Restriction map for
pF1KB3334
(Circular) (Six-base) MAPSORT of: pF1KB3334.seq Check: 4550 from: 1 to: 5423
pF1KB3334 5423 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AatII G_ACGT'C
Cuts at: 1835 1835
Size: 5423
AccI GT'mk_AC
Cuts at: 335 2461 335
Size: 2126 3297
Acc65I G'GTAC_C
Cuts at: 165 2439 165
Size: 2274 3149
AclI AA'CG_TT
Cuts at: 2417 5263 2417
Size: 2846 2577
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 698 1064 1789 2129 2296 3248 3680 4033 4695
Size: 366 725 340 167 952 432 353 662
Cuts at: 4695 698
Size: 1426
Fragments arranged by size:
1426 952 725 662 432 366 353 340
167
AflIII A'CryG_T
Cuts at: 1574 2775 4575 4856 1574
Size: 1201 1800 281 2141
Fragments arranged by size:
2141 1800 1201 281
AleI CACnn'nnGTG
Cuts at: 358 1709 2266 358
Size: 1351 557 3515
Fragments arranged by size:
3515 1351 557
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 637 637
Size: 5423
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2138 2170 2138
Size: 32 5391
AlwNI CAG_nnn'CTG
Cuts at: 952 1464 2074 2728 4166 952
Size: 512 610 654 1438 2209
Fragments arranged by size:
2209 1438 654 610 512
ApaLI G'TGCA_C
Cuts at: 4261 4261
Size: 5423
ApoI r'AATT_y
Cuts at: 508 2128 2427 4776 5032 508
Size: 1620 299 2349 256 899
Fragments arranged by size:
2349 1620 899 299 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5423
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5423
AvaI C'yCGr_G
Cuts at: 152 1181 1243 2443 152
Size: 1029 62 1200 3132
Fragments arranged by size:
3132 1200 1029 62
BamHI G'GATC_C
Cuts at: 1662 2448 1662
Size: 786 4637
BanI G'GyrC_C
Cuts at: 165 2439 2853 3101 3136 165
Size: 2274 414 248 35 2452
Fragments arranged by size:
2452 2274 414 248 35
BanII G_rGCy'C
Cuts at: 1925 2437 3467 1925
Size: 512 1030 3881
Fragments arranged by size:
3881 1030 512
BbeI G_GCGC'C
Cuts at: 2857 3105 2857
Size: 248 5175
BbsI GAAGACnn'nnnn_
Cuts at: 779 779
Size: 5423
BbvCI CC'TCA_GC
Cuts at: 812 881 812
Size: 69 5354
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 959 993 90
Size: 34 835 34 4520
Fragments arranged by size:
4520 835 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3321 4377 5423 3321
Size: 1056 1046 3321
Fragments arranged by size:
3321 1056 1046
BclI T'GATC_A
Cuts at: 2943 2943
Size: 5423
BglI GCCn_nnn'nGGC
Cuts at: 148 148
Size: 5423
BglII A'GATC_T
Cuts at: 2102 2938 4975 2102
Size: 836 2037 2550
Fragments arranged by size:
2550 2037 836
BlpI GC'TnA_GC
Cuts at: 2535 2535
Size: 5423
Bme1580I G_kGCm'C
Cuts at: 1059 3048 3141 4265 1059
Size: 1989 93 1124 2217
Fragments arranged by size:
2217 1989 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 2635 2716 3051 5195 2635
Size: 81 335 2144 2863
Fragments arranged by size:
2863 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 738 996 2121 3790 738
Size: 258 1125 1669 2371
Fragments arranged by size:
2371 1669 1125 258
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 1546 1945 181
Size: 1365 399 3659
Fragments arranged by size:
3659 1365 399
Bpu10I CC'TnA_GC
Cuts at: 812 881 812
Size: 69 5354
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1141 1321 2599 3478 4192 4490 1141
Size: 180 1278 879 714 298 2074
Fragments arranged by size:
2074 1278 879 714 298 180
BsaI GGTCTCn'nnnn_
Cuts at: 5127 5127
Size: 5423
BsaAI yAC'GTr
Cuts at: 1575 2776 1575
Size: 1201 4222
BsaBI GATnn'nnATC
Cuts at: 6 269 2257 2937 4974 6
Size: 263 1988 680 2037 455
Fragments arranged by size:
2037 1988 680 455 263
BsaHI Gr'CG_yC
Cuts at: 1832 2324 2854 3102 3804 5300 1832
Size: 492 530 248 702 1496 1955
Fragments arranged by size:
1955 1496 702 530 492 248
BsaWI w'CCGG_w
Cuts at: 1088 1685 2612 3133 3811 4222 4369 4643 1088
Size: 597 927 521 678 411 147 274 1868
Fragments arranged by size:
1868 927 678 597 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 727 811 1550 1789 2113 2356 727
Size: 84 739 239 324 243 3794
Fragments arranged by size:
3794 739 324 243 239 84
BseYI C'CCAG_C
Cuts at: 2848 3606 4271 4679 2848
Size: 758 665 408 3592
Fragments arranged by size:
3592 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2049 2965 3011 4241 104
Size: 1945 916 46 1230 1286
Fragments arranged by size:
1945 1286 1230 916 46
BsiHKAI G_wGCw'C
Cuts at: 982 1925 2437 3215 3405 4265 982
Size: 943 512 778 190 860 2140
Fragments arranged by size:
2140 943 860 778 512 190
BsmI GAATG_Cn'
Cuts at: 1971 1971
Size: 5423
BsmBI CGTCTCn'nnnn_
Cuts at: 988 988
Size: 5423
Bsp1286I G_dGCh'C
Cuts at: 982 1059 1925 2437 3048 3141 3215 3405 3467
Size: 77 866 512 611 93 74 190 62
Cuts at: 3467 4265 982
Size: 798 2140
Fragments arranged by size:
2140 866 798 611 512 190 93 77
74 62
BspHI T'CATG_A
Cuts at: 1334 3855 1334
Size: 2521 2902
BspMI ACCTGCnnnn'nnnn_
Cuts at: 298 2473 2687 2989 3370 298
Size: 2175 214 302 381 2351
Fragments arranged by size:
2351 2175 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1543 1678 3715 3769 5253 5418 50
Size: 1493 135 2037 54 1484 165 55
Fragments arranged by size:
2037 1493 1484 165 135 55 54
BsrDI GCAATG_nn'
Cuts at: 920 1019 3335 920
Size: 99 2316 3008
Fragments arranged by size:
3008 2316 99
BsrFI r'CCGG_y
Cuts at: 3421 3602 3421
Size: 181 5242
BssSI C'ACGA_G
Cuts at: 3694 4402 3694
Size: 708 4715
BstAPI GCAn_nnn'nTGC
Cuts at: 419 2800 419
Size: 2381 3042
BstBI TT'CG_AA
Cuts at: 3784 3784
Size: 5423
BstYI r'GATC_y
Cuts at: 1 270 1185 1662 1682 2102 2448 2915 2938
Size: 269 915 477 20 420 346 467 23
Cuts at: 2938 3273 3519 3825 3837 3923 4975 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 915 477 467 449 420 346 335
306 269 246 86 23 20 12
BtgI C'CryG_G
Cuts at: 1916 1966 1916
Size: 50 5373
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3373 3554 3717 3373
Size: 181 163 5079
Fragments arranged by size:
5079 181 163
BtsI GCAGTG_nn'
Cuts at: 124 424 588 1772 2666 124
Size: 300 164 1184 894 2881
Fragments arranged by size:
2881 1184 894 300 164
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 903 938 903
Size: 35 5388
DraI TTT'AAA
Cuts at: 2422 2422
Size: 5423
DraIII CAC_nnn'GTG
Cuts at: 441 441
Size: 5423
DrdI GACnn_nn'nnGTC
Cuts at: 727 2329 3129 4473 727
Size: 1602 800 1344 1677
Fragments arranged by size:
1677 1602 1344 800
EaeI y'GGCC_r
Cuts at: 137 1121 1481 1769 1838 3008 3182 3573 3600
Size: 984 360 288 69 1170 174 391 27
Cuts at: 3600 4945 137
Size: 1345 615
Fragments arranged by size:
1345 1170 984 615 391 360 288 174
69 27
EagI C'GGCC_G
Cuts at: 3008 3008
Size: 5423
EarI CTCTTCn'nnn_
Cuts at: 583 869 953 1103 1343 3446 3656 583
Size: 286 84 150 240 2103 210 2350
Fragments arranged by size:
2350 2103 286 240 210 150 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 755 1088 3631 4373 4519 5233 755
Size: 333 2543 742 146 714 945
Fragments arranged by size:
2543 945 742 714 333 146
EcoICRI GAG'CTC
Cuts at: 1923 2435 1923
Size: 512 4911
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 698 1064 1546 1789 1945 2129 2296 3248
Size: 517 366 482 243 156 184 167 952
Cuts at: 3248 3680 4033 4695 181
Size: 432 353 662 909
Fragments arranged by size:
952 909 662 517 482 432 366 353
243 184 167 156
EcoO109I rG'GnC_Cy
Cuts at: 2562 2562
Size: 5423
EcoRI G'AATT_C
Cuts at: 2128 2427 2128
Size: 299 5124
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1315 3442 1315
Size: 2127 3296
FspI TGC'GCA
Cuts at: 1654 3204 1654
Size: 1550 3873
FspAI rTGC'GCAy
Cuts at: 1654 1654
Size: 5423
HaeII r_GCGC'y
Cuts at: 160 1139 2349 2857 3105 4335 5106 160
Size: 979 1210 508 248 1230 771 477
Fragments arranged by size:
1230 1210 979 771 508 477 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1001 1033 1309 1341 3265 3295 3297 3327 1001
Size: 32 276 32 1924 30 2 30 3097
Fragments arranged by size:
3097 1924 276 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1233 2081 2322 2462 1233
Size: 848 241 140 4194
Fragments arranged by size:
4194 848 241 140
KasI G'GCGC_C
Cuts at: 2853 3101 2853
Size: 248 5175
KpnI G_GTAC'C
Cuts at: 169 2443 169
Size: 2274 3149
MluI A'CGCG_T
Cuts at: 4856 4856
Size: 5423
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2157 2410 4182 4366 2157
Size: 253 1772 184 3214
Fragments arranged by size:
3214 1772 253 184
MscI TGG'CCA
Cuts at: 1483 1840 3184 1483
Size: 357 1344 3722
Fragments arranged by size:
3722 1344 357
MslI CAynn'nnrTG
Cuts at: 238 242 358 1123 1709 2266 2306 3412 3539
Size: 4 116 765 586 557 40 1106 127
Cuts at: 3539 238
Size: 2122
Fragments arranged by size:
2122 1106 765 586 557 127 116 40
4
MspA1I CmG'CkG
Cuts at: 455 1025 1464 2329 2534 2848 3208 3992 4237
Size: 570 439 865 205 314 360 784 245
Cuts at: 4237 455
Size: 1641
Fragments arranged by size:
1641 865 784 570 439 360 314 245
205
NaeI GCC'GGC
Cuts at: 3604 3604
Size: 5423
NarI GG'CG_CC
Cuts at: 2854 3102 2854
Size: 248 5175
NgoMIV G'CCGG_C
Cuts at: 3602 3602
Size: 5423
NspI r_CATG'y
Cuts at: 237 581 618 1273 1651 2476 2976 4579 237
Size: 344 37 655 378 825 500 1603 1081
Fragments arranged by size:
1603 1081 825 655 500 378 344 37
PciI A'CATG_T
Cuts at: 4575 4575
Size: 5423
PflMI CCAn_nnn'nTGG
Cuts at: 2074 2074
Size: 5423
PfoI T'CCnGG_A
Cuts at: 1412 1412
Size: 5423
PmeI GTTT'AAAC
Cuts at: 2422 2422
Size: 5423
PmlI CAC'GTG
Cuts at: 1575 1575
Size: 5423
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3869 3901 3869
Size: 32 5391
PstI C_TGCA'G
Cuts at: 320 350 1550 1767 2470 3155 320
Size: 30 1200 217 703 685 2588
Fragments arranged by size:
2588 1200 703 685 217 30
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5423
PvuII CAG'CTG
Cuts at: 1464 2848 3208 1464
Size: 1384 360 3679
Fragments arranged by size:
3679 1384 360
RsrII CG'GwC_CG
Cuts at: 3618 3618
Size: 5423
SacI G_AGCT'C
Cuts at: 1925 2437 1925
Size: 512 4911
SalI G'TCGA_C
Cuts at: 2460 2460
Size: 5423
SapI GCTCTTCn'nnn_
Cuts at: 3446 3656 3446
Size: 210 5213
SbfI CC_TGCA'GG
Cuts at: 2470 2470
Size: 5423
ScaI AGT'ACT
Cuts at: 1114 4940 1114
Size: 3826 1597
SexAI A'CCwGG_T
Cuts at: 2067 2067
Size: 5423
SfcI C'TryA_G
Cuts at: 33 316 346 1546 1763 2466 3151 4119 4310
Size: 283 30 1200 217 703 685 968 191
Cuts at: 4310 33
Size: 1146
Fragments arranged by size:
1200 1146 968 703 685 283 217 191
30
SfoI GGC'GCC
Cuts at: 2855 3103 2855
Size: 248 5175
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5423
SmaI CCC'GGG
Cuts at: 2445 2445
Size: 5423
SmlI C'TyrA_G
Cuts at: 1156 1243 1300 2578 3493 4207 4469 1156
Size: 87 57 1278 915 714 262 2110
Fragments arranged by size:
2110 1278 915 714 262 87 57
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5423
SphI G_CATG'C
Cuts at: 2476 2976 2476
Size: 500 4923
StyI C'CwwG_G
Cuts at: 302 1858 2264 2557 2911 302
Size: 1556 406 293 354 2814
Fragments arranged by size:
2814 1556 406 354 293
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1915 2063 3006 3139 3598 3807 1915
Size: 148 943 133 459 209 3531
Fragments arranged by size:
3531 943 459 209 148 133
TatI w'GTAC_w
Cuts at: 1112 2288 4938 1112
Size: 1176 2650 1597
Fragments arranged by size:
2650 1597 1176
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 718 750 2210 2242 4940 4972 718
Size: 32 1460 32 2698 32 1169
Fragments arranged by size:
2698 1460 1169 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3220 3220
Size: 5423
XbaI T'CTAG_A
Cuts at: 2454 2454
Size: 5423
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 951 2918 951
Size: 1967 3456
XhoI C'TCGA_G
Cuts at: 1243 1243
Size: 5423
XmaI C'CCGG_G
Cuts at: 2443 2443
Size: 5423
ZraI GAC'GTC
Cuts at: 1833 1833
Size: 5423
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AleI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PfoI PmeI PmlI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI ApaI AscI AvrII
BaeI BfrBI BmgBI BmtI BsaXI BsgI BsiWI BspEI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI EcoNI
EcoRV FseI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XmnI