Restriction map for
pF1KB3391
(Circular) (Six-base) MAPSORT of: pF1KB3391.seq Check: 7964 from: 1 to: 3977
pF1KB3391 3977 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1015 1015
Size: 3977
Acc65I G'GTAC_C
Cuts at: 415 993 415
Size: 578 3399
AclI AA'CG_TT
Cuts at: 3817 3817
Size: 3977
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 482 489 622 1802 2234 2981 3249 482
Size: 7 133 1180 432 747 268 1210
Fragments arranged by size:
1210 1180 747 432 268 133 7
AflIII A'CryG_T
Cuts at: 1329 2433 3410 1329
Size: 1104 977 1896
Fragments arranged by size:
1896 1104 977
AgeI A'CCGG_T
Cuts at: 2365 2365
Size: 3977
AhdI GACnn_n'nnGTC
Cuts at: 643 643
Size: 3977
AlwNI CAG_nnn'CTG
Cuts at: 1282 2849 1282
Size: 1567 2410
ApaLI G'TGCA_C
Cuts at: 2747 2747
Size: 3977
ApoI r'AATT_y
Cuts at: 682 875 947 981 3330 3586 682
Size: 193 72 34 2349 256 1073
Fragments arranged by size:
2349 1073 256 193 72 34
AseI AT'TA_AT
Cuts at: 20 20
Size: 3977
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3977
AvaI C'yCGr_G
Cuts at: 419 544 997 419
Size: 125 453 3399
Fragments arranged by size:
3399 453 125
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 399 407 432 440 399
Size: 8 25 8 3936
Fragments arranged by size:
3936 25 8 8
BamHI G'GATC_C
Cuts at: 1002 1002
Size: 3977
BanI G'GyrC_C
Cuts at: 415 993 1407 1655 1690 415
Size: 578 414 248 35 2702
Fragments arranged by size:
2702 578 414 248 35
BanII G_rGCy'C
Cuts at: 991 2021 991
Size: 1030 2947
BbeI G_GCGC'C
Cuts at: 1411 1659 1411
Size: 248 3729
BbvCI CC'TCA_GC
Cuts at: 902 902
Size: 3977
BciVI GTATCCnnnnn_n'
Cuts at: 1875 2636 3977 1875
Size: 761 1341 1875
Fragments arranged by size:
1875 1341 761
BclI T'GATC_A
Cuts at: 557 852 1497 557
Size: 295 645 3037
Fragments arranged by size:
3037 645 295
BglI GCCn_nnn'nGGC
Cuts at: 581 581
Size: 3977
BglII A'GATC_T
Cuts at: 800 1492 3529 800
Size: 692 2037 1248
Fragments arranged by size:
2037 1248 692
BlpI GC'TnA_GC
Cuts at: 1089 1089
Size: 3977
Bme1580I G_kGCm'C
Cuts at: 1602 1695 2751 1602
Size: 93 1056 2828
Fragments arranged by size:
2828 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1189 1270 1605 3749 1189
Size: 81 335 2144 1417
Fragments arranged by size:
2144 1417 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2344 2344
Size: 3977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 892 892
Size: 3977
Bpu10I CC'TnA_GC
Cuts at: 902 902
Size: 3977
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1153 2032 2524 2822 1153
Size: 879 492 298 2308
Fragments arranged by size:
2308 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3681 3681
Size: 3977
BsaAI yAC'GTr
Cuts at: 516 1330 516
Size: 814 3163
BsaBI GATnn'nnATC
Cuts at: 6 1491 3528 6
Size: 1485 2037 455
Fragments arranged by size:
2037 1485 455
BsaHI Gr'CG_yC
Cuts at: 573 1408 1656 2358 3854 573
Size: 835 248 702 1496 696
Fragments arranged by size:
1496 835 702 696 248
BsaWI w'CCGG_w
Cuts at: 1166 1687 2365 2639 2786 3197 1166
Size: 521 678 274 147 411 1946
Fragments arranged by size:
1946 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1402 2160 2737 3233 1402
Size: 758 577 496 2146
Fragments arranged by size:
2146 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1519 1565 2773 104
Size: 1415 46 1208 1308
Fragments arranged by size:
1415 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 991 1769 1959 2751 991
Size: 778 190 792 2217
Fragments arranged by size:
2217 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 991 1602 1695 1769 1959 2021 2751 991
Size: 611 93 74 190 62 730 2217
Fragments arranged by size:
2217 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3197 3197
Size: 3977
BspHI T'CATG_A
Cuts at: 3153 3153
Size: 3977
BspMI ACCTGCnnnn'nnnn_
Cuts at: 263 926 1027 1241 1543 1924 263
Size: 663 101 214 302 381 2316
Fragments arranged by size:
2316 663 381 302 214 101
BsrBI CCG'CTC
Cuts at: 50 2269 2323 3807 3972 50
Size: 2219 54 1484 165 55
Fragments arranged by size:
2219 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 234 689 726 1889 234
Size: 455 37 1163 2322
Fragments arranged by size:
2322 1163 455 37
BsrFI r'CCGG_y
Cuts at: 1975 2156 2365 1975
Size: 181 209 3587
Fragments arranged by size:
3587 209 181
BsrGI T'GTAC_A
Cuts at: 311 311
Size: 3977
BssSI C'ACGA_G
Cuts at: 2248 2606 2248
Size: 358 3619
BstAPI GCAn_nnn'nTGC
Cuts at: 1354 1354
Size: 3977
BstBI TT'CG_AA
Cuts at: 2338 2338
Size: 3977
BstEII G'GTnAC_C
Cuts at: 196 196
Size: 3977
BstYI r'GATC_y
Cuts at: 1 800 1002 1469 1492 1827 2073 3085 3171
Size: 799 202 467 23 335 246 1012 86
Cuts at: 3171 3183 3529 1
Size: 12 346 449
Fragments arranged by size:
1012 799 467 449 346 335 246 202
86 23 12
BtgI C'CryG_G
Cuts at: 107 489 107
Size: 382 3595
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1927 2108 2271 1927
Size: 181 163 3633
Fragments arranged by size:
3633 181 163
BtsI GCAGTG_nn'
Cuts at: 1220 1220
Size: 3977
ClaI AT'CG_AT
Cuts at: 710 710
Size: 3977
DraI TTT'AAA
Cuts at: 976 976
Size: 3977
DrdI GACnn_nn'nnGTC
Cuts at: 1683 2541 1683
Size: 858 3119
EaeI y'GGCC_r
Cuts at: 1562 1736 2127 2154 3499 1562
Size: 174 391 27 1345 2040
Fragments arranged by size:
2040 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1562 1562
Size: 3977
EarI CTCTTCn'nnn_
Cuts at: 2000 2210 2000
Size: 210 3767
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2185 2495 2641 3787 2185
Size: 310 146 1146 2375
Fragments arranged by size:
2375 1146 310 146
EcoICRI GAG'CTC
Cuts at: 989 989
Size: 3977
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 482 489 622 892 1802 2234 2981 3249 482
Size: 7 133 270 910 432 747 268 1210
Fragments arranged by size:
1210 910 747 432 270 268 133 7
EcoO109I rG'GnC_Cy
Cuts at: 1116 1116
Size: 3977
EcoRI G'AATT_C
Cuts at: 981 981
Size: 3977
EcoRV GAT'ATC
Cuts at: 714 714
Size: 3977
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1996 1996
Size: 3977
FspI TGC'GCA
Cuts at: 1758 1758
Size: 3977
HaeII r_GCGC'y
Cuts at: 1411 1659 2681 3660 1411
Size: 248 1022 979 1728
Fragments arranged by size:
1728 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 624 629 656 661 1819 1849 1851 1881 624
Size: 5 27 5 1158 30 2 30 2720
Fragments arranged by size:
2720 1158 30 30 27 5 5 2
HincII GTy'rAC
Cuts at: 1016 1016
Size: 3977
KasI G'GCGC_C
Cuts at: 1407 1655 1407
Size: 248 3729
KpnI G_GTAC'C
Cuts at: 419 997 419
Size: 578 3399
MluI A'CGCG_T
Cuts at: 3410 3410
Size: 3977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2648 2832 2648
Size: 184 3793
MscI TGG'CCA
Cuts at: 1738 1738
Size: 3977
MslI CAynn'nnrTG
Cuts at: 1966 2093 1966
Size: 127 3850
MspA1I CmG'CkG
Cuts at: 609 1088 1402 1762 2775 3020 609
Size: 479 314 360 1013 245 1566
Fragments arranged by size:
1566 1013 479 360 314 245
NaeI GCC'GGC
Cuts at: 2158 2158
Size: 3977
NarI GG'CG_CC
Cuts at: 1408 1656 1408
Size: 248 3729
NcoI C'CATG_G
Cuts at: 107 107
Size: 3977
NgoMIV G'CCGG_C
Cuts at: 2156 2156
Size: 3977
NspI r_CATG'y
Cuts at: 1030 1530 2437 1030
Size: 500 907 2570
Fragments arranged by size:
2570 907 500
PciI A'CATG_T
Cuts at: 2433 2433
Size: 3977
PflMI CCAn_nnn'nTGG
Cuts at: 397 397
Size: 3977
PmeI GTTT'AAAC
Cuts at: 976 976
Size: 3977
PmlI CAC'GTG
Cuts at: 516 516
Size: 3977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3116 3148 3116
Size: 32 3945
PstI C_TGCA'G
Cuts at: 1024 1709 1024
Size: 685 3292
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3977
PvuII CAG'CTG
Cuts at: 609 1402 1762 609
Size: 793 360 2824
Fragments arranged by size:
2824 793 360
RsrII CG'GwC_CG
Cuts at: 2172 2172
Size: 3977
SacI G_AGCT'C
Cuts at: 991 991
Size: 3977
SalI G'TCGA_C
Cuts at: 1014 1014
Size: 3977
SapI GCTCTTCn'nnn_
Cuts at: 2000 2210 2000
Size: 210 3767
SbfI CC_TGCA'GG
Cuts at: 1024 1024
Size: 3977
ScaI AGT'ACT
Cuts at: 325 3494 325
Size: 3169 808
SfcI C'TryA_G
Cuts at: 33 1020 1705 2698 2889 33
Size: 987 685 993 191 1121
Fragments arranged by size:
1121 993 987 685 191
SfoI GGC'GCC
Cuts at: 1409 1657 1409
Size: 248 3729
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3977
SmaI CCC'GGG
Cuts at: 421 546 999 421
Size: 125 453 3399
Fragments arranged by size:
3399 453 125
SmlI C'TyrA_G
Cuts at: 1132 2047 2539 2801 1132
Size: 915 492 262 2308
Fragments arranged by size:
2308 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3977
SphI G_CATG'C
Cuts at: 1030 1530 1030
Size: 500 3477
StyI C'CwwG_G
Cuts at: 107 341 1111 1465 107
Size: 234 770 354 2619
Fragments arranged by size:
2619 770 354 234
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1560 1693 2152 2361 1560
Size: 133 459 209 3176
Fragments arranged by size:
3176 459 209 133
TatI w'GTAC_w
Cuts at: 311 323 3492 311
Size: 12 3169 796
Fragments arranged by size:
3169 796 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3494 3526 3494
Size: 32 3945
Tth111I GACn'n_nGTC
Cuts at: 1774 1774
Size: 3977
XbaI T'CTAG_A
Cuts at: 1008 1008
Size: 3977
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1472 1472
Size: 3977
XmaI C'CCGG_G
Cuts at: 419 544 997 419
Size: 125 453 3399
Fragments arranged by size:
3399 453 125
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PmlI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BbsI BcgI BfrBI BmgBI BmtI BsaXI
BseRI BsgI BsiWI BsmI BsmBI BssHII BstXI BstZ17I
Bsu36I CspCI DraIII EcoNI FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI