Restriction map for
pF1KB3392
(Circular) (Six-base) MAPSORT of: pF1KB3392.seq Check: 4597 from: 1 to: 4151
pF1KB3392 4151 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 572 1189 572
Size: 617 3534
Acc65I G'GTAC_C
Cuts at: 1167 1167
Size: 4151
AclI AA'CG_TT
Cuts at: 1145 3991 1145
Size: 2846 1305
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 607 1976 2408 3155 3423 415
Size: 192 1369 432 747 268 1143
Fragments arranged by size:
1369 1143 747 432 268 192
AflIII A'CryG_T
Cuts at: 764 1503 2607 3584 764
Size: 739 1104 977 1331
Fragments arranged by size:
1331 1104 977 739
AgeI A'CCGG_T
Cuts at: 2539 2539
Size: 4151
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 479 479
Size: 4151
AlwNI CAG_nnn'CTG
Cuts at: 1456 3023 1456
Size: 1567 2584
ApaLI G'TGCA_C
Cuts at: 2921 2921
Size: 4151
ApoI r'AATT_y
Cuts at: 124 1155 3504 3760 124
Size: 1031 2349 256 515
Fragments arranged by size:
2349 1031 515 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4151
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
AvaI C'yCGr_G
Cuts at: 1171 1171
Size: 4151
BamHI G'GATC_C
Cuts at: 1176 1176
Size: 4151
BanI G'GyrC_C
Cuts at: 1167 1581 1829 1864 1167
Size: 414 248 35 3454
Fragments arranged by size:
3454 414 248 35
BanII G_rGCy'C
Cuts at: 220 1165 2195 220
Size: 945 1030 2176
Fragments arranged by size:
2176 1030 945
BbeI G_GCGC'C
Cuts at: 1585 1833 1585
Size: 248 3903
BbsI GAAGACnn'nnnn_
Cuts at: 701 701
Size: 4151
BciVI GTATCCnnnnn_n'
Cuts at: 2049 2810 4151 2049
Size: 761 1341 2049
Fragments arranged by size:
2049 1341 761
BclI T'GATC_A
Cuts at: 995 1671 995
Size: 676 3475
BglII A'GATC_T
Cuts at: 594 1666 3703 594
Size: 1072 2037 1042
Fragments arranged by size:
2037 1072 1042
BlpI GC'TnA_GC
Cuts at: 1263 1263
Size: 4151
Bme1580I G_kGCm'C
Cuts at: 1776 1869 2925 1776
Size: 93 1056 3002
Fragments arranged by size:
3002 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1363 1444 1779 3923 1363
Size: 81 335 2144 1591
Fragments arranged by size:
2144 1591 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2518 2518
Size: 4151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 434 647 889 346
Size: 88 213 242 3608
Fragments arranged by size:
3608 242 213 88
Bpu10I CC'TnA_GC
Cuts at: 659 659
Size: 4151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 115 439 1327 2206 2698 2996 115
Size: 324 888 879 492 298 1270
Fragments arranged by size:
1270 888 879 492 324 298
BsaI GGTCTCn'nnnn_
Cuts at: 3855 3855
Size: 4151
BsaAI yAC'GTr
Cuts at: 1504 1504
Size: 4151
BsaBI GATnn'nnATC
Cuts at: 6 1665 3702 6
Size: 1659 2037 455
Fragments arranged by size:
2037 1659 455
BsaHI Gr'CG_yC
Cuts at: 1582 1830 2532 4028 1582
Size: 248 702 1496 1705
Fragments arranged by size:
1705 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1340 1861 2539 2813 2960 3371 1340
Size: 521 678 274 147 411 2120
Fragments arranged by size:
2120 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1130 1160 1130
Size: 30 4121
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 436 436
Size: 4151
BseYI C'CCAG_C
Cuts at: 1576 2334 2911 3407 1576
Size: 758 577 496 2320
Fragments arranged by size:
2320 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1693 1739 2947 104
Size: 1589 46 1208 1308
Fragments arranged by size:
1589 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1165 1943 2133 2925 1165
Size: 778 190 792 2391
Fragments arranged by size:
2391 792 778 190
BsmI GAATG_Cn'
Cuts at: 1045 1045
Size: 4151
Bsp1286I G_dGCh'C
Cuts at: 220 1165 1776 1869 1943 2133 2195 2925 220
Size: 945 611 93 74 190 62 730 1446
Fragments arranged by size:
1446 945 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3371 3371
Size: 4151
BspHI T'CATG_A
Cuts at: 3327 3327
Size: 4151
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1201 1415 1717 2098 1201
Size: 214 302 381 3254
Fragments arranged by size:
3254 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2443 2497 3981 4146 50
Size: 2393 54 1484 165 55
Fragments arranged by size:
2393 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 119 2063 119
Size: 1944 2207
BsrFI r'CCGG_y
Cuts at: 2149 2330 2539 2149
Size: 181 209 3761
Fragments arranged by size:
3761 209 181
BssSI C'ACGA_G
Cuts at: 2422 2780 2422
Size: 358 3793
BstAPI GCAn_nnn'nTGC
Cuts at: 1528 1528
Size: 4151
BstBI TT'CG_AA
Cuts at: 2512 2512
Size: 4151
BstEII G'GTnAC_C
Cuts at: 412 664 412
Size: 252 3899
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4151
BstYI r'GATC_y
Cuts at: 1 594 1176 1643 1666 2001 2247 3259 3345
Size: 593 582 467 23 335 246 1012 86
Cuts at: 3345 3357 3703 1
Size: 12 346 449
Fragments arranged by size:
1012 593 582 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4151
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2101 2282 2445 2101
Size: 181 163 3807
Fragments arranged by size:
3807 181 163
BtsI GCAGTG_nn'
Cuts at: 1394 1394
Size: 4151
DraI TTT'AAA
Cuts at: 1150 1150
Size: 4151
DrdI GACnn_nn'nnGTC
Cuts at: 1857 2715 1857
Size: 858 3293
EaeI y'GGCC_r
Cuts at: 599 1736 1910 2301 2328 3673 599
Size: 1137 174 391 27 1345 1077
Fragments arranged by size:
1345 1137 1077 391 174 27
EagI C'GGCC_G
Cuts at: 1736 1736
Size: 4151
EarI CTCTTCn'nnn_
Cuts at: 152 583 2174 2384 152
Size: 431 1591 210 1919
Fragments arranged by size:
1919 1591 431 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2359 2669 2815 3961 2359
Size: 310 146 1146 2549
Fragments arranged by size:
2549 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1163 1163
Size: 4151
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 415 434 607 647 889 1976 2408 3155
Size: 69 19 173 40 242 1087 432 747
Cuts at: 3155 3423 346
Size: 268 1074
Fragments arranged by size:
1087 1074 747 432 268 242 173 69
40 19
EcoNI CCTnn'n_nnAGG
Cuts at: 133 421 133
Size: 288 3863
EcoO109I rG'GnC_Cy
Cuts at: 492 719 1290 492
Size: 227 571 3353
Fragments arranged by size:
3353 571 227
EcoRI G'AATT_C
Cuts at: 124 1155 124
Size: 1031 3120
EcoRV GAT'ATC
Cuts at: 1038 1038
Size: 4151
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2170 2170
Size: 4151
FspI TGC'GCA
Cuts at: 1932 1932
Size: 4151
HaeII r_GCGC'y
Cuts at: 1585 1833 2855 3834 1585
Size: 248 1022 979 1902
Fragments arranged by size:
1902 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 207 239 598 630 1993 2023 2025 2055 207
Size: 32 359 32 1363 30 2 30 2303
Fragments arranged by size:
2303 1363 359 32 32 30 30 2
HincII GTy'rAC
Cuts at: 340 1190 340
Size: 850 3301
HindIII A'AGCT_T
Cuts at: 949 949
Size: 4151
KasI G'GCGC_C
Cuts at: 1581 1829 1581
Size: 248 3903
KpnI G_GTAC'C
Cuts at: 1171 1171
Size: 4151
MluI A'CGCG_T
Cuts at: 3584 3584
Size: 4151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 954 2822 3006 954
Size: 1868 184 2099
Fragments arranged by size:
2099 1868 184
MscI TGG'CCA
Cuts at: 601 1912 601
Size: 1311 2840
MslI CAynn'nnrTG
Cuts at: 112 2140 2267 112
Size: 2028 127 1996
Fragments arranged by size:
2028 1996 127
MspA1I CmG'CkG
Cuts at: 211 1262 1576 1936 2949 3194 211
Size: 1051 314 360 1013 245 1168
Fragments arranged by size:
1168 1051 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2332 2332
Size: 4151
NarI GG'CG_CC
Cuts at: 1582 1830 1582
Size: 248 3903
NcoI C'CATG_G
Cuts at: 107 107
Size: 4151
NgoMIV G'CCGG_C
Cuts at: 2330 2330
Size: 4151
NspI r_CATG'y
Cuts at: 1204 1704 2611 1204
Size: 500 907 2744
Fragments arranged by size:
2744 907 500
PciI A'CATG_T
Cuts at: 2607 2607
Size: 4151
PfoI T'CCnGG_A
Cuts at: 201 362 1088 201
Size: 161 726 3264
Fragments arranged by size:
3264 726 161
PmeI GTTT'AAAC
Cuts at: 1150 1150
Size: 4151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3290 3322 3290
Size: 32 4119
PstI C_TGCA'G
Cuts at: 1198 1883 1198
Size: 685 3466
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4151
PvuII CAG'CTG
Cuts at: 1576 1936 1576
Size: 360 3791
RsrII CG'GwC_CG
Cuts at: 2346 2346
Size: 4151
SacI G_AGCT'C
Cuts at: 1165 1165
Size: 4151
SalI G'TCGA_C
Cuts at: 1188 1188
Size: 4151
SapI GCTCTTCn'nnn_
Cuts at: 152 2174 2384 152
Size: 2022 210 1919
Fragments arranged by size:
2022 1919 210
SbfI CC_TGCA'GG
Cuts at: 1198 1198
Size: 4151
ScaI AGT'ACT
Cuts at: 3668 3668
Size: 4151
SfcI C'TryA_G
Cuts at: 33 573 1194 1879 2872 3063 33
Size: 540 621 685 993 191 1121
Fragments arranged by size:
1121 993 685 621 540 191
SfoI GGC'GCC
Cuts at: 1583 1831 1583
Size: 248 3903
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
SmaI CCC'GGG
Cuts at: 1173 1173
Size: 4151
SmlI C'TyrA_G
Cuts at: 130 418 1306 2221 2713 2975 130
Size: 288 888 915 492 262 1306
Fragments arranged by size:
1306 915 888 492 288 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4151
SphI G_CATG'C
Cuts at: 1204 1704 1204
Size: 500 3651
StuI AGG'CCT
Cuts at: 139 139
Size: 4151
StyI C'CwwG_G
Cuts at: 107 495 1285 1639 107
Size: 388 790 354 2619
Fragments arranged by size:
2619 790 388 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 683 1734 1867 2326 2535 683
Size: 1051 133 459 209 2299
Fragments arranged by size:
2299 1051 459 209 133
TatI w'GTAC_w
Cuts at: 833 3666 833
Size: 2833 1318
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 197 229 3668 3700 197
Size: 32 3439 32 648
Fragments arranged by size:
3439 648 32 32
Tth111I GACn'n_nGTC
Cuts at: 1948 1948
Size: 4151
XbaI T'CTAG_A
Cuts at: 1182 1182
Size: 4151
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1646 1646
Size: 4151
XmaI C'CCGG_G
Cuts at: 1171 1171
Size: 4151
XmnI GAAnn'nnTTC
Cuts at: 170 170
Size: 4151
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BsgI BsiWI BsmBI BsrGI BssHII BstZ17I Bsu36I
ClaI CspCI DraIII FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI