Restriction map for
pF1KB3424
(Circular) (Six-base) MAPSORT of: pF1KB3424.seq Check: 4391 from: 1 to: 4433
pF1KB3424 4433 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 478 524 1471 478
Size: 46 947 3440
Fragments arranged by size:
3440 947 46
Acc65I G'GTAC_C
Cuts at: 1449 1449
Size: 4433
AclI AA'CG_TT
Cuts at: 4273 4273
Size: 4433
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 950 2258 2690 3043 3705 316
Size: 634 1308 432 353 662 1044
Fragments arranged by size:
1308 1044 662 634 432 353
AflII C'TTAA_G
Cuts at: 1326 1326
Size: 4433
AflIII A'CryG_T
Cuts at: 1785 3585 3866 1785
Size: 1800 281 2352
Fragments arranged by size:
2352 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 241 1312 1738 3176 241
Size: 1071 426 1438 1498
Fragments arranged by size:
1498 1438 1071 426
ApaI G_GGCC'C
Cuts at: 899 899
Size: 4433
ApaLI G'TGCA_C
Cuts at: 3271 3271
Size: 4433
ApoI r'AATT_y
Cuts at: 337 686 1019 1437 3786 4042 337
Size: 349 333 418 2349 256 728
Fragments arranged by size:
2349 728 418 349 333 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4433
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4433
AvaI C'yCGr_G
Cuts at: 1453 1453
Size: 4433
AvrII C'CTAG_G
Cuts at: 885 885
Size: 4433
BamHI G'GATC_C
Cuts at: 609 1119 1458 609
Size: 510 339 3584
Fragments arranged by size:
3584 510 339
BanI G'GyrC_C
Cuts at: 1405 1449 1863 2111 2146 1405
Size: 44 414 248 35 3692
Fragments arranged by size:
3692 414 248 44 35
BanII G_rGCy'C
Cuts at: 493 899 1447 2477 493
Size: 406 548 1030 2449
Fragments arranged by size:
2449 1030 548 406
BbeI G_GCGC'C
Cuts at: 1867 2115 1867
Size: 248 4185
BbsI GAAGACnn'nnnn_
Cuts at: 1364 1364
Size: 4433
BbvCI CC'TCA_GC
Cuts at: 837 837
Size: 4433
BciVI GTATCCnnnnn_n'
Cuts at: 2331 3387 4433 2331
Size: 1056 1046 2331
Fragments arranged by size:
2331 1056 1046
BclI T'GATC_A
Cuts at: 1256 1953 1256
Size: 697 3736
BglII A'GATC_T
Cuts at: 1948 3985 1948
Size: 2037 2396
BlpI GC'TnA_GC
Cuts at: 1545 1545
Size: 4433
Bme1580I G_kGCm'C
Cuts at: 899 2058 2151 3275 899
Size: 1159 93 1124 2057
Fragments arranged by size:
2057 1159 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1645 1726 2061 4205 1645
Size: 81 335 2144 1873
Fragments arranged by size:
2144 1873 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2800 2800
Size: 4433
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 161
Size: 4433
Bpu10I CC'TnA_GC
Cuts at: 837 837
Size: 4433
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 870 1609 2488 3202 3500 870
Size: 739 879 714 298 1803
Fragments arranged by size:
1803 879 739 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 646 4137 646
Size: 3491 942
BsaAI yAC'GTr
Cuts at: 1786 1786
Size: 4433
BsaBI GATnn'nnATC
Cuts at: 6 461 1947 3984 6
Size: 455 1486 2037 455
Fragments arranged by size:
2037 1486 455 455
BsaHI Gr'CG_yC
Cuts at: 971 1864 2112 2814 4310 971
Size: 893 248 702 1496 1094
Fragments arranged by size:
1496 1094 893 702 248
BsaWI w'CCGG_w
Cuts at: 1622 2143 2821 3232 3379 3653 1622
Size: 521 678 411 147 274 2402
Fragments arranged by size:
2402 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 400 400
Size: 4433
BseYI C'CCAG_C
Cuts at: 1858 2616 3281 3689 1858
Size: 758 665 408 2602
Fragments arranged by size:
2602 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1975 2021 3251 104
Size: 1871 46 1230 1286
Fragments arranged by size:
1871 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1447 2225 2415 3275 1447
Size: 778 190 860 2605
Fragments arranged by size:
2605 860 778 190
BsiWI C'GTAC_G
Cuts at: 906 906
Size: 4433
BsmI GAATG_Cn'
Cuts at: 509 509
Size: 4433
Bsp1286I G_dGCh'C
Cuts at: 493 899 1447 2058 2151 2225 2415 2477 3275
Size: 406 548 611 93 74 190 62 798
Cuts at: 3275 493
Size: 1651
Fragments arranged by size:
1651 798 611 548 406 190 93 74
62
BspHI T'CATG_A
Cuts at: 2865 2865
Size: 4433
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1483 1697 1999 2380 1483
Size: 214 302 381 3536
Fragments arranged by size:
3536 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2725 2779 4263 4428 50
Size: 2675 54 1484 165 55
Fragments arranged by size:
2675 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2345 2345
Size: 4433
BsrFI r'CCGG_y
Cuts at: 281 2431 2612 281
Size: 2150 181 2102
Fragments arranged by size:
2150 2102 181
BsrGI T'GTAC_A
Cuts at: 1303 1303
Size: 4433
BssSI C'ACGA_G
Cuts at: 2704 3412 2704
Size: 708 3725
BstAPI GCAn_nnn'nTGC
Cuts at: 1810 1810
Size: 4433
BstBI TT'CG_AA
Cuts at: 2794 2794
Size: 4433
BstXI CCAn_nnnn'nTGG
Cuts at: 1057 1057
Size: 4433
BstYI r'GATC_y
Cuts at: 1 531 609 1119 1458 1925 1948 2283 2529
Size: 530 78 510 339 467 23 335 246
Cuts at: 2529 2835 2847 2933 3985 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 530 510 467 449 339 335 306
246 86 78 23 12
Bsu36I CC'TnA_GG
Cuts at: 764 1412 764
Size: 648 3785
BtgI C'CryG_G
Cuts at: 107 107
Size: 4433
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2383 2564 2727 2383
Size: 181 163 4089
Fragments arranged by size:
4089 181 163
BtsI GCAGTG_nn'
Cuts at: 1676 1676
Size: 4433
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 185 220 987 1022 185
Size: 35 767 35 3596
Fragments arranged by size:
3596 767 35 35
DraI TTT'AAA
Cuts at: 1432 1432
Size: 4433
DraIII CAC_nnn'GTG
Cuts at: 278 573 278
Size: 295 4138
DrdI GACnn_nn'nnGTC
Cuts at: 426 2139 3483 426
Size: 1713 1344 1376
Fragments arranged by size:
1713 1376 1344
EaeI y'GGCC_r
Cuts at: 1094 2018 2192 2583 2610 3955 1094
Size: 924 174 391 27 1345 1572
Fragments arranged by size:
1572 1345 924 391 174 27
EagI C'GGCC_G
Cuts at: 2018 2018
Size: 4433
EarI CTCTTCn'nnn_
Cuts at: 440 737 2456 2666 440
Size: 297 1719 210 2207
Fragments arranged by size:
2207 1719 297 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2641 3383 3529 4243 2641
Size: 742 146 714 2831
Fragments arranged by size:
2831 742 714 146
EcoICRI GAG'CTC
Cuts at: 1445 1445
Size: 4433
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 316 950 2258 2690 3043 3705 161
Size: 155 634 1308 432 353 662 889
Fragments arranged by size:
1308 889 662 634 432 353 155
EcoNI CCTnn'n_nnAGG
Cuts at: 794 794
Size: 4433
EcoO109I rG'GnC_Cy
Cuts at: 419 642 1572 419
Size: 223 930 3280
Fragments arranged by size:
3280 930 223
EcoRI G'AATT_C
Cuts at: 337 1437 337
Size: 1100 3333
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 337 1215 2452 337
Size: 878 1237 2318
Fragments arranged by size:
2318 1237 878
FspI TGC'GCA
Cuts at: 2214 2214
Size: 4433
HaeII r_GCGC'y
Cuts at: 1867 2115 3345 4116 1867
Size: 248 1230 771 2184
Fragments arranged by size:
2184 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 114 146 379 411 1261 1293 2275 2305 2307
Size: 32 233 32 850 32 982 30 2
Cuts at: 2307 2337 114
Size: 30 2210
Fragments arranged by size:
2210 982 850 233 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1472 1472
Size: 4433
KasI G'GCGC_C
Cuts at: 1863 2111 1863
Size: 248 4185
KpnI G_GTAC'C
Cuts at: 1453 1453
Size: 4433
MluI A'CGCG_T
Cuts at: 3866 3866
Size: 4433
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 656 1016 1034 3192 3376 656
Size: 360 18 2158 184 1713
Fragments arranged by size:
2158 1713 360 184 18
MscI TGG'CCA
Cuts at: 1096 2194 1096
Size: 1098 3335
MslI CAynn'nnrTG
Cuts at: 625 2422 2549 625
Size: 1797 127 2509
Fragments arranged by size:
2509 1797 127
MspA1I CmG'CkG
Cuts at: 1544 1858 2218 3002 3247 1544
Size: 314 360 784 245 2730
Fragments arranged by size:
2730 784 360 314 245
NaeI GCC'GGC
Cuts at: 2614 2614
Size: 4433
NarI GG'CG_CC
Cuts at: 1864 2112 1864
Size: 248 4185
NcoI C'CATG_G
Cuts at: 107 107
Size: 4433
NgoMIV G'CCGG_C
Cuts at: 2612 2612
Size: 4433
NspI r_CATG'y
Cuts at: 580 940 1486 1986 3589 580
Size: 360 546 500 1603 1424
Fragments arranged by size:
1603 1424 546 500 360
PciI A'CATG_T
Cuts at: 3585 3585
Size: 4433
PfoI T'CCnGG_A
Cuts at: 138 138
Size: 4433
PmeI GTTT'AAAC
Cuts at: 1432 1432
Size: 4433
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2879 2911 2879
Size: 32 4401
PpuMI rG'GwC_Cy
Cuts at: 419 419
Size: 4433
PsiI TTA'TAA
Cuts at: 1391 1391
Size: 4433
PspOMI G'GGCC_C
Cuts at: 895 895
Size: 4433
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 176 208 176
Size: 32 4401
PstI C_TGCA'G
Cuts at: 1480 2165 1480
Size: 685 3748
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4433
PvuII CAG'CTG
Cuts at: 1858 2218 1858
Size: 360 4073
RsrII CG'GwC_CG
Cuts at: 2628 2628
Size: 4433
SacI G_AGCT'C
Cuts at: 1447 1447
Size: 4433
SalI G'TCGA_C
Cuts at: 1470 1470
Size: 4433
SanDI GG'GwC_CC
Cuts at: 419 419
Size: 4433
SapI GCTCTTCn'nnn_
Cuts at: 2456 2666 2456
Size: 210 4223
SbfI CC_TGCA'GG
Cuts at: 1480 1480
Size: 4433
ScaI AGT'ACT
Cuts at: 3950 3950
Size: 4433
SfcI C'TryA_G
Cuts at: 33 1476 2161 3129 3320 33
Size: 1443 685 968 191 1146
Fragments arranged by size:
1443 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1865 2113 1865
Size: 248 4185
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4433
SmaI CCC'GGG
Cuts at: 1455 1455
Size: 4433
SmlI C'TyrA_G
Cuts at: 849 1326 1588 2503 3217 3479 849
Size: 477 262 915 714 262 1803
Fragments arranged by size:
1803 915 714 477 262 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4433
SphI G_CATG'C
Cuts at: 580 1486 1986 580
Size: 906 500 3027
Fragments arranged by size:
3027 906 500
SspI AAT'ATT
Cuts at: 736 736
Size: 4433
StyI C'CwwG_G
Cuts at: 107 440 885 1567 1921 107
Size: 333 445 682 354 2619
Fragments arranged by size:
2619 682 445 354 333
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 543 739 2016 2149 2608 2817 543
Size: 196 1277 133 459 209 2159
Fragments arranged by size:
2159 1277 459 209 196 133
TatI w'GTAC_w
Cuts at: 1303 3948 1303
Size: 2645 1788
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3950 3982 3950
Size: 32 4401
Tth111I GACn'n_nGTC
Cuts at: 2230 2230
Size: 4433
XbaI T'CTAG_A
Cuts at: 1464 1464
Size: 4433
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1928 1928
Size: 4433
XmaI C'CCGG_G
Cuts at: 1453 1453
Size: 4433
XmnI GAAnn'nnTTC
Cuts at: 872 1382 872
Size: 510 3923
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PpuMI PsiI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AlfI AloI
AscI BaeI BcgI BfrBI BglI BmgBI BmtI BsaXI
BsgI BsmBI BspEI BssHII BstEII BstZ17I ClaI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PshAI PspXI
SacII SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI