Restriction map for pF1KB3419
 (Circular) (Six-base) MAPSORT of: pF1KB3419.seq  Check: 9684  from: 1  to: 5513



pF1KB3419  5513 bp

 With 182 enzymes: SgfI * 

                        July 1, 2014 11:00  ..

AccI GT'mk_AC

Cuts at:   2551    2551 
   Size:       5513
 
Acc65I G'GTAC_C

Cuts at:   2529    2529 
   Size:       5513
 
AclI AA'CG_TT

Cuts at:    521    5353     521 
   Size:       4832     681
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    568    1163    1412    2093    3338    3770    4123    4785     568 
   Size:        595     249     681    1245     432     353     662    1296

  Fragments arranged by size: 

                1296    1245     681     662     595     432     353     249
            
 
AflIII A'CryG_T

Cuts at:    596    1689    2865    4665    4946     596 
   Size:       1093    1176    1800     281    1163

  Fragments arranged by size: 

                1800    1176    1163    1093     281
 
AhdI GACnn_n'nnGTC

Cuts at:    510     510 
   Size:       5513
 
AlwNI CAG_nnn'CTG

Cuts at:   1456    2818    4256    1456 
   Size:       1362    1438    2713

  Fragments arranged by size: 

                2713    1438    1362
 
ApaLI G'TGCA_C

Cuts at:   1641    4351    1641 
   Size:       2710    2803
 
ApoI r'AATT_y

Cuts at:    142    1344    2517    4866    5122     142 
   Size:       1202    1173    2349     256     533

  Fragments arranged by size: 

                2349    1202    1173     533     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5513
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5513
 
AvaI C'yCGr_G

Cuts at:   2533    2533 
   Size:       5513
 
BamHI G'GATC_C

Cuts at:   2538    2538 
   Size:       5513
 
BanI G'GyrC_C

Cuts at:    121     565    1965    2529    2943    3191    3226     121 
   Size:        444    1400     564     414     248      35    2408

  Fragments arranged by size: 

                2408    1400     564     444     414     248      35
 
BanII G_rGCy'C

Cuts at:   2527    3557    2527 
   Size:       1030    4483
 
BbeI G_GCGC'C

Cuts at:   2947    3195    2947 
   Size:        248    5265
 
BbsI GAAGACnn'nnnn_

Cuts at:   1136    1265    1136 
   Size:        129    5384
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1792    1826    1792 
   Size:         34    5479
 
BciVI GTATCCnnnnn_n'

Cuts at:   3411    4467    5513    3411 
   Size:       1056    1046    3411

  Fragments arranged by size: 

                3411    1056    1046
 
BclI T'GATC_A

Cuts at:   3033    3033 
   Size:       5513
 
BfrBI ATG'CAT

Cuts at:    462     462 
   Size:       5513
 
BglI GCCn_nnn'nGGC

Cuts at:   2442    2442 
   Size:       5513
 
BglII A'GATC_T

Cuts at:    551    3028    5065     551 
   Size:       2477    2037     999

  Fragments arranged by size: 

                2477    2037     999
 
BlpI GC'TnA_GC

Cuts at:   2625    2625 
   Size:       5513
 
Bme1580I G_kGCm'C

Cuts at:   1645    1970    3138    3231    4355    1645 
   Size:        325    1168      93    1124    2803

  Fragments arranged by size: 

                2803    1168    1124     325      93
 
BmgBI CAC'GTC

Cuts at:   2155    2155 
   Size:       5513
 
BmrI ACTGGGnnnn_n'

Cuts at:   2725    2806    3141    5285    2725 
   Size:         81     335    2144    2953

  Fragments arranged by size: 

                2953    2144     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2382    3880    2382 
   Size:       1498    4015
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    614     907    1270     614 
   Size:        293     363    4857

  Fragments arranged by size: 

                4857     363     293
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    493    1000    1791    2352    2689    3568    4282    4580     493 
   Size:        507     791     561     337     879     714     298    1426

  Fragments arranged by size: 

                1426     879     791     714     561     507     337     298
            
 
BsaI GGTCTCn'nnnn_

Cuts at:    497    5217     497 
   Size:       4720     793
 
BsaAI yAC'GTr

Cuts at:   2866    2866 
   Size:       5513
 
BsaBI GATnn'nnATC

Cuts at:      6     419    3027    5064       6 
   Size:        413    2608    2037     455

  Fragments arranged by size: 

                2608    2037     455     413
 
BsaHI Gr'CG_yC

Cuts at:   2944    3192    3894    5390    2944 
   Size:        248     702    1496    3067

  Fragments arranged by size: 

                3067    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   2426    2702    3223    3901    4312    4459    4733    2426 
   Size:        276     521     678     411     147     274    3206

  Fragments arranged by size: 

                3206     678     521     411     276     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    880     910     943     973     880 
   Size:         30      33      30    5420

  Fragments arranged by size: 

                5420      33      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    238     421     238 
   Size:        183    5330
 
BseYI C'CCAG_C

Cuts at:   2938    3696    4361    4769    2938 
   Size:        758     665     408    3682

  Fragments arranged by size: 

                3682     758     665     408
 
BsiEI CG_ry'CG

Cuts at:    104    3055    3101    4331     104 
   Size:       2951      46    1230    1286

  Fragments arranged by size: 

                2951    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:   1645    2527    3305    3495    4355    1645 
   Size:        882     778     190     860    2803

  Fragments arranged by size: 

                2803     882     860     778     190
 
BsmI GAATG_Cn'

Cuts at:    464    2211     464 
   Size:       1747    3766
 
BsmBI CGTCTCn'nnnn_

Cuts at:    119    1947    2021     119 
   Size:       1828      74    3611

  Fragments arranged by size: 

                3611    1828      74
 
Bsp1286I G_dGCh'C

Cuts at:   1645    1970    2527    3138    3231    3305    3495    3557    4355 
   Size:        325     557     611      93      74     190      62     798

Cuts at:   4355    1645 
   Size:       2803

  Fragments arranged by size: 

                2803     798     611     557     325     190      93      74
                  62
 
BspHI T'CATG_A

Cuts at:   3945    3945 
   Size:       5513
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1787    2563    2777    3079    3460    1787 
   Size:        776     214     302     381    3840

  Fragments arranged by size: 

                3840     776     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    3805    3859    5343    5508      50 
   Size:       3755      54    1484     165      55

  Fragments arranged by size: 

                3755    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   3425    3425 
   Size:       5513
 
BsrFI r'CCGG_y

Cuts at:   3511    3692    3511 
   Size:        181    5332
 
BssSI C'ACGA_G

Cuts at:    906    3784    4492     906 
   Size:       2878     708    1927

  Fragments arranged by size: 

                2878    1927     708
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2890    2890 
   Size:       5513
 
BstBI TT'CG_AA

Cuts at:   3874    3874 
   Size:       5513
 
BstEII G'GTnAC_C

Cuts at:   1901    1901 
   Size:       5513
 
BstXI CCAn_nnnn'nTGG

Cuts at:    313     313 
   Size:       5513
 
BstYI r'GATC_y

Cuts at:      1     551    2538    3005    3028    3363    3609    3915    3927 
   Size:        550    1987     467      23     335     246     306      12

Cuts at:   3927    4013    5065       1 
   Size:         86    1052     449

  Fragments arranged by size: 

                1987    1052     550     467     449     335     306     246
                  86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   1724    1724 
   Size:       5513
 
BtgI C'CryG_G

Cuts at:    619    2490     619 
   Size:       1871    3642
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3463    3644    3807    3463 
   Size:        181     163    5169

  Fragments arranged by size: 

                5169     181     163
 
BtsI GCAGTG_nn'

Cuts at:   2756    2756 
   Size:       5513
 
DraI TTT'AAA

Cuts at:    726    1052    1212    1571    2512     726 
   Size:        326     160     359     941    3727

  Fragments arranged by size: 

                3727     941     359     326     160
 
DrdI GACnn_nn'nnGTC

Cuts at:   1080    2392    3219    4563    1080 
   Size:       1312     827    1344    2030

  Fragments arranged by size: 

                2030    1344    1312     827
 
EaeI y'GGCC_r

Cuts at:    856    1926    3098    3272    3663    3690    5035     856 
   Size:       1070    1172     174     391      27    1345    1334

  Fragments arranged by size: 

                1345    1334    1172    1070     391     174      27
 
EagI C'GGCC_G

Cuts at:   3098    3098 
   Size:       5513
 
EarI CTCTTCn'nnn_

Cuts at:   1182    2055    2112    2375    3536    3746    1182 
   Size:        873      57     263    1161     210    2949

  Fragments arranged by size: 

                2949    1161     873     263     210      57
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    964    3721    4463    4609    5323     964 
   Size:       2757     742     146     714    1154

  Fragments arranged by size: 

                2757    1154     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   2525    2525 
   Size:       5513
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    568     614     907    1163    1270    1412    2093    3338    3770 
   Size:         46     293     256     107     142     681    1245     432

Cuts at:   3770    4123    4785     568 
   Size:        353     662    1296

  Fragments arranged by size: 

                1296    1245     681     662     432     353     293     256
                 142     107      46
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2225    2225 
   Size:       5513
 
EcoO109I rG'GnC_Cy

Cuts at:    717    2313    2652     717 
   Size:       1596     339    3578

  Fragments arranged by size: 

                3578    1596     339
 
EcoRI G'AATT_C

Cuts at:   2517    2517 
   Size:       5513
 
EcoRV GAT'ATC

Cuts at:    215     215 
   Size:       5513
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3532    3532 
   Size:       5513
 
FspI TGC'GCA

Cuts at:   3294    3294 
   Size:       5513
 
HaeII r_GCGC'y

Cuts at:   2947    3195    4425    5196    2947 
   Size:        248    1230     771    3264

  Fragments arranged by size: 

                3264    1230     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    183     215     496     528     880     912    3355    3385    3387 
   Size:         32     281      32     352      32    2443      30       2

Cuts at:   3387    3417     183 
   Size:         30    2279

  Fragments arranged by size: 

                2443    2279     352     281      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:   2552    2552 
   Size:       5513
 
HindIII A'AGCT_T

Cuts at:   1203    1203 
   Size:       5513
 
KasI G'GCGC_C

Cuts at:   2943    3191    2943 
   Size:        248    5265
 
KpnI G_GTAC'C

Cuts at:   2533    2533 
   Size:       5513
 
MluI A'CGCG_T

Cuts at:   4946    4946 
   Size:       5513
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    588    1109    1770    1826    1883    4272    4456     588 
   Size:        521     661      56      57    2389     184    1645

  Fragments arranged by size: 

                2389    1645     661     521     184      57      56
 
MscI TGG'CCA

Cuts at:   1928    3274    1928 
   Size:       1346    4167
 
MslI CAynn'nnrTG

Cuts at:   1922    3502    3629    1922 
   Size:       1580     127    3806

  Fragments arranged by size: 

                3806    1580     127
 
MspA1I CmG'CkG

Cuts at:   1095    2624    2938    3298    4082    4327    1095 
   Size:       1529     314     360     784     245    2281

  Fragments arranged by size: 

                2281    1529     784     360     314     245
 
NaeI GCC'GGC

Cuts at:   3694    3694 
   Size:       5513
 
NarI GG'CG_CC

Cuts at:   2944    3192    2944 
   Size:        248    5265
 
NcoI C'CATG_G

Cuts at:    619     619 
   Size:       5513
 
NdeI CA'TA_TG

Cuts at:    473    1653     473 
   Size:       1180    4333
 
NgoMIV G'CCGG_C

Cuts at:   3692    3692 
   Size:       5513
 
NsiI A_TGCA'T

Cuts at:    464     464 
   Size:       5513
 
NspI r_CATG'y

Cuts at:    600    1693    2566    3066    4669     600 
   Size:       1093     873     500    1603    1444

  Fragments arranged by size: 

                1603    1444    1093     873     500
 
PasI CC'CwG_GG

Cuts at:   2309    2309 
   Size:       5513
 
PciI A'CATG_T

Cuts at:    596    1689    4665     596 
   Size:       1093    2976    1444

  Fragments arranged by size: 

                2976    1444    1093
 
PflMI CCAn_nnn'nTGG

Cuts at:   1456    1456 
   Size:       5513
 
PfoI T'CCnGG_A

Cuts at:    884     884 
   Size:       5513
 
PmeI GTTT'AAAC

Cuts at:   2512    2512 
   Size:       5513
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1707    1739    3959    3991    1707 
   Size:         32    2220      32    3229

  Fragments arranged by size: 

                3229    2220      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    717    2313     717 
   Size:       1596    3917
 
PstI C_TGCA'G

Cuts at:   2560    3245    2560 
   Size:        685    4828
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5513
 
PvuII CAG'CTG

Cuts at:   2938    3298    2938 
   Size:        360    5153
 
RsrII CG'GwC_CG

Cuts at:   3708    3708 
   Size:       5513
 
SacI G_AGCT'C

Cuts at:   2527    2527 
   Size:       5513
 
SalI G'TCGA_C

Cuts at:   2550    2550 
   Size:       5513
 
SapI GCTCTTCn'nnn_

Cuts at:   3536    3746    3536 
   Size:        210    5303
 
SbfI CC_TGCA'GG

Cuts at:   2560    2560 
   Size:       5513
 
ScaI AGT'ACT

Cuts at:    545    5030     545 
   Size:       4485    1028
 
SfcI C'TryA_G

Cuts at:     33    2250    2556    3241    4209    4400      33 
   Size:       2217     306     685     968     191    1146

  Fragments arranged by size: 

                2217    1146     968     685     306     191
 
SfoI GGC'GCC

Cuts at:   2945    3193    2945 
   Size:        248    5265
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5513
 
SmaI CCC'GGG

Cuts at:   2535    2535 
   Size:       5513
 
SmlI C'TyrA_G

Cuts at:    508     979    1806    2367    2668    3583    4297    4559     508 
   Size:        471     827     561     301     915     714     262    1462

  Fragments arranged by size: 

                1462     915     827     714     561     471     301     262
            
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5513
 
SphI G_CATG'C

Cuts at:   2566    3066    2566 
   Size:        500    5013
 
StyI C'CwwG_G

Cuts at:    602     619    1286    2647    3001     602 
   Size:         17     667    1361     354    3114

  Fragments arranged by size: 

                3114    1361     667     354      17
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   3096    3229    3688    3897    3096 
   Size:        133     459     209    4712

  Fragments arranged by size: 

                4712     459     209     133
 
TatI w'GTAC_w

Cuts at:    543    5028     543 
   Size:       4485    1028
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    897     929     943     975    1459    1491    5030    5062     897 
   Size:         32      14      32     484      32    3539      32    1348

  Fragments arranged by size: 

                3539    1348     484      32      32      32      32      14
            
 
Tth111I GACn'n_nGTC

Cuts at:   3310    3310 
   Size:       5513
 
XbaI T'CTAG_A

Cuts at:   2544    2544 
   Size:       5513
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3008    3008 
   Size:       5513
 
XmaI C'CCGG_G

Cuts at:   2533    2533 
   Size:       5513
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AhdI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NsiI     NspI     PasI     PciI    PflMI     PfoI     PmeI     PpiI
    PpuMI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI    BbvCI     BmtI   Bpu10I     BsgI
    BsiWI    BspEI    BsrGI   BssHII  BstZ17I     ClaI    CspCI   DraIII
     FseI    FspAI     HpaI     MfeI     NheI     NotI     NruI     PacI
     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI
    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI
     XhoI     XmnI     ZraI
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