Restriction map for
pF1KB3331
(Circular) (Six-base) MAPSORT of: pF1KB3331.seq Check: 6304 from: 1 to: 4655
pF1KB3331 4655 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 1571 1693 1571
Size: 122 4533
Acc65I G'GTAC_C
Cuts at: 1671 1671
Size: 4655
AclI AA'CG_TT
Cuts at: 4495 4495
Size: 4655
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1568 2480 2912 3659 3927 1568
Size: 912 432 747 268 2296
Fragments arranged by size:
2296 912 747 432 268
AflIII A'CryG_T
Cuts at: 176 2007 3111 4088 176
Size: 1831 1104 977 743
Fragments arranged by size:
1831 1104 977 743
AgeI A'CCGG_T
Cuts at: 3043 3043
Size: 4655
AleI CACnn'nnGTG
Cuts at: 145 145
Size: 4655
AlwNI CAG_nnn'CTG
Cuts at: 418 1960 3527 418
Size: 1542 1567 1546
Fragments arranged by size:
1567 1546 1542
ApaLI G'TGCA_C
Cuts at: 3425 3425
Size: 4655
ApoI r'AATT_y
Cuts at: 594 1303 1371 1659 4008 4264 594
Size: 709 68 288 2349 256 985
Fragments arranged by size:
2349 985 709 288 256 68
AseI AT'TA_AT
Cuts at: 20 20
Size: 4655
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4655
AvaI C'yCGr_G
Cuts at: 1675 1675
Size: 4655
BamHI G'GATC_C
Cuts at: 1680 1680
Size: 4655
BanI G'GyrC_C
Cuts at: 677 1408 1671 2085 2333 2368 677
Size: 731 263 414 248 35 2964
Fragments arranged by size:
2964 731 414 263 248 35
BanII G_rGCy'C
Cuts at: 883 1669 2699 883
Size: 786 1030 2839
Fragments arranged by size:
2839 1030 786
BbeI G_GCGC'C
Cuts at: 2089 2337 2089
Size: 248 4407
BbsI GAAGACnn'nnnn_
Cuts at: 911 911
Size: 4655
BciVI GTATCCnnnnn_n'
Cuts at: 2553 3314 4655 2553
Size: 761 1341 2553
Fragments arranged by size:
2553 1341 761
BclI T'GATC_A
Cuts at: 1518 2175 1518
Size: 657 3998
BfrBI ATG'CAT
Cuts at: 1416 1416
Size: 4655
BglII A'GATC_T
Cuts at: 794 2170 4207 794
Size: 1376 2037 1242
Fragments arranged by size:
2037 1376 1242
BlpI GC'TnA_GC
Cuts at: 1767 1767
Size: 4655
Bme1580I G_kGCm'C
Cuts at: 2280 2373 3429 2280
Size: 93 1056 3506
Fragments arranged by size:
3506 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 666 845 1867 1948 2283 4427 666
Size: 179 1022 81 335 2144 894
Fragments arranged by size:
2144 1022 894 335 179 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3022 3022
Size: 4655
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1265 1415 1449 1265
Size: 150 34 4471
Fragments arranged by size:
4471 150 34
Bpu10I CC'TnA_GC
Cuts at: 483 483
Size: 4655
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1831 2710 3202 3500 1831
Size: 879 492 298 2986
Fragments arranged by size:
2986 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4359 4359
Size: 4655
BsaAI yAC'GTr
Cuts at: 2008 2008
Size: 4655
BsaBI GATnn'nnATC
Cuts at: 6 521 2169 4206 6
Size: 515 1648 2037 455
Fragments arranged by size:
2037 1648 515 455
BsaHI Gr'CG_yC
Cuts at: 2086 2334 3036 4532 2086
Size: 248 702 1496 2209
Fragments arranged by size:
2209 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1336 1844 2365 3043 3317 3464 3875 1336
Size: 508 521 678 274 147 411 2116
Fragments arranged by size:
2116 678 521 508 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1373 1403 1373
Size: 30 4625
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 130 130
Size: 4655
BseYI C'CCAG_C
Cuts at: 722 923 2080 2838 3415 3911 722
Size: 201 1157 758 577 496 1466
Fragments arranged by size:
1466 1157 758 577 496 201
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 458 617 995 1025 458
Size: 159 378 30 4088
Fragments arranged by size:
4088 378 159 30
BsiEI CG_ry'CG
Cuts at: 104 2197 2243 3451 104
Size: 2093 46 1208 1308
Fragments arranged by size:
2093 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1669 2447 2637 3429 1669
Size: 778 190 792 2895
Fragments arranged by size:
2895 792 778 190
BsmI GAATG_Cn'
Cuts at: 327 461 327
Size: 134 4521
Bsp1286I G_dGCh'C
Cuts at: 883 1669 2280 2373 2447 2637 2699 3429 883
Size: 786 611 93 74 190 62 730 2109
Fragments arranged by size:
2109 786 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3875 3875
Size: 4655
BspHI T'CATG_A
Cuts at: 3831 3831
Size: 4655
BspMI ACCTGCnnnn'nnnn_
Cuts at: 382 1705 1919 2221 2602 382
Size: 1323 214 302 381 2435
Fragments arranged by size:
2435 1323 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2947 3001 4485 4650 50
Size: 2897 54 1484 165 55
Fragments arranged by size:
2897 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2567 2567
Size: 4655
BsrFI r'CCGG_y
Cuts at: 2653 2834 3043 2653
Size: 181 209 4265
Fragments arranged by size:
4265 209 181
BsrGI T'GTAC_A
Cuts at: 135 1074 135
Size: 939 3716
BssSI C'ACGA_G
Cuts at: 2926 3284 2926
Size: 358 4297
BstAPI GCAn_nnn'nTGC
Cuts at: 2032 2032
Size: 4655
BstBI TT'CG_AA
Cuts at: 3016 3016
Size: 4655
BstEII G'GTnAC_C
Cuts at: 1017 1017
Size: 4655
BstXI CCAn_nnnn'nTGG
Cuts at: 1616 1616
Size: 4655
BstYI r'GATC_y
Cuts at: 1 446 772 794 927 1179 1680 2147 2170
Size: 445 326 22 133 252 501 467 23
Cuts at: 2170 2505 2751 3763 3849 3861 4207 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 501 467 449 445 346 335 326
252 246 133 86 23 22 12
BstZ17I GTA'TAC
Cuts at: 1572 1572
Size: 4655
BtgI C'CryG_G
Cuts at: 107 685 107
Size: 578 4077
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 604 2605 2786 2949 604
Size: 2001 181 163 2310
Fragments arranged by size:
2310 2001 181 163
BtsI GCAGTG_nn'
Cuts at: 983 1303 1898 983
Size: 320 595 3740
Fragments arranged by size:
3740 595 320
ClaI AT'CG_AT
Cuts at: 1007 1007
Size: 4655
DraI TTT'AAA
Cuts at: 1654 1654
Size: 4655
DrdI GACnn_nn'nnGTC
Cuts at: 2361 3219 2361
Size: 858 3797
EaeI y'GGCC_r
Cuts at: 2240 2414 2805 2832 4177 2240
Size: 174 391 27 1345 2718
Fragments arranged by size:
2718 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2240 2240
Size: 4655
EarI CTCTTCn'nnn_
Cuts at: 157 212 2678 2888 157
Size: 55 2466 210 1924
Fragments arranged by size:
2466 1924 210 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2863 3173 3319 4465 2863
Size: 310 146 1146 3053
Fragments arranged by size:
3053 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1667 1667
Size: 4655
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1265 1415 1449 1568 2480 2912 3659 3927 1265
Size: 150 34 119 912 432 747 268 1993
Fragments arranged by size:
1993 912 747 432 268 150 119 34
EcoO109I rG'GnC_Cy
Cuts at: 167 313 415 1794 167
Size: 146 102 1379 3028
Fragments arranged by size:
3028 1379 146 102
EcoRI G'AATT_C
Cuts at: 594 1659 594
Size: 1065 3590
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 316 2674 316
Size: 2358 2297
FspI TGC'GCA
Cuts at: 2436 2436
Size: 4655
HaeII r_GCGC'y
Cuts at: 2089 2337 3359 4338 2089
Size: 248 1022 979 2406
Fragments arranged by size:
2406 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 870 902 1477 1509 2497 2527 2529 2559 870
Size: 32 575 32 988 30 2 30 2966
Fragments arranged by size:
2966 988 575 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1694 1694
Size: 4655
HindIII A'AGCT_T
Cuts at: 247 247
Size: 4655
KasI G'GCGC_C
Cuts at: 2085 2333 2085
Size: 248 4407
KpnI G_GTAC'C
Cuts at: 1675 1675
Size: 4655
MfeI C'AATT_G
Cuts at: 599 599
Size: 4655
MluI A'CGCG_T
Cuts at: 4088 4088
Size: 4655
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3326 3510 3326
Size: 184 4471
MscI TGG'CCA
Cuts at: 2416 2416
Size: 4655
MslI CAynn'nnrTG
Cuts at: 145 959 971 1097 2644 2771 145
Size: 814 12 126 1547 127 2029
Fragments arranged by size:
2029 1547 814 127 126 12
MspA1I CmG'CkG
Cuts at: 472 1465 1546 1616 1766 2080 2440 3453 3698
Size: 993 81 70 150 314 360 1013 245
Cuts at: 3698 472
Size: 1429
Fragments arranged by size:
1429 1013 993 360 314 245 150 81
70
NaeI GCC'GGC
Cuts at: 2836 2836
Size: 4655
NarI GG'CG_CC
Cuts at: 2086 2334 2086
Size: 248 4407
NcoI C'CATG_G
Cuts at: 107 107
Size: 4655
NdeI CA'TA_TG
Cuts at: 278 336 278
Size: 58 4597
NgoMIV G'CCGG_C
Cuts at: 2834 2834
Size: 4655
NsiI A_TGCA'T
Cuts at: 1418 1418
Size: 4655
NspI r_CATG'y
Cuts at: 180 1708 2208 3115 180
Size: 1528 500 907 1720
Fragments arranged by size:
1720 1528 907 500
PciI A'CATG_T
Cuts at: 176 3111 176
Size: 2935 1720
PflMI CCAn_nnn'nTGG
Cuts at: 418 418
Size: 4655
PmeI GTTT'AAAC
Cuts at: 1654 1654
Size: 4655
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3794 3826 3794
Size: 32 4623
PpuMI rG'GwC_Cy
Cuts at: 167 313 415 167
Size: 146 102 4407
Fragments arranged by size:
4407 146 102
PstI C_TGCA'G
Cuts at: 1131 1702 2387 1131
Size: 571 685 3399
Fragments arranged by size:
3399 685 571
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4655
PvuII CAG'CTG
Cuts at: 472 1465 1546 1616 2080 2440 472
Size: 993 81 70 464 360 2687
Fragments arranged by size:
2687 993 464 360 81 70
RsrII CG'GwC_CG
Cuts at: 2850 2850
Size: 4655
SacI G_AGCT'C
Cuts at: 1669 1669
Size: 4655
SalI G'TCGA_C
Cuts at: 1692 1692
Size: 4655
SapI GCTCTTCn'nnn_
Cuts at: 2678 2888 2678
Size: 210 4445
SbfI CC_TGCA'GG
Cuts at: 1702 1702
Size: 4655
ScaI AGT'ACT
Cuts at: 849 4172 849
Size: 3323 1332
SfcI C'TryA_G
Cuts at: 33 584 696 1023 1109 1127 1698 2383 3376
Size: 551 112 327 86 18 571 685 993
Cuts at: 3376 3567 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 571 551 327 191 112
86 18
SfoI GGC'GCC
Cuts at: 2087 2335 2087
Size: 248 4407
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4655
SmaI CCC'GGG
Cuts at: 1677 1677
Size: 4655
SmlI C'TyrA_G
Cuts at: 1810 2725 3217 3479 1810
Size: 915 492 262 2986
Fragments arranged by size:
2986 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4655
SphI G_CATG'C
Cuts at: 1708 2208 1708
Size: 500 4155
SspI AAT'ATT
Cuts at: 1502 1502
Size: 4655
StyI C'CwwG_G
Cuts at: 107 509 1789 2143 107
Size: 402 1280 354 2619
Fragments arranged by size:
2619 1280 402 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 695 2238 2371 2830 3039 695
Size: 1543 133 459 209 2311
Fragments arranged by size:
2311 1543 459 209 133
TatI w'GTAC_w
Cuts at: 135 847 1074 4170 135
Size: 712 227 3096 620
Fragments arranged by size:
3096 712 620 227
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 188 220 4172 4204 188
Size: 32 3952 32 639
Fragments arranged by size:
3952 639 32 32
Tth111I GACn'n_nGTC
Cuts at: 2452 2452
Size: 4655
XbaI T'CTAG_A
Cuts at: 1686 1686
Size: 4655
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 671 2150 671
Size: 1479 3176
XmaI C'CCGG_G
Cuts at: 1675 1675
Size: 4655
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PciI PflMI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI
BsiWI BsmBI BssHII Bsu36I CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI NheI NotI NruI PacI PasI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI