Restriction map for
pF1KB3343
(Circular) (Six-base) MAPSORT of: pF1KB3343.seq Check: 7453 from: 1 to: 5804
pF1KB3343 5804 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 233 247 2004 233
Size: 14 1757 4033
Fragments arranged by size:
4033 1757 14
AatII G_ACGT'C
Cuts at: 783 783
Size: 5804
AccI GT'mk_AC
Cuts at: 629 2842 629
Size: 2213 3591
Acc65I G'GTAC_C
Cuts at: 2820 2820
Size: 5804
AclI AA'CG_TT
Cuts at: 5644 5644
Size: 5804
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 2758 3629 4061 4808 5076 625
Size: 2133 871 432 747 268 1353
Fragments arranged by size:
2133 1353 871 747 432 268
AflIII A'CryG_T
Cuts at: 3156 4260 5237 3156
Size: 1104 977 3723
Fragments arranged by size:
3723 1104 977
AgeI A'CCGG_T
Cuts at: 4192 4192
Size: 5804
AhdI GACnn_n'nnGTC
Cuts at: 1416 2754 1416
Size: 1338 4466
AleI CACnn'nnGTG
Cuts at: 730 730
Size: 5804
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1318 1318
Size: 5804
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1663 1695 1663
Size: 32 5772
AlwNI CAG_nnn'CTG
Cuts at: 126 204 849 1331 2380 3109 4676 126
Size: 78 645 482 1049 729 1567 1254
Fragments arranged by size:
1567 1254 1049 729 645 482 78
ApaI G_GGCC'C
Cuts at: 1883 2597 1883
Size: 714 5090
ApaLI G'TGCA_C
Cuts at: 4574 4574
Size: 5804
ApoI r'AATT_y
Cuts at: 2808 5157 5413 2808
Size: 2349 256 3199
Fragments arranged by size:
3199 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5804
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5804
AvaI C'yCGr_G
Cuts at: 737 2536 2631 2824 737
Size: 1799 95 193 3717
Fragments arranged by size:
3717 1799 193 95
BamHI G'GATC_C
Cuts at: 2829 2829
Size: 5804
BanI G'GyrC_C
Cuts at: 466 1016 2709 2820 3234 3482 3517 466
Size: 550 1693 111 414 248 35 2753
Fragments arranged by size:
2753 1693 550 414 248 111 35
BanII G_rGCy'C
Cuts at: 515 799 998 1111 1319 1883 1940 2203 2373
Size: 284 199 113 208 564 57 263 170
Cuts at: 2373 2414 2597 2818 3848 515
Size: 41 183 221 1030 2471
Fragments arranged by size:
2471 1030 564 284 263 221 208 199
183 170 113 57 41
BbeI G_GCGC'C
Cuts at: 3238 3486 3238
Size: 248 5556
BbvCI CC'TCA_GC
Cuts at: 985 985
Size: 5804
BciVI GTATCCnnnnn_n'
Cuts at: 3702 4463 5804 3702
Size: 761 1341 3702
Fragments arranged by size:
3702 1341 761
BclI T'GATC_A
Cuts at: 3324 3324
Size: 5804
BglI GCCn_nnn'nGGC
Cuts at: 145 145
Size: 5804
BglII A'GATC_T
Cuts at: 1785 3319 5356 1785
Size: 1534 2037 2233
Fragments arranged by size:
2233 2037 1534
BlpI GC'TnA_GC
Cuts at: 880 2916 880
Size: 2036 3768
Bme1580I G_kGCm'C
Cuts at: 386 469 1040 1883 2597 2705 2714 3429 3522
Size: 83 571 843 714 108 9 715 93
Cuts at: 3522 4578 386
Size: 1056 1612
Fragments arranged by size:
1612 1056 843 715 714 571 108 93
83 9
BmrI ACTGGGnnnn_n'
Cuts at: 973 1341 3016 3097 3432 5576 973
Size: 368 1675 81 335 2144 1201
Fragments arranged by size:
2144 1675 1201 368 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1419 4171 1419
Size: 2752 3052
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 582 634 871 1264 1333 1401 1690 1757
Size: 245 52 237 393 69 68 289 67
Cuts at: 1757 1958 2005 2131 2243 2387 337
Size: 201 47 126 112 144 3754
Fragments arranged by size:
3754 393 289 245 237 201 144 126
112 69 68 67 52 47
Bpu10I CC'TnA_GC
Cuts at: 509 985 1664 509
Size: 476 679 4649
Fragments arranged by size:
4649 679 476
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2980 3859 4351 4649 2980
Size: 879 492 298 4135
Fragments arranged by size:
4135 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 927 1267 5508 927
Size: 340 4241 1223
Fragments arranged by size:
4241 1223 340
BsaAI yAC'GTr
Cuts at: 732 1253 3157 732
Size: 521 1904 3379
Fragments arranged by size:
3379 1904 521
BsaBI GATnn'nnATC
Cuts at: 6 3318 5355 6
Size: 3312 2037 455
Fragments arranged by size:
3312 2037 455
BsaHI Gr'CG_yC
Cuts at: 780 3235 3483 4185 5681 780
Size: 2455 248 702 1496 903
Fragments arranged by size:
2455 1496 903 702 248
BsaWI w'CCGG_w
Cuts at: 2612 2993 3514 4192 4466 4613 5024 2612
Size: 381 521 678 274 147 411 3392
Fragments arranged by size:
3392 678 521 411 381 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 310 340 955 985 1663 1693 1891 1921 1978
Size: 30 615 30 678 30 198 30 57
Cuts at: 1978 2008 310
Size: 30 4106
Fragments arranged by size:
4106 678 615 198 57 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 661 1546 2235 661
Size: 885 689 4230
Fragments arranged by size:
4230 885 689
BseYI C'CCAG_C
Cuts at: 155 266 491 718 784 809 818 836 946
Size: 111 225 227 66 25 9 18 110
Cuts at: 946 1178 1549 1807 1814 2005 2332 3229 3987
Size: 232 371 258 7 191 327 897 758
Cuts at: 3987 4564 5060 155
Size: 577 496 899
Fragments arranged by size:
899 897 758 577 496 371 327 258
232 227 225 191 111 110 66 25
18 9 7
BsiEI CG_ry'CG
Cuts at: 104 3346 3392 4600 104
Size: 3242 46 1208 1308
Fragments arranged by size:
3242 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 710 881 976 1111 1478 2203 2414 2818 3596
Size: 171 95 135 367 725 211 404 778
Cuts at: 3596 3786 4578 710
Size: 190 792 1936
Fragments arranged by size:
1936 792 778 725 404 367 211 190
171 135 95
BsmBI CGTCTCn'nnnn_
Cuts at: 1074 1074
Size: 5804
Bsp1286I G_dGCh'C
Cuts at: 386 469 515 710 799 881 976 998 1040
Size: 83 46 195 89 82 95 22 42
Cuts at: 1040 1111 1319 1478 1883 1940 2203 2373 2414
Size: 71 208 159 405 57 263 170 41
Cuts at: 2414 2597 2705 2714 2818 3429 3522 3596 3786
Size: 183 108 9 104 611 93 74 190
Cuts at: 3786 3848 4578 386
Size: 62 730 1612
Fragments arranged by size:
1612 730 611 405 263 208 195 190
183 170 159 108 104 95 93 89
83 82 74 71 62 57 46 42
41 22 9
BspEI T'CCGG_A
Cuts at: 2612 5024 2612
Size: 2412 3392
BspHI T'CATG_A
Cuts at: 4980 4980
Size: 5804
BspMI ACCTGCnnnn'nnnn_
Cuts at: 233 247 530 2004 2854 3068 3370 3751 233
Size: 14 283 1474 850 214 302 381 2286
Fragments arranged by size:
2286 1474 850 381 302 283 214 14
BsrBI CCG'CTC
Cuts at: 50 1907 4096 4150 5634 5799 50
Size: 1857 2189 54 1484 165 55
Fragments arranged by size:
2189 1857 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3716 3716
Size: 5804
BsrFI r'CCGG_y
Cuts at: 1363 2429 3802 3983 4192 1363
Size: 1066 1373 181 209 2975
Fragments arranged by size:
2975 1373 1066 209 181
BssHII G'CGCG_C
Cuts at: 1399 1399
Size: 5804
BssSI C'ACGA_G
Cuts at: 4075 4433 4075
Size: 358 5446
BstAPI GCAn_nnn'nTGC
Cuts at: 204 714 3181 204
Size: 510 2467 2827
Fragments arranged by size:
2827 2467 510
BstBI TT'CG_AA
Cuts at: 4165 4165
Size: 5804
BstEII G'GTnAC_C
Cuts at: 2441 2441
Size: 5804
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 5804
BstYI r'GATC_y
Cuts at: 1 609 1226 1376 1538 1785 2829 3296 3319
Size: 608 617 150 162 247 1044 467 23
Cuts at: 3319 3654 3900 4912 4998 5010 5356 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1044 1012 617 608 467 449 346 335
247 246 162 150 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2468 2468
Size: 5804
BtgI C'CryG_G
Cuts at: 107 134 1053 1645 2060 107
Size: 27 919 592 415 3851
Fragments arranged by size:
3851 919 592 415 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1452 2056 3754 3935 4098 1452
Size: 604 1698 181 163 3158
Fragments arranged by size:
3158 1698 604 181 163
BtsI GCAGTG_nn'
Cuts at: 1020 3047 1020
Size: 2027 3777
DraI TTT'AAA
Cuts at: 2803 2803
Size: 5804
DrdI GACnn_nn'nnGTC
Cuts at: 3510 4368 3510
Size: 858 4946
EaeI y'GGCC_r
Cuts at: 911 1056 1247 2351 3389 3563 3954 3981 5326
Size: 145 191 1104 1038 174 391 27 1345
Cuts at: 5326 911
Size: 1389
Fragments arranged by size:
1389 1345 1104 1038 391 191 174 145
27
EagI C'GGCC_G
Cuts at: 3389 3389
Size: 5804
EarI CTCTTCn'nnn_
Cuts at: 962 2731 3827 4037 962
Size: 1769 1096 210 2729
Fragments arranged by size:
2729 1769 1096 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4012 4322 4468 5614 4012
Size: 310 146 1146 4202
Fragments arranged by size:
4202 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1109 2201 2412 2816 1109
Size: 1092 211 404 4097
Fragments arranged by size:
4097 1092 404 211
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 582 625 634 871 1264 1333 1401 1690
Size: 245 43 9 237 393 69 68 289
Cuts at: 1690 1757 1958 2005 2131 2243 2387 2758 3629
Size: 67 201 47 126 112 144 371 871
Cuts at: 3629 4061 4808 5076 337
Size: 432 747 268 1065
Fragments arranged by size:
1065 871 747 432 393 371 289 268
245 237 201 144 126 112 69 68
67 47 43 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1323 2662 1323
Size: 1339 4465
EcoO109I rG'GnC_Cy
Cuts at: 309 519 1352 1728 1880 1979 2125 2217 2279
Size: 210 833 376 152 99 146 92 62
Cuts at: 2279 2377 2541 2593 2635 2943 309
Size: 98 164 52 42 308 3170
Fragments arranged by size:
3170 833 376 308 210 164 152 146
99 98 92 62 52 42
EcoRI G'AATT_C
Cuts at: 2808 2808
Size: 5804
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3823 3823
Size: 5804
FspI TGC'GCA
Cuts at: 1519 3585 1519
Size: 2066 3738
HaeII r_GCGC'y
Cuts at: 3238 3486 4508 5487 3238
Size: 248 1022 979 3555
Fragments arranged by size:
3555 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 310 342 424 456 1116 1148 1402 1434 1738
Size: 32 82 32 660 32 254 32 304
Cuts at: 1738 1770 2416 2448 2740 2772 3646 3676 3678
Size: 32 646 32 292 32 874 30 2
Cuts at: 3678 3708 310
Size: 30 2406
Fragments arranged by size:
2406 874 660 646 304 292 254 82
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 279 432 2778 2843 279
Size: 153 2346 65 3240
Fragments arranged by size:
3240 2346 153 65
KasI G'GCGC_C
Cuts at: 3234 3482 3234
Size: 248 5556
KpnI G_GTAC'C
Cuts at: 2824 2824
Size: 5804
MfeI C'AATT_G
Cuts at: 2420 2420
Size: 5804
MluI A'CGCG_T
Cuts at: 5237 5237
Size: 5804
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 471 701 4475 4659 471
Size: 230 3774 184 1616
Fragments arranged by size:
3774 1616 230 184
MscI TGG'CCA
Cuts at: 913 2353 3565 913
Size: 1440 1212 3152
Fragments arranged by size:
3152 1440 1212
MslI CAynn'nnrTG
Cuts at: 730 1123 1471 1742 3793 3920 730
Size: 393 348 271 2051 127 2614
Fragments arranged by size:
2614 2051 393 348 271 127
MspA1I CmG'CkG
Cuts at: 195 270 377 884 1777 2915 3229 3589 4602
Size: 75 107 507 893 1138 314 360 1013
Cuts at: 4602 4847 195
Size: 245 1152
Fragments arranged by size:
1152 1138 1013 893 507 360 314 245
107 75
NaeI GCC'GGC
Cuts at: 3985 3985
Size: 5804
NarI GG'CG_CC
Cuts at: 3235 3483 3235
Size: 248 5556
NcoI C'CATG_G
Cuts at: 107 134 107
Size: 27 5777
NgoMIV G'CCGG_C
Cuts at: 3983 3983
Size: 5804
NspI r_CATG'y
Cuts at: 351 499 1608 1741 2857 3357 4264 351
Size: 148 1109 133 1116 500 907 1891
Fragments arranged by size:
1891 1116 1109 907 500 148 133
PasI CC'CwG_GG
Cuts at: 120 515 604 696 2494 120
Size: 395 89 92 1798 3430
Fragments arranged by size:
3430 1798 395 92 89
PciI A'CATG_T
Cuts at: 4260 4260
Size: 5804
PflMI CCAn_nnn'nTGG
Cuts at: 791 849 1296 1331 1756 2043 791
Size: 58 447 35 425 287 4552
Fragments arranged by size:
4552 447 425 287 58 35
PfoI T'CCnGG_A
Cuts at: 314 314
Size: 5804
PmeI GTTT'AAAC
Cuts at: 2803 2803
Size: 5804
PmlI CAC'GTG
Cuts at: 1253 1253
Size: 5804
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1663 1695 4943 4975 1663
Size: 32 3248 32 2492
Fragments arranged by size:
3248 2492 32 32
PpuMI rG'GwC_Cy
Cuts at: 309 519 1352 1728 1979 2217 2635 309
Size: 210 833 376 251 238 418 3478
Fragments arranged by size:
3478 833 418 376 251 238 210
PspOMI G'GGCC_C
Cuts at: 1879 2593 1879
Size: 714 5090
PstI C_TGCA'G
Cuts at: 200 244 527 2097 2253 2558 2851 3536 200
Size: 44 283 1570 156 305 293 685 2468
Fragments arranged by size:
2468 1570 685 305 293 283 156 44
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5804
PvuII CAG'CTG
Cuts at: 884 1777 3229 3589 884
Size: 893 1452 360 3099
Fragments arranged by size:
3099 1452 893 360
RsrII CG'GwC_CG
Cuts at: 3999 3999
Size: 5804
SacI G_AGCT'C
Cuts at: 1111 2203 2414 2818 1111
Size: 1092 211 404 4097
Fragments arranged by size:
4097 1092 404 211
SalI G'TCGA_C
Cuts at: 2841 2841
Size: 5804
SanDI GG'GwC_CC
Cuts at: 1979 2635 1979
Size: 656 5148
SapI GCTCTTCn'nnn_
Cuts at: 2731 3827 4037 2731
Size: 1096 210 4498
Fragments arranged by size:
4498 1096 210
SbfI CC_TGCA'GG
Cuts at: 244 2851 244
Size: 2607 3197
ScaI AGT'ACT
Cuts at: 5321 5321
Size: 5804
SexAI A'CCwGG_T
Cuts at: 1324 2255 1324
Size: 931 4873
SfcI C'TryA_G
Cuts at: 33 196 240 523 1167 1685 2093 2249 2554
Size: 163 44 283 644 518 408 156 305
Cuts at: 2554 2847 3532 4525 4716 33
Size: 293 685 993 191 1121
Fragments arranged by size:
1121 993 685 644 518 408 305 293
283 191 163 156 44
SfiI GGCCn_nnn'nGGCC
Cuts at: 145 145
Size: 5804
SfoI GGC'GCC
Cuts at: 3236 3484 3236
Size: 248 5556
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5804
SmaI CCC'GGG
Cuts at: 2633 2826 2633
Size: 193 5611
SmlI C'TyrA_G
Cuts at: 2959 3874 4366 4628 2959
Size: 915 492 262 4135
Fragments arranged by size:
4135 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5804
SphI G_CATG'C
Cuts at: 2857 3357 2857
Size: 500 5304
StuI AGG'CCT
Cuts at: 459 459
Size: 5804
StyI C'CwwG_G
Cuts at: 107 134 573 1081 1151 2149 2446 2767 2938
Size: 27 439 508 70 998 297 321 171
Cuts at: 2938 3292 107
Size: 354 2619
Fragments arranged by size:
2619 998 508 439 354 321 297 171
70 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 271 909 1285 1425 3387 3520 3979 4188 271
Size: 638 376 140 1962 133 459 209 1887
Fragments arranged by size:
1962 1887 638 459 376 209 140 133
TatI w'GTAC_w
Cuts at: 1232 5319 1232
Size: 4087 1717
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 583 615 955 987 1368 1400 1891 1923 1978
Size: 32 340 32 381 32 491 32 55
Cuts at: 1978 2010 2045 2077 5321 5353 583
Size: 32 35 32 3244 32 1034
Fragments arranged by size:
3244 1034 491 381 340 55 35 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2639 3601 2639
Size: 962 4842
XbaI T'CTAG_A
Cuts at: 2835 2835
Size: 5804
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 141 806 3299 141
Size: 665 2493 2646
Fragments arranged by size:
2646 2493 665
XmaI C'CCGG_G
Cuts at: 2631 2824 2631
Size: 193 5611
ZraI GAC'GTC
Cuts at: 781 781
Size: 5804
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AscI AvrII BaeI BbsI BcgI BfrBI
BmgBI BmtI BsgI BsiWI BsmI BsrGI BstZ17I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI NdeI
NheI NotI NruI NsiI PacI PshAI PsiI PspXI
PsrI SacII SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI