Restriction map for
pF1KB3411
(Circular) (Six-base) MAPSORT of: pF1KB3411.seq Check: 6518 from: 1 to: 5810
pF1KB3411 5810 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1553 1691 1553
Size: 138 5672
AccI GT'mk_AC
Cuts at: 2519 2598 2848 2519
Size: 79 250 5481
Fragments arranged by size:
5481 250 79
Acc65I G'GTAC_C
Cuts at: 2144 2826 2144
Size: 682 5128
AclI AA'CG_TT
Cuts at: 5650 5650
Size: 5810
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 670 754 811 1837 2000 2191 2279 3635
Size: 117 84 57 1026 163 191 88 1356
Cuts at: 3635 4067 4420 5082 553
Size: 432 353 662 1281
Fragments arranged by size:
1356 1281 1026 662 432 353 191 163
117 88 84 57
AflIII A'CryG_T
Cuts at: 1055 2522 3162 4962 5243 1055
Size: 1467 640 1800 281 1622
Fragments arranged by size:
1800 1622 1467 640 281
AleI CACnn'nnGTG
Cuts at: 946 1175 2329 2686 946
Size: 229 1154 357 4070
Fragments arranged by size:
4070 1154 357 229
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2575 2575
Size: 5810
AlwNI CAG_nnn'CTG
Cuts at: 823 1822 2439 3115 4553 823
Size: 999 617 676 1438 2080
Fragments arranged by size:
2080 1438 999 676 617
ApaI G_GGCC'C
Cuts at: 485 1288 2749 485
Size: 803 1461 3546
Fragments arranged by size:
3546 1461 803
ApaLI G'TGCA_C
Cuts at: 1844 2800 4648 1844
Size: 956 1848 3006
Fragments arranged by size:
3006 1848 956
ApoI r'AATT_y
Cuts at: 2814 5163 5419 2814
Size: 2349 256 3205
Fragments arranged by size:
3205 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5810
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5810
AvaI C'yCGr_G
Cuts at: 1302 1436 1481 2663 2830 1302
Size: 134 45 1182 167 4282
Fragments arranged by size:
4282 1182 167 134 45
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2128 2161 2128
Size: 33 5777
BamHI G'GATC_C
Cuts at: 2835 2835
Size: 5810
BanI G'GyrC_C
Cuts at: 307 1338 2144 2783 2826 3240 3488 3523 307
Size: 1031 806 639 43 414 248 35 2594
Fragments arranged by size:
2594 1031 806 639 414 248 43 35
BanII G_rGCy'C
Cuts at: 485 1288 1437 1488 1866 1938 2210 2749 2774
Size: 803 149 51 378 72 272 539 25
Cuts at: 2774 2824 3854 485
Size: 50 1030 2441
Fragments arranged by size:
2441 1030 803 539 378 272 149 72
51 50 25
BbeI G_GCGC'C
Cuts at: 3244 3492 3244
Size: 248 5562
BbsI GAAGACnn'nnnn_
Cuts at: 1103 2341 2702 1103
Size: 1238 361 4211
Fragments arranged by size:
4211 1238 361
BbvCI CC'TCA_GC
Cuts at: 2647 2647
Size: 5810
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1417 1451 1417
Size: 34 5776
BciVI GTATCCnnnnn_n'
Cuts at: 1922 3708 4764 5810 1922
Size: 1786 1056 1046 1922
Fragments arranged by size:
1922 1786 1056 1046
BclI T'GATC_A
Cuts at: 1712 3330 1712
Size: 1618 4192
BfrBI ATG'CAT
Cuts at: 1800 1800
Size: 5810
BglI GCCn_nnn'nGGC
Cuts at: 1292 1460 1292
Size: 168 5642
BglII A'GATC_T
Cuts at: 350 3325 5362 350
Size: 2975 2037 798
Fragments arranged by size:
2975 2037 798
BlpI GC'TnA_GC
Cuts at: 2922 2922
Size: 5810
Bme1580I G_kGCm'C
Cuts at: 485 1288 1343 1848 1877 2218 2749 2786 2804
Size: 803 55 505 29 341 531 37 18
Cuts at: 2804 3435 3528 4652 485
Size: 631 93 1124 1643
Fragments arranged by size:
1643 1124 803 631 531 505 341 93
55 37 29 18
BmgBI CAC'GTC
Cuts at: 1056 1056
Size: 5810
BmrI ACTGGGnnnn_n'
Cuts at: 1392 2149 2540 3022 3103 3438 5582 1392
Size: 757 391 482 81 335 2144 1620
Fragments arranged by size:
2144 1620 757 482 391 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1507 1880 4177 1507
Size: 373 2297 3140
Fragments arranged by size:
3140 2297 373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 297 649 1238 1371 2461 2623 297
Size: 352 589 133 1090 162 3484
Fragments arranged by size:
3484 1090 589 352 162 133
Bpu10I CC'TnA_GC
Cuts at: 245 1832 2647 2756 245
Size: 1587 815 109 3299
Fragments arranged by size:
3299 1587 815 109
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2986 3865 4579 4877 2986
Size: 879 714 298 3919
Fragments arranged by size:
3919 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5514 5514
Size: 5810
BsaAI yAC'GTr
Cuts at: 2525 3163 2525
Size: 638 5172
BsaBI GATnn'nnATC
Cuts at: 6 3324 5361 6
Size: 3318 2037 455
Fragments arranged by size:
3318 2037 455
BsaHI Gr'CG_yC
Cuts at: 1502 1693 3241 3489 4191 5687 1502
Size: 191 1548 248 702 1496 1625
Fragments arranged by size:
1625 1548 1496 702 248 191
BsaWI w'CCGG_w
Cuts at: 974 2999 3520 4198 4609 4756 5030 974
Size: 2025 521 678 411 147 274 1754
Fragments arranged by size:
2025 1754 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 827 857 1040 1070 2035 2065 2197 2227 2596
Size: 30 183 30 965 30 132 30 369
Cuts at: 2596 2626 827
Size: 30 4011
Fragments arranged by size:
4011 965 369 183 132 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 162 1061 1226 1235 1239 2056 2218 2644 162
Size: 899 165 9 4 817 162 426 3328
Fragments arranged by size:
3328 899 817 426 165 162 9 4
BseYI C'CCAG_C
Cuts at: 456 749 1148 1342 1764 1877 2046 2389 2394
Size: 293 399 194 422 113 169 343 5
Cuts at: 2394 2477 2677 2742 2789 3235 3993 4658 5066
Size: 83 200 65 47 446 758 665 408
Cuts at: 5066 456
Size: 1200
Fragments arranged by size:
1200 758 665 446 422 408 399 343
293 200 194 169 113 83 65 47
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 886 1132 1214 1567 1669 1684 1778 2107 886
Size: 246 82 353 102 15 94 329 4589
Fragments arranged by size:
4589 353 329 246 102 94 82 15
BsiEI CG_ry'CG
Cuts at: 104 3352 3398 4628 104
Size: 3248 46 1230 1286
Fragments arranged by size:
3248 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 420 1437 1830 1848 1866 2804 2824 3602 3792
Size: 1017 393 18 18 938 20 778 190
Cuts at: 3792 4652 420
Size: 860 1578
Fragments arranged by size:
1578 1017 938 860 778 393 190 20
18 18
BsiWI C'GTAC_G
Cuts at: 2705 2705
Size: 5810
Bsp1286I G_dGCh'C
Cuts at: 420 485 1288 1343 1437 1488 1830 1848 1866
Size: 65 803 55 94 51 342 18 18
Cuts at: 1866 1877 1938 2210 2218 2749 2774 2786 2804
Size: 11 61 272 8 531 25 12 18
Cuts at: 2804 2824 3435 3528 3602 3792 3854 4652 420
Size: 20 611 93 74 190 62 798 1578
Fragments arranged by size:
1578 803 798 611 531 342 272 190
94 93 74 65 62 61 55 51
25 20 18 18 18 12 11 8
BspEI T'CCGG_A
Cuts at: 974 974
Size: 5810
BspHI T'CATG_A
Cuts at: 4242 4242
Size: 5810
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1553 1691 2584 2860 3074 3376 3757 1553
Size: 138 893 276 214 302 381 3606
Fragments arranged by size:
3606 893 381 302 276 214 138
BsrBI CCG'CTC
Cuts at: 50 587 1008 1998 4102 4156 5640 5805 50
Size: 537 421 990 2104 54 1484 165 55
Fragments arranged by size:
2104 1484 990 537 421 165 55 54
BsrDI GCAATG_nn'
Cuts at: 451 3722 451
Size: 3271 2539
BsrFI r'CCGG_y
Cuts at: 3808 3989 3808
Size: 181 5629
BssHII G'CGCG_C
Cuts at: 1450 1516 1518 1520 1450
Size: 66 2 2 5740
Fragments arranged by size:
5740 66 2 2
BssSI C'ACGA_G
Cuts at: 413 4081 4789 413
Size: 3668 708 1434
Fragments arranged by size:
3668 1434 708
BstAPI GCAn_nnn'nTGC
Cuts at: 446 3187 446
Size: 2741 3069
BstBI TT'CG_AA
Cuts at: 4171 4171
Size: 5810
BstEII G'GTnAC_C
Cuts at: 2055 2067 2055
Size: 12 5798
BstXI CCAn_nnnn'nTGG
Cuts at: 1177 1803 2037 1177
Size: 626 234 4950
Fragments arranged by size:
4950 626 234
BstYI r'GATC_y
Cuts at: 1 350 461 663 1956 2835 3302 3325 3660
Size: 349 111 202 1293 879 467 23 335
Cuts at: 3660 3906 4212 4224 4310 5362 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1293 1052 879 467 449 349 335 306
246 202 111 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2795 2795
Size: 5810
BtgI C'CryG_G
Cuts at: 107 291 1289 1334 1367 1508 1599 2366 2778
Size: 184 998 45 33 141 91 767 412
Cuts at: 2778 107
Size: 3139
Fragments arranged by size:
3139 998 767 412 184 141 91 45
33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3760 3941 4104 3760
Size: 181 163 5466
Fragments arranged by size:
5466 181 163
BtsI GCAGTG_nn'
Cuts at: 1579 1846 2218 3053 1579
Size: 267 372 835 4336
Fragments arranged by size:
4336 835 372 267
DraI TTT'AAA
Cuts at: 2809 2809
Size: 5810
DraIII CAC_nnn'GTG
Cuts at: 403 415 2419 2548 403
Size: 12 2004 129 3665
Fragments arranged by size:
3665 2004 129 12
DrdI GACnn_nn'nnGTC
Cuts at: 1501 3516 4860 1501
Size: 2015 1344 2451
Fragments arranged by size:
2451 2015 1344
EaeI y'GGCC_r
Cuts at: 1328 3395 3569 3960 3987 5332 1328
Size: 2067 174 391 27 1345 1806
Fragments arranged by size:
2067 1806 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3395 3395
Size: 5810
EarI CTCTTCn'nnn_
Cuts at: 1100 3833 4043 1100
Size: 2733 210 2867
Fragments arranged by size:
2867 2733 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4018 4760 4906 5620 4018
Size: 742 146 714 4208
Fragments arranged by size:
4208 742 714 146
EcoICRI GAG'CTC
Cuts at: 1435 1864 2822 1435
Size: 429 958 4423
Fragments arranged by size:
4423 958 429
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 297 553 649 670 754 811 1238 1371 1837
Size: 256 96 21 84 57 427 133 466
Cuts at: 1837 2000 2191 2279 2461 2623 3635 4067 4420
Size: 163 191 88 182 162 1012 432 353
Cuts at: 4420 5082 297
Size: 662 1025
Fragments arranged by size:
1025 1012 662 466 432 427 353 256
191 182 163 162 133 96 88 84
57 21
EcoNI CCTnn'n_nnAGG
Cuts at: 607 2170 2629 607
Size: 1563 459 3788
Fragments arranged by size:
3788 1563 459
EcoO109I rG'GnC_Cy
Cuts at: 283 481 593 600 1285 1391 1413 1653 2263
Size: 198 112 7 685 106 22 240 610
Cuts at: 2263 2586 2607 2949 283
Size: 323 21 342 3144
Fragments arranged by size:
3144 685 610 342 323 240 198 112
106 22 21 7
EcoRI G'AATT_C
Cuts at: 2814 2814
Size: 5810
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3829 3829
Size: 5810
FspI TGC'GCA
Cuts at: 2509 2563 3591 2509
Size: 54 1028 4728
Fragments arranged by size:
4728 1028 54
HaeII r_GCGC'y
Cuts at: 1382 1481 1542 1810 1823 3244 3492 4722 5493
Size: 99 61 268 13 1421 248 1230 771
Cuts at: 5493 1382
Size: 1699
Fragments arranged by size:
1699 1421 1230 771 268 248 99 61
13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 754 786 2233 2239 2265 2271 2596 2628 3652
Size: 32 1447 6 26 6 325 32 1024
Cuts at: 3652 3682 3684 3714 754
Size: 30 2 30 2850
Fragments arranged by size:
2850 1447 1024 325 32 32 30 30
26 6 6 2
HincII GTy'rAC
Cuts at: 1668 2849 1668
Size: 1181 4629
KasI G'GCGC_C
Cuts at: 3240 3488 3240
Size: 248 5562
KpnI G_GTAC'C
Cuts at: 2148 2830 2148
Size: 682 5128
MluI A'CGCG_T
Cuts at: 5243 5243
Size: 5810
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4569 4753 4569
Size: 184 5626
MscI TGG'CCA
Cuts at: 3571 3571
Size: 5810
MslI CAynn'nnrTG
Cuts at: 946 1175 2035 2329 2686 3799 3926 946
Size: 229 860 294 357 1113 127 2830
Fragments arranged by size:
2830 1113 860 357 294 229 127
MspA1I CmG'CkG
Cuts at: 211 579 621 1116 1369 1510 1881 2134 2448
Size: 368 42 495 253 141 371 253 314
Cuts at: 2448 2477 2921 3235 3595 4379 4624 211
Size: 29 444 314 360 784 245 1397
Fragments arranged by size:
1397 784 495 444 371 368 360 314
314 253 253 245 141 42 29
NaeI GCC'GGC
Cuts at: 3991 3991
Size: 5810
NarI GG'CG_CC
Cuts at: 3241 3489 3241
Size: 248 5562
NcoI C'CATG_G
Cuts at: 107 291 1289 2366 107
Size: 184 998 1077 3551
Fragments arranged by size:
3551 1077 998 184
NdeI CA'TA_TG
Cuts at: 1889 1889
Size: 5810
NgoMIV G'CCGG_C
Cuts at: 3989 3989
Size: 5810
NsiI A_TGCA'T
Cuts at: 1802 1802
Size: 5810
NspI r_CATG'y
Cuts at: 529 2863 3363 4966 529
Size: 2334 500 1603 1373
Fragments arranged by size:
2334 1603 1373 500
PasI CC'CwG_GG
Cuts at: 597 2469 597
Size: 1872 3938
PciI A'CATG_T
Cuts at: 4962 4962
Size: 5810
PflMI CCAn_nnn'nTGG
Cuts at: 432 2721 432
Size: 2289 3521
PfoI T'CCnGG_A
Cuts at: 1387 1387
Size: 5810
PmeI GTTT'AAAC
Cuts at: 2809 2809
Size: 5810
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 874 906 4256 4288 874
Size: 32 3350 32 2396
Fragments arranged by size:
3350 2396 32 32
PpuMI rG'GwC_Cy
Cuts at: 1391 1653 2586 1391
Size: 262 933 4615
Fragments arranged by size:
4615 933 262
PshAI GACnn'nnGTC
Cuts at: 1612 1612
Size: 5810
PspOMI G'GGCC_C
Cuts at: 481 1284 2745 481
Size: 803 1461 3546
Fragments arranged by size:
3546 1461 803
PstI C_TGCA'G
Cuts at: 1430 2435 2857 3542 1430
Size: 1005 422 685 3698
Fragments arranged by size:
3698 1005 685 422
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5810
PvuII CAG'CTG
Cuts at: 211 1881 2134 2448 2477 3235 3595 211
Size: 1670 253 314 29 758 360 2426
Fragments arranged by size:
2426 1670 758 360 314 253 29
RsrII CG'GwC_CG
Cuts at: 1929 4005 1929
Size: 2076 3734
SacI G_AGCT'C
Cuts at: 1437 1866 2824 1437
Size: 429 958 4423
Fragments arranged by size:
4423 958 429
SacII CC_GC'GG
Cuts at: 1370 1511 1370
Size: 141 5669
SalI G'TCGA_C
Cuts at: 2847 2847
Size: 5810
SapI GCTCTTCn'nnn_
Cuts at: 3833 4043 3833
Size: 210 5600
SbfI CC_TGCA'GG
Cuts at: 2857 2857
Size: 5810
ScaI AGT'ACT
Cuts at: 5327 5327
Size: 5810
SexAI A'CCwGG_T
Cuts at: 224 389 224
Size: 165 5645
SfcI C'TryA_G
Cuts at: 33 1260 1426 2431 2853 3538 4506 4697 33
Size: 1227 166 1005 422 685 968 191 1146
Fragments arranged by size:
1227 1146 1005 968 685 422 191 166
SfoI GGC'GCC
Cuts at: 3242 3490 3242
Size: 248 5562
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5810
SmaI CCC'GGG
Cuts at: 1304 2832 1304
Size: 1528 4282
SmlI C'TyrA_G
Cuts at: 1436 2965 3880 4594 4856 1436
Size: 1529 915 714 262 2390
Fragments arranged by size:
2390 1529 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5810
SphI G_CATG'C
Cuts at: 2863 3363 2863
Size: 500 5310
StuI AGG'CCT
Cuts at: 922 1132 922
Size: 210 5600
StyI C'CwwG_G
Cuts at: 107 188 291 485 1289 2327 2366 2944 3298
Size: 81 103 194 804 1038 39 578 354
Cuts at: 3298 107
Size: 2619
Fragments arranged by size:
2619 1038 804 578 354 194 103 81
39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 505 1165 2074 2559 3393 3526 3985 4194 505
Size: 660 909 485 834 133 459 209 2121
Fragments arranged by size:
2121 909 834 660 485 459 209 133
TatI w'GTAC_w
Cuts at: 1442 1917 5325 1442
Size: 475 3408 1927
Fragments arranged by size:
3408 1927 475
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1040 1072 2197 2229 5327 5359 1040
Size: 32 1125 32 3098 32 1491
Fragments arranged by size:
3098 1491 1125 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3607 3607
Size: 5810
XbaI T'CTAG_A
Cuts at: 2841 2841
Size: 5810
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 381 3305 381
Size: 2924 2886
XhoI C'TCGA_G
Cuts at: 1436 1436
Size: 5810
XmaI C'CCGG_G
Cuts at: 1302 2830 1302
Size: 1528 4282
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AloI AscI AvrII
BmtI BsmI BsmBI BsrGI BstZ17I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
PacI PmlI PsiI PspXI PsrI SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI XmnI ZraI