Restriction map for
pF1KB3422
(Circular) (Six-base) MAPSORT of: pF1KB3422.seq Check: 4274 from: 1 to: 3506
pF1KB3422 3506 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 544 544
Size: 3506
Acc65I G'GTAC_C
Cuts at: 522 522
Size: 3506
AclI AA'CG_TT
Cuts at: 3346 3346
Size: 3506
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1331 1763 2510 2778 1331
Size: 432 747 268 2059
Fragments arranged by size:
2059 747 432 268
AflIII A'CryG_T
Cuts at: 858 1962 2939 858
Size: 1104 977 1425
Fragments arranged by size:
1425 1104 977
AgeI A'CCGG_T
Cuts at: 1894 1894
Size: 3506
AleI CACnn'nnGTG
Cuts at: 299 299
Size: 3506
AlwNI CAG_nnn'CTG
Cuts at: 811 2378 811
Size: 1567 1939
ApaLI G'TGCA_C
Cuts at: 2276 2276
Size: 3506
ApoI r'AATT_y
Cuts at: 510 2859 3115 510
Size: 2349 256 901
Fragments arranged by size:
2349 901 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3506
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3506
AvaI C'yCGr_G
Cuts at: 202 526 202
Size: 324 3182
BamHI G'GATC_C
Cuts at: 531 531
Size: 3506
BanI G'GyrC_C
Cuts at: 522 936 1184 1219 522
Size: 414 248 35 2809
Fragments arranged by size:
2809 414 248 35
BanII G_rGCy'C
Cuts at: 199 260 520 1550 199
Size: 61 260 1030 2155
Fragments arranged by size:
2155 1030 260 61
BbeI G_GCGC'C
Cuts at: 940 1188 940
Size: 248 3258
BciVI GTATCCnnnnn_n'
Cuts at: 1404 2165 3506 1404
Size: 761 1341 1404
Fragments arranged by size:
1404 1341 761
BclI T'GATC_A
Cuts at: 1026 1026
Size: 3506
BglII A'GATC_T
Cuts at: 1021 3058 1021
Size: 2037 1469
BlpI GC'TnA_GC
Cuts at: 618 618
Size: 3506
Bme1580I G_kGCm'C
Cuts at: 1131 1224 2280 1131
Size: 93 1056 2357
Fragments arranged by size:
2357 1056 93
BmgBI CAC'GTC
Cuts at: 117 117
Size: 3506
BmrI ACTGGGnnnn_n'
Cuts at: 718 799 1134 3278 718
Size: 81 335 2144 946
Fragments arranged by size:
2144 946 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1873 1873
Size: 3506
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 1561 2053 2351 682
Size: 879 492 298 1837
Fragments arranged by size:
1837 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3210 3210
Size: 3506
BsaAI yAC'GTr
Cuts at: 859 859
Size: 3506
BsaBI GATnn'nnATC
Cuts at: 6 1020 3057 6
Size: 1014 2037 455
Fragments arranged by size:
2037 1014 455
BsaHI Gr'CG_yC
Cuts at: 937 1185 1887 3383 937
Size: 248 702 1496 1060
Fragments arranged by size:
1496 1060 702 248
BsaWI w'CCGG_w
Cuts at: 695 1216 1894 2168 2315 2726 695
Size: 521 678 274 147 411 1475
Fragments arranged by size:
1475 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 469 469
Size: 3506
BseYI C'CCAG_C
Cuts at: 931 1689 2266 2762 931
Size: 758 577 496 1675
Fragments arranged by size:
1675 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 332
Size: 3506
BsiEI CG_ry'CG
Cuts at: 104 1048 1094 2302 104
Size: 944 46 1208 1308
Fragments arranged by size:
1308 1208 944 46
BsiHKAI G_wGCw'C
Cuts at: 199 239 260 520 1298 1488 2280 199
Size: 40 21 260 778 190 792 1425
Fragments arranged by size:
1425 792 778 260 190 40 21
Bsp1286I G_dGCh'C
Cuts at: 199 239 260 520 1131 1224 1298 1488 1550
Size: 40 21 260 611 93 74 190 62
Cuts at: 1550 2280 199
Size: 730 1425
Fragments arranged by size:
1425 730 611 260 190 93 74 62
40 21
BspEI T'CCGG_A
Cuts at: 2726 2726
Size: 3506
BspHI T'CATG_A
Cuts at: 188 2682 188
Size: 2494 1012
BspMI ACCTGCnnnn'nnnn_
Cuts at: 556 770 1072 1453 556
Size: 214 302 381 2609
Fragments arranged by size:
2609 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1798 1852 3336 3501 50
Size: 1748 54 1484 165 55
Fragments arranged by size:
1748 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1418 1418
Size: 3506
BsrFI r'CCGG_y
Cuts at: 1504 1685 1894 1504
Size: 181 209 3116
Fragments arranged by size:
3116 209 181
BssSI C'ACGA_G
Cuts at: 1777 2135 1777
Size: 358 3148
BstAPI GCAn_nnn'nTGC
Cuts at: 346 883 346
Size: 537 2969
BstBI TT'CG_AA
Cuts at: 1867 1867
Size: 3506
BstYI r'GATC_y
Cuts at: 1 531 998 1021 1356 1602 2614 2700 2712
Size: 530 467 23 335 246 1012 86 12
Cuts at: 2712 3058 1
Size: 346 449
Fragments arranged by size:
1012 530 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 251 107
Size: 144 3362
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1456 1637 1800 1456
Size: 181 163 3162
Fragments arranged by size:
3162 181 163
BtsI GCAGTG_nn'
Cuts at: 306 344 749 306
Size: 38 405 3063
Fragments arranged by size:
3063 405 38
DraI TTT'AAA
Cuts at: 505 505
Size: 3506
DrdI GACnn_nn'nnGTC
Cuts at: 1212 2070 1212
Size: 858 2648
EaeI y'GGCC_r
Cuts at: 242 1091 1265 1656 1683 3028 242
Size: 849 174 391 27 1345 720
Fragments arranged by size:
1345 849 720 391 174 27
EagI C'GGCC_G
Cuts at: 1091 1091
Size: 3506
EarI CTCTTCn'nnn_
Cuts at: 187 1529 1739 187
Size: 1342 210 1954
Fragments arranged by size:
1954 1342 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 1714 2024 2170 3316 127
Size: 1587 310 146 1146 317
Fragments arranged by size:
1587 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 197 258 518 197
Size: 61 260 3185
Fragments arranged by size:
3185 260 61
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1331 1763 2510 2778 1331
Size: 432 747 268 2059
Fragments arranged by size:
2059 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 645 645
Size: 3506
EcoRI G'AATT_C
Cuts at: 510 510
Size: 3506
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1525 1525
Size: 3506
FspI TGC'GCA
Cuts at: 1287 1287
Size: 3506
HaeII r_GCGC'y
Cuts at: 940 1188 2210 3189 940
Size: 248 1022 979 1257
Fragments arranged by size:
1257 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1348 1378 1380 1410 1348
Size: 30 2 30 3444
Fragments arranged by size:
3444 30 30 2
HincII GTy'rAC
Cuts at: 545 545
Size: 3506
KasI G'GCGC_C
Cuts at: 936 1184 936
Size: 248 3258
KpnI G_GTAC'C
Cuts at: 526 526
Size: 3506
MluI A'CGCG_T
Cuts at: 2939 2939
Size: 3506
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2177 2361 2177
Size: 184 3322
MscI TGG'CCA
Cuts at: 1267 1267
Size: 3506
MslI CAynn'nnrTG
Cuts at: 299 1495 1622 299
Size: 1196 127 2183
Fragments arranged by size:
2183 1196 127
MspA1I CmG'CkG
Cuts at: 150 617 931 1291 2304 2549 150
Size: 467 314 360 1013 245 1107
Fragments arranged by size:
1107 1013 467 360 314 245
NaeI GCC'GGC
Cuts at: 1687 1687
Size: 3506
NarI GG'CG_CC
Cuts at: 937 1185 937
Size: 248 3258
NcoI C'CATG_G
Cuts at: 107 107
Size: 3506
NgoMIV G'CCGG_C
Cuts at: 1685 1685
Size: 3506
NspI r_CATG'y
Cuts at: 559 1059 1966 559
Size: 500 907 2099
Fragments arranged by size:
2099 907 500
PciI A'CATG_T
Cuts at: 1962 1962
Size: 3506
PmeI GTTT'AAAC
Cuts at: 505 505
Size: 3506
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2645 2677 2645
Size: 32 3474
PstI C_TGCA'G
Cuts at: 553 1238 553
Size: 685 2821
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3506
PvuII CAG'CTG
Cuts at: 931 1291 931
Size: 360 3146
RsrII CG'GwC_CG
Cuts at: 1701 1701
Size: 3506
SacI G_AGCT'C
Cuts at: 199 260 520 199
Size: 61 260 3185
Fragments arranged by size:
3185 260 61
SalI G'TCGA_C
Cuts at: 543 543
Size: 3506
SapI GCTCTTCn'nnn_
Cuts at: 187 1529 1739 187
Size: 1342 210 1954
Fragments arranged by size:
1954 1342 210
SbfI CC_TGCA'GG
Cuts at: 553 553
Size: 3506
ScaI AGT'ACT
Cuts at: 320 3023 320
Size: 2703 803
SfcI C'TryA_G
Cuts at: 33 288 549 1234 2227 2418 33
Size: 255 261 685 993 191 1121
Fragments arranged by size:
1121 993 685 261 255 191
SfoI GGC'GCC
Cuts at: 938 1186 938
Size: 248 3258
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3506
SmaI CCC'GGG
Cuts at: 528 528
Size: 3506
SmlI C'TyrA_G
Cuts at: 661 1576 2068 2330 661
Size: 915 492 262 1837
Fragments arranged by size:
1837 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3506
SphI G_CATG'C
Cuts at: 559 1059 559
Size: 500 3006
StyI C'CwwG_G
Cuts at: 107 245 640 994 107
Size: 138 395 354 2619
Fragments arranged by size:
2619 395 354 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1089 1222 1681 1890 1089
Size: 133 459 209 2705
Fragments arranged by size:
2705 459 209 133
TatI w'GTAC_w
Cuts at: 318 3021 318
Size: 2703 803
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3023 3055 3023
Size: 32 3474
Tth111I GACn'n_nGTC
Cuts at: 118 1303 118
Size: 1185 2321
XbaI T'CTAG_A
Cuts at: 537 537
Size: 3506
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1001 114
Size: 887 2619
XmaI C'CCGG_G
Cuts at: 526 526
Size: 3506
XmnI GAAnn'nnTTC
Cuts at: 365 365
Size: 3506
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmtI BpmI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI