(Circular) (Six-base) MAPSORT of: pF1KB3419.seq Check: 9684 from: 1 to: 5513 pF1KB3419 5513 bp With 182 enzymes: SgfI * July 1, 2014 11:00 .. AccI GT'mk_AC Cuts at: 2551 2551 Size: 5513 Acc65I G'GTAC_C Cuts at: 2529 2529 Size: 5513 AclI AA'CG_TT Cuts at: 521 5353 521 Size: 4832 681 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 568 1163 1412 2093 3338 3770 4123 4785 568 Size: 595 249 681 1245 432 353 662 1296 Fragments arranged by size: 1296 1245 681 662 595 432 353 249 AflIII A'CryG_T Cuts at: 596 1689 2865 4665 4946 596 Size: 1093 1176 1800 281 1163 Fragments arranged by size: 1800 1176 1163 1093 281 AhdI GACnn_n'nnGTC Cuts at: 510 510 Size: 5513 AlwNI CAG_nnn'CTG Cuts at: 1456 2818 4256 1456 Size: 1362 1438 2713 Fragments arranged by size: 2713 1438 1362 ApaLI G'TGCA_C Cuts at: 1641 4351 1641 Size: 2710 2803 ApoI r'AATT_y Cuts at: 142 1344 2517 4866 5122 142 Size: 1202 1173 2349 256 533 Fragments arranged by size: 2349 1202 1173 533 256 AseI AT'TA_AT Cuts at: 20 20 Size: 5513 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5513 AvaI C'yCGr_G Cuts at: 2533 2533 Size: 5513 BamHI G'GATC_C Cuts at: 2538 2538 Size: 5513 BanI G'GyrC_C Cuts at: 121 565 1965 2529 2943 3191 3226 121 Size: 444 1400 564 414 248 35 2408 Fragments arranged by size: 2408 1400 564 444 414 248 35 BanII G_rGCy'C Cuts at: 2527 3557 2527 Size: 1030 4483 BbeI G_GCGC'C Cuts at: 2947 3195 2947 Size: 248 5265 BbsI GAAGACnn'nnnn_ Cuts at: 1136 1265 1136 Size: 129 5384 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1792 1826 1792 Size: 34 5479 BciVI GTATCCnnnnn_n' Cuts at: 3411 4467 5513 3411 Size: 1056 1046 3411 Fragments arranged by size: 3411 1056 1046 BclI T'GATC_A Cuts at: 3033 3033 Size: 5513 BfrBI ATG'CAT Cuts at: 462 462 Size: 5513 BglI GCCn_nnn'nGGC Cuts at: 2442 2442 Size: 5513 BglII A'GATC_T Cuts at: 551 3028 5065 551 Size: 2477 2037 999 Fragments arranged by size: 2477 2037 999 BlpI GC'TnA_GC Cuts at: 2625 2625 Size: 5513 Bme1580I G_kGCm'C Cuts at: 1645 1970 3138 3231 4355 1645 Size: 325 1168 93 1124 2803 Fragments arranged by size: 2803 1168 1124 325 93 BmgBI CAC'GTC Cuts at: 2155 2155 Size: 5513 BmrI ACTGGGnnnn_n' Cuts at: 2725 2806 3141 5285 2725 Size: 81 335 2144 2953 Fragments arranged by size: 2953 2144 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2382 3880 2382 Size: 1498 4015 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 614 907 1270 614 Size: 293 363 4857 Fragments arranged by size: 4857 363 293 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 493 1000 1791 2352 2689 3568 4282 4580 493 Size: 507 791 561 337 879 714 298 1426 Fragments arranged by size: 1426 879 791 714 561 507 337 298 BsaI GGTCTCn'nnnn_ Cuts at: 497 5217 497 Size: 4720 793 BsaAI yAC'GTr Cuts at: 2866 2866 Size: 5513 BsaBI GATnn'nnATC Cuts at: 6 419 3027 5064 6 Size: 413 2608 2037 455 Fragments arranged by size: 2608 2037 455 413 BsaHI Gr'CG_yC Cuts at: 2944 3192 3894 5390 2944 Size: 248 702 1496 3067 Fragments arranged by size: 3067 1496 702 248 BsaWI w'CCGG_w Cuts at: 2426 2702 3223 3901 4312 4459 4733 2426 Size: 276 521 678 411 147 274 3206 Fragments arranged by size: 3206 678 521 411 276 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 880 910 943 973 880 Size: 30 33 30 5420 Fragments arranged by size: 5420 33 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 238 421 238 Size: 183 5330 BseYI C'CCAG_C Cuts at: 2938 3696 4361 4769 2938 Size: 758 665 408 3682 Fragments arranged by size: 3682 758 665 408 BsiEI CG_ry'CG Cuts at: 104 3055 3101 4331 104 Size: 2951 46 1230 1286 Fragments arranged by size: 2951 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 1645 2527 3305 3495 4355 1645 Size: 882 778 190 860 2803 Fragments arranged by size: 2803 882 860 778 190 BsmI GAATG_Cn' Cuts at: 464 2211 464 Size: 1747 3766 BsmBI CGTCTCn'nnnn_ Cuts at: 119 1947 2021 119 Size: 1828 74 3611 Fragments arranged by size: 3611 1828 74 Bsp1286I G_dGCh'C Cuts at: 1645 1970 2527 3138 3231 3305 3495 3557 4355 Size: 325 557 611 93 74 190 62 798 Cuts at: 4355 1645 Size: 2803 Fragments arranged by size: 2803 798 611 557 325 190 93 74 62 BspHI T'CATG_A Cuts at: 3945 3945 Size: 5513 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1787 2563 2777 3079 3460 1787 Size: 776 214 302 381 3840 Fragments arranged by size: 3840 776 381 302 214 BsrBI CCG'CTC Cuts at: 50 3805 3859 5343 5508 50 Size: 3755 54 1484 165 55 Fragments arranged by size: 3755 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 3425 3425 Size: 5513 BsrFI r'CCGG_y Cuts at: 3511 3692 3511 Size: 181 5332 BssSI C'ACGA_G Cuts at: 906 3784 4492 906 Size: 2878 708 1927 Fragments arranged by size: 2878 1927 708 BstAPI GCAn_nnn'nTGC Cuts at: 2890 2890 Size: 5513 BstBI TT'CG_AA Cuts at: 3874 3874 Size: 5513 BstEII G'GTnAC_C Cuts at: 1901 1901 Size: 5513 BstXI CCAn_nnnn'nTGG Cuts at: 313 313 Size: 5513 BstYI r'GATC_y Cuts at: 1 551 2538 3005 3028 3363 3609 3915 3927 Size: 550 1987 467 23 335 246 306 12 Cuts at: 3927 4013 5065 1 Size: 86 1052 449 Fragments arranged by size: 1987 1052 550 467 449 335 306 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 1724 1724 Size: 5513 BtgI C'CryG_G Cuts at: 619 2490 619 Size: 1871 3642 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3463 3644 3807 3463 Size: 181 163 5169 Fragments arranged by size: 5169 181 163 BtsI GCAGTG_nn' Cuts at: 2756 2756 Size: 5513 DraI TTT'AAA Cuts at: 726 1052 1212 1571 2512 726 Size: 326 160 359 941 3727 Fragments arranged by size: 3727 941 359 326 160 DrdI GACnn_nn'nnGTC Cuts at: 1080 2392 3219 4563 1080 Size: 1312 827 1344 2030 Fragments arranged by size: 2030 1344 1312 827 EaeI y'GGCC_r Cuts at: 856 1926 3098 3272 3663 3690 5035 856 Size: 1070 1172 174 391 27 1345 1334 Fragments arranged by size: 1345 1334 1172 1070 391 174 27 EagI C'GGCC_G Cuts at: 3098 3098 Size: 5513 EarI CTCTTCn'nnn_ Cuts at: 1182 2055 2112 2375 3536 3746 1182 Size: 873 57 263 1161 210 2949 Fragments arranged by size: 2949 1161 873 263 210 57 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 964 3721 4463 4609 5323 964 Size: 2757 742 146 714 1154 Fragments arranged by size: 2757 1154 742 714 146 EcoICRI GAG'CTC Cuts at: 2525 2525 Size: 5513 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 568 614 907 1163 1270 1412 2093 3338 3770 Size: 46 293 256 107 142 681 1245 432 Cuts at: 3770 4123 4785 568 Size: 353 662 1296 Fragments arranged by size: 1296 1245 681 662 432 353 293 256 142 107 46 EcoNI CCTnn'n_nnAGG Cuts at: 2225 2225 Size: 5513 EcoO109I rG'GnC_Cy Cuts at: 717 2313 2652 717 Size: 1596 339 3578 Fragments arranged by size: 3578 1596 339 EcoRI G'AATT_C Cuts at: 2517 2517 Size: 5513 EcoRV GAT'ATC Cuts at: 215 215 Size: 5513 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3532 3532 Size: 5513 FspI TGC'GCA Cuts at: 3294 3294 Size: 5513 HaeII r_GCGC'y Cuts at: 2947 3195 4425 5196 2947 Size: 248 1230 771 3264 Fragments arranged by size: 3264 1230 771 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 183 215 496 528 880 912 3355 3385 3387 Size: 32 281 32 352 32 2443 30 2 Cuts at: 3387 3417 183 Size: 30 2279 Fragments arranged by size: 2443 2279 352 281 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 2552 2552 Size: 5513 HindIII A'AGCT_T Cuts at: 1203 1203 Size: 5513 KasI G'GCGC_C Cuts at: 2943 3191 2943 Size: 248 5265 KpnI G_GTAC'C Cuts at: 2533 2533 Size: 5513 MluI A'CGCG_T Cuts at: 4946 4946 Size: 5513 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 588 1109 1770 1826 1883 4272 4456 588 Size: 521 661 56 57 2389 184 1645 Fragments arranged by size: 2389 1645 661 521 184 57 56 MscI TGG'CCA Cuts at: 1928 3274 1928 Size: 1346 4167 MslI CAynn'nnrTG Cuts at: 1922 3502 3629 1922 Size: 1580 127 3806 Fragments arranged by size: 3806 1580 127 MspA1I CmG'CkG Cuts at: 1095 2624 2938 3298 4082 4327 1095 Size: 1529 314 360 784 245 2281 Fragments arranged by size: 2281 1529 784 360 314 245 NaeI GCC'GGC Cuts at: 3694 3694 Size: 5513 NarI GG'CG_CC Cuts at: 2944 3192 2944 Size: 248 5265 NcoI C'CATG_G Cuts at: 619 619 Size: 5513 NdeI CA'TA_TG Cuts at: 473 1653 473 Size: 1180 4333 NgoMIV G'CCGG_C Cuts at: 3692 3692 Size: 5513 NsiI A_TGCA'T Cuts at: 464 464 Size: 5513 NspI r_CATG'y Cuts at: 600 1693 2566 3066 4669 600 Size: 1093 873 500 1603 1444 Fragments arranged by size: 1603 1444 1093 873 500 PasI CC'CwG_GG Cuts at: 2309 2309 Size: 5513 PciI A'CATG_T Cuts at: 596 1689 4665 596 Size: 1093 2976 1444 Fragments arranged by size: 2976 1444 1093 PflMI CCAn_nnn'nTGG Cuts at: 1456 1456 Size: 5513 PfoI T'CCnGG_A Cuts at: 884 884 Size: 5513 PmeI GTTT'AAAC Cuts at: 2512 2512 Size: 5513 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1707 1739 3959 3991 1707 Size: 32 2220 32 3229 Fragments arranged by size: 3229 2220 32 32 PpuMI rG'GwC_Cy Cuts at: 717 2313 717 Size: 1596 3917 PstI C_TGCA'G Cuts at: 2560 3245 2560 Size: 685 4828 PvuI CG_AT'CG Cuts at: 104 104 Size: 5513 PvuII CAG'CTG Cuts at: 2938 3298 2938 Size: 360 5153 RsrII CG'GwC_CG Cuts at: 3708 3708 Size: 5513 SacI G_AGCT'C Cuts at: 2527 2527 Size: 5513 SalI G'TCGA_C Cuts at: 2550 2550 Size: 5513 SapI GCTCTTCn'nnn_ Cuts at: 3536 3746 3536 Size: 210 5303 SbfI CC_TGCA'GG Cuts at: 2560 2560 Size: 5513 ScaI AGT'ACT Cuts at: 545 5030 545 Size: 4485 1028 SfcI C'TryA_G Cuts at: 33 2250 2556 3241 4209 4400 33 Size: 2217 306 685 968 191 1146 Fragments arranged by size: 2217 1146 968 685 306 191 SfoI GGC'GCC Cuts at: 2945 3193 2945 Size: 248 5265 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5513 SmaI CCC'GGG Cuts at: 2535 2535 Size: 5513 SmlI C'TyrA_G Cuts at: 508 979 1806 2367 2668 3583 4297 4559 508 Size: 471 827 561 301 915 714 262 1462 Fragments arranged by size: 1462 915 827 714 561 471 301 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 5513 SphI G_CATG'C Cuts at: 2566 3066 2566 Size: 500 5013 StyI C'CwwG_G Cuts at: 602 619 1286 2647 3001 602 Size: 17 667 1361 354 3114 Fragments arranged by size: 3114 1361 667 354 17 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 3096 3229 3688 3897 3096 Size: 133 459 209 4712 Fragments arranged by size: 4712 459 209 133 TatI w'GTAC_w Cuts at: 543 5028 543 Size: 4485 1028 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 897 929 943 975 1459 1491 5030 5062 897 Size: 32 14 32 484 32 3539 32 1348 Fragments arranged by size: 3539 1348 484 32 32 32 32 14 Tth111I GACn'n_nGTC Cuts at: 3310 3310 Size: 5513 XbaI T'CTAG_A Cuts at: 2544 2544 Size: 5513 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3008 3008 Size: 5513 XmaI C'CCGG_G Cuts at: 2533 2533 Size: 5513 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AgeI AleI AlfI AloI ApaI AscI AvrII BaeI BbvCI BmtI Bpu10I BsgI BsiWI BspEI BsrGI BssHII BstZ17I ClaI CspCI DraIII FseI FspAI HpaI MfeI NheI NotI NruI PacI PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI