Restriction map for
pF1KB3428
(Circular) (Six-base) MAPSORT of: pF1KB3428.seq Check: 20 from: 1 to: 4382
pF1KB3428 4382 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 1420 1420
Size: 4382
Acc65I G'GTAC_C
Cuts at: 1398 1398
Size: 4382
AclI AA'CG_TT
Cuts at: 4222 4222
Size: 4382
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 664 913 1345 2207 2639 3386 3654 664
Size: 249 432 862 432 747 268 1392
Fragments arranged by size:
1392 862 747 432 432 268 249
AflIII A'CryG_T
Cuts at: 1734 2838 3815 1734
Size: 1104 977 2301
Fragments arranged by size:
2301 1104 977
AgeI A'CCGG_T
Cuts at: 2770 2770
Size: 4382
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 170 797 170
Size: 627 3755
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 156 188 156
Size: 32 4350
AlwNI CAG_nnn'CTG
Cuts at: 615 1687 3254 615
Size: 1072 1567 1743
Fragments arranged by size:
1743 1567 1072
ApaLI G'TGCA_C
Cuts at: 3152 3152
Size: 4382
ApoI r'AATT_y
Cuts at: 1386 3735 3991 1386
Size: 2349 256 1777
Fragments arranged by size:
2349 1777 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4382
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
AvaI C'yCGr_G
Cuts at: 272 1402 272
Size: 1130 3252
BamHI G'GATC_C
Cuts at: 1407 1407
Size: 4382
BanI G'GyrC_C
Cuts at: 597 1398 1812 2060 2095 597
Size: 801 414 248 35 2884
Fragments arranged by size:
2884 801 414 248 35
BanII G_rGCy'C
Cuts at: 358 1396 2426 358
Size: 1038 1030 2314
Fragments arranged by size:
2314 1038 1030
BbeI G_GCGC'C
Cuts at: 1816 2064 1816
Size: 248 4134
BbsI GAAGACnn'nnnn_
Cuts at: 319 1106 1159 319
Size: 787 53 3542
Fragments arranged by size:
3542 787 53
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1329 1363 1329
Size: 34 4348
BciVI GTATCCnnnnn_n'
Cuts at: 2280 3041 4382 2280
Size: 761 1341 2280
Fragments arranged by size:
2280 1341 761
BclI T'GATC_A
Cuts at: 1902 1902
Size: 4382
BglII A'GATC_T
Cuts at: 1897 3934 1897
Size: 2037 2345
BlpI GC'TnA_GC
Cuts at: 1042 1494 1042
Size: 452 3930
Bme1580I G_kGCm'C
Cuts at: 2007 2100 3156 2007
Size: 93 1056 3233
Fragments arranged by size:
3233 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1594 1675 2010 4154 1594
Size: 81 335 2144 1822
Fragments arranged by size:
2144 1822 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1335 2749 1335
Size: 1414 2968
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 937 1044 460
Size: 477 107 3798
Fragments arranged by size:
3798 477 107
Bpu10I CC'TnA_GC
Cuts at: 319 319
Size: 4382
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1558 2437 2929 3227 1558
Size: 879 492 298 2713
Fragments arranged by size:
2713 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4086 4086
Size: 4382
BsaAI yAC'GTr
Cuts at: 1735 1735
Size: 4382
BsaBI GATnn'nnATC
Cuts at: 6 1896 3933 6
Size: 1890 2037 455
Fragments arranged by size:
2037 1890 455
BsaHI Gr'CG_yC
Cuts at: 1813 2061 2763 4259 1813
Size: 248 702 1496 1936
Fragments arranged by size:
1936 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1571 2092 2770 3044 3191 3602 1571
Size: 521 678 274 147 411 2351
Fragments arranged by size:
2351 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 895 925 941 971 895
Size: 30 16 30 4306
Fragments arranged by size:
4306 30 30 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 586 672 586
Size: 86 4296
BseYI C'CCAG_C
Cuts at: 770 1807 2565 3142 3638 770
Size: 1037 758 577 496 1514
Fragments arranged by size:
1514 1037 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 454 814 166
Size: 288 360 3734
Fragments arranged by size:
3734 360 288
BsiEI CG_ry'CG
Cuts at: 104 1924 1970 3178 104
Size: 1820 46 1208 1308
Fragments arranged by size:
1820 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 410 1295 1396 2174 2364 3156 410
Size: 885 101 778 190 792 1636
Fragments arranged by size:
1636 885 792 778 190 101
Bsp1286I G_dGCh'C
Cuts at: 358 410 1295 1396 2007 2100 2174 2364 2426
Size: 52 885 101 611 93 74 190 62
Cuts at: 2426 3156 358
Size: 730 1584
Fragments arranged by size:
1584 885 730 611 190 101 93 74
62 52
BspEI T'CCGG_A
Cuts at: 3602 3602
Size: 4382
BspHI T'CATG_A
Cuts at: 3558 3558
Size: 4382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 395 890 1355 1432 1646 1948 2329 395
Size: 495 465 77 214 302 381 2448
Fragments arranged by size:
2448 495 465 381 302 214 77
BsrBI CCG'CTC
Cuts at: 50 115 2674 2728 4212 4377 50
Size: 65 2559 54 1484 165 55
Fragments arranged by size:
2559 1484 165 65 55 54
BsrDI GCAATG_nn'
Cuts at: 524 2294 524
Size: 1770 2612
BsrFI r'CCGG_y
Cuts at: 2380 2561 2770 2380
Size: 181 209 3992
Fragments arranged by size:
3992 209 181
BssSI C'ACGA_G
Cuts at: 2653 3011 2653
Size: 358 4024
BstAPI GCAn_nnn'nTGC
Cuts at: 1759 1759
Size: 4382
BstBI TT'CG_AA
Cuts at: 2743 2743
Size: 4382
BstXI CCAn_nnnn'nTGG
Cuts at: 915 915
Size: 4382
BstYI r'GATC_y
Cuts at: 1 287 764 1407 1874 1897 2232 2478 3490
Size: 286 477 643 467 23 335 246 1012
Cuts at: 3490 3576 3588 3934 1
Size: 86 12 346 449
Fragments arranged by size:
1012 643 477 467 449 346 335 286
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4382
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2332 2513 2676 2332
Size: 181 163 4038
Fragments arranged by size:
4038 181 163
BtsI GCAGTG_nn'
Cuts at: 213 1625 213
Size: 1412 2970
DraI TTT'AAA
Cuts at: 1381 1381
Size: 4382
DrdI GACnn_nn'nnGTC
Cuts at: 2088 2946 2088
Size: 858 3524
EaeI y'GGCC_r
Cuts at: 110 200 521 562 1967 2141 2532 2559 3904
Size: 90 321 41 1405 174 391 27 1345
Cuts at: 3904 110
Size: 588
Fragments arranged by size:
1405 1345 588 391 321 174 90 41
27
EagI C'GGCC_G
Cuts at: 1967 1967
Size: 4382
EarI CTCTTCn'nnn_
Cuts at: 403 709 1300 2405 2615 403
Size: 306 591 1105 210 2170
Fragments arranged by size:
2170 1105 591 306 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2590 2900 3046 4192 2590
Size: 310 146 1146 2780
Fragments arranged by size:
2780 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1394 1394
Size: 4382
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 664 913 937 1044 1345 2207 2639 3386
Size: 204 249 24 107 301 862 432 747
Cuts at: 3386 3654 460
Size: 268 1188
Fragments arranged by size:
1188 862 747 432 301 268 249 204
107 24
EcoO109I rG'GnC_Cy
Cuts at: 1161 1344 1521 1161
Size: 183 177 4022
Fragments arranged by size:
4022 183 177
EcoRI G'AATT_C
Cuts at: 1386 1386
Size: 4382
EcoRV GAT'ATC
Cuts at: 852 852
Size: 4382
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 390 2401 390
Size: 2011 2371
FspI TGC'GCA
Cuts at: 2163 2163
Size: 4382
HaeII r_GCGC'y
Cuts at: 1816 2064 3086 4065 1816
Size: 248 1022 979 2133
Fragments arranged by size:
2133 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 249 281 2224 2254 2256 2286 249
Size: 32 1943 30 2 30 2345
Fragments arranged by size:
2345 1943 32 30 30 2
HincII GTy'rAC
Cuts at: 1421 1421
Size: 4382
HindIII A'AGCT_T
Cuts at: 1327 1327
Size: 4382
KasI G'GCGC_C
Cuts at: 1812 2060 1812
Size: 248 4134
KpnI G_GTAC'C
Cuts at: 1402 1402
Size: 4382
MluI A'CGCG_T
Cuts at: 3815 3815
Size: 4382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 200 3053 3237 200
Size: 2853 184 1345
Fragments arranged by size:
2853 1345 184
MscI TGG'CCA
Cuts at: 202 523 2143 202
Size: 321 1620 2441
Fragments arranged by size:
2441 1620 321
MslI CAynn'nnrTG
Cuts at: 1018 1147 2371 2498 1018
Size: 129 1224 127 2902
Fragments arranged by size:
2902 1224 129 127
MspA1I CmG'CkG
Cuts at: 828 1493 1807 2167 3180 3425 828
Size: 665 314 360 1013 245 1785
Fragments arranged by size:
1785 1013 665 360 314 245
NaeI GCC'GGC
Cuts at: 2563 2563
Size: 4382
NarI GG'CG_CC
Cuts at: 1813 2061 1813
Size: 248 4134
NcoI C'CATG_G
Cuts at: 107 107
Size: 4382
NgoMIV G'CCGG_C
Cuts at: 2561 2561
Size: 4382
NspI r_CATG'y
Cuts at: 1435 1935 2842 1435
Size: 500 907 2975
Fragments arranged by size:
2975 907 500
PciI A'CATG_T
Cuts at: 2838 2838
Size: 4382
PflMI CCAn_nnn'nTGG
Cuts at: 675 675
Size: 4382
PmeI GTTT'AAAC
Cuts at: 1381 1381
Size: 4382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 595 627 3521 3553 595
Size: 32 2894 32 1424
Fragments arranged by size:
2894 1424 32 32
PpuMI rG'GwC_Cy
Cuts at: 1161 1344 1161
Size: 183 4199
PshAI GACnn'nnGTC
Cuts at: 1159 1159
Size: 4382
PstI C_TGCA'G
Cuts at: 692 1429 2114 692
Size: 737 685 2960
Fragments arranged by size:
2960 737 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4382
PvuII CAG'CTG
Cuts at: 828 1807 2167 828
Size: 979 360 3043
Fragments arranged by size:
3043 979 360
RsrII CG'GwC_CG
Cuts at: 2577 2577
Size: 4382
SacI G_AGCT'C
Cuts at: 1396 1396
Size: 4382
SalI G'TCGA_C
Cuts at: 1419 1419
Size: 4382
SapI GCTCTTCn'nnn_
Cuts at: 1300 2405 2615 1300
Size: 1105 210 3067
Fragments arranged by size:
3067 1105 210
SbfI CC_TGCA'GG
Cuts at: 1429 1429
Size: 4382
ScaI AGT'ACT
Cuts at: 3899 3899
Size: 4382
SfcI C'TryA_G
Cuts at: 33 688 823 1425 2110 3103 3294 33
Size: 655 135 602 685 993 191 1121
Fragments arranged by size:
1121 993 685 655 602 191 135
SfoI GGC'GCC
Cuts at: 1814 2062 1814
Size: 248 4134
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
SmaI CCC'GGG
Cuts at: 1404 1404
Size: 4382
SmlI C'TyrA_G
Cuts at: 1537 2452 2944 3206 1537
Size: 915 492 262 2713
Fragments arranged by size:
2713 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4382
SphI G_CATG'C
Cuts at: 1435 1935 1435
Size: 500 3882
StyI C'CwwG_G
Cuts at: 107 1516 1870 107
Size: 1409 354 2619
Fragments arranged by size:
2619 1409 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1020 1266 1965 2098 2557 2766 1020
Size: 246 699 133 459 209 2636
Fragments arranged by size:
2636 699 459 246 209 133
TatI w'GTAC_w
Cuts at: 3897 3897
Size: 4382
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 941 973 3899 3931 941
Size: 32 2926 32 1392
Fragments arranged by size:
2926 1392 32 32
Tth111I GACn'n_nGTC
Cuts at: 2179 2179
Size: 4382
XbaI T'CTAG_A
Cuts at: 1413 1413
Size: 4382
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 183 550 1877 183
Size: 367 1327 2688
Fragments arranged by size:
2688 1327 367
XmaI C'CCGG_G
Cuts at: 1402 1402
Size: 4382
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI
PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI