Restriction map for
pF1KB3432
(Circular) (Six-base) MAPSORT of: pF1KB3432.seq Check: 526 from: 1 to: 3455
pF1KB3432 3455 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 493 493
Size: 3455
Acc65I G'GTAC_C
Cuts at: 471 471
Size: 3455
AclI AA'CG_TT
Cuts at: 3295 3295
Size: 3455
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1280 1712 2459 2727 1280
Size: 432 747 268 2008
Fragments arranged by size:
2008 747 432 268
AflIII A'CryG_T
Cuts at: 807 1911 2888 807
Size: 1104 977 1374
Fragments arranged by size:
1374 1104 977
AgeI A'CCGG_T
Cuts at: 1843 1843
Size: 3455
AlwNI CAG_nnn'CTG
Cuts at: 322 760 2327 322
Size: 438 1567 1450
Fragments arranged by size:
1567 1450 438
ApaLI G'TGCA_C
Cuts at: 2225 2225
Size: 3455
ApoI r'AATT_y
Cuts at: 459 2808 3064 459
Size: 2349 256 850
Fragments arranged by size:
2349 850 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3455
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3455
AvaI C'yCGr_G
Cuts at: 475 475
Size: 3455
BamHI G'GATC_C
Cuts at: 480 480
Size: 3455
BanI G'GyrC_C
Cuts at: 471 885 1133 1168 471
Size: 414 248 35 2758
Fragments arranged by size:
2758 414 248 35
BanII G_rGCy'C
Cuts at: 128 469 1499 128
Size: 341 1030 2084
Fragments arranged by size:
2084 1030 341
BbeI G_GCGC'C
Cuts at: 889 1137 889
Size: 248 3207
BbvCI CC'TCA_GC
Cuts at: 374 374
Size: 3455
BciVI GTATCCnnnnn_n'
Cuts at: 1353 2114 3455 1353
Size: 761 1341 1353
Fragments arranged by size:
1353 1341 761
BclI T'GATC_A
Cuts at: 975 975
Size: 3455
BglI GCCn_nnn'nGGC
Cuts at: 234 234
Size: 3455
BglII A'GATC_T
Cuts at: 970 3007 970
Size: 2037 1418
BlpI GC'TnA_GC
Cuts at: 567 567
Size: 3455
Bme1580I G_kGCm'C
Cuts at: 1080 1173 2229 1080
Size: 93 1056 2306
Fragments arranged by size:
2306 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 667 748 1083 3227 667
Size: 81 335 2144 895
Fragments arranged by size:
2144 895 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1822 1822
Size: 3455
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 299 353 299
Size: 54 3401
Bpu10I CC'TnA_GC
Cuts at: 374 374
Size: 3455
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 631 1510 2002 2300 631
Size: 879 492 298 1786
Fragments arranged by size:
1786 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3159 3159
Size: 3455
BsaAI yAC'GTr
Cuts at: 808 808
Size: 3455
BsaBI GATnn'nnATC
Cuts at: 6 969 3006 6
Size: 963 2037 455
Fragments arranged by size:
2037 963 455
BsaHI Gr'CG_yC
Cuts at: 886 1134 1836 3332 886
Size: 248 702 1496 1009
Fragments arranged by size:
1496 1009 702 248
BsaWI w'CCGG_w
Cuts at: 644 1165 1843 2117 2264 2675 644
Size: 521 678 274 147 411 1424
Fragments arranged by size:
1424 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 391 427 391
Size: 36 3419
BseYI C'CCAG_C
Cuts at: 880 1638 2215 2711 880
Size: 758 577 496 1624
Fragments arranged by size:
1624 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 314 139
Size: 175 3280
BsiEI CG_ry'CG
Cuts at: 104 997 1043 2251 104
Size: 893 46 1208 1308
Fragments arranged by size:
1308 1208 893 46
BsiHKAI G_wGCw'C
Cuts at: 128 469 1247 1437 2229 128
Size: 341 778 190 792 1354
Fragments arranged by size:
1354 792 778 341 190
Bsp1286I G_dGCh'C
Cuts at: 128 469 1080 1173 1247 1437 1499 2229 128
Size: 341 611 93 74 190 62 730 1354
Fragments arranged by size:
1354 730 611 341 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2675 2675
Size: 3455
BspHI T'CATG_A
Cuts at: 2631 2631
Size: 3455
BspMI ACCTGCnnnn'nnnn_
Cuts at: 318 505 719 1021 1402 318
Size: 187 214 302 381 2371
Fragments arranged by size:
2371 381 302 214 187
BsrBI CCG'CTC
Cuts at: 50 1747 1801 3285 3450 50
Size: 1697 54 1484 165 55
Fragments arranged by size:
1697 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 297 1367 297
Size: 1070 2385
BsrFI r'CCGG_y
Cuts at: 211 301 1453 1634 1843 211
Size: 90 1152 181 209 1823
Fragments arranged by size:
1823 1152 209 181 90
BssSI C'ACGA_G
Cuts at: 1726 2084 1726
Size: 358 3097
BstAPI GCAn_nnn'nTGC
Cuts at: 325 832 325
Size: 507 2948
BstBI TT'CG_AA
Cuts at: 1816 1816
Size: 3455
BstYI r'GATC_y
Cuts at: 1 480 947 970 1305 1551 2563 2649 2661
Size: 479 467 23 335 246 1012 86 12
Cuts at: 2661 3007 1
Size: 346 449
Fragments arranged by size:
1012 479 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3455
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1405 1586 1749 1405
Size: 181 163 3111
Fragments arranged by size:
3111 181 163
BtsI GCAGTG_nn'
Cuts at: 356 698 356
Size: 342 3113
DraI TTT'AAA
Cuts at: 454 454
Size: 3455
DrdI GACnn_nn'nnGTC
Cuts at: 1161 2019 1161
Size: 858 2597
EaeI y'GGCC_r
Cuts at: 260 1040 1214 1605 1632 2977 260
Size: 780 174 391 27 1345 738
Fragments arranged by size:
1345 780 738 391 174 27
EagI C'GGCC_G
Cuts at: 1040 1040
Size: 3455
EarI CTCTTCn'nnn_
Cuts at: 1478 1688 1478
Size: 210 3245
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1663 1973 2119 3265 1663
Size: 310 146 1146 1853
Fragments arranged by size:
1853 1146 310 146
EcoICRI GAG'CTC
Cuts at: 126 467 126
Size: 341 3114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 299 353 1280 1712 2459 2727 299
Size: 54 927 432 747 268 1027
Fragments arranged by size:
1027 927 747 432 268 54
EcoO109I rG'GnC_Cy
Cuts at: 255 343 594 255
Size: 88 251 3116
Fragments arranged by size:
3116 251 88
EcoRI G'AATT_C
Cuts at: 459 459
Size: 3455
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1474 1474
Size: 3455
FspI TGC'GCA
Cuts at: 1236 1236
Size: 3455
HaeII r_GCGC'y
Cuts at: 889 1137 2159 3138 889
Size: 248 1022 979 1206
Fragments arranged by size:
1206 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1297 1327 1329 1359 1297
Size: 30 2 30 3393
Fragments arranged by size:
3393 30 30 2
HincII GTy'rAC
Cuts at: 270 494 270
Size: 224 3231
KasI G'GCGC_C
Cuts at: 885 1133 885
Size: 248 3207
KpnI G_GTAC'C
Cuts at: 475 475
Size: 3455
MluI A'CGCG_T
Cuts at: 2888 2888
Size: 3455
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2126 2310 2126
Size: 184 3271
MscI TGG'CCA
Cuts at: 262 1216 262
Size: 954 2501
MslI CAynn'nnrTG
Cuts at: 161 1444 1571 161
Size: 1283 127 2045
Fragments arranged by size:
2045 1283 127
MspA1I CmG'CkG
Cuts at: 322 373 566 880 1240 2253 2498 322
Size: 51 193 314 360 1013 245 1279
Fragments arranged by size:
1279 1013 360 314 245 193 51
NaeI GCC'GGC
Cuts at: 1636 1636
Size: 3455
NarI GG'CG_CC
Cuts at: 886 1134 886
Size: 248 3207
NcoI C'CATG_G
Cuts at: 107 107
Size: 3455
NgoMIV G'CCGG_C
Cuts at: 1634 1634
Size: 3455
NspI r_CATG'y
Cuts at: 508 1008 1915 508
Size: 500 907 2048
Fragments arranged by size:
2048 907 500
PciI A'CATG_T
Cuts at: 1911 1911
Size: 3455
PflMI CCAn_nnn'nTGG
Cuts at: 322 322
Size: 3455
PmeI GTTT'AAAC
Cuts at: 454 454
Size: 3455
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2594 2626 2594
Size: 32 3423
PpuMI rG'GwC_Cy
Cuts at: 343 343
Size: 3455
PstI C_TGCA'G
Cuts at: 502 1187 502
Size: 685 2770
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3455
PvuII CAG'CTG
Cuts at: 322 373 880 1240 322
Size: 51 507 360 2537
Fragments arranged by size:
2537 507 360 51
RsrII CG'GwC_CG
Cuts at: 1650 1650
Size: 3455
SacI G_AGCT'C
Cuts at: 128 469 128
Size: 341 3114
SalI G'TCGA_C
Cuts at: 492 492
Size: 3455
SapI GCTCTTCn'nnn_
Cuts at: 1478 1688 1478
Size: 210 3245
SbfI CC_TGCA'GG
Cuts at: 502 502
Size: 3455
ScaI AGT'ACT
Cuts at: 2972 2972
Size: 3455
SfcI C'TryA_G
Cuts at: 33 498 1183 2176 2367 33
Size: 465 685 993 191 1121
Fragments arranged by size:
1121 993 685 465 191
SfoI GGC'GCC
Cuts at: 887 1135 887
Size: 248 3207
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3455
SmaI CCC'GGG
Cuts at: 477 477
Size: 3455
SmlI C'TyrA_G
Cuts at: 610 1525 2017 2279 610
Size: 915 492 262 1786
Fragments arranged by size:
1786 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3455
SphI G_CATG'C
Cuts at: 508 1008 508
Size: 500 2955
StyI C'CwwG_G
Cuts at: 107 589 943 107
Size: 482 354 2619
Fragments arranged by size:
2619 482 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1038 1171 1630 1839 1038
Size: 133 459 209 2654
Fragments arranged by size:
2654 459 209 133
TatI w'GTAC_w
Cuts at: 2970 2970
Size: 3455
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2972 3004 2972
Size: 32 3423
Tth111I GACn'n_nGTC
Cuts at: 1252 1252
Size: 3455
XbaI T'CTAG_A
Cuts at: 486 486
Size: 3455
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 950 950
Size: 3455
XmaI C'CCGG_G
Cuts at: 475 475
Size: 3455
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BmgBI
BmtI BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI