Restriction map for
pF1KB3384
(Circular) (Six-base) MAPSORT of: pF1KB3384.seq Check: 5927 from: 1 to: 5210
pF1KB3384 5210 bp
With 182 enzymes: SgfI *
October 14, 2008 16:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 856 856
Size: 5210
AccI GT'mk_AC
Cuts at: 2248 2248
Size: 5210
Acc65I G'GTAC_C
Cuts at: 2226 2226
Size: 5210
AclI AA'CG_TT
Cuts at: 5050 5050
Size: 5210
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 866 2068 3035 3467 4214 4482 866
Size: 1202 967 432 747 268 1594
Fragments arranged by size:
1594 1202 967 747 432 268
AflIII A'CryG_T
Cuts at: 621 1780 2562 3666 4643 621
Size: 1159 782 1104 977 1188
Fragments arranged by size:
1188 1159 1104 977 782
AgeI A'CCGG_T
Cuts at: 3598 3598
Size: 5210
AlwNI CAG_nnn'CTG
Cuts at: 889 1153 1332 2515 4082 889
Size: 264 179 1183 1567 2017
Fragments arranged by size:
2017 1567 1183 264 179
ApaLI G'TGCA_C
Cuts at: 3980 3980
Size: 5210
ApoI r'AATT_y
Cuts at: 1472 2005 2199 2214 4563 4819 1472
Size: 533 194 15 2349 256 1863
Fragments arranged by size:
2349 1863 533 256 194 15
AseI AT'TA_AT
Cuts at: 20 20
Size: 5210
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5210
AvaI C'yCGr_G
Cuts at: 1477 1658 2000 2230 1477
Size: 181 342 230 4457
Fragments arranged by size:
4457 342 230 181
BamHI G'GATC_C
Cuts at: 1519 1689 2235 1519
Size: 170 546 4494
Fragments arranged by size:
4494 546 170
BanI G'GyrC_C
Cuts at: 2226 2640 2888 2923 2226
Size: 414 248 35 4513
Fragments arranged by size:
4513 414 248 35
BanII G_rGCy'C
Cuts at: 115 2224 3254 115
Size: 2109 1030 2071
Fragments arranged by size:
2109 2071 1030
BbeI G_GCGC'C
Cuts at: 2644 2892 2644
Size: 248 4962
BbsI GAAGACnn'nnnn_
Cuts at: 627 1364 1775 1916 1934 627
Size: 737 411 141 18 3903
Fragments arranged by size:
3903 737 411 141 18
BbvCI CC'TCA_GC
Cuts at: 842 1062 1142 1175 2166 842
Size: 220 80 33 991 3886
Fragments arranged by size:
3886 991 220 80 33
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 618 652 618
Size: 34 5176
BciVI GTATCCnnnnn_n'
Cuts at: 611 3108 3869 5210 611
Size: 2497 761 1341 611
Fragments arranged by size:
2497 1341 761 611
BclI T'GATC_A
Cuts at: 2730 2730
Size: 5210
BfrBI ATG'CAT
Cuts at: 820 820
Size: 5210
BglI GCCn_nnn'nGGC
Cuts at: 136 136
Size: 5210
BglII A'GATC_T
Cuts at: 2725 4762 2725
Size: 2037 3173
BlpI GC'TnA_GC
Cuts at: 2322 2322
Size: 5210
Bme1580I G_kGCm'C
Cuts at: 413 486 2835 2928 3984 413
Size: 73 2349 93 1056 1639
Fragments arranged by size:
2349 1639 1056 93 73
BmrI ACTGGGnnnn_n'
Cuts at: 545 691 1643 2422 2503 2838 4982 545
Size: 146 952 779 81 335 2144 773
Fragments arranged by size:
2144 952 779 773 335 146 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 920 3577 920
Size: 2657 2553
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1342 1611 1863 2076 1342
Size: 269 252 213 4476
Fragments arranged by size:
4476 269 252 213
Bpu10I CC'TnA_GC
Cuts at: 842 1062 1142 1175 2166 842
Size: 220 80 33 991 3886
Fragments arranged by size:
3886 991 220 80 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 646 2386 3265 3757 4055 646
Size: 1740 879 492 298 1801
Fragments arranged by size:
1801 1740 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 356 4914 356
Size: 4558 652
BsaAI yAC'GTr
Cuts at: 1268 2563 1268
Size: 1295 3915
BsaBI GATnn'nnATC
Cuts at: 6 1238 2724 4761 6
Size: 1232 1486 2037 455
Fragments arranged by size:
2037 1486 1232 455
BsaHI Gr'CG_yC
Cuts at: 2641 2889 3591 5087 2641
Size: 248 702 1496 2764
Fragments arranged by size:
2764 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2399 2920 3598 3872 4019 4430 2399
Size: 521 678 274 147 411 3179
Fragments arranged by size:
3179 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 203 206 292 295 319 649 863 866 1147
Size: 3 86 3 24 330 214 3 281
Cuts at: 1147 1597 2036 203
Size: 450 439 3377
Fragments arranged by size:
3377 450 439 330 281 214 86 24
3 3 3
BseYI C'CCAG_C
Cuts at: 434 485 2635 3393 3970 4466 434
Size: 51 2150 758 577 496 1178
Fragments arranged by size:
2150 1178 758 577 496 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1052 2093 1052
Size: 1041 4169
BsiEI CG_ry'CG
Cuts at: 104 2752 2798 4006 104
Size: 2648 46 1208 1308
Fragments arranged by size:
2648 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 115 873 1849 2224 3002 3192 3984 115
Size: 758 976 375 778 190 792 1341
Fragments arranged by size:
1341 976 792 778 758 375 190
BsmI GAATG_Cn'
Cuts at: 1704 2182 1704
Size: 478 4732
BsmBI CGTCTCn'nnnn_
Cuts at: 1916 1916
Size: 5210
Bsp1286I G_dGCh'C
Cuts at: 115 413 486 873 1849 2224 2835 2928 3002
Size: 298 73 387 976 375 611 93 74
Cuts at: 3002 3192 3254 3984 115
Size: 190 62 730 1341
Fragments arranged by size:
1341 976 730 611 387 375 298 190
93 74 73 62
BspEI T'CCGG_A
Cuts at: 4430 4430
Size: 5210
BspHI T'CATG_A
Cuts at: 4386 4386
Size: 5210
BspMI ACCTGCnnnn'nnnn_
Cuts at: 856 2260 2474 2776 3157 856
Size: 1404 214 302 381 2909
Fragments arranged by size:
2909 1404 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1793 1816 3502 3556 5040 5205 50
Size: 1743 23 1686 54 1484 165 55
Fragments arranged by size:
1743 1686 1484 165 55 54 23
BsrDI GCAATG_nn'
Cuts at: 470 3122 470
Size: 2652 2558
BsrFI r'CCGG_y
Cuts at: 377 3208 3389 3598 377
Size: 2831 181 209 1989
Fragments arranged by size:
2831 1989 209 181
BssSI C'ACGA_G
Cuts at: 3481 3839 3481
Size: 358 4852
BstAPI GCAn_nnn'nTGC
Cuts at: 1153 2587 1153
Size: 1434 3776
BstBI TT'CG_AA
Cuts at: 638 3571 638
Size: 2933 2277
BstYI r'GATC_y
Cuts at: 1 284 1519 1689 2235 2702 2725 3060 3306
Size: 283 1235 170 546 467 23 335 246
Cuts at: 3306 4318 4404 4416 4762 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1235 1012 546 467 449 346 335 283
246 170 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1373 1373
Size: 5210
BtgI C'CryG_G
Cuts at: 1693 1693
Size: 5210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3160 3341 3504 3160
Size: 181 163 4866
Fragments arranged by size:
4866 181 163
BtsI GCAGTG_nn'
Cuts at: 899 2453 899
Size: 1554 3656
DraI TTT'AAA
Cuts at: 2209 2209
Size: 5210
DraIII CAC_nnn'GTG
Cuts at: 854 1853 854
Size: 999 4211
DrdI GACnn_nn'nnGTC
Cuts at: 574 2916 3774 574
Size: 2342 858 2010
Fragments arranged by size:
2342 2010 858
EaeI y'GGCC_r
Cuts at: 128 375 648 1293 2795 2969 3360 3387 4732
Size: 247 273 645 1502 174 391 27 1345
Cuts at: 4732 128
Size: 606
Fragments arranged by size:
1502 1345 645 606 391 273 247 174
27
EagI C'GGCC_G
Cuts at: 2795 2795
Size: 5210
EarI CTCTTCn'nnn_
Cuts at: 373 706 964 1181 3233 3443 373
Size: 333 258 217 2052 210 2140
Fragments arranged by size:
2140 2052 333 258 217 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3418 3728 3874 5020 3418
Size: 310 146 1146 3608
Fragments arranged by size:
3608 1146 310 146
EcoICRI GAG'CTC
Cuts at: 113 2222 113
Size: 2109 3101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 866 1342 1611 1863 2068 2076 3035 3467 4214
Size: 476 269 252 205 8 959 432 747
Cuts at: 4214 4482 866
Size: 268 1594
Fragments arranged by size:
1594 959 747 476 432 269 268 252
205 8
EcoNI CCTnn'n_nnAGG
Cuts at: 881 881
Size: 5210
EcoO109I rG'GnC_Cy
Cuts at: 315 729 2349 315
Size: 414 1620 3176
Fragments arranged by size:
3176 1620 414
EcoRI G'AATT_C
Cuts at: 2005 2199 2214 2005
Size: 194 15 5001
Fragments arranged by size:
5001 194 15
EcoRV GAT'ATC
Cuts at: 343 343
Size: 5210
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3229 3229
Size: 5210
FspI TGC'GCA
Cuts at: 2991 2991
Size: 5210
HaeII r_GCGC'y
Cuts at: 999 2644 2892 3914 4893 999
Size: 1645 248 1022 979 1316
Fragments arranged by size:
1645 1316 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3052 3082 3084 3114 3052
Size: 30 2 30 5148
Fragments arranged by size:
5148 30 30 2
HincII GTy'rAC
Cuts at: 2249 2249
Size: 5210
HindIII A'AGCT_T
Cuts at: 397 397
Size: 5210
KasI G'GCGC_C
Cuts at: 2640 2888 2640
Size: 248 4962
KpnI G_GTAC'C
Cuts at: 2230 2230
Size: 5210
MluI A'CGCG_T
Cuts at: 1780 4643 1780
Size: 2863 2347
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 704 3881 4065 704
Size: 3177 184 1849
Fragments arranged by size:
3177 1849 184
MscI TGG'CCA
Cuts at: 130 650 1295 2971 130
Size: 520 645 1676 2369
Fragments arranged by size:
2369 1676 645 520
MslI CAynn'nnrTG
Cuts at: 2102 3199 3326 2102
Size: 1097 127 3986
Fragments arranged by size:
3986 1097 127
MspA1I CmG'CkG
Cuts at: 841 1066 1666 1695 2170 2321 2635 2995 4008
Size: 225 600 29 475 151 314 360 1013
Cuts at: 4008 4253 841
Size: 245 1798
Fragments arranged by size:
1798 1013 600 475 360 314 245 225
151 29
NaeI GCC'GGC
Cuts at: 3391 3391
Size: 5210
NarI GG'CG_CC
Cuts at: 2641 2889 2641
Size: 248 4962
NdeI CA'TA_TG
Cuts at: 822 822
Size: 5210
NgoMIV G'CCGG_C
Cuts at: 3389 3389
Size: 5210
NsiI A_TGCA'T
Cuts at: 822 822
Size: 5210
NspI r_CATG'y
Cuts at: 625 2263 2763 3670 625
Size: 1638 500 907 2165
Fragments arranged by size:
2165 1638 907 500
PciI A'CATG_T
Cuts at: 621 3666 621
Size: 3045 2165
PflMI CCAn_nnn'nTGG
Cuts at: 854 1903 854
Size: 1049 4161
PfoI T'CCnGG_A
Cuts at: 115 115
Size: 5210
PmeI GTTT'AAAC
Cuts at: 2209 2209
Size: 5210
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1471 1503 4349 4381 1471
Size: 32 2846 32 2300
Fragments arranged by size:
2846 2300 32 32
PpuMI rG'GwC_Cy
Cuts at: 315 729 315
Size: 414 4796
PstI C_TGCA'G
Cuts at: 894 1004 2257 2942 894
Size: 110 1253 685 3162
Fragments arranged by size:
3162 1253 685 110
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5210
PvuII CAG'CTG
Cuts at: 841 1066 1666 2170 2635 2995 841
Size: 225 600 504 465 360 3056
Fragments arranged by size:
3056 600 504 465 360 225
RsrII CG'GwC_CG
Cuts at: 3405 3405
Size: 5210
SacI G_AGCT'C
Cuts at: 115 2224 115
Size: 2109 3101
SacII CC_GC'GG
Cuts at: 1696 1696
Size: 5210
SalI G'TCGA_C
Cuts at: 2247 2247
Size: 5210
SapI GCTCTTCn'nnn_
Cuts at: 3233 3443 3233
Size: 210 5000
SbfI CC_TGCA'GG
Cuts at: 1004 2257 1004
Size: 1253 3957
ScaI AGT'ACT
Cuts at: 644 1632 4727 644
Size: 988 3095 1127
Fragments arranged by size:
3095 1127 988
SfcI C'TryA_G
Cuts at: 33 890 920 1000 2253 2938 3931 4122 33
Size: 857 30 80 1253 685 993 191 1121
Fragments arranged by size:
1253 1121 993 857 685 191 80 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 136 136
Size: 5210
SfoI GGC'GCC
Cuts at: 2642 2890 2642
Size: 248 4962
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5210
SmaI CCC'GGG
Cuts at: 1660 2232 1660
Size: 572 4638
SmlI C'TyrA_G
Cuts at: 661 2365 3280 3772 4034 661
Size: 1704 915 492 262 1837
Fragments arranged by size:
1837 1704 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5210
SphI G_CATG'C
Cuts at: 2263 2763 2263
Size: 500 4710
SspI AAT'ATT
Cuts at: 1996 1996
Size: 5210
StuI AGG'CCT
Cuts at: 692 692
Size: 5210
StyI C'CwwG_G
Cuts at: 506 1969 2191 2344 2698 506
Size: 1463 222 153 354 3018
Fragments arranged by size:
3018 1463 354 222 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1737 2793 2926 3385 3594 1737
Size: 1056 133 459 209 3353
Fragments arranged by size:
3353 1056 459 209 133
TatI w'GTAC_w
Cuts at: 642 1630 2070 4725 642
Size: 988 440 2655 1127
Fragments arranged by size:
2655 1127 988 440
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4727 4759 4727
Size: 32 5178
Tth111I GACn'n_nGTC
Cuts at: 3007 3007
Size: 5210
XbaI T'CTAG_A
Cuts at: 2241 2241
Size: 5210
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1303 2705 1303
Size: 1402 3808
XmaI C'CCGG_G
Cuts at: 1658 2230 1658
Size: 572 4638
XmnI GAAnn'nnTTC
Cuts at: 1617 1995 2147 2180 1617
Size: 378 152 33 4647
Fragments arranged by size:
4647 378 152 33
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NsiI NspI PciI PflMI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BmgBI BmtI BsaXI BsiWI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI FseI FspAI
HpaI MfeI NcoI NheI NotI NruI PacI PasI
PmlI PshAI PsiI PspOMI PspXI PsrI SanDI SexAI
SgrAI SnaBI SrfI SwaI XhoI ZraI