Restriction map for
pF1KB3324
(Circular) (Six-base) MAPSORT of: pF1KB3324.seq Check: 7573 from: 1 to: 4928
pF1KB3324 4928 bp
With 182 enzymes: SgfI *
February 15, 2010 17:49 ..
AccI GT'mk_AC
Cuts at: 1403 1749 1966 1403
Size: 346 217 4365
Fragments arranged by size:
4365 346 217
Acc65I G'GTAC_C
Cuts at: 752 1944 752
Size: 1192 3736
AclI AA'CG_TT
Cuts at: 4768 4768
Size: 4928
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2753 3185 3932 4200 2753
Size: 432 747 268 3481
Fragments arranged by size:
3481 747 432 268
AflIII A'CryG_T
Cuts at: 1238 2280 3384 4361 1238
Size: 1042 1104 977 1805
Fragments arranged by size:
1805 1104 1042 977
AgeI A'CCGG_T
Cuts at: 3316 3316
Size: 4928
AhdI GACnn_n'nnGTC
Cuts at: 1745 1745
Size: 4928
AleI CACnn'nnGTG
Cuts at: 1253 1253
Size: 4928
AlwNI CAG_nnn'CTG
Cuts at: 241 365 2233 3800 241
Size: 124 1868 1567 1369
Fragments arranged by size:
1868 1567 1369 124
ApaLI G'TGCA_C
Cuts at: 3698 3698
Size: 4928
ApoI r'AATT_y
Cuts at: 264 327 559 992 1118 1477 1801 1932 4281
Size: 63 232 433 126 359 324 131 2349
Cuts at: 4281 4537 264
Size: 256 655
Fragments arranged by size:
2349 655 433 359 324 256 232 131
126 63
AseI AT'TA_AT
Cuts at: 20 20
Size: 4928
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4928
AvaI C'yCGr_G
Cuts at: 665 1948 665
Size: 1283 3645
AvrII C'CTAG_G
Cuts at: 304 304
Size: 4928
BamHI G'GATC_C
Cuts at: 1953 1953
Size: 4928
BanI G'GyrC_C
Cuts at: 752 1944 2358 2606 2641 752
Size: 1192 414 248 35 3039
Fragments arranged by size:
3039 1192 414 248 35
BanII G_rGCy'C
Cuts at: 1942 2972 1942
Size: 1030 3898
BbeI G_GCGC'C
Cuts at: 2362 2610 2362
Size: 248 4680
BbsI GAAGACnn'nnnn_
Cuts at: 287 415 981 287
Size: 128 566 4234
Fragments arranged by size:
4234 566 128
BciVI GTATCCnnnnn_n'
Cuts at: 2826 3587 4928 2826
Size: 761 1341 2826
Fragments arranged by size:
2826 1341 761
BclI T'GATC_A
Cuts at: 1044 1172 2448 1044
Size: 128 1276 3524
Fragments arranged by size:
3524 1276 128
BglII A'GATC_T
Cuts at: 1085 2443 4480 1085
Size: 1358 2037 1533
Fragments arranged by size:
2037 1533 1358
BlpI GC'TnA_GC
Cuts at: 2040 2040
Size: 4928
Bme1580I G_kGCm'C
Cuts at: 470 608 2553 2646 3702 470
Size: 138 1945 93 1056 1696
Fragments arranged by size:
1945 1696 1056 138 93
BmrI ACTGGGnnnn_n'
Cuts at: 2140 2221 2556 4700 2140
Size: 81 335 2144 2368
Fragments arranged by size:
2368 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3295 3295
Size: 4928
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 174 1138 1902 174
Size: 964 764 3200
Fragments arranged by size:
3200 964 764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 130 2104 2983 3475 3773 130
Size: 1974 879 492 298 1285
Fragments arranged by size:
1974 1285 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 176 4632 176
Size: 4456 472
BsaAI yAC'GTr
Cuts at: 2281 2281
Size: 4928
BsaBI GATnn'nnATC
Cuts at: 6 2442 4479 6
Size: 2436 2037 455
Fragments arranged by size:
2436 2037 455
BsaHI Gr'CG_yC
Cuts at: 2359 2607 3309 4805 2359
Size: 248 702 1496 2482
Fragments arranged by size:
2482 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1361 1771 2117 2638 3316 3590 3737 4148 1361
Size: 410 346 521 678 274 147 411 2141
Fragments arranged by size:
2141 678 521 411 410 346 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 884 884
Size: 4928
BseYI C'CCAG_C
Cuts at: 180 2353 3111 3688 4184 180
Size: 2173 758 577 496 924
Fragments arranged by size:
2173 924 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1252 1252
Size: 4928
BsiEI CG_ry'CG
Cuts at: 104 119 2470 2516 3724 104
Size: 15 2351 46 1208 1308
Fragments arranged by size:
2351 1308 1208 46 15
BsiHKAI G_wGCw'C
Cuts at: 160 918 1731 1942 2720 2910 3702 160
Size: 758 813 211 778 190 792 1386
Fragments arranged by size:
1386 813 792 778 758 211 190
BsmI GAATG_Cn'
Cuts at: 894 1114 1767 894
Size: 220 653 4055
Fragments arranged by size:
4055 653 220
Bsp1286I G_dGCh'C
Cuts at: 160 470 608 918 1731 1942 2553 2646 2720
Size: 310 138 310 813 211 611 93 74
Cuts at: 2720 2910 2972 3702 160
Size: 190 62 730 1386
Fragments arranged by size:
1386 813 730 611 310 310 211 190
138 93 74 62
BspEI T'CCGG_A
Cuts at: 1361 1771 4148 1361
Size: 410 2377 2141
Fragments arranged by size:
2377 2141 410
BspHI T'CATG_A
Cuts at: 4104 4104
Size: 4928
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1309 1978 2192 2494 2875 1309
Size: 669 214 302 381 3362
Fragments arranged by size:
3362 669 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3220 3274 4758 4923 50
Size: 3170 54 1484 165 55
Fragments arranged by size:
3170 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1319 1511 2840 1319
Size: 192 1329 3407
Fragments arranged by size:
3407 1329 192
BsrFI r'CCGG_y
Cuts at: 2926 3107 3316 2926
Size: 181 209 4538
Fragments arranged by size:
4538 209 181
BssSI C'ACGA_G
Cuts at: 3199 3557 3199
Size: 358 4570
BstAPI GCAn_nnn'nTGC
Cuts at: 400 2305 400
Size: 1905 3023
BstBI TT'CG_AA
Cuts at: 3289 3289
Size: 4928
BstEII G'GTnAC_C
Cuts at: 1296 1296
Size: 4928
BstYI r'GATC_y
Cuts at: 1 1085 1701 1953 2420 2443 2778 3024 4036
Size: 1084 616 252 467 23 335 246 1012
Cuts at: 4036 4122 4134 4480 1
Size: 86 12 346 449
Fragments arranged by size:
1084 1012 616 467 449 346 335 252
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 1196 107
Size: 1089 3839
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 234 2878 3059 3222 234
Size: 2644 181 163 1940
Fragments arranged by size:
2644 1940 181 163
BtsI GCAGTG_nn'
Cuts at: 398 541 996 2171 398
Size: 143 455 1175 3155
Fragments arranged by size:
3155 1175 455 143
DraI TTT'AAA
Cuts at: 1927 1927
Size: 4928
DraIII CAC_nnn'GTG
Cuts at: 724 724
Size: 4928
DrdI GACnn_nn'nnGTC
Cuts at: 2634 3492 2634
Size: 858 4070
EaeI y'GGCC_r
Cuts at: 116 425 2513 2687 3078 3105 4450 116
Size: 309 2088 174 391 27 1345 594
Fragments arranged by size:
2088 1345 594 391 309 174 27
EagI C'GGCC_G
Cuts at: 116 2513 116
Size: 2397 2531
EarI CTCTTCn'nnn_
Cuts at: 837 1770 2951 3161 837
Size: 933 1181 210 2604
Fragments arranged by size:
2604 1181 933 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 214 3136 3446 3592 4738 214
Size: 2922 310 146 1146 404
Fragments arranged by size:
2922 1146 404 310 146
EcoICRI GAG'CTC
Cuts at: 1940 1940
Size: 4928
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 174 1138 1902 2753 3185 3932 4200 174
Size: 964 764 851 432 747 268 902
Fragments arranged by size:
964 902 851 764 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1487 1673 2067 1487
Size: 186 394 4348
Fragments arranged by size:
4348 394 186
EcoRI G'AATT_C
Cuts at: 264 327 992 1118 1801 1932 264
Size: 63 665 126 683 131 3260
Fragments arranged by size:
3260 683 665 131 126 63
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2947 2947
Size: 4928
FspI TGC'GCA
Cuts at: 2709 2709
Size: 4928
HaeII r_GCGC'y
Cuts at: 2362 2610 3632 4611 2362
Size: 248 1022 979 2679
Fragments arranged by size:
2679 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 523 555 700 732 801 833 1324 1356 1376
Size: 32 145 32 69 32 491 32 20
Cuts at: 1376 1408 1731 1763 2770 2800 2802 2832 523
Size: 32 323 32 1007 30 2 30 2619
Fragments arranged by size:
2619 1007 491 323 145 69 32 32
32 32 32 32 30 30 20 2
HincII GTy'rAC
Cuts at: 1967 1967
Size: 4928
HindIII A'AGCT_T
Cuts at: 787 787
Size: 4928
KasI G'GCGC_C
Cuts at: 2358 2606 2358
Size: 248 4680
KpnI G_GTAC'C
Cuts at: 756 1948 756
Size: 1192 3736
MluI A'CGCG_T
Cuts at: 4361 4361
Size: 4928
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1356 3599 3783 1356
Size: 2243 184 2501
Fragments arranged by size:
2501 2243 184
MscI TGG'CCA
Cuts at: 427 2689 427
Size: 2262 2666
MslI CAynn'nnrTG
Cuts at: 403 1107 1253 1294 2917 3044 403
Size: 704 146 41 1623 127 2287
Fragments arranged by size:
2287 1623 704 146 127 41
MspA1I CmG'CkG
Cuts at: 353 1867 2039 2353 2713 3726 3971 353
Size: 1514 172 314 360 1013 245 1310
Fragments arranged by size:
1514 1310 1013 360 314 245 172
NaeI GCC'GGC
Cuts at: 3109 3109
Size: 4928
NarI GG'CG_CC
Cuts at: 2359 2607 2359
Size: 248 4680
NcoI C'CATG_G
Cuts at: 107 107
Size: 4928
NgoMIV G'CCGG_C
Cuts at: 3107 3107
Size: 4928
NspI r_CATG'y
Cuts at: 210 777 1601 1981 2481 3388 210
Size: 567 824 380 500 907 1750
Fragments arranged by size:
1750 907 824 567 500 380
PasI CC'CwG_GG
Cuts at: 235 235
Size: 4928
PciI A'CATG_T
Cuts at: 3384 3384
Size: 4928
PflMI CCAn_nnn'nTGG
Cuts at: 241 241
Size: 4928
PmeI GTTT'AAAC
Cuts at: 1927 1927
Size: 4928
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4067 4099 4067
Size: 32 4896
PpuMI rG'GwC_Cy
Cuts at: 1487 1487
Size: 4928
PstI C_TGCA'G
Cuts at: 1737 1975 2660 1737
Size: 238 685 4005
Fragments arranged by size:
4005 685 238
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4928
PvuII CAG'CTG
Cuts at: 353 1867 2353 2713 353
Size: 1514 486 360 2568
Fragments arranged by size:
2568 1514 486 360
RsrII CG'GwC_CG
Cuts at: 3123 3123
Size: 4928
SacI G_AGCT'C
Cuts at: 1942 1942
Size: 4928
SalI G'TCGA_C
Cuts at: 1965 1965
Size: 4928
SapI GCTCTTCn'nnn_
Cuts at: 2951 3161 2951
Size: 210 4718
SbfI CC_TGCA'GG
Cuts at: 1737 1975 1737
Size: 238 4690
ScaI AGT'ACT
Cuts at: 1470 4445 1470
Size: 2975 1953
SfcI C'TryA_G
Cuts at: 33 1733 1789 1971 2656 3649 3840 33
Size: 1700 56 182 685 993 191 1121
Fragments arranged by size:
1700 1121 993 685 191 182 56
SfoI GGC'GCC
Cuts at: 2360 2608 2360
Size: 248 4680
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4928
SmaI CCC'GGG
Cuts at: 1950 1950
Size: 4928
SmlI C'TyrA_G
Cuts at: 145 2083 2998 3490 3752 145
Size: 1938 915 492 262 1321
Fragments arranged by size:
1938 1321 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4928
SphI G_CATG'C
Cuts at: 210 777 1981 2481 210
Size: 567 1204 500 2657
Fragments arranged by size:
2657 1204 567 500
StuI AGG'CCT
Cuts at: 830 830
Size: 4928
StyI C'CwwG_G
Cuts at: 107 304 2062 2416 107
Size: 197 1758 354 2619
Fragments arranged by size:
2619 1758 354 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2511 2644 3103 3312 2511
Size: 133 459 209 4127
Fragments arranged by size:
4127 459 209 133
TatI w'GTAC_w
Cuts at: 1418 1468 1526 4443 1418
Size: 50 58 2917 1903
Fragments arranged by size:
2917 1903 58 50
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1653 1685 4445 4477 1653
Size: 32 2760 32 2104
Fragments arranged by size:
2760 2104 32 32
Tth111I GACn'n_nGTC
Cuts at: 2725 2725
Size: 4928
XbaI T'CTAG_A
Cuts at: 1959 1959
Size: 4928
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2423 2423
Size: 4928
XmaI C'CCGG_G
Cuts at: 1948 1948
Size: 4928
XmnI GAAnn'nnTTC
Cuts at: 1262 1262
Size: 4928
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI ApaI AscI
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI