Restriction map for
pF1KB3304
(Circular) (Six-base) MAPSORT of: pF1KB3304.seq Check: 2836 from: 1 to: 4256
pF1KB3304 4256 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 419 1294 419
Size: 875 3381
Acc65I G'GTAC_C
Cuts at: 1272 1272
Size: 4256
AclI AA'CG_TT
Cuts at: 4096 4096
Size: 4256
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2081 2513 2866 3528 2081
Size: 432 353 662 2809
Fragments arranged by size:
2809 662 432 353
AfeI AGC'GCT
Cuts at: 194 411 194
Size: 217 4039
AflIII A'CryG_T
Cuts at: 1176 1608 3408 3689 1176
Size: 432 1800 281 1743
Fragments arranged by size:
1800 1743 432 281
AlwNI CAG_nnn'CTG
Cuts at: 309 748 1009 1561 2999 309
Size: 439 261 552 1438 1566
Fragments arranged by size:
1566 1438 552 439 261
ApaLI G'TGCA_C
Cuts at: 222 3094 222
Size: 2872 1384
ApoI r'AATT_y
Cuts at: 850 1260 3609 3865 850
Size: 410 2349 256 1241
Fragments arranged by size:
2349 1241 410 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4256
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4256
AvaI C'yCGr_G
Cuts at: 1276 1276
Size: 4256
BamHI G'GATC_C
Cuts at: 1281 1281
Size: 4256
BanI G'GyrC_C
Cuts at: 1272 1686 1934 1969 1272
Size: 414 248 35 3559
Fragments arranged by size:
3559 414 248 35
BanII G_rGCy'C
Cuts at: 167 1270 2300 167
Size: 1103 1030 2123
Fragments arranged by size:
2123 1103 1030
BbeI G_GCGC'C
Cuts at: 1690 1938 1690
Size: 248 4008
BbvCI CC'TCA_GC
Cuts at: 413 413
Size: 4256
BciVI GTATCCnnnnn_n'
Cuts at: 2154 3210 4256 2154
Size: 1056 1046 2154
Fragments arranged by size:
2154 1056 1046
BclI T'GATC_A
Cuts at: 639 1776 639
Size: 1137 3119
BfrBI ATG'CAT
Cuts at: 916 916
Size: 4256
BglII A'GATC_T
Cuts at: 1771 3808 1771
Size: 2037 2219
BlpI GC'TnA_GC
Cuts at: 1368 1368
Size: 4256
Bme1580I G_kGCm'C
Cuts at: 226 1881 1974 3098 226
Size: 1655 93 1124 1384
Fragments arranged by size:
1655 1384 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 367 1468 1549 1884 4028 367
Size: 1101 81 335 2144 595
Fragments arranged by size:
2144 1101 595 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2623 2623
Size: 4256
Bpu10I CC'TnA_GC
Cuts at: 413 413
Size: 4256
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 321 1432 2311 3025 3323 321
Size: 1111 879 714 298 1254
Fragments arranged by size:
1254 1111 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 234 579 3960 234
Size: 345 3381 530
Fragments arranged by size:
3381 530 345
BsaAI yAC'GTr
Cuts at: 1609 1609
Size: 4256
BsaBI GATnn'nnATC
Cuts at: 6 1770 3807 6
Size: 1764 2037 455
Fragments arranged by size:
2037 1764 455
BsaHI Gr'CG_yC
Cuts at: 1687 1935 2637 4133 1687
Size: 248 702 1496 1810
Fragments arranged by size:
1810 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1445 1966 2644 3055 3202 3476 1445
Size: 521 678 411 147 274 2225
Fragments arranged by size:
2225 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 581 611 581
Size: 30 4226
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 151 301 347 350 151
Size: 150 46 3 4057
Fragments arranged by size:
4057 150 46 3
BseYI C'CCAG_C
Cuts at: 1681 2439 3104 3512 1681
Size: 758 665 408 2425
Fragments arranged by size:
2425 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 949 979 235
Size: 714 30 3512
Fragments arranged by size:
3512 714 30
BsiEI CG_ry'CG
Cuts at: 104 789 1798 1844 3074 104
Size: 685 1009 46 1230 1286
Fragments arranged by size:
1286 1230 1009 685 46
BsiHKAI G_wGCw'C
Cuts at: 167 226 1270 2048 2238 3098 167
Size: 59 1044 778 190 860 1325
Fragments arranged by size:
1325 1044 860 778 190 59
BsmI GAATG_Cn'
Cuts at: 250 250
Size: 4256
BsmBI CGTCTCn'nnnn_
Cuts at: 643 643
Size: 4256
Bsp1286I G_dGCh'C
Cuts at: 167 226 1270 1881 1974 2048 2238 2300 3098
Size: 59 1044 611 93 74 190 62 798
Cuts at: 3098 167
Size: 1325
Fragments arranged by size:
1325 1044 798 611 190 93 74 62
59
BspHI T'CATG_A
Cuts at: 2688 2688
Size: 4256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1306 1520 1822 2203 1306
Size: 214 302 381 3359
Fragments arranged by size:
3359 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2548 2602 4086 4251 50
Size: 2498 54 1484 165 55
Fragments arranged by size:
2498 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2168 2168
Size: 4256
BsrFI r'CCGG_y
Cuts at: 2254 2435 2254
Size: 181 4075
BssSI C'ACGA_G
Cuts at: 1109 1130 2527 3235 1109
Size: 21 1397 708 2130
Fragments arranged by size:
2130 1397 708 21
BstAPI GCAn_nnn'nTGC
Cuts at: 1633 1633
Size: 4256
BstBI TT'CG_AA
Cuts at: 2617 2617
Size: 4256
BstXI CCAn_nnnn'nTGG
Cuts at: 375 375
Size: 4256
BstYI r'GATC_y
Cuts at: 1 1281 1748 1771 2106 2352 2658 2670 2756
Size: 1280 467 23 335 246 306 12 86
Cuts at: 2756 3808 1
Size: 1052 449
Fragments arranged by size:
1280 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 256 256
Size: 4256
BtgI C'CryG_G
Cuts at: 107 245 107
Size: 138 4118
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2206 2387 2550 2206
Size: 181 163 3912
Fragments arranged by size:
3912 181 163
BtsI GCAGTG_nn'
Cuts at: 224 1499 224
Size: 1275 2981
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1107 1142 1107
Size: 35 4221
DraI TTT'AAA
Cuts at: 1255 1255
Size: 4256
DrdI GACnn_nn'nnGTC
Cuts at: 1962 3306 1962
Size: 1344 2912
EaeI y'GGCC_r
Cuts at: 482 1841 2015 2406 2433 3778 482
Size: 1359 174 391 27 1345 960
Fragments arranged by size:
1359 1345 960 391 174 27
EagI C'GGCC_G
Cuts at: 1841 1841
Size: 4256
EarI CTCTTCn'nnn_
Cuts at: 1031 2279 2489 1031
Size: 1248 210 2798
Fragments arranged by size:
2798 1248 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2464 3206 3352 4066 2464
Size: 742 146 714 2654
Fragments arranged by size:
2654 742 714 146
EcoICRI GAG'CTC
Cuts at: 165 1268 165
Size: 1103 3153
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2081 2513 2866 3528 2081
Size: 432 353 662 2809
Fragments arranged by size:
2809 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1063 1395 1063
Size: 332 3924
EcoRI G'AATT_C
Cuts at: 850 1260 850
Size: 410 3846
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2275 2275
Size: 4256
FspI TGC'GCA
Cuts at: 2037 2037
Size: 4256
HaeII r_GCGC'y
Cuts at: 196 413 1690 1938 3168 3939 196
Size: 217 1277 248 1230 771 513
Fragments arranged by size:
1277 1230 771 513 248 217
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 291 323 342 374 389 421 424 456 1089
Size: 32 19 32 15 32 3 32 633
Cuts at: 1089 1121 2098 2128 2130 2160 291
Size: 32 977 30 2 30 2387
Fragments arranged by size:
2387 977 633 32 32 32 32 32
30 30 19 15 3 2
HincII GTy'rAC
Cuts at: 666 1295 666
Size: 629 3627
HpaI GTT'AAC
Cuts at: 666 666
Size: 4256
KasI G'GCGC_C
Cuts at: 1686 1934 1686
Size: 248 4008
KpnI G_GTAC'C
Cuts at: 1276 1276
Size: 4256
MluI A'CGCG_T
Cuts at: 3689 3689
Size: 4256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 257 3015 3199 257
Size: 2758 184 1314
Fragments arranged by size:
2758 1314 184
MscI TGG'CCA
Cuts at: 2017 2017
Size: 4256
MslI CAynn'nnrTG
Cuts at: 530 2245 2372 530
Size: 1715 127 2414
Fragments arranged by size:
2414 1715 127
MspA1I CmG'CkG
Cuts at: 1006 1367 1681 2041 2825 3070 1006
Size: 361 314 360 784 245 2192
Fragments arranged by size:
2192 784 361 360 314 245
NaeI GCC'GGC
Cuts at: 2437 2437
Size: 4256
NarI GG'CG_CC
Cuts at: 1687 1935 1687
Size: 248 4008
NcoI C'CATG_G
Cuts at: 107 245 107
Size: 138 4118
NgoMIV G'CCGG_C
Cuts at: 2435 2435
Size: 4256
NsiI A_TGCA'T
Cuts at: 918 918
Size: 4256
NspI r_CATG'y
Cuts at: 1309 1809 3412 1309
Size: 500 1603 2153
Fragments arranged by size:
2153 1603 500
PciI A'CATG_T
Cuts at: 3408 3408
Size: 4256
PmeI GTTT'AAAC
Cuts at: 1255 1255
Size: 4256
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2702 2734 2702
Size: 32 4224
PshAI GACnn'nnGTC
Cuts at: 655 655
Size: 4256
PstI C_TGCA'G
Cuts at: 314 1303 1988 314
Size: 989 685 2582
Fragments arranged by size:
2582 989 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4256
PvuII CAG'CTG
Cuts at: 1006 1681 2041 1006
Size: 675 360 3221
Fragments arranged by size:
3221 675 360
RsrII CG'GwC_CG
Cuts at: 2451 2451
Size: 4256
SacI G_AGCT'C
Cuts at: 167 1270 167
Size: 1103 3153
SalI G'TCGA_C
Cuts at: 1293 1293
Size: 4256
SapI GCTCTTCn'nnn_
Cuts at: 2279 2489 2279
Size: 210 4046
SbfI CC_TGCA'GG
Cuts at: 1303 1303
Size: 4256
ScaI AGT'ACT
Cuts at: 3773 3773
Size: 4256
SfcI C'TryA_G
Cuts at: 33 310 798 1299 1984 2952 3143 33
Size: 277 488 501 685 968 191 1146
Fragments arranged by size:
1146 968 685 501 488 277 191
SfoI GGC'GCC
Cuts at: 1688 1936 1688
Size: 248 4008
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4256
SmaI CCC'GGG
Cuts at: 1278 1278
Size: 4256
SmlI C'TyrA_G
Cuts at: 336 1411 2326 3040 3302 336
Size: 1075 915 714 262 1290
Fragments arranged by size:
1290 1075 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4256
SphI G_CATG'C
Cuts at: 1309 1809 1309
Size: 500 3756
StyI C'CwwG_G
Cuts at: 107 245 1390 1744 107
Size: 138 1145 354 2619
Fragments arranged by size:
2619 1145 354 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 381 561 803 1013 1175 1839 1972 2431 2640
Size: 180 242 210 162 664 133 459 209
Cuts at: 2640 381
Size: 1997
Fragments arranged by size:
1997 664 459 242 210 209 180 162
133
TatI w'GTAC_w
Cuts at: 1223 3771 1223
Size: 2548 1708
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 581 613 3773 3805 581
Size: 32 3160 32 1032
Fragments arranged by size:
3160 1032 32 32
Tth111I GACn'n_nGTC
Cuts at: 2053 2053
Size: 4256
XbaI T'CTAG_A
Cuts at: 1287 1287
Size: 4256
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1751 1751
Size: 4256
XmaI C'CCGG_G
Cuts at: 1276 1276
Size: 4256
XmnI GAAnn'nnTTC
Cuts at: 1178 1178
Size: 4256
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PmeI PpiI
PshAI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BglI BmgBI
BmtI BpmI BsiWI BspEI BsrGI BssHII BstEII BstZ17I
ClaI DraIII EcoNI EcoRV FseI FspAI HindIII MfeI
NdeI NheI NotI NruI PacI PasI PflMI PfoI
PmlI PpuMI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI