Restriction map for
pF1KB3406
(Circular) (Six-base) MAPSORT of: pF1KB3406.seq Check: 2761 from: 1 to: 4214
pF1KB3406 4214 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1252 1252
Size: 4214
Acc65I G'GTAC_C
Cuts at: 1230 1230
Size: 4214
AclI AA'CG_TT
Cuts at: 4054 4054
Size: 4214
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2039 2471 2824 3486 2039
Size: 432 353 662 2767
Fragments arranged by size:
2767 662 432 353
AflII C'TTAA_G
Cuts at: 199 199
Size: 4214
AflIII A'CryG_T
Cuts at: 1566 3366 3647 1566
Size: 1800 281 2133
Fragments arranged by size:
2133 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 453 1519 2957 453
Size: 1066 1438 1710
Fragments arranged by size:
1710 1438 1066
ApaLI G'TGCA_C
Cuts at: 3052 3052
Size: 4214
ApoI r'AATT_y
Cuts at: 391 936 986 1102 1218 3567 3823 391
Size: 545 50 116 116 2349 256 782
Fragments arranged by size:
2349 782 545 256 116 116 50
AseI AT'TA_AT
Cuts at: 20 674 20
Size: 654 3560
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4214
AvaI C'yCGr_G
Cuts at: 1234 1234
Size: 4214
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 420 453 420
Size: 33 4181
BamHI G'GATC_C
Cuts at: 1239 1239
Size: 4214
BanI G'GyrC_C
Cuts at: 436 478 897 1230 1644 1892 1927 436
Size: 42 419 333 414 248 35 2723
Fragments arranged by size:
2723 419 414 333 248 42 35
BanII G_rGCy'C
Cuts at: 1228 2258 1228
Size: 1030 3184
BbeI G_GCGC'C
Cuts at: 1648 1896 1648
Size: 248 3966
BciVI GTATCCnnnnn_n'
Cuts at: 2112 3168 4214 2112
Size: 1056 1046 2112
Fragments arranged by size:
2112 1056 1046
BclI T'GATC_A
Cuts at: 1734 1734
Size: 4214
BglII A'GATC_T
Cuts at: 959 1729 3766 959
Size: 770 2037 1407
Fragments arranged by size:
2037 1407 770
BlpI GC'TnA_GC
Cuts at: 215 1326 215
Size: 1111 3103
Bme1580I G_kGCm'C
Cuts at: 1839 1932 3056 1839
Size: 93 1124 2997
Fragments arranged by size:
2997 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1024 1426 1507 1842 3986 1024
Size: 402 81 335 2144 1252
Fragments arranged by size:
2144 1252 402 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2581 2581
Size: 4214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1032 1032
Size: 4214
Bpu10I CC'TnA_GC
Cuts at: 120 120
Size: 4214
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1390 2269 2983 3281 1390
Size: 879 714 298 2323
Fragments arranged by size:
2323 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1132 3918 1132
Size: 2786 1428
BsaAI yAC'GTr
Cuts at: 772 1567 772
Size: 795 3419
BsaBI GATnn'nnATC
Cuts at: 6 1728 3765 6
Size: 1722 2037 455
Fragments arranged by size:
2037 1722 455
BsaHI Gr'CG_yC
Cuts at: 1645 1893 2595 4091 1645
Size: 248 702 1496 1768
Fragments arranged by size:
1768 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1403 1924 2602 3013 3160 3434 1403
Size: 521 678 411 147 274 2183
Fragments arranged by size:
2183 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 850 880 850
Size: 30 4184
BseYI C'CCAG_C
Cuts at: 1639 2397 3062 3470 1639
Size: 758 665 408 2383
Fragments arranged by size:
2383 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1756 1802 3032 104
Size: 1652 46 1230 1286
Fragments arranged by size:
1652 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1228 2006 2196 3056 1228
Size: 778 190 860 2386
Fragments arranged by size:
2386 860 778 190
BsmI GAATG_Cn'
Cuts at: 614 728 614
Size: 114 4100
Bsp1286I G_dGCh'C
Cuts at: 1228 1839 1932 2006 2196 2258 3056 1228
Size: 611 93 74 190 62 798 2386
Fragments arranged by size:
2386 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 2646 2646
Size: 4214
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1264 1478 1780 2161 1264
Size: 214 302 381 3317
Fragments arranged by size:
3317 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2506 2560 4044 4209 50
Size: 2456 54 1484 165 55
Fragments arranged by size:
2456 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 512 2126 512
Size: 1614 2600
BsrFI r'CCGG_y
Cuts at: 2212 2393 2212
Size: 181 4033
BssSI C'ACGA_G
Cuts at: 2485 3193 2485
Size: 708 3506
BstAPI GCAn_nnn'nTGC
Cuts at: 1591 1591
Size: 4214
BstBI TT'CG_AA
Cuts at: 2575 2575
Size: 4214
BstYI r'GATC_y
Cuts at: 1 443 456 959 1239 1706 1729 2064 2310
Size: 442 13 503 280 467 23 335 246
Cuts at: 2310 2616 2628 2714 3766 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 503 467 449 442 335 306 280
246 86 23 13 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2164 2345 2508 2164
Size: 181 163 3870
Fragments arranged by size:
3870 181 163
BtsI GCAGTG_nn'
Cuts at: 343 524 1457 343
Size: 181 933 3100
Fragments arranged by size:
3100 933 181
DraI TTT'AAA
Cuts at: 1107 1213 1107
Size: 106 4108
DrdI GACnn_nn'nnGTC
Cuts at: 1920 3264 1920
Size: 1344 2870
EaeI y'GGCC_r
Cuts at: 284 1799 1973 2364 2391 3736 284
Size: 1515 174 391 27 1345 762
Fragments arranged by size:
1515 1345 762 391 174 27
EagI C'GGCC_G
Cuts at: 1799 1799
Size: 4214
EarI CTCTTCn'nnn_
Cuts at: 718 2237 2447 718
Size: 1519 210 2485
Fragments arranged by size:
2485 1519 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2422 3164 3310 4024 2422
Size: 742 146 714 2612
Fragments arranged by size:
2612 742 714 146
EcoICRI GAG'CTC
Cuts at: 1226 1226
Size: 4214
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1032 2039 2471 2824 3486 1032
Size: 1007 432 353 662 1760
Fragments arranged by size:
1760 1007 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1353 1353
Size: 4214
EcoRI G'AATT_C
Cuts at: 391 1218 391
Size: 827 3387
EcoRV GAT'ATC
Cuts at: 885 885
Size: 4214
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2233 2233
Size: 4214
FspI TGC'GCA
Cuts at: 1995 1995
Size: 4214
HaeII r_GCGC'y
Cuts at: 1648 1896 3126 3897 1648
Size: 248 1230 771 1965
Fragments arranged by size:
1965 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 812 844 850 882 1009 1041 2056 2086 2088
Size: 32 6 32 127 32 1015 30 2
Cuts at: 2088 2118 812
Size: 30 2908
Fragments arranged by size:
2908 1015 127 32 32 32 30 30
6 2
HincII GTy'rAC
Cuts at: 341 1253 341
Size: 912 3302
HindIII A'AGCT_T
Cuts at: 217 217
Size: 4214
KasI G'GCGC_C
Cuts at: 1644 1892 1644
Size: 248 3966
KpnI G_GTAC'C
Cuts at: 1234 1234
Size: 4214
MluI A'CGCG_T
Cuts at: 3647 3647
Size: 4214
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1154 2973 3157 1154
Size: 1819 184 2211
Fragments arranged by size:
2211 1819 184
MscI TGG'CCA
Cuts at: 286 1975 286
Size: 1689 2525
MslI CAynn'nnrTG
Cuts at: 517 802 2203 2330 517
Size: 285 1401 127 2401
Fragments arranged by size:
2401 1401 285 127
MspA1I CmG'CkG
Cuts at: 787 932 1325 1639 1999 2783 3028 787
Size: 145 393 314 360 784 245 1973
Fragments arranged by size:
1973 784 393 360 314 245 145
NaeI GCC'GGC
Cuts at: 2395 2395
Size: 4214
NarI GG'CG_CC
Cuts at: 1645 1893 1645
Size: 248 3966
NgoMIV G'CCGG_C
Cuts at: 2393 2393
Size: 4214
NspI r_CATG'y
Cuts at: 1267 1767 3370 1267
Size: 500 1603 2111
Fragments arranged by size:
2111 1603 500
PciI A'CATG_T
Cuts at: 3366 3366
Size: 4214
PflMI CCAn_nnn'nTGG
Cuts at: 247 453 1173 247
Size: 206 720 3288
Fragments arranged by size:
3288 720 206
PfoI T'CCnGG_A
Cuts at: 264 452 1048 264
Size: 188 596 3430
Fragments arranged by size:
3430 596 188
PmeI GTTT'AAAC
Cuts at: 1213 1213
Size: 4214
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2660 2692 2660
Size: 32 4182
PstI C_TGCA'G
Cuts at: 1261 1946 1261
Size: 685 3529
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4214
PvuII CAG'CTG
Cuts at: 787 1639 1999 787
Size: 852 360 3002
Fragments arranged by size:
3002 852 360
RsrII CG'GwC_CG
Cuts at: 2409 2409
Size: 4214
SacI G_AGCT'C
Cuts at: 1228 1228
Size: 4214
SalI G'TCGA_C
Cuts at: 1251 1251
Size: 4214
SapI GCTCTTCn'nnn_
Cuts at: 718 2237 2447 718
Size: 1519 210 2485
Fragments arranged by size:
2485 1519 210
SbfI CC_TGCA'GG
Cuts at: 1261 1261
Size: 4214
ScaI AGT'ACT
Cuts at: 3731 3731
Size: 4214
SfcI C'TryA_G
Cuts at: 33 782 1257 1942 2910 3101 33
Size: 749 475 685 968 191 1146
Fragments arranged by size:
1146 968 749 685 475 191
SfoI GGC'GCC
Cuts at: 1646 1894 1646
Size: 248 3966
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4214
SmaI CCC'GGG
Cuts at: 1236 1236
Size: 4214
SmlI C'TyrA_G
Cuts at: 199 1369 2284 2998 3260 199
Size: 1170 915 714 262 1153
Fragments arranged by size:
1170 1153 915 714 262
SnaBI TAC'GTA
Cuts at: 772 772
Size: 4214
SpeI A'CTAG_T
Cuts at: 67 978 67
Size: 911 3303
SphI G_CATG'C
Cuts at: 1267 1767 1267
Size: 500 3714
StyI C'CwwG_G
Cuts at: 482 1348 1702 482
Size: 866 354 2994
Fragments arranged by size:
2994 866 354
SwaI ATTT'AAAT
Cuts at: 1107 1107
Size: 4214
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1797 1930 2389 2598 1797
Size: 133 459 209 3413
Fragments arranged by size:
3413 459 209 133
TatI w'GTAC_w
Cuts at: 3729 3729
Size: 4214
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3731 3763 3731
Size: 32 4182
Tth111I GACn'n_nGTC
Cuts at: 2011 2011
Size: 4214
XbaI T'CTAG_A
Cuts at: 1245 1245
Size: 4214
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 876 1056 1709 876
Size: 180 653 3381
Fragments arranged by size:
3381 653 180
XmaI C'CCGG_G
Cuts at: 1234 1234
Size: 4214
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BseRI BsgI BsiWI BsmBI BspEI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI FseI FspAI HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI SspI StuI XhoI XmnI ZraI