Restriction map for
pF1KB3372
(Circular) (Six-base) MAPSORT of: pF1KB3372.seq Check: 7746 from: 1 to: 4448
pF1KB3372 4448 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 298 785 1486 298
Size: 487 701 3260
Fragments arranged by size:
3260 701 487
Acc65I G'GTAC_C
Cuts at: 1464 1464
Size: 4448
AclI AA'CG_TT
Cuts at: 4288 4288
Size: 4448
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 999 2273 2705 3058 3720 280
Size: 719 1274 432 353 662 1008
Fragments arranged by size:
1274 1008 719 662 432 353
AflII C'TTAA_G
Cuts at: 1164 1164
Size: 4448
AflIII A'CryG_T
Cuts at: 554 1800 3600 3881 554
Size: 1246 1800 281 1121
Fragments arranged by size:
1800 1246 1121 281
AhdI GACnn_n'nnGTC
Cuts at: 294 294
Size: 4448
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 707 707
Size: 4448
AlwNI CAG_nnn'CTG
Cuts at: 573 1753 3191 573
Size: 1180 1438 1830
Fragments arranged by size:
1830 1438 1180
ApaLI G'TGCA_C
Cuts at: 3286 3286
Size: 4448
ApoI r'AATT_y
Cuts at: 155 1186 1452 3801 4057 155
Size: 1031 266 2349 256 546
Fragments arranged by size:
2349 1031 546 266 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4448
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4448
AvaI C'yCGr_G
Cuts at: 624 1468 624
Size: 844 3604
BamHI G'GATC_C
Cuts at: 1473 1473
Size: 4448
BanI G'GyrC_C
Cuts at: 340 1252 1464 1878 2126 2161 340
Size: 912 212 414 248 35 2627
Fragments arranged by size:
2627 912 414 248 212 35
BanII G_rGCy'C
Cuts at: 464 1013 1247 1462 2492 464
Size: 549 234 215 1030 2420
Fragments arranged by size:
2420 1030 549 234 215
BbeI G_GCGC'C
Cuts at: 344 1882 2130 344
Size: 1538 248 2662
Fragments arranged by size:
2662 1538 248
BciVI GTATCCnnnnn_n'
Cuts at: 178 968 2346 3402 4448 178
Size: 790 1378 1056 1046 178
Fragments arranged by size:
1378 1056 1046 790 178
BclI T'GATC_A
Cuts at: 1968 1968
Size: 4448
BglI GCCn_nnn'nGGC
Cuts at: 277 665 277
Size: 388 4060
BglII A'GATC_T
Cuts at: 1963 4000 1963
Size: 2037 2411
BlpI GC'TnA_GC
Cuts at: 1560 1560
Size: 4448
Bme1580I G_kGCm'C
Cuts at: 2073 2166 3290 2073
Size: 93 1124 3231
Fragments arranged by size:
3231 1124 93
BmgBI CAC'GTC
Cuts at: 996 996
Size: 4448
BmrI ACTGGGnnnn_n'
Cuts at: 1336 1660 1741 2076 4220 1336
Size: 324 81 335 2144 1564
Fragments arranged by size:
2144 1564 335 324 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 639 2815 639
Size: 2176 2272
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 1120 1277 1352 403
Size: 717 157 75 3499
Fragments arranged by size:
3499 717 157 75
Bpu10I CC'TnA_GC
Cuts at: 948 1025 1241 948
Size: 77 216 4155
Fragments arranged by size:
4155 216 77
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 946 1624 2503 3217 3515 946
Size: 678 879 714 298 1879
Fragments arranged by size:
1879 879 714 678 298
BsaI GGTCTCn'nnnn_
Cuts at: 4152 4152
Size: 4448
BsaAI yAC'GTr
Cuts at: 557 1801 557
Size: 1244 3204
BsaBI GATnn'nnATC
Cuts at: 6 253 1962 3999 6
Size: 247 1709 2037 455
Fragments arranged by size:
2037 1709 455 247
BsaHI Gr'CG_yC
Cuts at: 341 1093 1879 2127 2829 4325 341
Size: 752 786 248 702 1496 464
Fragments arranged by size:
1496 786 752 702 464 248
BsaWI w'CCGG_w
Cuts at: 545 1637 2158 2836 3247 3394 3668 545
Size: 1092 521 678 411 147 274 1325
Fragments arranged by size:
1325 1092 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 808 838 808
Size: 30 4418
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1055 1055
Size: 4448
BseYI C'CCAG_C
Cuts at: 654 1873 2631 3296 3704 654
Size: 1219 758 665 408 1398
Fragments arranged by size:
1398 1219 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 222 796 1044 181
Size: 41 574 248 3585
Fragments arranged by size:
3585 574 248 41
BsiEI CG_ry'CG
Cuts at: 104 231 1990 2036 3266 104
Size: 127 1759 46 1230 1286
Fragments arranged by size:
1759 1286 1230 127 46
BsiHKAI G_wGCw'C
Cuts at: 464 1247 1430 1462 2240 2430 3290 464
Size: 783 183 32 778 190 860 1622
Fragments arranged by size:
1622 860 783 778 190 183 32
BsmI GAATG_Cn'
Cuts at: 901 901
Size: 4448
BsmBI CGTCTCn'nnnn_
Cuts at: 643 828 643
Size: 185 4263
Bsp1286I G_dGCh'C
Cuts at: 464 1013 1247 1430 1462 2073 2166 2240 2430
Size: 549 234 183 32 611 93 74 190
Cuts at: 2430 2492 3290 464
Size: 62 798 1622
Fragments arranged by size:
1622 798 611 549 234 190 183 93
74 62 32
BspHI T'CATG_A
Cuts at: 182 2880 182
Size: 2698 1750
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1498 1712 2014 2395 1498
Size: 214 302 381 3551
Fragments arranged by size:
3551 381 302 214
BsrBI CCG'CTC
Cuts at: 50 631 762 2740 2794 4278 4443 50
Size: 581 131 1978 54 1484 165 55
Fragments arranged by size:
1978 1484 581 165 131 55 54
BsrDI GCAATG_nn'
Cuts at: 2360 2360
Size: 4448
BsrFI r'CCGG_y
Cuts at: 454 2446 2627 454
Size: 1992 181 2275
Fragments arranged by size:
2275 1992 181
BssSI C'ACGA_G
Cuts at: 313 2719 3427 313
Size: 2406 708 1334
Fragments arranged by size:
2406 1334 708
BstAPI GCAn_nnn'nTGC
Cuts at: 801 1825 801
Size: 1024 3424
BstBI TT'CG_AA
Cuts at: 2809 2809
Size: 4448
BstYI r'GATC_y
Cuts at: 1 608 1127 1473 1940 1963 2298 2544 2850
Size: 607 519 346 467 23 335 246 306
Cuts at: 2850 2862 2948 4000 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 607 519 467 449 346 335 306
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 335 497 642 921 1313 107
Size: 228 162 145 279 392 3242
Fragments arranged by size:
3242 392 279 228 162 145
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2398 2579 2742 2398
Size: 181 163 4104
Fragments arranged by size:
4104 181 163
BtsI GCAGTG_nn'
Cuts at: 429 571 831 1070 1691 429
Size: 142 260 239 621 3186
Fragments arranged by size:
3186 621 260 239 142
DraI TTT'AAA
Cuts at: 1447 1447
Size: 4448
DrdI GACnn_nn'nnGTC
Cuts at: 2154 3498 2154
Size: 1344 3104
EaeI y'GGCC_r
Cuts at: 228 269 2033 2207 2598 2625 3970 228
Size: 41 1764 174 391 27 1345 706
Fragments arranged by size:
1764 1345 706 391 174 41 27
EagI C'GGCC_G
Cuts at: 228 2033 228
Size: 1805 2643
EarI CTCTTCn'nnn_
Cuts at: 497 2471 2681 497
Size: 1974 210 2264
Fragments arranged by size:
2264 1974 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 160 1120 1259 2656 3398 3544 4258 160
Size: 960 139 1397 742 146 714 350
Fragments arranged by size:
1397 960 742 714 350 146 139
EcoICRI GAG'CTC
Cuts at: 462 1245 1460 462
Size: 783 215 3450
Fragments arranged by size:
3450 783 215
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 403 999 1120 1277 1352 2273 2705 3058
Size: 123 596 121 157 75 921 432 353
Cuts at: 3058 3720 280
Size: 662 1008
Fragments arranged by size:
1008 921 662 596 432 353 157 123
121 75
EcoNI CCTnn'n_nnAGG
Cuts at: 964 964
Size: 4448
EcoO109I rG'GnC_Cy
Cuts at: 264 378 396 1587 264
Size: 114 18 1191 3125
Fragments arranged by size:
3125 1191 114 18
EcoRI G'AATT_C
Cuts at: 1186 1452 1186
Size: 266 4182
EcoRV GAT'ATC
Cuts at: 1267 1267
Size: 4448
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1206 2467 1206
Size: 1261 3187
FspI TGC'GCA
Cuts at: 2229 2229
Size: 4448
HaeII r_GCGC'y
Cuts at: 220 344 1207 1387 1882 2130 3360 4131 220
Size: 124 863 180 495 248 1230 771 537
Fragments arranged by size:
1230 863 771 537 495 248 180 124
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 280 312 707 739 808 840 1078 1110 2290
Size: 32 395 32 69 32 238 32 1180
Cuts at: 2290 2320 2322 2352 280
Size: 30 2 30 2376
Fragments arranged by size:
2376 1180 395 238 69 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 299 1487 299
Size: 1188 3260
HindIII A'AGCT_T
Cuts at: 1161 1161
Size: 4448
KasI G'GCGC_C
Cuts at: 340 1878 2126 340
Size: 1538 248 2662
Fragments arranged by size:
2662 1538 248
KpnI G_GTAC'C
Cuts at: 1468 1468
Size: 4448
MluI A'CGCG_T
Cuts at: 3881 3881
Size: 4448
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3207 3391 3207
Size: 184 4264
MscI TGG'CCA
Cuts at: 271 2209 271
Size: 1938 2510
MslI CAynn'nnrTG
Cuts at: 1342 2437 2564 1342
Size: 1095 127 3226
Fragments arranged by size:
3226 1095 127
MspA1I CmG'CkG
Cuts at: 511 1117 1559 1873 2233 3017 3262 511
Size: 606 442 314 360 784 245 1697
Fragments arranged by size:
1697 784 606 442 360 314 245
NaeI GCC'GGC
Cuts at: 456 2629 456
Size: 2173 2275
NarI GG'CG_CC
Cuts at: 341 1879 2127 341
Size: 1538 248 2662
Fragments arranged by size:
2662 1538 248
NcoI C'CATG_G
Cuts at: 107 1313 107
Size: 1206 3242
NgoMIV G'CCGG_C
Cuts at: 454 2627 454
Size: 2173 2275
NspI r_CATG'y
Cuts at: 1501 2001 3604 1501
Size: 500 1603 2345
Fragments arranged by size:
2345 1603 500
PasI CC'CwG_GG
Cuts at: 768 768
Size: 4448
PciI A'CATG_T
Cuts at: 3600 3600
Size: 4448
PfoI T'CCnGG_A
Cuts at: 1293 1293
Size: 4448
PmeI GTTT'AAAC
Cuts at: 1447 1447
Size: 4448
PmlI CAC'GTG
Cuts at: 557 557
Size: 4448
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2894 2926 2894
Size: 32 4416
PpuMI rG'GwC_Cy
Cuts at: 264 378 264
Size: 114 4334
PstI C_TGCA'G
Cuts at: 495 797 1311 1495 2180 495
Size: 302 514 184 685 2763
Fragments arranged by size:
2763 685 514 302 184
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4448
PvuII CAG'CTG
Cuts at: 1117 1873 2233 1117
Size: 756 360 3332
Fragments arranged by size:
3332 756 360
RsrII CG'GwC_CG
Cuts at: 2643 2643
Size: 4448
SacI G_AGCT'C
Cuts at: 464 1247 1462 464
Size: 783 215 3450
Fragments arranged by size:
3450 783 215
SalI G'TCGA_C
Cuts at: 297 1485 297
Size: 1188 3260
SapI GCTCTTCn'nnn_
Cuts at: 2471 2681 2471
Size: 210 4238
SbfI CC_TGCA'GG
Cuts at: 797 1495 797
Size: 698 3750
ScaI AGT'ACT
Cuts at: 3965 3965
Size: 4448
SfcI C'TryA_G
Cuts at: 33 491 793 1307 1491 2176 3144 3335 33
Size: 458 302 514 184 685 968 191 1146
Fragments arranged by size:
1146 968 685 514 458 302 191 184
SfiI GGCCn_nnn'nGGCC
Cuts at: 277 277
Size: 4448
SfoI GGC'GCC
Cuts at: 342 1880 2128 342
Size: 1538 248 2662
Fragments arranged by size:
2662 1538 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4448
SmaI CCC'GGG
Cuts at: 1470 1470
Size: 4448
SmlI C'TyrA_G
Cuts at: 961 1164 1603 2518 3232 3494 961
Size: 203 439 915 714 262 1915
Fragments arranged by size:
1915 915 714 439 262 203
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4448
SphI G_CATG'C
Cuts at: 1501 2001 1501
Size: 500 3948
StuI AGG'CCT
Cuts at: 709 709
Size: 4448
StyI C'CwwG_G
Cuts at: 107 704 1207 1313 1582 1936 107
Size: 597 503 106 269 354 2619
Fragments arranged by size:
2619 597 503 354 269 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1273 2031 2164 2623 2832 1273
Size: 758 133 459 209 2889
Fragments arranged by size:
2889 758 459 209 133
TatI w'GTAC_w
Cuts at: 3963 3963
Size: 4448
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 408 440 458 490 3965 3997 408
Size: 32 18 32 3475 32 859
Fragments arranged by size:
3475 859 32 32 32 18
Tth111I GACn'n_nGTC
Cuts at: 820 2245 820
Size: 1425 3023
XbaI T'CTAG_A
Cuts at: 1479 1479
Size: 4448
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 378 1943 114
Size: 264 1565 2619
Fragments arranged by size:
2619 1565 264
XmaI C'CCGG_G
Cuts at: 1468 1468
Size: 4448
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AgeI AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI BsiWI
BspEI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI