Restriction map for
pF1KB3305
(Circular) (Six-base) MAPSORT of: pF1KB3305.seq Check: 622 from: 1 to: 4844
pF1KB3305 4844 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 808 1323 808
Size: 515 4329
AccI GT'mk_AC
Cuts at: 1882 1882
Size: 4844
Acc65I G'GTAC_C
Cuts at: 1860 1860
Size: 4844
AclI AA'CG_TT
Cuts at: 4684 4684
Size: 4844
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 964 1024 2669 3101 3454 4116 574
Size: 390 60 1645 432 353 662 1302
Fragments arranged by size:
1645 1302 662 432 390 353 60
AflIII A'CryG_T
Cuts at: 1584 2196 3996 4277 1584
Size: 612 1800 281 2151
Fragments arranged by size:
2151 1800 612 281
AleI CACnn'nnGTG
Cuts at: 1583 1583
Size: 4844
AlwNI CAG_nnn'CTG
Cuts at: 1603 2149 3587 1603
Size: 546 1438 2860
Fragments arranged by size:
2860 1438 546
ApaI G_GGCC'C
Cuts at: 669 669
Size: 4844
ApaLI G'TGCA_C
Cuts at: 844 3682 844
Size: 2838 2006
ApoI r'AATT_y
Cuts at: 353 1650 1848 4197 4453 353
Size: 1297 198 2349 256 744
Fragments arranged by size:
2349 1297 744 256 198
AseI AT'TA_AT
Cuts at: 20 20
Size: 4844
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4844
AvaI C'yCGr_G
Cuts at: 626 1315 1864 626
Size: 689 549 3606
Fragments arranged by size:
3606 689 549
BamHI G'GATC_C
Cuts at: 1869 1869
Size: 4844
BanI G'GyrC_C
Cuts at: 327 605 711 730 1360 1860 2274 2522 2557
Size: 278 106 19 630 500 414 248 35
Cuts at: 2557 327
Size: 2614
Fragments arranged by size:
2614 630 500 414 278 248 106 35
19
BanII G_rGCy'C
Cuts at: 649 669 1858 2888 649
Size: 20 1189 1030 2605
Fragments arranged by size:
2605 1189 1030 20
BbeI G_GCGC'C
Cuts at: 609 1364 2278 2526 609
Size: 755 914 248 2927
Fragments arranged by size:
2927 914 755 248
BbvCI CC'TCA_GC
Cuts at: 1664 1664
Size: 4844
BciVI GTATCCnnnnn_n'
Cuts at: 2742 3798 4844 2742
Size: 1056 1046 2742
Fragments arranged by size:
2742 1056 1046
BclI T'GATC_A
Cuts at: 2364 2364
Size: 4844
BfrBI ATG'CAT
Cuts at: 900 900
Size: 4844
BglI GCCn_nnn'nGGC
Cuts at: 614 614
Size: 4844
BglII A'GATC_T
Cuts at: 185 2359 4396 185
Size: 2174 2037 633
Fragments arranged by size:
2174 2037 633
BlpI GC'TnA_GC
Cuts at: 1956 1956
Size: 4844
Bme1580I G_kGCm'C
Cuts at: 332 669 716 848 2469 2562 3686 332
Size: 337 47 132 1621 93 1124 1490
Fragments arranged by size:
1621 1490 1124 337 132 93 47
BmgBI CAC'GTC
Cuts at: 798 798
Size: 4844
BmrI ACTGGGnnnn_n'
Cuts at: 261 714 777 1289 2056 2137 2472 4616 261
Size: 453 63 512 767 81 335 2144 489
Fragments arranged by size:
2144 767 512 489 453 335 81 63
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 882 3211 882
Size: 2329 2515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1591 1591
Size: 4844
Bpu10I CC'TnA_GC
Cuts at: 911 1505 1664 911
Size: 594 159 4091
Fragments arranged by size:
4091 594 159
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1513 2020 2899 3613 3911 1513
Size: 507 879 714 298 2446
Fragments arranged by size:
2446 879 714 507 298
BsaI GGTCTCn'nnnn_
Cuts at: 564 1567 1676 4548 564
Size: 1003 109 2872 860
Fragments arranged by size:
2872 1003 860 109
BsaAI yAC'GTr
Cuts at: 1585 2197 1585
Size: 612 4232
BsaBI GATnn'nnATC
Cuts at: 6 2358 4395 6
Size: 2352 2037 455
Fragments arranged by size:
2352 2037 455
BsaHI Gr'CG_yC
Cuts at: 606 975 1361 2275 2523 3225 4721 606
Size: 369 386 914 248 702 1496 729
Fragments arranged by size:
1496 914 729 702 386 369 248
BsaWI w'CCGG_w
Cuts at: 2033 2554 3232 3643 3790 4064 2033
Size: 521 678 411 147 274 2813
Fragments arranged by size:
2813 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 154 172 184 202 365 395 154
Size: 18 12 18 163 30 4603
Fragments arranged by size:
4603 163 30 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 858 1035 1549 858
Size: 177 514 4153
Fragments arranged by size:
4153 514 177
BseYI C'CCAG_C
Cuts at: 377 669 1236 1267 2269 3027 3692 4100 377
Size: 292 567 31 1002 758 665 408 1121
Fragments arranged by size:
1121 1002 758 665 567 408 292 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 842 1351 842
Size: 509 4335
BsiEI CG_ry'CG
Cuts at: 104 2386 2432 3662 104
Size: 2282 46 1230 1286
Fragments arranged by size:
2282 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 182 251 848 1858 2636 2826 3686 182
Size: 69 597 1010 778 190 860 1340
Fragments arranged by size:
1340 1010 860 778 597 190 69
BsmBI CGTCTCn'nnnn_
Cuts at: 454 982 454
Size: 528 4316
Bsp1286I G_dGCh'C
Cuts at: 182 251 332 649 669 716 848 1858 2469
Size: 69 81 317 20 47 132 1010 611
Cuts at: 2469 2562 2636 2826 2888 3686 182
Size: 93 74 190 62 798 1340
Fragments arranged by size:
1340 1010 798 611 317 190 132 93
81 74 69 62 47 20
BspHI T'CATG_A
Cuts at: 515 3276 515
Size: 2761 2083
BspMI ACCTGCnnnn'nnnn_
Cuts at: 808 1323 1894 2108 2410 2791 808
Size: 515 571 214 302 381 2861
Fragments arranged by size:
2861 571 515 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3136 3190 4674 4839 50
Size: 3086 54 1484 165 55
Fragments arranged by size:
3086 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2756 2756
Size: 4844
BsrFI r'CCGG_y
Cuts at: 2842 3023 2842
Size: 181 4663
BssSI C'ACGA_G
Cuts at: 3115 3823 3115
Size: 708 4136
BstAPI GCAn_nnn'nTGC
Cuts at: 2221 2221
Size: 4844
BstBI TT'CG_AA
Cuts at: 3205 3205
Size: 4844
BstXI CCAn_nnnn'nTGG
Cuts at: 1749 1749
Size: 4844
BstYI r'GATC_y
Cuts at: 1 185 947 1763 1869 2336 2359 2694 2940
Size: 184 762 816 106 467 23 335 246
Cuts at: 2940 3246 3258 3344 4396 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 816 762 467 449 335 306 246
184 106 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 857 857
Size: 4844
BtgI C'CryG_G
Cuts at: 152 152
Size: 4844
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 271 2794 2975 3138 271
Size: 2523 181 163 1977
Fragments arranged by size:
2523 1977 181 163
BtsI GCAGTG_nn'
Cuts at: 473 1361 2087 473
Size: 888 726 3230
Fragments arranged by size:
3230 888 726
DraI TTT'AAA
Cuts at: 1843 1843
Size: 4844
DrdI GACnn_nn'nnGTC
Cuts at: 2550 3894 2550
Size: 1344 3500
EaeI y'GGCC_r
Cuts at: 198 528 1605 2429 2603 2994 3021 4366 198
Size: 330 1077 824 174 391 27 1345 676
Fragments arranged by size:
1345 1077 824 676 391 330 174 27
EagI C'GGCC_G
Cuts at: 2429 2429
Size: 4844
EarI CTCTTCn'nnn_
Cuts at: 2867 3077 2867
Size: 210 4634
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3052 3794 3940 4654 3052
Size: 742 146 714 3242
Fragments arranged by size:
3242 742 714 146
EcoICRI GAG'CTC
Cuts at: 1856 1856
Size: 4844
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 964 1024 1591 2669 3101 3454 4116 574
Size: 390 60 567 1078 432 353 662 1302
Fragments arranged by size:
1302 1078 662 567 432 390 353 60
EcoNI CCTnn'n_nnAGG
Cuts at: 1006 1780 1006
Size: 774 4070
EcoO109I rG'GnC_Cy
Cuts at: 592 665 666 1983 592
Size: 73 1 1317 3453
Fragments arranged by size:
3453 1317 73 1
EcoRI G'AATT_C
Cuts at: 1848 1848
Size: 4844
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 903 2863 903
Size: 1960 2884
FspI TGC'GCA
Cuts at: 2625 2625
Size: 4844
HaeII r_GCGC'y
Cuts at: 609 1364 1418 2278 2526 3756 4527 609
Size: 755 54 860 248 1230 771 926
Fragments arranged by size:
1230 926 860 771 755 248 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2686 2716 2718 2748 2686
Size: 30 2 30 4782
Fragments arranged by size:
4782 30 30 2
HincII GTy'rAC
Cuts at: 1883 1883
Size: 4844
HindIII A'AGCT_T
Cuts at: 115 115
Size: 4844
KasI G'GCGC_C
Cuts at: 605 1360 2274 2522 605
Size: 755 914 248 2927
Fragments arranged by size:
2927 914 755 248
KpnI G_GTAC'C
Cuts at: 1864 1864
Size: 4844
MluI A'CGCG_T
Cuts at: 4277 4277
Size: 4844
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 512 518 3603 3787 512
Size: 6 3085 184 1569
Fragments arranged by size:
3085 1569 184 6
MscI TGG'CCA
Cuts at: 530 2605 530
Size: 2075 2769
MslI CAynn'nnrTG
Cuts at: 841 1583 1585 1736 2833 2960 841
Size: 742 2 151 1097 127 2725
Fragments arranged by size:
2725 1097 742 151 127 2
MspA1I CmG'CkG
Cuts at: 154 197 661 826 1603 1663 1955 2269 2629
Size: 43 464 165 777 60 292 314 360
Cuts at: 2629 3413 3658 154
Size: 784 245 1340
Fragments arranged by size:
1340 784 777 464 360 314 292 245
165 60 43
NaeI GCC'GGC
Cuts at: 3025 3025
Size: 4844
NarI GG'CG_CC
Cuts at: 606 1361 2275 2523 606
Size: 755 914 248 2927
Fragments arranged by size:
2927 914 755 248
NgoMIV G'CCGG_C
Cuts at: 3023 3023
Size: 4844
NsiI A_TGCA'T
Cuts at: 902 902
Size: 4844
NspI r_CATG'y
Cuts at: 1897 2397 4000 1897
Size: 500 1603 2741
Fragments arranged by size:
2741 1603 500
PciI A'CATG_T
Cuts at: 3996 3996
Size: 4844
PflMI CCAn_nnn'nTGG
Cuts at: 207 537 726 1356 207
Size: 330 189 630 3695
Fragments arranged by size:
3695 630 330 189
PfoI T'CCnGG_A
Cuts at: 280 280
Size: 4844
PmeI GTTT'AAAC
Cuts at: 1843 1843
Size: 4844
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3290 3322 3290
Size: 32 4812
PpuMI rG'GwC_Cy
Cuts at: 592 592
Size: 4844
PspOMI G'GGCC_C
Cuts at: 665 665
Size: 4844
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 103 135 103
Size: 32 4812
PstI C_TGCA'G
Cuts at: 1190 1891 2576 1190
Size: 701 685 3458
Fragments arranged by size:
3458 701 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4844
PvuII CAG'CTG
Cuts at: 826 1603 1663 2269 2629 826
Size: 777 60 606 360 3041
Fragments arranged by size:
3041 777 606 360 60
RsrII CG'GwC_CG
Cuts at: 3039 3039
Size: 4844
SacI G_AGCT'C
Cuts at: 1858 1858
Size: 4844
SacII CC_GC'GG
Cuts at: 155 155
Size: 4844
SalI G'TCGA_C
Cuts at: 1881 1881
Size: 4844
SapI GCTCTTCn'nnn_
Cuts at: 2867 3077 2867
Size: 210 4634
SbfI CC_TGCA'GG
Cuts at: 1891 1891
Size: 4844
ScaI AGT'ACT
Cuts at: 395 4361 395
Size: 3966 878
SexAI A'CCwGG_T
Cuts at: 1706 1706
Size: 4844
SfcI C'TryA_G
Cuts at: 33 192 1186 1223 1293 1887 2572 3540 3731
Size: 159 994 37 70 594 685 968 191
Cuts at: 3731 33
Size: 1146
Fragments arranged by size:
1146 994 968 685 594 191 159 70
37
SfoI GGC'GCC
Cuts at: 607 1362 2276 2524 607
Size: 755 914 248 2927
Fragments arranged by size:
2927 914 755 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4844
SmaI CCC'GGG
Cuts at: 1866 1866
Size: 4844
SmlI C'TyrA_G
Cuts at: 1528 1999 2914 3628 3890 1528
Size: 471 915 714 262 2482
Fragments arranged by size:
2482 915 714 471 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4844
SphI G_CATG'C
Cuts at: 1897 2397 1897
Size: 500 4344
SspI AAT'ATT
Cuts at: 369 369
Size: 4844
StuI AGG'CCT
Cuts at: 1156 1156
Size: 4844
StyI C'CwwG_G
Cuts at: 1553 1978 2332 1553
Size: 425 354 4065
Fragments arranged by size:
4065 425 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 718 733 2427 2560 3019 3228 718
Size: 15 1694 133 459 209 2334
Fragments arranged by size:
2334 1694 459 209 133 15
TatI w'GTAC_w
Cuts at: 393 4359 393
Size: 3966 878
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 172 204 365 397 1619 1651 4361 4393 172
Size: 32 161 32 1222 32 2710 32 623
Fragments arranged by size:
2710 1222 623 161 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 783 1708 2641 783
Size: 925 933 2986
Fragments arranged by size:
2986 933 925
XbaI T'CTAG_A
Cuts at: 1875 1875
Size: 4844
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 723 1461 2339 723
Size: 738 878 3228
Fragments arranged by size:
3228 878 738
XmaI C'CCGG_G
Cuts at: 1864 1864
Size: 4844
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI AscI
AvrII BaeI BbsI BcgI BmtI BsiWI BsmI BspEI
BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HpaI MfeI NcoI NdeI NheI NotI
NruI PacI PasI PmlI PshAI PsiI PspXI SanDI
SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI