Restriction map for
pF1KB3307
(Circular) (Six-base) MAPSORT of: pF1KB3307.seq Check: 1177 from: 1 to: 5267
pF1KB3307 5267 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 184 2305 184
Size: 2121 3146
Acc65I G'GTAC_C
Cuts at: 2283 2283
Size: 5267
AclI AA'CG_TT
Cuts at: 5107 5107
Size: 5267
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 889 890 1684 3092 3524 3877 4539 889
Size: 1 794 1408 432 353 662 1617
Fragments arranged by size:
1617 1408 794 662 432 353 1
AflIII A'CryG_T
Cuts at: 1229 2619 4419 4700 1229
Size: 1390 1800 281 1796
Fragments arranged by size:
1800 1796 1390 281
AleI CACnn'nnGTG
Cuts at: 1764 1764
Size: 5267
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1850 1929 1850
Size: 79 5188
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 874 906 874
Size: 32 5235
AlwNI CAG_nnn'CTG
Cuts at: 388 397 451 535 679 1255 1373 1662 2013
Size: 9 54 84 144 576 118 289 351
Cuts at: 2013 2053 2214 2572 4010 388
Size: 40 161 358 1438 1645
Fragments arranged by size:
1645 1438 576 358 351 289 161 144
118 84 54 40 9
ApaI G_GGCC'C
Cuts at: 835 915 2088 835
Size: 80 1173 4014
Fragments arranged by size:
4014 1173 80
ApaLI G'TGCA_C
Cuts at: 1732 4105 1732
Size: 2373 2894
ApoI r'AATT_y
Cuts at: 1669 2255 2271 4620 4876 1669
Size: 586 16 2349 256 2060
Fragments arranged by size:
2349 2060 586 256 16
AseI AT'TA_AT
Cuts at: 20 20
Size: 5267
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5267
AvaI C'yCGr_G
Cuts at: 2162 2287 2162
Size: 125 5142
AvrII C'CTAG_G
Cuts at: 546 546
Size: 5267
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 377 410 377
Size: 33 5234
BamHI G'GATC_C
Cuts at: 2292 2292
Size: 5267
BanI G'GyrC_C
Cuts at: 1705 1834 2283 2697 2945 2980 1705
Size: 129 449 414 248 35 3992
Fragments arranged by size:
3992 449 414 248 129 35
BanII G_rGCy'C
Cuts at: 634 835 915 1040 1063 1413 2088 2281 3311
Size: 201 80 125 23 350 675 193 1030
Cuts at: 3311 634
Size: 2590
Fragments arranged by size:
2590 1030 675 350 201 193 125 80
23
BbeI G_GCGC'C
Cuts at: 2701 2949 2701
Size: 248 5019
BbsI GAAGACnn'nnnn_
Cuts at: 896 1232 896
Size: 336 4931
BbvCI CC'TCA_GC
Cuts at: 967 2065 967
Size: 1098 4169
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 164 198 1445 1479 164
Size: 34 1247 34 3952
Fragments arranged by size:
3952 1247 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3165 4221 5267 3165
Size: 1056 1046 3165
Fragments arranged by size:
3165 1056 1046
BclI T'GATC_A
Cuts at: 2787 2787
Size: 5267
BglI GCCn_nnn'nGGC
Cuts at: 1935 1999 1935
Size: 64 5203
BglII A'GATC_T
Cuts at: 2782 4819 2782
Size: 2037 3230
BlpI GC'TnA_GC
Cuts at: 2379 2379
Size: 5267
Bme1580I G_kGCm'C
Cuts at: 835 915 1208 1736 1839 2088 2892 2985 4109
Size: 80 293 528 103 249 804 93 1124
Cuts at: 4109 835
Size: 1993
Fragments arranged by size:
1993 1124 804 528 293 249 103 93
80
BmgBI CAC'GTC
Cuts at: 1731 1731
Size: 5267
BmrI ACTGGGnnnn_n'
Cuts at: 1218 2479 2560 2895 5039 1218
Size: 1261 81 335 2144 1446
Fragments arranged by size:
2144 1446 1261 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1888 3634 1888
Size: 1746 3521
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 448 492 1718 1798 1861 326
Size: 122 44 1226 80 63 3732
Fragments arranged by size:
3732 1226 122 80 63 44
Bpu10I CC'TnA_GC
Cuts at: 242 967 2065 242
Size: 725 1098 3444
Fragments arranged by size:
3444 1098 725
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 1303 2443 3322 4036 4334 386
Size: 917 1140 879 714 298 1319
Fragments arranged by size:
1319 1140 917 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4971 4971
Size: 5267
BsaAI yAC'GTr
Cuts at: 2620 2620
Size: 5267
BsaBI GATnn'nnATC
Cuts at: 6 2781 4818 6
Size: 2775 2037 455
Fragments arranged by size:
2775 2037 455
BsaHI Gr'CG_yC
Cuts at: 2698 2946 3648 5144 2698
Size: 248 702 1496 2821
Fragments arranged by size:
2821 1496 702 248
BsaWI w'CCGG_w
Cuts at: 692 2456 2977 3655 4066 4213 4487 692
Size: 1764 521 678 411 147 274 1472
Fragments arranged by size:
1764 1472 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 241 781 241
Size: 540 4727
BseYI C'CCAG_C
Cuts at: 1267 2087 2692 3450 4115 4523 1267
Size: 820 605 758 665 408 2011
Fragments arranged by size:
2011 820 758 665 605 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1261 1322 2227 1261
Size: 61 905 4301
Fragments arranged by size:
4301 905 61
BsiEI CG_ry'CG
Cuts at: 104 2809 2855 4085 104
Size: 2705 46 1230 1286
Fragments arranged by size:
2705 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1040 1736 1874 2281 3059 3249 4109 1040
Size: 696 138 407 778 190 860 2198
Fragments arranged by size:
2198 860 778 696 407 190 138
Bsp1286I G_dGCh'C
Cuts at: 634 835 915 1040 1063 1208 1413 1736 1839
Size: 201 80 125 23 145 205 323 103
Cuts at: 1839 1874 2088 2281 2892 2985 3059 3249 3311
Size: 35 214 193 611 93 74 190 62
Cuts at: 3311 4109 634
Size: 798 1792
Fragments arranged by size:
1792 798 611 323 214 205 201 193
190 145 125 103 93 80 74 62
35 23
BspEI T'CCGG_A
Cuts at: 692 692
Size: 5267
BspHI T'CATG_A
Cuts at: 3699 3699
Size: 5267
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2317 2531 2833 3214 2317
Size: 214 302 381 4370
Fragments arranged by size:
4370 381 302 214
BsrBI CCG'CTC
Cuts at: 50 210 432 654 3559 3613 5097 5262 50
Size: 160 222 222 2905 54 1484 165 55
Fragments arranged by size:
2905 1484 222 222 165 160 55 54
BsrDI GCAATG_nn'
Cuts at: 3179 3179
Size: 5267
BsrFI r'CCGG_y
Cuts at: 1013 1361 1754 3265 3446 1013
Size: 348 393 1511 181 2834
Fragments arranged by size:
2834 1511 393 348 181
BsrGI T'GTAC_A
Cuts at: 1495 1495
Size: 5267
BssSI C'ACGA_G
Cuts at: 1085 2232 3538 4246 1085
Size: 1147 1306 708 2106
Fragments arranged by size:
2106 1306 1147 708
BstAPI GCAn_nnn'nTGC
Cuts at: 388 397 2013 2644 388
Size: 9 1616 631 3011
Fragments arranged by size:
3011 1616 631 9
BstBI TT'CG_AA
Cuts at: 3628 3628
Size: 5267
BstYI r'GATC_y
Cuts at: 1 1369 1520 2292 2759 2782 3117 3363 3669
Size: 1368 151 772 467 23 335 246 306
Cuts at: 3669 3681 3767 4819 1
Size: 12 86 1052 449
Fragments arranged by size:
1368 1052 772 467 449 335 306 246
151 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2198 2198
Size: 5267
BtgI C'CryG_G
Cuts at: 107 107
Size: 5267
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3217 3398 3561 3217
Size: 181 163 4923
Fragments arranged by size:
4923 181 163
BtsI GCAGTG_nn'
Cuts at: 398 500 922 1341 1874 2510 398
Size: 102 422 419 533 636 3155
Fragments arranged by size:
3155 636 533 422 419 102
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 182 217 182
Size: 35 5232
DraI TTT'AAA
Cuts at: 2266 2266
Size: 5267
DrdI GACnn_nn'nnGTC
Cuts at: 2973 4317 2973
Size: 1344 3923
EaeI y'GGCC_r
Cuts at: 1597 1756 1927 2121 2852 3026 3417 3444 4789
Size: 159 171 194 731 174 391 27 1345
Cuts at: 4789 1597
Size: 2075
Fragments arranged by size:
2075 1345 731 391 194 174 171 159
27
EagI C'GGCC_G
Cuts at: 2852 2852
Size: 5267
EarI CTCTTCn'nnn_
Cuts at: 1045 3290 3500 1045
Size: 2245 210 2812
Fragments arranged by size:
2812 2245 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1567 2068 3475 4217 4363 5077 1567
Size: 501 1407 742 146 714 1757
Fragments arranged by size:
1757 1407 742 714 501 146
EcoICRI GAG'CTC
Cuts at: 1038 2279 1038
Size: 1241 4026
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 448 492 889 890 1684 1718 1798 1861
Size: 122 44 397 1 794 34 80 63
Cuts at: 1861 3092 3524 3877 4539 326
Size: 1231 432 353 662 1054
Fragments arranged by size:
1231 1054 794 662 432 397 353 122
80 63 44 34 1
EcoNI CCTnn'n_nnAGG
Cuts at: 223 610 693 826 897 1654 223
Size: 387 83 133 71 757 3836
Fragments arranged by size:
3836 757 387 133 83 71
EcoO109I rG'GnC_Cy
Cuts at: 831 832 911 912 1617 1647 1659 1686 2238
Size: 1 79 1 705 30 12 27 552
Cuts at: 2238 2406 831
Size: 168 3692
Fragments arranged by size:
3692 705 552 168 79 30 27 12
1 1
EcoRI G'AATT_C
Cuts at: 2271 2271
Size: 5267
EcoRV GAT'ATC
Cuts at: 296 296
Size: 5267
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 774 3286 774
Size: 2512 2755
FseI GG_CCGG'CC
Cuts at: 1758 1758
Size: 5267
FspI TGC'GCA
Cuts at: 3048 3048
Size: 5267
HaeII r_GCGC'y
Cuts at: 2701 2949 4179 4950 2701
Size: 248 1230 771 3018
Fragments arranged by size:
3018 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1678 1710 1796 1828 2035 2048 2067 2080 3109
Size: 32 86 32 207 13 19 13 1029
Cuts at: 3109 3139 3141 3171 1678
Size: 30 2 30 3774
Fragments arranged by size:
3774 1029 207 86 32 32 30 30
19 13 13 2
HincII GTy'rAC
Cuts at: 185 819 2306 185
Size: 634 1487 3146
Fragments arranged by size:
3146 1487 634
HindIII A'AGCT_T
Cuts at: 751 751
Size: 5267
KasI G'GCGC_C
Cuts at: 2697 2945 2697
Size: 248 5019
KpnI G_GTAC'C
Cuts at: 2287 2287
Size: 5267
MluI A'CGCG_T
Cuts at: 4700 4700
Size: 5267
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 808 1815 4026 4210 808
Size: 1007 2211 184 1865
Fragments arranged by size:
2211 1865 1007 184
MscI TGG'CCA
Cuts at: 1929 2123 3028 1929
Size: 194 905 4168
Fragments arranged by size:
4168 905 194
MslI CAynn'nnrTG
Cuts at: 894 1764 1766 1966 3256 3383 894
Size: 870 2 200 1290 127 2778
Fragments arranged by size:
2778 1290 870 200 127 2
MspA1I CmG'CkG
Cuts at: 535 676 1509 2378 2692 3052 3836 4081 535
Size: 141 833 869 314 360 784 245 1721
Fragments arranged by size:
1721 869 833 784 360 314 245 141
NaeI GCC'GGC
Cuts at: 1756 3448 1756
Size: 1692 3575
NarI GG'CG_CC
Cuts at: 2698 2946 2698
Size: 248 5019
NcoI C'CATG_G
Cuts at: 107 107
Size: 5267
NgoMIV G'CCGG_C
Cuts at: 1754 3446 1754
Size: 1692 3575
NspI r_CATG'y
Cuts at: 1233 2320 2820 4423 1233
Size: 1087 500 1603 2077
Fragments arranged by size:
2077 1603 1087 500
PasI CC'CwG_GG
Cuts at: 1063 1682 1749 1063
Size: 619 67 4581
Fragments arranged by size:
4581 619 67
PciI A'CATG_T
Cuts at: 1229 4419 1229
Size: 3190 2077
PflMI CCAn_nnn'nTGG
Cuts at: 522 1373 1956 522
Size: 851 583 3833
Fragments arranged by size:
3833 851 583
PfoI T'CCnGG_A
Cuts at: 303 303
Size: 5267
PmeI GTTT'AAAC
Cuts at: 2266 2266
Size: 5267
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1022 1054 3713 3745 1022
Size: 32 2659 32 2544
Fragments arranged by size:
2659 2544 32 32
PpuMI rG'GwC_Cy
Cuts at: 1617 1647 1686 2238 1617
Size: 30 39 552 4646
Fragments arranged by size:
4646 552 39 30
PspOMI G'GGCC_C
Cuts at: 831 911 2084 831
Size: 80 1173 4014
Fragments arranged by size:
4014 1173 80
PstI C_TGCA'G
Cuts at: 393 565 929 2025 2314 2999 393
Size: 172 364 1096 289 685 2661
Fragments arranged by size:
2661 1096 685 364 289 172
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5267
PvuII CAG'CTG
Cuts at: 535 676 1509 2692 3052 535
Size: 141 833 1183 360 2750
Fragments arranged by size:
2750 1183 833 360 141
RsrII CG'GwC_CG
Cuts at: 3462 3462
Size: 5267
SacI G_AGCT'C
Cuts at: 1040 2281 1040
Size: 1241 4026
SalI G'TCGA_C
Cuts at: 183 2304 183
Size: 2121 3146
SanDI GG'GwC_CC
Cuts at: 1617 2238 1617
Size: 621 4646
SapI GCTCTTCn'nnn_
Cuts at: 1045 3290 3500 1045
Size: 2245 210 2812
Fragments arranged by size:
2812 2245 210
SbfI CC_TGCA'GG
Cuts at: 2314 2314
Size: 5267
ScaI AGT'ACT
Cuts at: 4784 4784
Size: 5267
SfcI C'TryA_G
Cuts at: 33 389 561 925 2021 2224 2310 2995 3963
Size: 356 172 364 1096 203 86 685 968
Cuts at: 3963 4154 33
Size: 191 1146
Fragments arranged by size:
1146 1096 968 685 364 356 203 191
172 86
SfoI GGC'GCC
Cuts at: 2699 2947 2699
Size: 248 5019
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5267
SgrAI Cr'CCGG_yG
Cuts at: 1013 1013
Size: 5267
SmaI CCC'GGG
Cuts at: 2289 2289
Size: 5267
SmlI C'TyrA_G
Cuts at: 401 1318 2162 2422 3337 4051 4313 401
Size: 917 844 260 915 714 262 1355
Fragments arranged by size:
1355 917 915 844 714 262 260
SpeI A'CTAG_T
Cuts at: 67 1110 67
Size: 1043 4224
SphI G_CATG'C
Cuts at: 2320 2820 2320
Size: 500 4767
StuI AGG'CCT
Cuts at: 1263 1263
Size: 5267
StyI C'CwwG_G
Cuts at: 107 144 437 546 2178 2401 2755 107
Size: 37 293 109 1632 223 354 2619
Fragments arranged by size:
2619 1632 354 293 223 109 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 529 791 2850 2983 3442 3651 529
Size: 262 2059 133 459 209 2145
Fragments arranged by size:
2145 2059 459 262 209 133
TatI w'GTAC_w
Cuts at: 574 1495 4782 574
Size: 921 3287 1059
Fragments arranged by size:
3287 1059 921
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4784 4816 4784
Size: 32 5235
Tth111I GACn'n_nGTC
Cuts at: 3064 3064
Size: 5267
XbaI T'CTAG_A
Cuts at: 2298 2298
Size: 5267
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1018 2762 1018
Size: 1744 3523
XhoI C'TCGA_G
Cuts at: 2162 2162
Size: 5267
XmaI C'CCGG_G
Cuts at: 2287 2287
Size: 5267
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AscI BfrBI
BmtI BsaXI BsiWI BsmI BsmBI BssHII BstEII BstXI
BstZ17I ClaI DraIII FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PmlI PshAI PsiI PspXI
PsrI SacII SexAI SfiI SnaBI SrfI SspI SwaI
XmnI ZraI