Restriction map for
pF1KB3400
(Circular) (Six-base) MAPSORT of: pF1KB3400.seq Check: 6334 from: 1 to: 3428
pF1KB3400 3428 bp
With 182 enzymes: SgfI *
January 9, 2009 11:10 ..
AccI GT'mk_AC
Cuts at: 466 466
Size: 3428
Acc65I G'GTAC_C
Cuts at: 444 444
Size: 3428
AclI AA'CG_TT
Cuts at: 3268 3268
Size: 3428
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1253 1685 2038 2700 1253
Size: 432 353 662 1981
Fragments arranged by size:
1981 662 432 353
AflIII A'CryG_T
Cuts at: 780 2580 2861 780
Size: 1800 281 1347
Fragments arranged by size:
1800 1347 281
AlwNI CAG_nnn'CTG
Cuts at: 733 2171 733
Size: 1438 1990
ApaLI G'TGCA_C
Cuts at: 2266 2266
Size: 3428
ApoI r'AATT_y
Cuts at: 432 2781 3037 432
Size: 2349 256 823
Fragments arranged by size:
2349 823 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3428
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3428
AvaI C'yCGr_G
Cuts at: 448 448
Size: 3428
BamHI G'GATC_C
Cuts at: 453 453
Size: 3428
BanI G'GyrC_C
Cuts at: 444 858 1106 1141 444
Size: 414 248 35 2731
Fragments arranged by size:
2731 414 248 35
BanII G_rGCy'C
Cuts at: 442 1472 442
Size: 1030 2398
BbeI G_GCGC'C
Cuts at: 862 1110 862
Size: 248 3180
BbsI GAAGACnn'nnnn_
Cuts at: 232 232
Size: 3428
BciVI GTATCCnnnnn_n'
Cuts at: 238 288 1326 2382 3428 238
Size: 50 1038 1056 1046 238
Fragments arranged by size:
1056 1046 1038 238 50
BclI T'GATC_A
Cuts at: 948 948
Size: 3428
BglII A'GATC_T
Cuts at: 943 2980 943
Size: 2037 1391
BlpI GC'TnA_GC
Cuts at: 540 540
Size: 3428
Bme1580I G_kGCm'C
Cuts at: 1053 1146 2270 1053
Size: 93 1124 2211
Fragments arranged by size:
2211 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 150 203 640 721 1056 3200 150
Size: 53 437 81 335 2144 378
Fragments arranged by size:
2144 437 378 335 81 53
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1795 1795
Size: 3428
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 1483 2197 2495 604
Size: 879 714 298 1537
Fragments arranged by size:
1537 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 217 3132 217
Size: 2915 513
BsaAI yAC'GTr
Cuts at: 781 781
Size: 3428
BsaBI GATnn'nnATC
Cuts at: 6 942 2979 6
Size: 936 2037 455
Fragments arranged by size:
2037 936 455
BsaHI Gr'CG_yC
Cuts at: 859 1107 1809 3305 859
Size: 248 702 1496 982
Fragments arranged by size:
1496 982 702 248
BsaWI w'CCGG_w
Cuts at: 617 1138 1816 2227 2374 2648 617
Size: 521 678 411 147 274 1397
Fragments arranged by size:
1397 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 853 1611 2276 2684 853
Size: 758 665 408 1597
Fragments arranged by size:
1597 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 970 1016 2246 104
Size: 866 46 1230 1286
Fragments arranged by size:
1286 1230 866 46
BsiHKAI G_wGCw'C
Cuts at: 442 1220 1410 2270 442
Size: 778 190 860 1600
Fragments arranged by size:
1600 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 442 1053 1146 1220 1410 1472 2270 442
Size: 611 93 74 190 62 798 1600
Fragments arranged by size:
1600 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 1860 1860
Size: 3428
BspMI ACCTGCnnnn'nnnn_
Cuts at: 478 692 994 1375 478
Size: 214 302 381 2531
Fragments arranged by size:
2531 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1720 1774 3258 3423 50
Size: 1670 54 1484 165 55
Fragments arranged by size:
1670 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1340 1340
Size: 3428
BsrFI r'CCGG_y
Cuts at: 1426 1607 1426
Size: 181 3247
BssSI C'ACGA_G
Cuts at: 1699 2407 1699
Size: 708 2720
BstAPI GCAn_nnn'nTGC
Cuts at: 805 805
Size: 3428
BstBI TT'CG_AA
Cuts at: 1789 1789
Size: 3428
BstYI r'GATC_y
Cuts at: 1 453 920 943 1278 1524 1830 1842 1928
Size: 452 467 23 335 246 306 12 86
Cuts at: 1928 2980 1
Size: 1052 449
Fragments arranged by size:
1052 467 452 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 207 107
Size: 100 3328
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1378 1559 1722 1378
Size: 181 163 3084
Fragments arranged by size:
3084 181 163
BtsI GCAGTG_nn'
Cuts at: 671 671
Size: 3428
DraI TTT'AAA
Cuts at: 427 427
Size: 3428
DrdI GACnn_nn'nnGTC
Cuts at: 1134 2478 1134
Size: 1344 2084
EaeI y'GGCC_r
Cuts at: 1013 1187 1578 1605 2950 1013
Size: 174 391 27 1345 1491
Fragments arranged by size:
1491 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1013 1013
Size: 3428
EarI CTCTTCn'nnn_
Cuts at: 1451 1661 1451
Size: 210 3218
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 237 1636 2378 2524 3238 237
Size: 1399 742 146 714 427
Fragments arranged by size:
1399 742 714 427 146
EcoICRI GAG'CTC
Cuts at: 440 440
Size: 3428
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1253 1685 2038 2700 1253
Size: 432 353 662 1981
Fragments arranged by size:
1981 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 237 567 237
Size: 330 3098
EcoRI G'AATT_C
Cuts at: 432 432
Size: 3428
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1447 1447
Size: 3428
FspI TGC'GCA
Cuts at: 1209 1209
Size: 3428
HaeII r_GCGC'y
Cuts at: 862 1110 2340 3111 862
Size: 248 1230 771 1179
Fragments arranged by size:
1230 1179 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1270 1300 1302 1332 1270
Size: 30 2 30 3366
Fragments arranged by size:
3366 30 30 2
HincII GTy'rAC
Cuts at: 467 467
Size: 3428
KasI G'GCGC_C
Cuts at: 858 1106 858
Size: 248 3180
KpnI G_GTAC'C
Cuts at: 448 448
Size: 3428
MluI A'CGCG_T
Cuts at: 2861 2861
Size: 3428
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2187 2371 2187
Size: 184 3244
MscI TGG'CCA
Cuts at: 1189 1189
Size: 3428
MslI CAynn'nnrTG
Cuts at: 1417 1544 1417
Size: 127 3301
MspA1I CmG'CkG
Cuts at: 539 853 1213 1997 2242 539
Size: 314 360 784 245 1725
Fragments arranged by size:
1725 784 360 314 245
NaeI GCC'GGC
Cuts at: 1609 1609
Size: 3428
NarI GG'CG_CC
Cuts at: 859 1107 859
Size: 248 3180
NcoI C'CATG_G
Cuts at: 107 207 107
Size: 100 3328
NgoMIV G'CCGG_C
Cuts at: 1607 1607
Size: 3428
NspI r_CATG'y
Cuts at: 481 981 2584 481
Size: 500 1603 1325
Fragments arranged by size:
1603 1325 500
PciI A'CATG_T
Cuts at: 2580 2580
Size: 3428
PmeI GTTT'AAAC
Cuts at: 427 427
Size: 3428
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1874 1906 1874
Size: 32 3396
PstI C_TGCA'G
Cuts at: 475 1160 475
Size: 685 2743
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3428
PvuII CAG'CTG
Cuts at: 853 1213 853
Size: 360 3068
RsrII CG'GwC_CG
Cuts at: 1623 1623
Size: 3428
SacI G_AGCT'C
Cuts at: 442 442
Size: 3428
SalI G'TCGA_C
Cuts at: 465 465
Size: 3428
SapI GCTCTTCn'nnn_
Cuts at: 1451 1661 1451
Size: 210 3218
SbfI CC_TGCA'GG
Cuts at: 475 475
Size: 3428
ScaI AGT'ACT
Cuts at: 2945 2945
Size: 3428
SfcI C'TryA_G
Cuts at: 33 471 1156 2124 2315 33
Size: 438 685 968 191 1146
Fragments arranged by size:
1146 968 685 438 191
SfoI GGC'GCC
Cuts at: 860 1108 860
Size: 248 3180
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3428
SmaI CCC'GGG
Cuts at: 450 450
Size: 3428
SmlI C'TyrA_G
Cuts at: 583 1498 2212 2474 583
Size: 915 714 262 1537
Fragments arranged by size:
1537 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3428
SphI G_CATG'C
Cuts at: 481 981 481
Size: 500 2928
StyI C'CwwG_G
Cuts at: 107 207 562 916 107
Size: 100 355 354 2619
Fragments arranged by size:
2619 355 354 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 100 1011 1144 1603 1812 100
Size: 911 133 459 209 1716
Fragments arranged by size:
1716 911 459 209 133
TatI w'GTAC_w
Cuts at: 329 2943 329
Size: 2614 814
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2945 2977 2945
Size: 32 3396
Tth111I GACn'n_nGTC
Cuts at: 1225 1225
Size: 3428
XbaI T'CTAG_A
Cuts at: 459 459
Size: 3428
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 214 923 214
Size: 709 2719
XmaI C'CCGG_G
Cuts at: 448 448
Size: 3428
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI
BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI