Restriction map for
pF1KB3314
(Circular) (Six-base) MAPSORT of: pF1KB3314.seq Check: 4516 from: 1 to: 5240
pF1KB3314 5240 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AatII G_ACGT'C
Cuts at: 626 1813 626
Size: 1187 4053
AccI GT'mk_AC
Cuts at: 2278 2278
Size: 5240
Acc65I G'GTAC_C
Cuts at: 1680 2256 1680
Size: 576 4664
AclI AA'CG_TT
Cuts at: 5080 5080
Size: 5240
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 1287 1796 2114 3065 3497 3850 4512 260
Size: 1027 509 318 951 432 353 662 988
Fragments arranged by size:
1027 988 951 662 509 432 353 318
AflII C'TTAA_G
Cuts at: 289 289
Size: 5240
AflIII A'CryG_T
Cuts at: 688 2047 2592 4392 4673 688
Size: 1359 545 1800 281 1255
Fragments arranged by size:
1800 1359 1255 545 281
AhdI GACnn_n'nnGTC
Cuts at: 387 612 1362 387
Size: 225 750 4265
Fragments arranged by size:
4265 750 225
AlwNI CAG_nnn'CTG
Cuts at: 397 2545 3983 397
Size: 2148 1438 1654
Fragments arranged by size:
2148 1654 1438
ApaLI G'TGCA_C
Cuts at: 1648 4078 1648
Size: 2430 2810
ApoI r'AATT_y
Cuts at: 136 297 664 1878 2244 4593 4849 136
Size: 161 367 1214 366 2349 256 527
Fragments arranged by size:
2349 1214 527 367 366 256 161
AseI AT'TA_AT
Cuts at: 20 1465 20
Size: 1445 3795
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5240
AvaI C'yCGr_G
Cuts at: 2260 2260
Size: 5240
BamHI G'GATC_C
Cuts at: 2265 2265
Size: 5240
BanI G'GyrC_C
Cuts at: 1680 2256 2670 2918 2953 1680
Size: 576 414 248 35 3967
Fragments arranged by size:
3967 576 414 248 35
BanII G_rGCy'C
Cuts at: 377 2254 3284 377
Size: 1877 1030 2333
Fragments arranged by size:
2333 1877 1030
BbeI G_GCGC'C
Cuts at: 2674 2922 2674
Size: 248 4992
BbsI GAAGACnn'nnnn_
Cuts at: 1171 1171
Size: 5240
BbvCI CC'TCA_GC
Cuts at: 208 208
Size: 5240
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1639 1673 1639
Size: 34 5206
BciVI GTATCCnnnnn_n'
Cuts at: 354 757 3138 4194 5240 354
Size: 403 2381 1056 1046 354
Fragments arranged by size:
2381 1056 1046 403 354
BclI T'GATC_A
Cuts at: 701 2760 701
Size: 2059 3181
BglII A'GATC_T
Cuts at: 285 878 988 2755 4792 285
Size: 593 110 1767 2037 733
Fragments arranged by size:
2037 1767 733 593 110
BlpI GC'TnA_GC
Cuts at: 2352 2352
Size: 5240
Bme1580I G_kGCm'C
Cuts at: 1584 1652 2865 2958 4082 1584
Size: 68 1213 93 1124 2742
Fragments arranged by size:
2742 1213 1124 93 68
BmrI ACTGGGnnnn_n'
Cuts at: 2452 2533 2868 5012 2452
Size: 81 335 2144 2680
Fragments arranged by size:
2680 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 165 167 3607 165
Size: 2 3440 1798
Fragments arranged by size:
3440 1798 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 895 1018 1104 1312 1835 895
Size: 123 86 208 523 4300
Fragments arranged by size:
4300 523 208 123 86
Bpu10I CC'TnA_GC
Cuts at: 208 460 208
Size: 252 4988
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2416 3295 4009 4307 2416
Size: 879 714 298 3349
Fragments arranged by size:
3349 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4944 4944
Size: 5240
BsaAI yAC'GTr
Cuts at: 2048 2593 2048
Size: 545 4695
BsaBI GATnn'nnATC
Cuts at: 6 2153 2754 4791 6
Size: 2147 601 2037 455
Fragments arranged by size:
2147 2037 601 455
BsaHI Gr'CG_yC
Cuts at: 623 1810 2671 2919 3621 5117 623
Size: 1187 861 248 702 1496 746
Fragments arranged by size:
1496 1187 861 746 702 248
BsaWI w'CCGG_w
Cuts at: 168 925 2429 2950 3628 4039 4186 4460 168
Size: 757 1504 521 678 411 147 274 948
Fragments arranged by size:
1504 948 757 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 520 550 520
Size: 30 5210
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 480 826 1309 1337 1705 1753 480
Size: 346 483 28 368 48 3967
Fragments arranged by size:
3967 483 368 346 48 28
BseYI C'CCAG_C
Cuts at: 514 1329 2665 3423 4088 4496 514
Size: 815 1336 758 665 408 1258
Fragments arranged by size:
1336 1258 815 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 529 713 1037 529
Size: 184 324 4732
Fragments arranged by size:
4732 324 184
BsiEI CG_ry'CG
Cuts at: 104 2782 2828 4058 104
Size: 2678 46 1230 1286
Fragments arranged by size:
2678 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 377 1652 2254 3032 3222 4082 377
Size: 1275 602 778 190 860 1535
Fragments arranged by size:
1535 1275 860 778 602 190
Bsp1286I G_dGCh'C
Cuts at: 377 1584 1652 2254 2865 2958 3032 3222 3284
Size: 1207 68 602 611 93 74 190 62
Cuts at: 3284 4082 377
Size: 798 1535
Fragments arranged by size:
1535 1207 798 611 602 190 93 74
68 62
BspHI T'CATG_A
Cuts at: 3672 3672
Size: 5240
BspMI ACCTGCnnnn'nnnn_
Cuts at: 170 2290 2504 2806 3187 170
Size: 2120 214 302 381 2223
Fragments arranged by size:
2223 2120 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3532 3586 5070 5235 50
Size: 3482 54 1484 165 55
Fragments arranged by size:
3482 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1166 3152 1166
Size: 1986 3254
BsrFI r'CCGG_y
Cuts at: 3238 3419 3238
Size: 181 5059
BssSI C'ACGA_G
Cuts at: 1868 3511 4219 1868
Size: 1643 708 2889
Fragments arranged by size:
2889 1643 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2617 2617
Size: 5240
BstBI TT'CG_AA
Cuts at: 1095 3601 1095
Size: 2506 2734
BstYI r'GATC_y
Cuts at: 1 285 716 878 988 2265 2732 2755 3090
Size: 284 431 162 110 1277 467 23 335
Cuts at: 3090 3336 3642 3654 3740 4792 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1277 1052 467 449 431 335 306 284
246 162 110 86 23 12
BtgI C'CryG_G
Cuts at: 107 201 107
Size: 94 5146
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1255 3190 3371 3534 1255
Size: 1935 181 163 2961
Fragments arranged by size:
2961 1935 181 163
BtsI GCAGTG_nn'
Cuts at: 512 2483 512
Size: 1971 3269
ClaI AT'CG_AT
Cuts at: 1469 1469
Size: 5240
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 303 338 1356 1391 1851 1886 303
Size: 35 1018 35 460 35 3657
Fragments arranged by size:
3657 1018 460 35 35 35
DraI TTT'AAA
Cuts at: 1626 2094 2239 1626
Size: 468 145 4627
Fragments arranged by size:
4627 468 145
DraIII CAC_nnn'GTG
Cuts at: 507 507
Size: 5240
DrdI GACnn_nn'nnGTC
Cuts at: 2946 4290 2946
Size: 1344 3896
EaeI y'GGCC_r
Cuts at: 1121 1730 2825 2999 3390 3417 4762 1121
Size: 609 1095 174 391 27 1345 1599
Fragments arranged by size:
1599 1345 1095 609 391 174 27
EagI C'GGCC_G
Cuts at: 2825 2825
Size: 5240
EarI CTCTTCn'nnn_
Cuts at: 3263 3473 3263
Size: 210 5030
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3448 4190 4336 5050 3448
Size: 742 146 714 3638
Fragments arranged by size:
3638 742 714 146
EcoICRI GAG'CTC
Cuts at: 375 2252 375
Size: 1877 3363
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 895 1018 1104 1287 1312 1796 1835 2114
Size: 635 123 86 183 25 484 39 279
Cuts at: 2114 3065 3497 3850 4512 260
Size: 951 432 353 662 988
Fragments arranged by size:
988 951 662 635 484 432 353 279
183 123 86 39 25
EcoNI CCTnn'n_nnAGG
Cuts at: 637 1072 637
Size: 435 4805
EcoO109I rG'GnC_Cy
Cuts at: 750 1260 2379 750
Size: 510 1119 3611
Fragments arranged by size:
3611 1119 510
EcoRI G'AATT_C
Cuts at: 2244 2244
Size: 5240
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 633 1037 3259 633
Size: 404 2222 2614
Fragments arranged by size:
2614 2222 404
FspI TGC'GCA
Cuts at: 3021 3021
Size: 5240
HaeII r_GCGC'y
Cuts at: 2674 2922 4152 4923 2674
Size: 248 1230 771 2991
Fragments arranged by size:
2991 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 373 405 598 630 1348 1380 1443 1462 1475
Size: 32 193 32 718 32 63 19 13
Cuts at: 1475 1494 1538 1570 1882 1914 2163 2195 3082
Size: 19 44 32 312 32 249 32 887
Cuts at: 3082 3112 3114 3144 373
Size: 30 2 30 2469
Fragments arranged by size:
2469 887 718 312 249 193 63 44
32 32 32 32 32 32 30 30
19 19 13 2
HincII GTy'rAC
Cuts at: 1611 2279 1611
Size: 668 4572
HindIII A'AGCT_T
Cuts at: 272 272
Size: 5240
KasI G'GCGC_C
Cuts at: 2670 2918 2670
Size: 248 4992
KpnI G_GTAC'C
Cuts at: 1684 2260 1684
Size: 576 4664
MluI A'CGCG_T
Cuts at: 4673 4673
Size: 5240
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 540 1170 3999 4183 540
Size: 630 2829 184 1597
Fragments arranged by size:
2829 1597 630 184
MscI TGG'CCA
Cuts at: 1123 3001 1123
Size: 1878 3362
MslI CAynn'nnrTG
Cuts at: 1013 1129 3229 3356 1013
Size: 116 2100 127 2897
Fragments arranged by size:
2897 2100 127 116
MspA1I CmG'CkG
Cuts at: 203 580 901 2351 2665 3025 3809 4054 203
Size: 377 321 1450 314 360 784 245 1389
Fragments arranged by size:
1450 1389 784 377 360 321 314 245
NaeI GCC'GGC
Cuts at: 3421 3421
Size: 5240
NarI GG'CG_CC
Cuts at: 2671 2919 2671
Size: 248 4992
NcoI C'CATG_G
Cuts at: 107 107
Size: 5240
NgoMIV G'CCGG_C
Cuts at: 3419 3419
Size: 5240
NspI r_CATG'y
Cuts at: 2293 2793 4396 2293
Size: 500 1603 3137
Fragments arranged by size:
3137 1603 500
PciI A'CATG_T
Cuts at: 4392 4392
Size: 5240
PfoI T'CCnGG_A
Cuts at: 356 1289 356
Size: 933 4307
PmeI GTTT'AAAC
Cuts at: 2239 2239
Size: 5240
PmlI CAC'GTG
Cuts at: 2048 2048
Size: 5240
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3686 3718 3686
Size: 32 5208
PpuMI rG'GwC_Cy
Cuts at: 1260 1260
Size: 5240
PstI C_TGCA'G
Cuts at: 1178 2287 2972 1178
Size: 1109 685 3446
Fragments arranged by size:
3446 1109 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5240
PvuII CAG'CTG
Cuts at: 580 901 2665 3025 580
Size: 321 1764 360 2795
Fragments arranged by size:
2795 1764 360 321
RsrII CG'GwC_CG
Cuts at: 3435 3435
Size: 5240
SacI G_AGCT'C
Cuts at: 377 2254 377
Size: 1877 3363
SacII CC_GC'GG
Cuts at: 204 204
Size: 5240
SalI G'TCGA_C
Cuts at: 2277 2277
Size: 5240
SapI GCTCTTCn'nnn_
Cuts at: 3263 3473 3263
Size: 210 5030
SbfI CC_TGCA'GG
Cuts at: 2287 2287
Size: 5240
ScaI AGT'ACT
Cuts at: 4757 4757
Size: 5240
SfcI C'TryA_G
Cuts at: 33 1174 2283 2968 3936 4127 33
Size: 1141 1109 685 968 191 1146
Fragments arranged by size:
1146 1141 1109 968 685 191
SfoI GGC'GCC
Cuts at: 2672 2920 2672
Size: 248 4992
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5240
SmaI CCC'GGG
Cuts at: 2262 2262
Size: 5240
SmlI C'TyrA_G
Cuts at: 289 2395 3310 4024 4286 289
Size: 2106 915 714 262 1243
Fragments arranged by size:
2106 1243 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5240
SphI G_CATG'C
Cuts at: 2293 2793 2293
Size: 500 4740
SspI AAT'ATT
Cuts at: 1056 1056
Size: 5240
StyI C'CwwG_G
Cuts at: 107 122 1100 1676 2374 2728 107
Size: 15 978 576 698 354 2619
Fragments arranged by size:
2619 978 698 576 354 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 947 1373 1459 2823 2956 3415 3624 947
Size: 426 86 1364 133 459 209 2563
Fragments arranged by size:
2563 1364 459 426 209 133 86
TatI w'GTAC_w
Cuts at: 993 4755 993
Size: 3762 1478
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4757 4789 4757
Size: 32 5208
Tth111I GACn'n_nGTC
Cuts at: 957 1281 3037 957
Size: 324 1756 3160
Fragments arranged by size:
3160 1756 324
XbaI T'CTAG_A
Cuts at: 2271 2271
Size: 5240
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1253 2735 1253
Size: 1482 3758
XmaI C'CCGG_G
Cuts at: 2260 2260
Size: 5240
XmnI GAAnn'nnTTC
Cuts at: 1980 1980
Size: 5240
ZraI GAC'GTC
Cuts at: 624 1811 624
Size: 1187 4053
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PmlI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AgeI AleI AlfI AloI ApaI AscI
AvrII BaeI BfrBI BglI BmgBI BmtI BsiWI BsmI
BsmBI BspEI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PshAI PsiI PspOMI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI