Restriction map for
pF1KB3410
(Circular) (Six-base) MAPSORT of: pF1KB3410.seq Check: 9094 from: 1 to: 5402
pF1KB3410 5402 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 153 720 153
Size: 567 4835
AccI GT'mk_AC
Cuts at: 2440 2440
Size: 5402
Acc65I G'GTAC_C
Cuts at: 2418 2418
Size: 5402
AclI AA'CG_TT
Cuts at: 5242 5242
Size: 5402
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 517 613 1153 1338 1390 3227 3659 4012
Size: 141 96 540 185 52 1837 432 353
Cuts at: 4012 4674 376
Size: 662 1104
Fragments arranged by size:
1837 1104 662 540 432 353 185 141
96 52
AflIII A'CryG_T
Cuts at: 1361 2222 2754 4554 4835 1361
Size: 861 532 1800 281 1928
Fragments arranged by size:
1928 1800 861 532 281
AhdI GACnn_n'nnGTC
Cuts at: 516 516
Size: 5402
AleI CACnn'nnGTG
Cuts at: 2068 2068
Size: 5402
AlwNI CAG_nnn'CTG
Cuts at: 135 168 288 309 462 771 825 888 1014
Size: 33 120 21 153 309 54 63 126
Cuts at: 1014 1131 1623 2152 2707 4145 135
Size: 117 492 529 555 1438 1392
Fragments arranged by size:
1438 1392 555 529 492 309 153 126
120 117 63 54 33 21
ApaI G_GGCC'C
Cuts at: 908 908
Size: 5402
ApaLI G'TGCA_C
Cuts at: 1192 4240 1192
Size: 3048 2354
ApoI r'AATT_y
Cuts at: 1555 2266 2406 4755 5011 1555
Size: 711 140 2349 256 1946
Fragments arranged by size:
2349 1946 711 256 140
AseI AT'TA_AT
Cuts at: 20 20
Size: 5402
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5402
AvaI C'yCGr_G
Cuts at: 910 2422 910
Size: 1512 3890
AvrII C'CTAG_G
Cuts at: 1797 1797
Size: 5402
BamHI G'GATC_C
Cuts at: 119 500 650 2427 119
Size: 381 150 1777 3094
Fragments arranged by size:
3094 1777 381 150
BanI G'GyrC_C
Cuts at: 1336 1494 2418 2832 3080 3115 1336
Size: 158 924 414 248 35 3623
Fragments arranged by size:
3623 924 414 248 158 35
BanII G_rGCy'C
Cuts at: 273 688 908 2416 3446 273
Size: 415 220 1508 1030 2229
Fragments arranged by size:
2229 1508 1030 415 220
BbeI G_GCGC'C
Cuts at: 2836 3084 2836
Size: 248 5154
BbsI GAAGACnn'nnnn_
Cuts at: 817 1141 2040 817
Size: 324 899 4179
Fragments arranged by size:
4179 899 324
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1881 1915 1881
Size: 34 5368
BciVI GTATCCnnnnn_n'
Cuts at: 3300 4356 5402 3300
Size: 1056 1046 3300
Fragments arranged by size:
3300 1056 1046
BclI T'GATC_A
Cuts at: 2922 2922
Size: 5402
BfrBI ATG'CAT
Cuts at: 2140 2140
Size: 5402
BglI GCCn_nnn'nGGC
Cuts at: 673 673
Size: 5402
BglII A'GATC_T
Cuts at: 2917 4954 2917
Size: 2037 3365
BlpI GC'TnA_GC
Cuts at: 682 995 2514 682
Size: 313 1519 3570
Fragments arranged by size:
3570 1519 313
Bme1580I G_kGCm'C
Cuts at: 374 908 1196 1339 1350 2206 3027 3120 4244
Size: 534 288 143 11 856 821 93 1124
Cuts at: 4244 374
Size: 1532
Fragments arranged by size:
1532 1124 856 821 534 288 143 93
11
BmgBI CAC'GTC
Cuts at: 927 927
Size: 5402
BmrI ACTGGGnnnn_n'
Cuts at: 207 1024 1677 2614 2695 3030 5174 207
Size: 817 653 937 81 335 2144 435
Fragments arranged by size:
2144 937 817 653 435 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2067 2407 3769 2067
Size: 340 1362 3700
Fragments arranged by size:
3700 1362 340
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 373 436 703 760 1669 310
Size: 63 63 267 57 909 4043
Fragments arranged by size:
4043 909 267 63 63 57
Bpu10I CC'TnA_GC
Cuts at: 2002 2002
Size: 5402
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2578 3457 4171 4469 2578
Size: 879 714 298 3511
Fragments arranged by size:
3511 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2275 5106 2275
Size: 2831 2571
BsaAI yAC'GTr
Cuts at: 2171 2304 2755 2171
Size: 133 451 4818
Fragments arranged by size:
4818 451 133
BsaBI GATnn'nnATC
Cuts at: 6 2916 4953 6
Size: 2910 2037 455
Fragments arranged by size:
2910 2037 455
BsaHI Gr'CG_yC
Cuts at: 512 2833 3081 3783 5279 512
Size: 2321 248 702 1496 635
Fragments arranged by size:
2321 1496 702 635 248
BsaWI w'CCGG_w
Cuts at: 2591 3112 3790 4201 4348 4622 2591
Size: 521 678 411 147 274 3371
Fragments arranged by size:
3371 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 733 763 733
Size: 30 5372
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 568 1051 1112 1432 568
Size: 483 61 320 4538
Fragments arranged by size:
4538 483 320 61
BseYI C'CCAG_C
Cuts at: 128 281 366 496 668 677 1992 2152 2193
Size: 153 85 130 172 9 1315 160 41
Cuts at: 2193 2827 3585 4250 4658 128
Size: 634 758 665 408 872
Fragments arranged by size:
1315 872 758 665 634 408 172 160
153 130 85 41 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 1302 1421 1591 322
Size: 980 119 170 4133
Fragments arranged by size:
4133 980 170 119
BsiEI CG_ry'CG
Cuts at: 104 2944 2990 4220 104
Size: 2840 46 1230 1286
Fragments arranged by size:
2840 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 329 1001 1196 2416 3194 3384 4244 329
Size: 672 195 1220 778 190 860 1487
Fragments arranged by size:
1487 1220 860 778 672 195 190
BsiWI C'GTAC_G
Cuts at: 1161 1161
Size: 5402
BsmI GAATG_Cn'
Cuts at: 2142 2142
Size: 5402
BsmBI CGTCTCn'nnnn_
Cuts at: 137 703 137
Size: 566 4836
Bsp1286I G_dGCh'C
Cuts at: 273 329 374 688 908 1001 1196 1339 1350
Size: 56 45 314 220 93 195 143 11
Cuts at: 1350 2206 2416 3027 3120 3194 3384 3446 4244
Size: 856 210 611 93 74 190 62 798
Cuts at: 4244 273
Size: 1431
Fragments arranged by size:
1431 856 798 611 314 220 210 195
190 143 93 93 74 62 56 45
11
BspHI T'CATG_A
Cuts at: 3834 3834
Size: 5402
BspMI ACCTGCnnnn'nnnn_
Cuts at: 153 720 1838 2452 2666 2968 3349 153
Size: 567 1118 614 214 302 381 2206
Fragments arranged by size:
2206 1118 614 567 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3694 3748 5232 5397 50
Size: 3644 54 1484 165 55
Fragments arranged by size:
3644 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1470 3314 1470
Size: 1844 3558
BsrFI r'CCGG_y
Cuts at: 974 1391 1988 2362 3400 3581 974
Size: 417 597 374 1038 181 2795
Fragments arranged by size:
2795 1038 597 417 374 181
BsrGI T'GTAC_A
Cuts at: 446 446
Size: 5402
BssSI C'ACGA_G
Cuts at: 1603 3673 4381 1603
Size: 2070 708 2624
Fragments arranged by size:
2624 2070 708
BstAPI GCAn_nnn'nTGC
Cuts at: 309 825 1280 2779 309
Size: 516 455 1499 2932
Fragments arranged by size:
2932 1499 516 455
BstBI TT'CG_AA
Cuts at: 3763 3763
Size: 5402
BstEII G'GTnAC_C
Cuts at: 1089 1089
Size: 5402
BstXI CCAn_nnnn'nTGG
Cuts at: 249 849 2175 2292 2304 249
Size: 600 1326 117 12 3347
Fragments arranged by size:
3347 1326 600 117 12
BstYI r'GATC_y
Cuts at: 1 119 500 650 983 1292 2427 2894 2917
Size: 118 381 150 333 309 1135 467 23
Cuts at: 2917 3252 3498 3804 3816 3902 4954 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1135 1052 467 449 381 335 333 309
306 246 150 118 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 646 1124 646
Size: 478 4924
BtgI C'CryG_G
Cuts at: 107 232 1331 2285 107
Size: 125 1099 954 3224
Fragments arranged by size:
3224 1099 954 125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1884 3352 3533 3696 1884
Size: 1468 181 163 3590
Fragments arranged by size:
3590 1468 181 163
BtsI GCAGTG_nn'
Cuts at: 769 1290 1735 2645 769
Size: 521 445 910 3526
Fragments arranged by size:
3526 910 521 445
DraI TTT'AAA
Cuts at: 2401 2401
Size: 5402
DraIII CAC_nnn'GTG
Cuts at: 333 333
Size: 5402
DrdI GACnn_nn'nnGTC
Cuts at: 3108 4452 3108
Size: 1344 4058
EaeI y'GGCC_r
Cuts at: 174 794 890 953 1046 1525 2294 2987 3161
Size: 620 96 63 93 479 769 693 174
Cuts at: 3161 3552 3579 4924 174
Size: 391 27 1345 652
Fragments arranged by size:
1345 769 693 652 620 479 391 174
96 93 63 27
EagI C'GGCC_G
Cuts at: 2987 2987
Size: 5402
EarI CTCTTCn'nnn_
Cuts at: 1366 3425 3635 1366
Size: 2059 210 3133
Fragments arranged by size:
3133 2059 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 337 3610 4352 4498 5212 337
Size: 3273 742 146 714 527
Fragments arranged by size:
3273 742 714 527 146
EcoICRI GAG'CTC
Cuts at: 2414 2414
Size: 5402
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 373 376 436 517 613 703 760 1153
Size: 63 3 60 81 96 90 57 393
Cuts at: 1153 1338 1390 1669 3227 3659 4012 4674 310
Size: 185 52 279 1558 432 353 662 1038
Fragments arranged by size:
1558 1038 662 432 393 353 279 185
96 90 81 63 60 57 52 3
EcoNI CCTnn'n_nnAGG
Cuts at: 301 1591 301
Size: 1290 4112
EcoO109I rG'GnC_Cy
Cuts at: 294 905 1128 1584 1853 1976 1998 2184 2541
Size: 611 223 456 269 123 22 186 357
Cuts at: 2541 294
Size: 3155
Fragments arranged by size:
3155 611 456 357 269 223 186 123
22
EcoRI G'AATT_C
Cuts at: 2266 2406 2266
Size: 140 5262
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3421 3421
Size: 5402
FspI TGC'GCA
Cuts at: 3183 3183
Size: 5402
HaeII r_GCGC'y
Cuts at: 1546 2168 2836 3084 4314 5085 1546
Size: 622 668 248 1230 771 1863
Fragments arranged by size:
1863 1230 771 668 622 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 502 534 642 674 1063 1095 3244 3274 3276
Size: 32 108 32 389 32 2149 30 2
Cuts at: 3276 3306 502
Size: 30 2598
Fragments arranged by size:
2598 2149 389 108 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 510 1056 2441 510
Size: 546 1385 3471
Fragments arranged by size:
3471 1385 546
KasI G'GCGC_C
Cuts at: 2832 3080 2832
Size: 248 5154
KpnI G_GTAC'C
Cuts at: 2422 2422
Size: 5402
MfeI C'AATT_G
Cuts at: 480 480
Size: 5402
MluI A'CGCG_T
Cuts at: 4835 4835
Size: 5402
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4161 4345 4161
Size: 184 5218
MscI TGG'CCA
Cuts at: 2296 3163 2296
Size: 867 4535
MslI CAynn'nnrTG
Cuts at: 1781 2017 2068 2173 2290 3391 3518 1781
Size: 236 51 105 117 1101 127 3665
Fragments arranged by size:
3665 1101 236 127 117 105 51
MspA1I CmG'CkG
Cuts at: 135 407 428 672 681 822 888 1014 1533
Size: 272 21 244 9 141 66 126 519
Cuts at: 1533 2197 2513 2827 3187 3971 4216 135
Size: 664 316 314 360 784 245 1321
Fragments arranged by size:
1321 784 664 519 360 316 314 272
245 244 141 126 66 21 9
NaeI GCC'GGC
Cuts at: 3583 3583
Size: 5402
NarI GG'CG_CC
Cuts at: 2833 3081 2833
Size: 248 5154
NcoI C'CATG_G
Cuts at: 107 232 2285 107
Size: 125 2053 3224
Fragments arranged by size:
3224 2053 125
NdeI CA'TA_TG
Cuts at: 399 399
Size: 5402
NgoMIV G'CCGG_C
Cuts at: 3581 3581
Size: 5402
NsiI A_TGCA'T
Cuts at: 2142 2142
Size: 5402
NspI r_CATG'y
Cuts at: 1365 2226 2455 2955 4558 1365
Size: 861 229 500 1603 2209
Fragments arranged by size:
2209 1603 861 500 229
PciI A'CATG_T
Cuts at: 1361 2222 4554 1361
Size: 861 2332 2209
Fragments arranged by size:
2332 2209 861
PflMI CCAn_nnn'nTGG
Cuts at: 288 1904 288
Size: 1616 3786
PfoI T'CCnGG_A
Cuts at: 287 848 287
Size: 561 4841
PmeI GTTT'AAAC
Cuts at: 2401 2401
Size: 5402
PmlI CAC'GTG
Cuts at: 2304 2304
Size: 5402
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1408 1432 1440 1459 1464 1491 3848 3880 1408
Size: 24 8 19 5 27 2357 32 2930
Fragments arranged by size:
2930 2357 32 27 24 19 8 5
PpuMI rG'GwC_Cy
Cuts at: 1128 1853 1976 1998 1128
Size: 725 123 22 4532
Fragments arranged by size:
4532 725 123 22
PspOMI G'GGCC_C
Cuts at: 904 904
Size: 5402
PstI C_TGCA'G
Cuts at: 140 314 605 776 821 2148 2449 3134 140
Size: 174 291 171 45 1327 301 685 2408
Fragments arranged by size:
2408 1327 685 301 291 174 171 45
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5402
PvuII CAG'CTG
Cuts at: 135 672 681 822 888 1014 1533 2197 2827
Size: 537 9 141 66 126 519 664 630
Cuts at: 2827 3187 135
Size: 360 2350
Fragments arranged by size:
2350 664 630 537 519 360 141 126
66 9
RsrII CG'GwC_CG
Cuts at: 3597 3597
Size: 5402
SacI G_AGCT'C
Cuts at: 2416 2416
Size: 5402
SalI G'TCGA_C
Cuts at: 2439 2439
Size: 5402
SapI GCTCTTCn'nnn_
Cuts at: 3425 3635 3425
Size: 210 5192
SbfI CC_TGCA'GG
Cuts at: 2449 2449
Size: 5402
ScaI AGT'ACT
Cuts at: 619 2059 4919 619
Size: 1440 2860 1102
Fragments arranged by size:
2860 1440 1102
SexAI A'CCwGG_T
Cuts at: 329 329
Size: 5402
SfcI C'TryA_G
Cuts at: 33 136 310 601 772 817 931 1656 2144
Size: 103 174 291 171 45 114 725 488
Cuts at: 2144 2445 3130 4098 4289 33
Size: 301 685 968 191 1146
Fragments arranged by size:
1146 968 725 685 488 301 291 191
174 171 114 103 45
SfoI GGC'GCC
Cuts at: 2834 3082 2834
Size: 248 5154
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5402
SmaI CCC'GGG
Cuts at: 2424 2424
Size: 5402
SmlI C'TyrA_G
Cuts at: 2557 3472 4186 4448 2557
Size: 915 714 262 3511
Fragments arranged by size:
3511 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5402
SphI G_CATG'C
Cuts at: 2455 2955 2455
Size: 500 4902
StuI AGG'CCT
Cuts at: 745 745
Size: 5402
StyI C'CwwG_G
Cuts at: 107 232 1592 1797 2036 2285 2536 2890 107
Size: 125 1360 205 239 249 251 354 2619
Fragments arranged by size:
2619 1360 354 251 249 239 205 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 295 2985 3118 3577 3786 295
Size: 2690 133 459 209 1911
Fragments arranged by size:
2690 1911 459 209 133
TatI w'GTAC_w
Cuts at: 446 617 2057 2247 4917 446
Size: 171 1440 190 2670 931
Fragments arranged by size:
2670 1440 931 190 171
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1368 1400 4919 4951 1368
Size: 32 3519 32 1819
Fragments arranged by size:
3519 1819 32 32
Tth111I GACn'n_nGTC
Cuts at: 3199 3199
Size: 5402
XbaI T'CTAG_A
Cuts at: 2433 2433
Size: 5402
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 285 1783 1977 2897 285
Size: 1498 194 920 2790
Fragments arranged by size:
2790 1498 920 194
XmaI C'CCGG_G
Cuts at: 2422 2422
Size: 5402
XmnI GAAnn'nnTTC
Cuts at: 1624 1948 1624
Size: 324 5078
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AlfI AloI AscI BaeI
BbvCI BmtI BspEI BssHII BstZ17I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI NheI NotI NruI PacI
PasI PshAI PsiI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI