Restriction map for
pF1KB3349
(Circular) (Six-base) MAPSORT of: pF1KB3349.seq Check: 3576 from: 1 to: 7694
pF1KB3349 7694 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1635 2895 3801 4325 1635
Size: 1260 906 524 5004
Fragments arranged by size:
5004 1260 906 524
AccI GT'mk_AC
Cuts at: 713 3117 4732 713
Size: 2404 1615 3675
Fragments arranged by size:
3675 2404 1615
Acc65I G'GTAC_C
Cuts at: 4710 4710
Size: 7694
AclI AA'CG_TT
Cuts at: 7534 7534
Size: 7694
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 660 908 1027 1630 1660 2206 3535 5519 5951
Size: 248 119 603 30 546 1329 1984 432
Cuts at: 5951 6698 6966 660
Size: 747 268 1388
Fragments arranged by size:
1984 1388 1329 747 603 546 432 268
248 119 30
AfeI AGC'GCT
Cuts at: 741 1363 2263 3487 741
Size: 622 900 1224 4948
Fragments arranged by size:
4948 1224 900 622
AflII C'TTAA_G
Cuts at: 1839 1839
Size: 7694
AflIII A'CryG_T
Cuts at: 1769 4322 5046 6150 7127 1769
Size: 2553 724 1104 977 2336
Fragments arranged by size:
2553 2336 1104 977 724
AgeI A'CCGG_T
Cuts at: 3683 6082 3683
Size: 2399 5295
AhdI GACnn_n'nnGTC
Cuts at: 828 2439 828
Size: 1611 6083
AleI CACnn'nnGTG
Cuts at: 2797 3409 3478 4327 2797
Size: 612 69 849 6164
Fragments arranged by size:
6164 849 612 69
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1621 1621
Size: 7694
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3115 3147 3307 3339 3961 3993 3115
Size: 32 160 32 622 32 6816
Fragments arranged by size:
6816 622 160 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 873 876 934 1052 1074 1200 1680 2025 2277
Size: 3 58 118 22 126 480 345 252
Cuts at: 2277 3089 3249 3574 4999 6566 873
Size: 812 160 325 1425 1567 2001
Fragments arranged by size:
2001 1567 1425 812 480 345 325 252
160 126 118 58 22 3
ApaI G_GGCC'C
Cuts at: 408 2019 2608 408
Size: 1611 589 5494
Fragments arranged by size:
5494 1611 589
ApaLI G'TGCA_C
Cuts at: 169 2161 4045 6464 169
Size: 1992 1884 2419 1399
Fragments arranged by size:
2419 1992 1884 1399
ApoI r'AATT_y
Cuts at: 3164 4615 4698 7047 7303 3164
Size: 1451 83 2349 256 3555
Fragments arranged by size:
3555 2349 1451 256 83
AseI AT'TA_AT
Cuts at: 20 20
Size: 7694
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7694
AvaI C'yCGr_G
Cuts at: 2393 3774 4236 4418 4714 2393
Size: 1381 462 182 296 5373
Fragments arranged by size:
5373 1381 462 296 182
AvrII C'CTAG_G
Cuts at: 1449 1449
Size: 7694
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3623 3656 3623
Size: 33 7661
BamHI G'GATC_C
Cuts at: 1910 2204 4719 1910
Size: 294 2515 4885
Fragments arranged by size:
4885 2515 294
BanI G'GyrC_C
Cuts at: 225 278 973 2002 2684 2879 3577 3915 4710
Size: 53 695 1029 682 195 698 338 795
Cuts at: 4710 5124 5372 5407 225
Size: 414 248 35 2512
Fragments arranged by size:
2512 1029 795 698 695 682 414 338
248 195 53 35
BanII G_rGCy'C
Cuts at: 137 408 1631 1673 2019 2176 2608 2975 3011
Size: 271 1223 42 346 157 432 367 36
Cuts at: 3011 4336 4425 4708 5738 137
Size: 1325 89 283 1030 2093
Fragments arranged by size:
2093 1325 1223 1030 432 367 346 283
271 157 89 42 36
BbeI G_GCGC'C
Cuts at: 3919 5128 5376 3919
Size: 1209 248 6237
Fragments arranged by size:
6237 1209 248
BbsI GAAGACnn'nnnn_
Cuts at: 497 2753 4088 497
Size: 2256 1335 4103
Fragments arranged by size:
4103 2256 1335
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 268 302 268
Size: 34 7660
BciVI GTATCCnnnnn_n'
Cuts at: 5592 6353 7694 5592
Size: 761 1341 5592
Fragments arranged by size:
5592 1341 761
BclI T'GATC_A
Cuts at: 1112 1751 5214 1112
Size: 639 3463 3592
Fragments arranged by size:
3592 3463 639
BglI GCCn_nnn'nGGC
Cuts at: 1018 1807 1018
Size: 789 6905
BglII A'GATC_T
Cuts at: 599 5209 7246 599
Size: 4610 2037 1047
Fragments arranged by size:
4610 2037 1047
BlpI GC'TnA_GC
Cuts at: 1102 3691 4806 1102
Size: 2589 1115 3990
Fragments arranged by size:
3990 2589 1115
Bme1580I G_kGCm'C
Cuts at: 173 230 408 978 2019 2073 2165 2192 2608
Size: 57 178 570 1041 54 92 27 416
Cuts at: 2608 2687 2835 2934 4049 5319 5412 6468 173
Size: 79 148 99 1115 1270 93 1056 1399
Fragments arranged by size:
1399 1270 1115 1056 1041 570 416 178
148 99 93 92 79 57 54 27
BmgBI CAC'GTC
Cuts at: 2484 2484
Size: 7694
BmrI ACTGGGnnnn_n'
Cuts at: 1247 1265 1860 4091 4512 4906 4987 5322 7466
Size: 18 595 2231 421 394 81 335 2144
Cuts at: 7466 1247
Size: 1475
Fragments arranged by size:
2231 2144 1475 595 421 394 335 81
18
BmtI G_CTAG'C
Cuts at: 2299 2581 2299
Size: 282 7412
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 156 4444 6061 156
Size: 4288 1617 1789
Fragments arranged by size:
4288 1789 1617
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 832 898 1017 1490 1657 2299 2326 2710 4147
Size: 66 119 473 167 642 27 384 1437
Cuts at: 4147 4501 4610 832
Size: 354 109 3916
Fragments arranged by size:
3916 1437 642 473 384 354 167 119
109 66 27
Bpu10I CC'TnA_GC
Cuts at: 1924 1924
Size: 7694
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 4870 5749 6241 6539 307
Size: 4563 879 492 298 1462
Fragments arranged by size:
4563 1462 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 727 4022 7398 727
Size: 3295 3376 1023
Fragments arranged by size:
3376 3295 1023
BsaAI yAC'GTr
Cuts at: 1770 1776 3964 5047 1770
Size: 6 2188 1083 4417
Fragments arranged by size:
4417 2188 1083 6
BsaBI GATnn'nnATC
Cuts at: 6 787 5208 7245 6
Size: 781 4421 2037 455
Fragments arranged by size:
4421 2037 781 455
BsaHI Gr'CG_yC
Cuts at: 113 1799 3146 3916 5125 5373 6075 7571 113
Size: 1686 1347 770 1209 248 702 1496 236
Fragments arranged by size:
1686 1496 1347 1209 770 702 248 236
BsaWI w'CCGG_w
Cuts at: 3683 3980 4883 5404 6082 6356 6503 6914 3683
Size: 297 903 521 678 274 147 411 4463
Fragments arranged by size:
4463 903 678 521 411 297 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1063 1093 3059 3089 3307 3337 3385 3415 3661
Size: 30 1966 30 218 30 48 30 246
Cuts at: 3661 3691 3961 3991 1063
Size: 30 270 30 4766
Fragments arranged by size:
4766 1966 270 246 218 48 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 829 2959 3774 4297 829
Size: 2130 815 523 4226
Fragments arranged by size:
4226 2130 815 523
BseYI C'CCAG_C
Cuts at: 969 1165 1358 1630 2130 2192 2613 3141 4337
Size: 196 193 272 500 62 421 528 1196
Cuts at: 4337 5119 5877 6454 6950 969
Size: 782 758 577 496 1713
Fragments arranged by size:
1713 1196 782 758 577 528 500 496
421 272 196 193 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 886 1749 2023 2199 2738 2923 3502 3722 4303
Size: 863 274 176 539 185 579 220 581
Cuts at: 4303 4515 886
Size: 212 4065
Fragments arranged by size:
4065 863 581 579 539 274 220 212
185 176
BsiEI CG_ry'CG
Cuts at: 104 4006 5236 5282 6490 104
Size: 3902 1230 46 1208 1308
Fragments arranged by size:
3902 1308 1230 1208 46
BsiHKAI G_wGCw'C
Cuts at: 137 173 1186 1673 2165 2975 3470 3731 4049
Size: 36 1013 487 492 810 495 261 318
Cuts at: 4049 4336 4366 4708 5486 5676 6468 137
Size: 287 30 342 778 190 792 1363
Fragments arranged by size:
1363 1013 810 792 778 495 492 487
342 318 287 261 190 36 30
BsmBI CGTCTCn'nnnn_
Cuts at: 360 360
Size: 7694
Bsp1286I G_dGCh'C
Cuts at: 137 173 230 408 978 1186 1631 1673 2019
Size: 36 57 178 570 208 445 42 346
Cuts at: 2019 2073 2165 2176 2192 2608 2687 2835 2934
Size: 54 92 11 16 416 79 148 99
Cuts at: 2934 2975 3011 3470 3731 4049 4336 4366 4425
Size: 41 36 459 261 318 287 30 59
Cuts at: 4425 4708 5319 5412 5486 5676 5738 6468 137
Size: 283 611 93 74 190 62 730 1363
Fragments arranged by size:
1363 730 611 570 459 445 416 346
318 287 283 261 208 190 178 148
99 93 92 79 74 62 59 57
54 42 41 36 36 30 16 11
BspEI T'CCGG_A
Cuts at: 3980 6914 3980
Size: 2934 4760
BspHI T'CATG_A
Cuts at: 6870 6870
Size: 7694
BspMI ACCTGCnnnn'nnnn_
Cuts at: 281 1082 1635 2033 2895 3801 4199 4325 4537
Size: 801 553 398 862 906 398 126 212
Cuts at: 4537 4744 4958 5260 5641 281
Size: 207 214 302 381 2334
Fragments arranged by size:
2334 906 862 801 553 398 398 381
302 214 212 207 126
BsrBI CCG'CTC
Cuts at: 50 486 566 3435 3539 5986 6040 7524 7689
Size: 436 80 2869 104 2447 54 1484 165
Cuts at: 7689 50
Size: 55
Fragments arranged by size:
2869 2447 1484 436 165 104 80 55
54
BsrDI GCAATG_nn'
Cuts at: 4637 5606 4637
Size: 969 6725
BsrFI r'CCGG_y
Cuts at: 800 3683 4001 4341 4553 5692 5873 6082 800
Size: 2883 318 340 212 1139 181 209 2412
Fragments arranged by size:
2883 2412 1139 340 318 212 209 181
BssHII G'CGCG_C
Cuts at: 1527 1527
Size: 7694
BssSI C'ACGA_G
Cuts at: 4405 5965 6323 4405
Size: 1560 358 5776
Fragments arranged by size:
5776 1560 358
BstAPI GCAn_nnn'nTGC
Cuts at: 873 3745 5071 873
Size: 2872 1326 3496
Fragments arranged by size:
3496 2872 1326
BstBI TT'CG_AA
Cuts at: 6055 6055
Size: 7694
BstEII G'GTnAC_C
Cuts at: 447 1743 447
Size: 1296 6398
BstXI CCAn_nnnn'nTGG
Cuts at: 289 459 1134 3003 3204 3411 4479 4587 289
Size: 170 675 1869 201 207 1068 108 3396
Fragments arranged by size:
3396 1869 1068 675 207 201 170 108
BstYI r'GATC_y
Cuts at: 1 572 599 850 1586 1910 2204 2769 4719
Size: 571 27 251 736 324 294 565 1950
Cuts at: 4719 5186 5209 5544 5790 6802 6888 6900 7246
Size: 467 23 335 246 1012 86 12 346
Cuts at: 7246 1
Size: 449
Fragments arranged by size:
1950 1012 736 571 565 467 449 346
335 324 294 251 246 86 27 23
12
Bsu36I CC'TnA_GG
Cuts at: 3136 3136
Size: 7694
BtgI C'CryG_G
Cuts at: 107 384 396 1133 2081 2177 2444 3002 3104
Size: 277 12 737 948 96 267 558 102
Cuts at: 3104 3197 3203 107
Size: 93 6 4598
Fragments arranged by size:
4598 948 737 558 277 267 102 96
93 12 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4530 5644 5825 5988 4530
Size: 1114 181 163 6236
Fragments arranged by size:
6236 1114 181 163
BtsI GCAGTG_nn'
Cuts at: 2734 3247 4937 2734
Size: 513 1690 5491
Fragments arranged by size:
5491 1690 513
DraI TTT'AAA
Cuts at: 4693 4693
Size: 7694
DrdI GACnn_nn'nnGTC
Cuts at: 2056 3337 5400 6258 2056
Size: 1281 2063 858 3492
Fragments arranged by size:
3492 2063 1281 858
EaeI y'GGCC_r
Cuts at: 399 461 785 1019 1514 2078 2462 3023 3200
Size: 62 324 234 495 564 384 561 177
Cuts at: 3200 3422 4003 4667 5279 5453 5844 5871 7216
Size: 222 581 664 612 174 391 27 1345
Cuts at: 7216 399
Size: 877
Fragments arranged by size:
1345 877 664 612 581 564 561 495
391 384 324 234 222 177 174 62
27
EagI C'GGCC_G
Cuts at: 4003 5279 4003
Size: 1276 6418
EarI CTCTTCn'nnn_
Cuts at: 836 2225 3442 5717 5927 836
Size: 1389 1217 2275 210 2603
Fragments arranged by size:
2603 2275 1389 1217 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 379 5902 6212 6358 7504 379
Size: 5523 310 146 1146 569
Fragments arranged by size:
5523 1146 569 310 146
EcoICRI GAG'CTC
Cuts at: 135 1671 2973 4334 4706 135
Size: 1536 1302 1361 372 3123
Fragments arranged by size:
3123 1536 1361 1302 372
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 660 832 898 908 1017 1027 1490 1630 1657
Size: 172 66 10 109 10 463 140 27
Cuts at: 1657 1660 2206 2299 2326 2710 3535 4147 4501
Size: 3 546 93 27 384 825 612 354
Cuts at: 4501 4610 5519 5951 6698 6966 660
Size: 109 909 432 747 268 1388
Fragments arranged by size:
1388 909 825 747 612 546 463 432
384 354 268 172 140 109 109 93
66 27 27 10 10 3
EcoNI CCTnn'n_nnAGG
Cuts at: 675 1642 1885 3947 4498 675
Size: 967 243 2062 551 3871
Fragments arranged by size:
3871 2062 967 551 243
EcoO109I rG'GnC_Cy
Cuts at: 405 513 822 986 1011 1071 1453 2022 2058
Size: 108 309 164 25 60 382 569 36
Cuts at: 2058 2089 4221 4241 4584 4833 405
Size: 31 2132 20 343 249 3266
Fragments arranged by size:
3266 2132 569 382 343 309 249 164
108 60 36 31 25 20
EcoRI G'AATT_C
Cuts at: 3164 4698 3164
Size: 1534 6160
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1017 1954 2490 5713 1017
Size: 937 536 3223 2998
Fragments arranged by size:
3223 2998 937 536
FspI TGC'GCA
Cuts at: 1684 4249 5475 1684
Size: 2565 1226 3903
Fragments arranged by size:
3903 2565 1226
HaeII r_GCGC'y
Cuts at: 743 1365 2265 3489 3919 5128 5376 6398 7377
Size: 622 900 1224 430 1209 248 1022 979
Cuts at: 7377 743
Size: 1060
Fragments arranged by size:
1224 1209 1060 1022 979 900 622 430
248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 814 835 846 867 1063 1095 1835 1867 2425
Size: 21 11 21 196 32 740 32 558
Cuts at: 2425 2457 3807 3839 3844 3876 4180 4212 5536
Size: 32 1350 32 5 32 304 32 1324
Cuts at: 5536 5566 5568 5598 814
Size: 30 2 30 2910
Fragments arranged by size:
2910 1350 1324 740 558 304 196 32
32 32 32 32 32 30 30 21
21 11 5 2
HincII GTy'rAC
Cuts at: 714 1338 4733 714
Size: 624 3395 3675
Fragments arranged by size:
3675 3395 624
HindIII A'AGCT_T
Cuts at: 1060 1060
Size: 7694
KasI G'GCGC_C
Cuts at: 3915 5124 5372 3915
Size: 1209 248 6237
Fragments arranged by size:
6237 1209 248
KpnI G_GTAC'C
Cuts at: 4714 4714
Size: 7694
MluI A'CGCG_T
Cuts at: 7127 7127
Size: 7694
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 182 1151 2690 3704 6365 6549 182
Size: 969 1539 1014 2661 184 1327
Fragments arranged by size:
2661 1539 1327 1014 969 184
MscI TGG'CCA
Cuts at: 463 787 1021 2464 3025 3202 3424 4669 5455
Size: 324 234 1443 561 177 222 1245 786
Cuts at: 5455 463
Size: 2702
Fragments arranged by size:
2702 1443 1245 786 561 324 234 222
177
MslI CAynn'nnrTG
Cuts at: 287 781 1231 1348 1603 1723 2572 2797 3202
Size: 494 450 117 255 120 849 225 405
Cuts at: 3202 3370 3409 3478 3700 4327 4567 5683 5810
Size: 168 39 69 222 627 240 1116 127
Cuts at: 5810 287
Size: 2171
Fragments arranged by size:
2171 1116 849 627 494 450 405 255
240 225 222 168 127 120 117 69
39
MspA1I CmG'CkG
Cuts at: 873 1098 1677 2373 2379 2446 2699 2826 3211
Size: 225 579 696 6 67 253 127 385
Cuts at: 3211 4203 4468 4805 5119 5479 6492 6737 873
Size: 992 265 337 314 360 1013 245 1830
Fragments arranged by size:
1830 1013 992 696 579 385 360 337
314 265 253 245 225 127 67 6
NaeI GCC'GGC
Cuts at: 4343 5875 4343
Size: 1532 6162
NarI GG'CG_CC
Cuts at: 3916 5125 5373 3916
Size: 1209 248 6237
Fragments arranged by size:
6237 1209 248
NcoI C'CATG_G
Cuts at: 107 384 396 1133 3104 3197 3203 107
Size: 277 12 737 1971 93 6 4598
Fragments arranged by size:
4598 1971 737 277 93 12 6
NgoMIV G'CCGG_C
Cuts at: 4341 5873 4341
Size: 1532 6162
NheI G'CTAG_C
Cuts at: 2295 2577 2295
Size: 282 7412
NspI r_CATG'y
Cuts at: 1860 4045 4747 5247 6154 1860
Size: 2185 702 500 907 3400
Fragments arranged by size:
3400 2185 907 702 500
PasI CC'CwG_GG
Cuts at: 3920 3920
Size: 7694
PciI A'CATG_T
Cuts at: 6150 6150
Size: 7694
PflMI CCAn_nnn'nTGG
Cuts at: 149 390 861 1953 3077 3686 149
Size: 241 471 1092 1124 609 4157
Fragments arranged by size:
4157 1124 1092 609 471 241
PfoI T'CCnGG_A
Cuts at: 1067 4124 4592 1067
Size: 3057 468 4169
Fragments arranged by size:
4169 3057 468
PmeI GTTT'AAAC
Cuts at: 4693 4693
Size: 7694
PmlI CAC'GTG
Cuts at: 3964 3964
Size: 7694
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3307 3339 3961 3993 6833 6865 3307
Size: 32 622 32 2840 32 4136
Fragments arranged by size:
4136 2840 622 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 513 822 1071 1453 2022 2058 4221 4241 4584
Size: 309 249 382 569 36 2163 20 343
Cuts at: 4584 513
Size: 3623
Fragments arranged by size:
3623 2163 569 382 343 309 249 36
20
PshAI GACnn'nnGTC
Cuts at: 2592 4034 2592
Size: 1442 6252
PspOMI G'GGCC_C
Cuts at: 404 2015 2604 404
Size: 1611 589 5494
Fragments arranged by size:
5494 1611 589
PspXI vC'TCGA_Gb
Cuts at: 2393 2393
Size: 7694
PstI C_TGCA'G
Cuts at: 494 1415 2627 4741 5426 494
Size: 921 1212 2114 685 2762
Fragments arranged by size:
2762 2114 1212 921 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7694
PvuII CAG'CTG
Cuts at: 873 1098 1677 2699 2826 4203 4468 5119 5479
Size: 225 579 1022 127 1377 265 651 360
Cuts at: 5479 873
Size: 3088
Fragments arranged by size:
3088 1377 1022 651 579 360 265 225
127
RsrII CG'GwC_CG
Cuts at: 5889 5889
Size: 7694
SacI G_AGCT'C
Cuts at: 137 1673 2975 4336 4708 137
Size: 1536 1302 1361 372 3123
Fragments arranged by size:
3123 1536 1361 1302 372
SacII CC_GC'GG
Cuts at: 2447 2447
Size: 7694
SalI G'TCGA_C
Cuts at: 712 4731 712
Size: 4019 3675
SanDI GG'GwC_CC
Cuts at: 4241 4241
Size: 7694
SapI GCTCTTCn'nnn_
Cuts at: 3442 5717 5927 3442
Size: 2275 210 5209
Fragments arranged by size:
5209 2275 210
SbfI CC_TGCA'GG
Cuts at: 4741 4741
Size: 7694
ScaI AGT'ACT
Cuts at: 3895 7211 3895
Size: 3316 4378
SexAI A'CCwGG_T
Cuts at: 4016 4016
Size: 7694
SfcI C'TryA_G
Cuts at: 33 490 1275 1411 2623 4737 5422 6415 6606
Size: 457 785 136 1212 2114 685 993 191
Cuts at: 6606 33
Size: 1121
Fragments arranged by size:
2114 1212 1121 993 785 685 457 191
136
SfiI GGCCn_nnn'nGGCC
Cuts at: 1018 1018
Size: 7694
SfoI GGC'GCC
Cuts at: 3917 5126 5374 3917
Size: 1209 248 6237
Fragments arranged by size:
6237 1209 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7694
SmaI CCC'GGG
Cuts at: 3776 4716 3776
Size: 940 6754
SmlI C'TyrA_G
Cuts at: 322 1839 2393 4849 5764 6256 6518 322
Size: 1517 554 2456 915 492 262 1498
Fragments arranged by size:
2456 1517 1498 915 554 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7694
SphI G_CATG'C
Cuts at: 4747 5247 4747
Size: 500 7194
StuI AGG'CCT
Cuts at: 1810 2284 4349 1810
Size: 474 2065 5155
Fragments arranged by size:
5155 2065 474
StyI C'CwwG_G
Cuts at: 107 384 396 434 584 1133 1449 2679 2996
Size: 277 12 38 150 549 316 1230 317
Cuts at: 2996 3104 3197 3203 3326 4828 5182 107
Size: 108 93 6 123 1502 354 2619
Fragments arranged by size:
2619 1502 1230 549 354 317 316 277
150 123 108 93 38 12 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 213 325 961 1255 1752 3912 4531 5277 5410
Size: 112 636 294 497 2160 619 746 133
Cuts at: 5410 5869 6078 213
Size: 459 209 1829
Fragments arranged by size:
2160 1829 746 636 619 497 459 294
209 133 112
TatI w'GTAC_w
Cuts at: 3893 7209 3893
Size: 3316 4378
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 103 135 1251 1283 3059 3091 4279 4311 4345
Size: 32 1116 32 1776 32 1188 32 34
Cuts at: 4345 4377 7211 7243 103
Size: 32 2834 32 554
Fragments arranged by size:
2834 1776 1188 1116 554 34 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2482 2858 5491 2482
Size: 376 2633 4685
Fragments arranged by size:
4685 2633 376
XbaI T'CTAG_A
Cuts at: 4725 4725
Size: 7694
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 324 2964 5189 324
Size: 2640 2225 2829
Fragments arranged by size:
2829 2640 2225
XhoI C'TCGA_G
Cuts at: 2393 2393
Size: 7694
XmaI C'CCGG_G
Cuts at: 3774 4714 3774
Size: 940 6754
XmnI GAAnn'nnTTC
Cuts at: 3319 3319
Size: 7694
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AscI BbvCI BfrBI BsiWI BsmI BsrGI BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI
NdeI NotI NruI NsiI PacI PsiI PsrI SgrAI
SnaBI SrfI SspI SwaI ZraI