4715 2634
Size: 1104 977 3201
Fragments arranged by size:
3201 1104 977
AgeI A'CCGG_T
Cuts at: 3670 3670
Size: 5282
AhdI GACnn_n'nnGTC
Cuts at: 1599 1599
Size: 5282
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 286 434 1487 286
Size: 148 1053 4081
Fragments arranged by size:
4081 1053 148
AlwNI CAG_nnn'CTG
Cuts at: 612 1035 1131 1284 1401 1446 1659 1954 1992
Size: 423 96 153 117 45 213 295 38
Cuts at: 1992 2214 2587 4154 612
Size: 222 373 1567 1740
Fragments arranged by size:
1740 1567 423 373 295 222 213 153
117 96 45 38
ApaI G_GGCC'C
Cuts at: 821 1638 821
Size: 817 4465
ApaLI G'TGCA_C
Cuts at: 4052 4052
Size: 5282
ApoI r'AATT_y
Cuts at: 2286 4635 4891 2286
Size: 2349 256 2677
Fragments arranged by size:
2677 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5282
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5282
AvaI C'yCGr_G
Cuts at: 800 910 2302 800
Size: 110 1392 3780
Fragments arranged by size:
3780 1392 110
BamHI G'GATC_C
Cuts at: 1121 2009 2307 1121
Size: 888 298 4096
Fragments arranged by size:
4096 888 298
BanI G'GyrC_C
Cuts at: 688 923 2298 2712 2960 2995 688
Size: 235 1375 414 248 35 2975
Fragments arranged by size:
2975 1375 414 248 235 35
BanII G_rGCy'C
Cuts at: 230 821 1638 1694 1921 2296 3326 230
Size: 591 817 56 227 375 1030 2186
Fragments arranged by size:
2186 1030 817 591 375 227 56
BbeI G_GCGC'C
Cuts at: 927 2716 2964 927
Size: 1789 248 3245
Fragments arranged by size:
3245 1789 248
BbvCI CC'TCA_GC
Cuts at: 1639 1639
Size: 5282
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5248
BciVI GTATCCnnnnn_n'
Cuts at: 3180 3941 5282 3180
Size: 761 1341 3180
Fragments arranged by size:
3180 1341 761
BclI T'GATC_A
Cuts at: 2802 2802
Size: 5282
BfrBI ATG'CAT
Cuts at: 1509 1509
Size: 5282
BglII A'GATC_T
Cuts at: 366 2797 4834 366
Size: 2431 2037 814
Fragments arranged by size:
2431 2037 814
BlpI GC'TnA_GC
Cuts at: 640 1856 1906 2394 640
Size: 1216 50 488 3528
Fragments arranged by size:
3528 1216 488 50
Bme1580I G_kGCm'C
Cuts at: 821 1638 2907 3000 4056 821
Size: 817 1269 93 1056 2047
Fragments arranged by size:
2047 1269 1056 817 93
BmgBI CAC'GTC
Cuts at: 1255 2184 1255
Size: 929 4353
BmrI ACTGGGnnnn_n'
Cuts at: 131 943 2494 2575 2910 5054 131
Size: 812 1551 81 335 2144 359
Fragments arranged by size:
2144 1551 812 359 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1635 3649 1635
Size: 2014 3268
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 409 478 652 796 922 1045 1471 1591
Size: 228 69 174 144 126 123 426 120
Cuts at: 1591 1636 1861 2131 181
Size: 45 225 270 3332
Fragments arranged by size:
3332 426 270 228 225 174 144 126
123 120 69 45
Bpu10I CC'TnA_GC
Cuts at: 1639 1639
Size: 5282
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 819 2458 3337 3829 4127 819
Size: 1639 879 492 298 1974
Fragments arranged by size:
1974 1639 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4986 4986
Size: 5282
BsaAI yAC'GTr
Cuts at: 2635 2635
Size: 5282
BsaBI GATnn'nnATC
Cuts at: 6 2796 4833 6
Size: 2790 2037 455
Fragments arranged by size:
2790 2037 455
BsaHI Gr'CG_yC
Cuts at: 650 924 1943 2713 2961 3663 5159 650
Size: 274 1019 770 248 702 1496 773
Fragments arranged by size:
1496 1019 773 770 702 274 248
BsaWI w'CCGG_w
Cuts at: 122 1976 2471 2992 3670 3944 4091 4502 122
Size: 1854 495 521 678 274 147 411 902
Fragments arranged by size:
1854 902 678 521 495 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 835 865 1000 1030 1249 1279 1834 1864 835
Size: 30 135 30 219 30 555 30 4253
Fragments arranged by size:
4253 555 219 135 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 157 856 1024 1246 1858 157
Size: 699 168 222 612 3581
Fragments arranged by size:
3581 699 612 222 168
BseYI C'CCAG_C
Cuts at: 190 488 953 1631 2249 2707 3465 4042 4538
Size: 298 465 678 618 458 758 577 496
Cuts at: 4538 190
Size: 934
Fragments arranged by size:
934 758 678 618 577 496 465 458
298
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2137 2137
Size: 5282
BsiEI CG_ry'CG
Cuts at: 104 2824 2870 4078 104
Size: 2720 46 1208 1308
Fragments arranged by size:
2720 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 230 950 1046 1694 1862 2296 3074 3264 4056
Size: 720 96 648 168 434 778 190 792
Cuts at: 4056 230
Size: 1456
Fragments arranged by size:
1456 792 778 720 648 434 190 168
96
BsmBI CGTCTCn'nnnn_
Cuts at: 1950 1950
Size: 5282
Bsp1286I G_dGCh'C
Cuts at: 230 821 950 1046 1638 1694 1862 1921 2296
Size: 591 129 96 592 56 168 59 375
Cuts at: 2296 2907 3000 3074 3264 3326 4056 230
Size: 611 93 74 190 62 730 1456
Fragments arranged by size:
1456 730 611 592 591 375 190 168
129 96 93 74 62 59 56
BspEI T'CCGG_A
Cuts at: 122 1976 4502 122
Size: 1854 2526 902
Fragments arranged by size:
2526 1854 902
BspHI T'CATG_A
Cuts at: 4458 4458
Size: 5282
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1874 1910 2230 2332 2546 2848 3229 1874
Size: 36 320 102 214 302 381 3927
Fragments arranged by size:
3927 381 320 302 214 102 36
BsrBI CCG'CTC
Cuts at: 50 114 1068 2095 3574 3628 5112 5277 50
Size: 64 954 1027 1479 54 1484 165 55
Fragments arranged by size:
1484 1479 1027 954 165 64 55 54
BsrDI GCAATG_nn'
Cuts at: 3194 3194
Size: 5282
BsrFI r'CCGG_y
Cuts at: 691 1304 3280 3461 3670 691
Size: 613 1976 181 209 2303
Fragments arranged by size:
2303 1976 613 209 181
BssSI C'ACGA_G
Cuts at: 1966 3553 3911 1966
Size: 1587 358 3337
Fragments arranged by size:
3337 1587 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1281 1866 2659 1281
Size: 585 793 3904
Fragments arranged by size:
3904 793 585
BstBI TT'CG_AA
Cuts at: 555 624 3643 555
Size: 69 3019 2194
Fragments arranged by size:
3019 2194 69
BstYI r'GATC_y
Cuts at: 1 366 590 989 1121 1442 1970 2009 2307
Size: 365 224 399 132 321 528 39 298
Cuts at: 2307 2774 2797 3132 3378 4390 4476 4488 4834
Size: 467 23 335 246 1012 86 12 346
Cuts at: 4834 1
Size: 449
Fragments arranged by size:
1012 528 467 449 399 365 346 335
321 298 246 224 132 86 39 23
12
BtgI C'CryG_G
Cuts at: 542 659 673 821 542
Size: 117 14 148 5003
Fragments arranged by size:
5003 148 117 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1474 2229 3232 3413 3576 1474
Size: 755 1003 181 163 3180
Fragments arranged by size:
3180 1003 755 181 163
BtsI GCAGTG_nn'
Cuts at: 695 2206 2212 2525 695
Size: 1511 6 313 3452
Fragments arranged by size:
3452 1511 313 6
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 561 596 561
Size: 35 5247
DraI TTT'AAA
Cuts at: 2281 2281
Size: 5282
DrdI GACnn_nn'nnGTC
Cuts at: 2988 3846 2988
Size: 858 4424
EaeI y'GGCC_r
Cuts at: 656 701 1370 1931 2178 2867 3041 3432 3459
Size: 45 669 561 247 689 174 391 27
Cuts at: 3459 4804 656
Size: 1345 1134
Fragments arranged by size:
1345 1134 689 669 561 391 247 174
45 27
EagI C'GGCC_G
Cuts at: 2867 2867
Size: 5282
EarI CTCTTCn'nnn_
Cuts at: 121 874 1682 1802 3305 3515 121
Size: 753 808 120 1503 210 1888
Fragments arranged by size:
1888 1503 808 753 210 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1257 3490 3800 3946 5092 1257
Size: 2233 310 146 1146 1447
Fragments arranged by size:
2233 1447 1146 310 146
EcoICRI GAG'CTC
Cuts at: 228 1692 2294 228
Size: 1464 602 3216
Fragments arranged by size:
3216 1464 602
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 409 478 544 652 796 922 946 979
Size: 228 69 66 108 144 126 24 33
Cuts at: 979 1045 1111 1471 1591 1636 1861 2131 3107
Size: 66 66 360 120 45 225 270 976
Cuts at: 3107 3539 4286 4554 181
Size: 432 747 268 909
Fragments arranged by size:
976 909 747 432 360 270 268 228
225 144 126 120 108 69 66 66
66 45 33 24
EcoNI CCTnn'n_nnAGG
Cuts at: 1840 1933 1840
Size: 93 5189
EcoO109I rG'GnC_Cy
Cuts at: 1032 1635 1926 2421 1032
Size: 603 291 495 3893
Fragments arranged by size:
3893 603 495 291
EcoRI G'AATT_C
Cuts at: 2286 2286
Size: 5282
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3301 3301
Size: 5282
FspI TGC'GCA
Cuts at: 1880 2218 3063 1880
Size: 338 845 4099
Fragments arranged by size:
4099 845 338
HaeII r_GCGC'y
Cuts at: 927 1053 1632 1824 2256 2716 2964 3986 4965
Size: 126 579 192 432 460 248 1022 979
Cuts at: 4965 927
Size: 1244
Fragments arranged by size:
1244 1022 979 579 460 432 248 192
126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 600 632 817 849 1000 1032 1249 1281 1585
Size: 32 185 32 151 32 217 32 304
Cuts at: 1585 1617 1746 1778 1834 1866 3124 3154 3156
Size: 32 129 32 56 32 1258 30 2
Cuts at: 3156 3186 600
Size: 30 2696
Fragments arranged by size:
2696 1258 304 217 185 151 129 56
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2321 2321
Size: 5282
KasI G'GCGC_C
Cuts at: 923 2712 2960 923
Size: 1789 248 3245
Fragments arranged by size:
3245 1789 248
KpnI G_GTAC'C
Cuts at: 2302 2302
Size: 5282
MluI A'CGCG_T
Cuts at: 4715 4715
Size: 5282
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3953 4137 3953
Size: 184 5098
MscI TGG'CCA
Cuts at: 1933 2180 3043 1933
Size: 247 863 4172
Fragments arranged by size:
4172 863 247
MslI CAynn'nnrTG
Cuts at: 1315 3271 3398 1315
Size: 1956 127 3199
Fragments arranged by size:
3199 1956 127
MspA1I CmG'CkG
Cuts at: 384 414 675 942 1089 1560 1569 1659 1827
Size: 30 261 267 147 471 9 90 168
Cuts at: 1827 2229 2393 2707 3067 4080 4325 384
Size: 402 164 314 360 1013 245 1341
Fragments arranged by size:
1341 1013 471 402 360 314 267 261
245 168 164 147 90 30 9
NaeI GCC'GGC
Cuts at: 693 3463 693
Size: 2770 2512
NarI GG'CG_CC
Cuts at: 924 2713 2961 924
Size: 1789 248 3245
Fragments arranged by size:
3245 1789 248
NcoI C'CATG_G
Cuts at: 659 659
Size: 5282
NgoMIV G'CCGG_C
Cuts at: 691 3461 691
Size: 2770 2512
NsiI A_TGCA'T
Cuts at: 1511 1511
Size: 5282
NspI r_CATG'y
Cuts at: 263 2335 2835 3742 263
Size: 2072 500 907 1803
Fragments arranged by size:
2072 1803 907 500
PasI CC'CwG_GG
Cuts at: 1921 1921
Size: 5282
PciI A'CATG_T
Cuts at: 3738 3738
Size: 5282
PflMI CCAn_nnn'nTGG
Cuts at: 1830 1830
Size: 5282
PfoI T'CCnGG_A
Cuts at: 1607 1694 1838 1985 1607
Size: 87 144 147 4904
Fragments arranged by size:
4904 147 144 87
PmeI GTTT'AAAC
Cuts at: 2281 2281
Size: 5282
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 850 882 4421 4453 850
Size: 32 3539 32 1679
Fragments arranged by size:
3539 1679 32 32
PpuMI rG'GwC_Cy
Cuts at: 1926 1926
Size: 5282
PspOMI G'GGCC_C
Cuts at: 817 1634 817
Size: 817 4465
PspXI vC'TCGA_Gb
Cuts at: 910 910
Size: 5282
PstI C_TGCA'G
Cuts at: 275 334 1082 1139 1451 1964 2106 2329 3014
Size: 59 748 57 312 513 142 223 685
Cuts at: 3014 275
Size: 2543
Fragments arranged by size:
2543 748 685 513 312 223 142 59
57
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5282
PvuII CAG'CTG
Cuts at: 384 414 1089 1560 1569 1659 1827 2707 3067
Size: 30 675 471 9 90 168 880 360
Cuts at: 3067 384
Size: 2599
Fragments arranged by size:
2599 880 675 471 360 168 90 30
9
RsrII CG'GwC_CG
Cuts at: 3477 3477
Size: 5282
SacI G_AGCT'C
Cuts at: 230 1694 2296 230
Size: 1464 602 3216
Fragments arranged by size:
3216 1464 602
SacII CC_GC'GG
Cuts at: 676 676
Size: 5282
SalI G'TCGA_C
Cuts at: 2319 2319
Size: 5282
SapI GCTCTTCn'nnn_
Cuts at: 121 1682 3305 3515 121
Size: 1561 1623 210 1888
Fragments arranged by size:
1888 1623 1561 210
SbfI CC_TGCA'GG
Cuts at: 1451 2329 1451
Size: 878 4404
ScaI AGT'ACT
Cuts at: 760 4799 760
Size: 4039 1243
SfcI C'TryA_G
Cuts at: 33 271 330 1078 1135 1447 1960 2102 2325
Size: 238 59 748 57 312 513 142 223
Cuts at: 2325 3010 4003 4194 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 748 685 513 312 238 223
191 142 59 57
SfoI GGC'GCC
Cuts at: 925 2714 2962 925
Size: 1789 248 3245
Fragments arranged by size:
3245 1789 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5282
SmaI CCC'GGG
Cuts at: 2304 2304
Size: 5282
SmlI C'TyrA_G
Cuts at: 834 910 2437 3352 3844 4106 834
Size: 76 1527 915 492 262 2010
Fragments arranged by size:
2010 1527 915 492 262 76
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5282
SphI G_CATG'C
Cuts at: 2335 2835 2335
Size: 500 4782
StuI AGG'CCT
Cuts at: 1357 2122 1357
Size: 765 4517
StyI C'CwwG_G
Cuts at: 659 1934 2416 2770 659
Size: 1275 482 354 3171
Fragments arranged by size:
3171 1275 482 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 967 2026 2865 2998 3457 3666 967
Size: 1059 839 133 459 209 2583
Fragments arranged by size:
2583 1059 839 459 209 133
TatI w'GTAC_w
Cuts at: 758 4797 758
Size: 4039 1243
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 123 155 295 327 835 867 4799 4831 123
Size: 32 140 32 508 32 3932 32 574
Fragments arranged by size:
3932 574 508 140 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3079 3079
Size: 5282
XbaI T'CTAG_A
Cuts at: 363 2313 363
Size: 1950 3332
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2777 2777
Size: 5282
XhoI C'TCGA_G
Cuts at: 910 910
Size: 5282
XmaI C'CCGG_G
Cuts at: 2302 2302
Size: 5282
XmnI GAAnn'nnTTC
Cuts at: 862 862
Size: 5282
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AleI AloI AscI AvrII BaeI BbsI
BglI BmtI BsiWI BsmI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI PacI PmlI
PshAI PsiI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI ZraI