Restriction map for
pF1KB3338
(Circular) (Six-base) MAPSORT of: pF1KB3338.seq Check: 2286 from: 1 to: 6596
pF1KB3338 6596 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2518 2518
Size: 6596
AatII G_ACGT'C
Cuts at: 3194 3194
Size: 6596
AccI GT'mk_AC
Cuts at: 1763 2870 3398 3634 1763
Size: 1107 528 236 4725
Fragments arranged by size:
4725 1107 528 236
Acc65I G'GTAC_C
Cuts at: 3612 3612
Size: 6596
AclI AA'CG_TT
Cuts at: 6436 6436
Size: 6596
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 715 1155 2263 3186 3253 3548 4421 4853 5600
Size: 440 1108 923 67 295 873 432 747
Cuts at: 5600 5868 715
Size: 268 1443
Fragments arranged by size:
1443 1108 923 873 747 440 432 295
268 67
AflIII A'CryG_T
Cuts at: 368 1040 1280 3948 5052 6029 368
Size: 672 240 2668 1104 977 935
Fragments arranged by size:
2668 1104 977 935 672 240
AgeI A'CCGG_T
Cuts at: 2015 4984 2015
Size: 2969 3627
AleI CACnn'nnGTG
Cuts at: 1417 3322 1417
Size: 1905 4691
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1027 1059 1027
Size: 32 6564
AlwNI CAG_nnn'CTG
Cuts at: 446 747 1005 1362 1866 2343 2475 2684 3901
Size: 301 258 357 504 477 132 209 1217
Cuts at: 3901 5468 446
Size: 1567 1574
Fragments arranged by size:
1574 1567 1217 504 477 357 301 258
209 132
ApaI G_GGCC'C
Cuts at: 843 1377 1545 1734 1923 843
Size: 534 168 189 189 5516
Fragments arranged by size:
5516 534 189 189 168
ApaLI G'TGCA_C
Cuts at: 5366 5366
Size: 6596
ApoI r'AATT_y
Cuts at: 3600 5949 6205 3600
Size: 2349 256 3991
Fragments arranged by size:
3991 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6596
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6596
AvaI C'yCGr_G
Cuts at: 732 1565 1734 1922 3616 732
Size: 833 169 188 1694 3712
Fragments arranged by size:
3712 1694 833 188 169
AvrII C'CTAG_G
Cuts at: 1597 1597
Size: 6596
BamHI G'GATC_C
Cuts at: 753 3621 753
Size: 2868 3728
BanI G'GyrC_C
Cuts at: 1237 2725 3612 4026 4274 4309 1237
Size: 1488 887 414 248 35 3524
Fragments arranged by size:
3524 1488 887 414 248 35
BanII G_rGCy'C
Cuts at: 493 843 1267 1377 1545 1734 1923 2284 2324
Size: 350 424 110 168 189 189 361 40
Cuts at: 2324 2368 2491 2501 2604 2719 2852 3366 3441
Size: 44 123 10 103 115 133 514 75
Cuts at: 3441 3610 4640 493
Size: 169 1030 2449
Fragments arranged by size:
2449 1030 514 424 361 350 189 189
169 168 133 123 115 110 103 75
44 40 10
BbeI G_GCGC'C
Cuts at: 4030 4278 4030
Size: 248 6348
BbsI GAAGACnn'nnnn_
Cuts at: 555 3395 3536 555
Size: 2840 141 3615
Fragments arranged by size:
3615 2840 141
BbvCI CC'TCA_GC
Cuts at: 2452 2977 2452
Size: 525 6071
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2561 2595 2561
Size: 34 6562
BciVI GTATCCnnnnn_n'
Cuts at: 1259 4494 5255 6596 1259
Size: 3235 761 1341 1259
Fragments arranged by size:
3235 1341 1259 761
BclI T'GATC_A
Cuts at: 2879 4116 2879
Size: 1237 5359
BglI GCCn_nnn'nGGC
Cuts at: 1729 1918 1729
Size: 189 6407
BglII A'GATC_T
Cuts at: 1100 2988 4111 6148 1100
Size: 1888 1123 2037 1548
Fragments arranged by size:
2037 1888 1548 1123
BlpI GC'TnA_GC
Cuts at: 1020 2951 3708 1020
Size: 1931 757 3908
Fragments arranged by size:
3908 1931 757
Bme1580I G_kGCm'C
Cuts at: 655 843 1377 1545 1734 1923 2043 2628 2654
Size: 188 534 168 189 189 120 585 26
Cuts at: 2654 4221 4314 5370 655
Size: 1567 93 1056 1881
Fragments arranged by size:
1881 1567 1056 585 534 189 189 188
168 120 93 26
BmgBI CAC'GTC
Cuts at: 1719 1719
Size: 6596
BmrI ACTGGGnnnn_n'
Cuts at: 1116 2030 2230 3808 3889 4224 6368 1116
Size: 914 200 1578 81 335 2144 1344
Fragments arranged by size:
2144 1578 1344 914 335 200 81
BmtI G_CTAG'C
Cuts at: 1339 1339
Size: 6596
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3119 4963 3119
Size: 1844 4752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 1253 2515 2866 3535 286
Size: 967 1262 351 669 3347
Fragments arranged by size:
3347 1262 967 669 351
Bpu10I CC'TnA_GC
Cuts at: 1942 2452 2977 1942
Size: 510 525 5561
Fragments arranged by size:
5561 525 510
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3772 4651 5143 5441 3772
Size: 879 492 298 4927
Fragments arranged by size:
4927 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 6300 6300
Size: 6596
BsaAI yAC'GTr
Cuts at: 1041 3949 1041
Size: 2908 3688
BsaBI GATnn'nnATC
Cuts at: 6 556 4110 6147 6
Size: 550 3554 2037 455
Fragments arranged by size:
3554 2037 550 455
BsaHI Gr'CG_yC
Cuts at: 524 2057 3191 4027 4275 4977 6473 524
Size: 1533 1134 836 248 702 1496 647
Fragments arranged by size:
1533 1496 1134 836 702 647 248
BsaWI w'CCGG_w
Cuts at: 722 2015 3785 4306 4984 5258 5405 5816 722
Size: 1293 1770 521 678 274 147 411 1502
Fragments arranged by size:
1770 1502 1293 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3181 3211 3181
Size: 30 6566
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 190 3229 190
Size: 3039 3557
BseYI C'CCAG_C
Cuts at: 486 497 1829 2021 2050 3107 3254 3332 4021
Size: 11 1332 192 29 1057 147 78 689
Cuts at: 4021 4779 5356 5852 486
Size: 758 577 496 1230
Fragments arranged by size:
1332 1230 1057 758 689 577 496 192
147 78 29 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 328 421 1630 2278 247
Size: 81 93 1209 648 4565
Fragments arranged by size:
4565 1209 648 93 81
BsiEI CG_ry'CG
Cuts at: 104 4138 4184 5392 104
Size: 4034 46 1208 1308
Fragments arranged by size:
4034 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 261 1937 2491 2501 2852 3366 3610 4388 4578
Size: 1676 554 10 351 514 244 778 190
Cuts at: 4578 5370 261
Size: 792 1487
Fragments arranged by size:
1676 1487 792 778 554 514 351 244
190 10
BsmI GAATG_Cn'
Cuts at: 3111 3111
Size: 6596
Bsp1286I G_dGCh'C
Cuts at: 261 493 655 843 1267 1377 1545 1734 1923
Size: 232 162 188 424 110 168 189 189
Cuts at: 1923 1937 2043 2284 2324 2368 2491 2501 2604
Size: 14 106 241 40 44 123 10 103
Cuts at: 2604 2628 2654 2719 2852 3366 3441 3610 4221
Size: 24 26 65 133 514 75 169 611
Cuts at: 4221 4314 4388 4578 4640 5370 261
Size: 93 74 190 62 730 1487
Fragments arranged by size:
1487 730 611 514 424 241 232 190
189 189 188 169 168 162 133 123
110 106 103 93 75 74 65 62
44 40 26 24 14 10
BspEI T'CCGG_A
Cuts at: 722 5816 722
Size: 5094 1502
BspHI T'CATG_A
Cuts at: 5772 5772
Size: 6596
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2351 2518 3646 3860 4162 4543 2351
Size: 167 1128 214 302 381 4404
Fragments arranged by size:
4404 1128 381 302 214 167
BsrBI CCG'CTC
Cuts at: 50 4888 4942 6426 6591 50
Size: 4838 54 1484 165 55
Fragments arranged by size:
4838 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1435 3161 3268 4508 1435
Size: 1726 107 1240 3523
Fragments arranged by size:
3523 1726 1240 107
BsrFI r'CCGG_y
Cuts at: 319 1953 2015 4594 4775 4984 319
Size: 1634 62 2579 181 209 1931
Fragments arranged by size:
2579 1931 1634 209 181 62
BsrGI T'GTAC_A
Cuts at: 3473 3473
Size: 6596
BssSI C'ACGA_G
Cuts at: 247 1150 2162 4867 5225 247
Size: 903 1012 2705 358 1618
Fragments arranged by size:
2705 1618 1012 903 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2684 3973 2684
Size: 1289 5307
BstBI TT'CG_AA
Cuts at: 4957 4957
Size: 6596
BstXI CCAn_nnnn'nTGG
Cuts at: 2043 3420 2043
Size: 1377 5219
BstYI r'GATC_y
Cuts at: 1 238 698 753 860 1100 2988 3621 4088
Size: 237 460 55 107 240 1888 633 467
Cuts at: 4088 4111 4446 4692 5704 5790 5802 6148 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1888 1012 633 467 460 449 346 335
246 240 237 107 86 55 23 12
Bsu36I CC'TnA_GG
Cuts at: 356 356
Size: 6596
BtgI C'CryG_G
Cuts at: 791 1496 1635 1913 2360 2480 2549 791
Size: 705 139 278 447 120 69 4838
Fragments arranged by size:
4838 705 447 278 139 120 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1526 4546 4727 4890 1526
Size: 3020 181 163 3232
Fragments arranged by size:
3232 3020 181 163
BtsI GCAGTG_nn'
Cuts at: 989 1618 1624 2381 2694 2808 3533 3839 989
Size: 629 6 757 313 114 725 306 3746
Fragments arranged by size:
3746 757 725 629 313 306 114 6
ClaI AT'CG_AT
Cuts at: 2958 2958
Size: 6596
DraI TTT'AAA
Cuts at: 3595 3595
Size: 6596
DrdI GACnn_nn'nnGTC
Cuts at: 2062 2443 4302 5160 2062
Size: 381 1859 858 3498
Fragments arranged by size:
3498 1859 858 381
EaeI y'GGCC_r
Cuts at: 278 788 1721 1910 1973 2000 2357 2993 4181
Size: 510 933 189 63 27 357 636 1188
Cuts at: 4181 4355 4746 4773 6118 278
Size: 174 391 27 1345 756
Fragments arranged by size:
1345 1188 933 756 636 510 391 357
189 174 63 27 27
EagI C'GGCC_G
Cuts at: 4181 4181
Size: 6596
EarI CTCTTCn'nnn_
Cuts at: 1204 2021 2383 4619 4829 1204
Size: 817 362 2236 210 2971
Fragments arranged by size:
2971 2236 817 362 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 340 4804 5114 5260 6406 340
Size: 4464 310 146 1146 530
Fragments arranged by size:
4464 1146 530 310 146
EcoICRI GAG'CTC
Cuts at: 2489 2499 2850 3364 3608 2489
Size: 10 351 514 244 5477
Fragments arranged by size:
5477 514 351 244 10
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 715 1155 1253 2263 2515 2866 3186 3253
Size: 429 440 98 1010 252 351 320 67
Cuts at: 3253 3535 3548 4421 4853 5600 5868 286
Size: 282 13 873 432 747 268 1014
Fragments arranged by size:
1014 1010 873 747 440 432 429 351
320 282 268 252 98 67 13
EcoNI CCTnn'n_nnAGG
Cuts at: 1345 1596 1888 1345
Size: 251 292 6053
Fragments arranged by size:
6053 292 251
EcoO109I rG'GnC_Cy
Cuts at: 839 985 1373 1374 1481 1541 1542 1730 1731
Size: 146 388 1 107 60 1 188 1
Cuts at: 1731 1881 1919 2436 2667 3063 3735 839
Size: 150 38 517 231 396 672 3700
Fragments arranged by size:
3700 672 517 396 388 231 188 150
146 107 60 38 1 1 1
EcoRI G'AATT_C
Cuts at: 3600 3600
Size: 6596
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 720 2853 3501 4615 720
Size: 2133 648 1114 2701
Fragments arranged by size:
2701 2133 1114 648
FspI TGC'GCA
Cuts at: 309 4377 309
Size: 4068 2528
HaeII r_GCGC'y
Cuts at: 1027 1867 3457 4030 4278 5300 6279 1027
Size: 840 1590 573 248 1022 979 1344
Fragments arranged by size:
1590 1344 1022 979 840 573 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 230 262 1860 1892 2309 2341 3052 3084 3181
Size: 32 1598 32 417 32 711 32 97
Cuts at: 3181 3213 4438 4468 4470 4500 230
Size: 32 1225 30 2 30 2326
Fragments arranged by size:
2326 1598 1225 711 417 97 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2694 3635 2694
Size: 941 5655
HindIII A'AGCT_T
Cuts at: 3478 3478
Size: 6596
KasI G'GCGC_C
Cuts at: 4026 4274 4026
Size: 248 6348
KpnI G_GTAC'C
Cuts at: 3616 3616
Size: 6596
MluI A'CGCG_T
Cuts at: 6029 6029
Size: 6596
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 789 5267 5451 789
Size: 4478 184 1934
Fragments arranged by size:
4478 1934 184
MscI TGG'CCA
Cuts at: 1723 1975 2002 2995 4357 1723
Size: 252 27 993 1362 3962
Fragments arranged by size:
3962 1362 993 252 27
MslI CAynn'nnrTG
Cuts at: 322 833 1279 1417 1553 2621 3322 3418 4585
Size: 511 446 138 136 1068 701 96 1167
Cuts at: 4585 4712 322
Size: 127 2206
Fragments arranged by size:
2206 1167 1068 701 511 446 138 136
127 96
MspA1I CmG'CkG
Cuts at: 313 342 1089 1815 2684 2844 3185 3563 3707
Size: 29 747 726 869 160 341 378 144
Cuts at: 3707 4021 4381 5394 5639 313
Size: 314 360 1013 245 1270
Fragments arranged by size:
1270 1013 869 747 726 378 360 341
314 245 160 144 29
NaeI GCC'GGC
Cuts at: 4777 4777
Size: 6596
NarI GG'CG_CC
Cuts at: 4027 4275 4027
Size: 248 6348
NgoMIV G'CCGG_C
Cuts at: 4775 4775
Size: 6596
NheI G'CTAG_C
Cuts at: 1335 1335
Size: 6596
NspI r_CATG'y
Cuts at: 327 372 1284 2642 3649 4149 5056 327
Size: 45 912 1358 1007 500 907 1867
Fragments arranged by size:
1867 1358 1007 912 907 500 45
PasI CC'CwG_GG
Cuts at: 1655 2214 2766 1655
Size: 559 552 5485
Fragments arranged by size:
5485 559 552
PciI A'CATG_T
Cuts at: 368 1280 5052 368
Size: 912 3772 1912
Fragments arranged by size:
3772 1912 912
PflMI CCAn_nnn'nTGG
Cuts at: 1430 1430
Size: 6596
PfoI T'CCnGG_A
Cuts at: 191 3209 3339 191
Size: 3018 130 3448
Fragments arranged by size:
3448 3018 130
PmeI GTTT'AAAC
Cuts at: 3595 3595
Size: 6596
PmlI CAC'GTG
Cuts at: 1041 1041
Size: 6596
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 448 480 5735 5767 448
Size: 32 5255 32 1277
Fragments arranged by size:
5255 1277 32 32
PpuMI rG'GwC_Cy
Cuts at: 985 1881 2436 985
Size: 896 555 5145
Fragments arranged by size:
5145 896 555
PshAI GACnn'nnGTC
Cuts at: 2147 2592 2147
Size: 445 6151
PspOMI G'GGCC_C
Cuts at: 839 1373 1541 1730 1919 839
Size: 534 168 189 189 5516
Fragments arranged by size:
5516 534 189 189 168
PstI C_TGCA'G
Cuts at: 1094 1850 2471 2689 3179 3643 4328 1094
Size: 756 621 218 490 464 685 3362
Fragments arranged by size:
3362 756 685 621 490 464 218
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6596
PvuII CAG'CTG
Cuts at: 313 1089 2844 3563 4021 4381 313
Size: 776 1755 719 458 360 2528
Fragments arranged by size:
2528 1755 776 719 458 360
RsrII CG'GwC_CG
Cuts at: 4791 4791
Size: 6596
SacI G_AGCT'C
Cuts at: 2491 2501 2852 3366 3610 2491
Size: 10 351 514 244 5477
Fragments arranged by size:
5477 514 351 244 10
SalI G'TCGA_C
Cuts at: 3633 3633
Size: 6596
SanDI GG'GwC_CC
Cuts at: 985 985
Size: 6596
SapI GCTCTTCn'nnn_
Cuts at: 4619 4829 4619
Size: 210 6386
SbfI CC_TGCA'GG
Cuts at: 3643 3643
Size: 6596
ScaI AGT'ACT
Cuts at: 682 2908 6113 682
Size: 2226 3205 1165
Fragments arranged by size:
3205 2226 1165
SexAI A'CCwGG_T
Cuts at: 1361 1361
Size: 6596
SfcI C'TryA_G
Cuts at: 33 1090 1846 2467 2685 3175 3639 4324 5317
Size: 1057 756 621 218 490 464 685 993
Cuts at: 5317 5508 33
Size: 191 1121
Fragments arranged by size:
1121 1057 993 756 685 621 490 464
218 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1729 1918 1729
Size: 189 6407
SfoI GGC'GCC
Cuts at: 4028 4276 4028
Size: 248 6348
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6596
SmaI CCC'GGG
Cuts at: 1736 1924 3618 1736
Size: 188 1694 4714
Fragments arranged by size:
4714 1694 188
SmlI C'TyrA_G
Cuts at: 3751 4666 5158 5420 3751
Size: 915 492 262 4927
Fragments arranged by size:
4927 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6596
SphI G_CATG'C
Cuts at: 3649 4149 3649
Size: 500 6096
StuI AGG'CCT
Cuts at: 776 2465 776
Size: 1689 4907
StyI C'CwwG_G
Cuts at: 374 1068 1597 2003 3730 4084 374
Size: 694 529 406 1727 354 2886
Fragments arranged by size:
2886 1727 694 529 406 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 730 1429 1903 2431 4179 4312 4771 4980 730
Size: 699 474 528 1748 133 459 209 2346
Fragments arranged by size:
2346 1748 699 528 474 459 209 133
TatI w'GTAC_w
Cuts at: 680 890 2906 3326 3473 6111 680
Size: 210 2016 420 147 2638 1165
Fragments arranged by size:
2638 2016 1165 420 210 147
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1141 1173 2217 2249 2514 2546 6113 6145 1141
Size: 32 1044 32 265 32 3567 32 1592
Fragments arranged by size:
3567 1592 1044 265 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 559 3394 4393 559
Size: 2835 999 2762
Fragments arranged by size:
2835 2762 999
XbaI T'CTAG_A
Cuts at: 3627 3627
Size: 6596
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2841 4091 2841
Size: 1250 5346
XmaI C'CCGG_G
Cuts at: 1734 1922 3616 1734
Size: 188 1694 4714
Fragments arranged by size:
4714 1694 188
ZraI GAC'GTC
Cuts at: 3192 3192
Size: 6596
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AscI BaeI BfrBI BsiWI
BsmBI BssHII BstEII BstZ17I CspCI DraIII EcoRV FseI
FspAI HpaI MfeI NcoI NdeI NotI NruI NsiI
PacI PsiI PspXI PsrI SacII SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI