Restriction map for
pF1KB3434
(Circular) (Six-base) MAPSORT of: pF1KB3434.seq Check: 5606 from: 1 to: 4082
pF1KB3434 4082 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 1120 1120
Size: 4082
Acc65I G'GTAC_C
Cuts at: 782 1098 782
Size: 316 3766
AclI AA'CG_TT
Cuts at: 3922 3922
Size: 4082
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 661 1907 2339 3086 3354 271
Size: 390 1246 432 747 268 999
Fragments arranged by size:
1246 999 747 432 390 268
AflIII A'CryG_T
Cuts at: 497 1434 2538 3515 497
Size: 937 1104 977 1064
Fragments arranged by size:
1104 1064 977 937
AgeI A'CCGG_T
Cuts at: 2470 2470
Size: 4082
AleI CACnn'nnGTG
Cuts at: 511 511
Size: 4082
AlwNI CAG_nnn'CTG
Cuts at: 534 1387 2954 534
Size: 853 1567 1662
Fragments arranged by size:
1662 1567 853
ApaLI G'TGCA_C
Cuts at: 717 832 2852 717
Size: 115 2020 1947
Fragments arranged by size:
2020 1947 115
ApoI r'AATT_y
Cuts at: 1086 3435 3691 1086
Size: 2349 256 1477
Fragments arranged by size:
2349 1477 256
AscI GG'CGCG_CC
Cuts at: 843 843
Size: 4082
AseI AT'TA_AT
Cuts at: 20 20
Size: 4082
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4082
AvaI C'yCGr_G
Cuts at: 173 916 985 1102 173
Size: 743 69 117 3153
Fragments arranged by size:
3153 743 117 69
AvrII C'CTAG_G
Cuts at: 270 270
Size: 4082
BamHI G'GATC_C
Cuts at: 1107 1107
Size: 4082
BanI G'GyrC_C
Cuts at: 211 782 925 1098 1512 1760 1795 211
Size: 571 143 173 414 248 35 2498
Fragments arranged by size:
2498 571 414 248 173 143 35
BanII G_rGCy'C
Cuts at: 174 923 1096 2126 174
Size: 749 173 1030 2130
Fragments arranged by size:
2130 1030 749 173
BbeI G_GCGC'C
Cuts at: 929 1516 1764 929
Size: 587 248 3247
Fragments arranged by size:
3247 587 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4048
BciVI GTATCCnnnnn_n'
Cuts at: 1980 2741 4082 1980
Size: 761 1341 1980
Fragments arranged by size:
1980 1341 761
BclI T'GATC_A
Cuts at: 1602 1602
Size: 4082
BglI GCCn_nnn'nGGC
Cuts at: 112 455 822 112
Size: 343 367 3372
Fragments arranged by size:
3372 367 343
BglII A'GATC_T
Cuts at: 1597 3634 1597
Size: 2037 2045
BlpI GC'TnA_GC
Cuts at: 955 1194 955
Size: 239 3843
Bme1580I G_kGCm'C
Cuts at: 216 721 836 1707 1800 2856 216
Size: 505 115 871 93 1056 1442
Fragments arranged by size:
1442 1056 871 505 115 93
BmgBI CAC'GTC
Cuts at: 486 486
Size: 4082
BmrI ACTGGGnnnn_n'
Cuts at: 342 1294 1375 1710 3854 342
Size: 952 81 335 2144 570
Fragments arranged by size:
2144 952 570 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2449 2449
Size: 4082
Bpu10I CC'TnA_GC
Cuts at: 695 695
Size: 4082
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1258 2137 2629 2927 1258
Size: 879 492 298 2413
Fragments arranged by size:
2413 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3786 3786
Size: 4082
BsaAI yAC'GTr
Cuts at: 1435 1435
Size: 4082
BsaBI GATnn'nnATC
Cuts at: 6 299 1596 3633 6
Size: 293 1297 2037 455
Fragments arranged by size:
2037 1297 455 293
BsaHI Gr'CG_yC
Cuts at: 926 1047 1513 1761 2463 3959 926
Size: 121 466 248 702 1496 1049
Fragments arranged by size:
1496 1049 702 466 248 121
BsaWI w'CCGG_w
Cuts at: 1271 1792 2470 2744 2891 3302 1271
Size: 521 678 274 147 411 2051
Fragments arranged by size:
2051 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1003 1033 1003
Size: 30 4052
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 235 238 358 379 382 235
Size: 3 120 21 3 3935
Fragments arranged by size:
3935 120 21 3 3
BseYI C'CCAG_C
Cuts at: 146 203 283 400 825 929 934 1507 2265
Size: 57 80 117 425 104 5 573 758
Cuts at: 2265 2842 3338 146
Size: 577 496 890
Fragments arranged by size:
890 758 577 573 496 425 117 104
80 57 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 466 478 457
Size: 9 12 4061
Fragments arranged by size:
4061 12 9
BsiEI CG_ry'CG
Cuts at: 104 624 840 1624 1670 2878 104
Size: 520 216 784 46 1208 1308
Fragments arranged by size:
1308 1208 784 520 216 46
BsiHKAI G_wGCw'C
Cuts at: 620 721 836 923 1096 1874 2064 2856 620
Size: 101 115 87 173 778 190 792 1846
Fragments arranged by size:
1846 792 778 190 173 115 101 87
BsmBI CGTCTCn'nnnn_
Cuts at: 1054 1054
Size: 4082
Bsp1286I G_dGCh'C
Cuts at: 174 216 620 721 836 923 1096 1707 1800
Size: 42 404 101 115 87 173 611 93
Cuts at: 1800 1874 2064 2126 2856 174
Size: 74 190 62 730 1400
Fragments arranged by size:
1400 730 611 404 190 173 115 101
93 87 74 62 42
BspEI T'CCGG_A
Cuts at: 3302 3302
Size: 4082
BspHI T'CATG_A
Cuts at: 3258 3258
Size: 4082
BspMI ACCTGCnnnn'nnnn_
Cuts at: 794 1132 1346 1648 2029 794
Size: 338 214 302 381 2847
Fragments arranged by size:
2847 381 338 302 214
BsrBI CCG'CTC
Cuts at: 50 228 954 2374 2428 3912 4077 50
Size: 178 726 1420 54 1484 165 55
Fragments arranged by size:
1484 1420 726 178 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1994 1994
Size: 4082
BsrFI r'CCGG_y
Cuts at: 454 747 2080 2261 2470 454
Size: 293 1333 181 209 2066
Fragments arranged by size:
2066 1333 293 209 181
BssHII G'CGCG_C
Cuts at: 789 843 789
Size: 54 4028
BssSI C'ACGA_G
Cuts at: 2353 2711 2353
Size: 358 3724
BstAPI GCAn_nnn'nTGC
Cuts at: 480 585 1459 480
Size: 105 874 3103
Fragments arranged by size:
3103 874 105
BstBI TT'CG_AA
Cuts at: 689 866 2443 689
Size: 177 1577 2328
Fragments arranged by size:
2328 1577 177
BstYI r'GATC_y
Cuts at: 1 1107 1574 1597 1932 2178 3190 3276 3288
Size: 1106 467 23 335 246 1012 86 12
Cuts at: 3288 3634 1
Size: 346 449
Fragments arranged by size:
1106 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 419 419
Size: 4082
BtgI C'CryG_G
Cuts at: 165 165
Size: 4082
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 584 886 2032 2213 2376 584
Size: 302 1146 181 163 2290
Fragments arranged by size:
2290 1146 302 181 163
BtsI GCAGTG_nn'
Cuts at: 1325 1325
Size: 4082
DraI TTT'AAA
Cuts at: 1081 1081
Size: 4082
DrdI GACnn_nn'nnGTC
Cuts at: 1788 2646 1788
Size: 858 3224
EaeI y'GGCC_r
Cuts at: 162 452 621 873 1667 1841 2232 2259 3604
Size: 290 169 252 794 174 391 27 1345
Cuts at: 3604 162
Size: 640
Fragments arranged by size:
1345 794 640 391 290 252 174 169
27
EagI C'GGCC_G
Cuts at: 621 1667 621
Size: 1046 3036
EarI CTCTTCn'nnn_
Cuts at: 356 868 2105 2315 356
Size: 512 1237 210 2123
Fragments arranged by size:
2123 1237 512 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2290 2600 2746 3892 2290
Size: 310 146 1146 2480
Fragments arranged by size:
2480 1146 310 146
EcoICRI GAG'CTC
Cuts at: 921 1094 921
Size: 173 3909
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 661 1907 2339 3086 3354 271
Size: 390 1246 432 747 268 999
Fragments arranged by size:
1246 999 747 432 390 268
EcoNI CCTnn'n_nnAGG
Cuts at: 589 589
Size: 4082
EcoO109I rG'GnC_Cy
Cuts at: 240 852 1221 240
Size: 612 369 3101
Fragments arranged by size:
3101 612 369
EcoRI G'AATT_C
Cuts at: 1086 1086
Size: 4082
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2101 2101
Size: 4082
FseI GG_CCGG'CC
Cuts at: 458 458
Size: 4082
FspI TGC'GCA
Cuts at: 1863 1863
Size: 4082
HaeII r_GCGC'y
Cuts at: 775 799 929 941 1516 1764 2786 3765 775
Size: 24 130 12 575 248 1022 979 1092
Fragments arranged by size:
1092 1022 979 575 248 130 24 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1003 1035 1924 1954 1956 1986 1003
Size: 32 889 30 2 30 3099
Fragments arranged by size:
3099 889 32 30 30 2
HincII GTy'rAC
Cuts at: 1121 1121
Size: 4082
KasI G'GCGC_C
Cuts at: 925 1512 1760 925
Size: 587 248 3247
Fragments arranged by size:
3247 587 248
KpnI G_GTAC'C
Cuts at: 786 1102 786
Size: 316 3766
MluI A'CGCG_T
Cuts at: 497 3515 497
Size: 3018 1064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 482 2753 2937 482
Size: 2271 184 1627
Fragments arranged by size:
2271 1627 184
MscI TGG'CCA
Cuts at: 164 1843 164
Size: 1679 2403
MslI CAynn'nnrTG
Cuts at: 511 2071 2198 511
Size: 1560 127 2395
Fragments arranged by size:
2395 1560 127
MspA1I CmG'CkG
Cuts at: 480 543 1044 1193 1507 1867 2880 3125 480
Size: 63 501 149 314 360 1013 245 1437
Fragments arranged by size:
1437 1013 501 360 314 245 149 63
NaeI GCC'GGC
Cuts at: 456 749 2263 456
Size: 293 1514 2275
Fragments arranged by size:
2275 1514 293
NarI GG'CG_CC
Cuts at: 926 1513 1761 926
Size: 587 248 3247
Fragments arranged by size:
3247 587 248
NcoI C'CATG_G
Cuts at: 165 165
Size: 4082
NgoMIV G'CCGG_C
Cuts at: 454 747 2261 454
Size: 293 1514 2275
Fragments arranged by size:
2275 1514 293
NspI r_CATG'y
Cuts at: 1135 1635 2542 1135
Size: 500 907 2675
Fragments arranged by size:
2675 907 500
PasI CC'CwG_GG
Cuts at: 528 528
Size: 4082
PciI A'CATG_T
Cuts at: 2538 2538
Size: 4082
PflMI CCAn_nnn'nTGG
Cuts at: 153 765 153
Size: 612 3470
PmeI GTTT'AAAC
Cuts at: 1081 1081
Size: 4082
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3221 3253 3221
Size: 32 4050
PstI C_TGCA'G
Cuts at: 263 590 1129 1814 263
Size: 327 539 685 2531
Fragments arranged by size:
2531 685 539 327
PvuI CG_AT'CG
Cuts at: 104 840 104
Size: 736 3346
PvuII CAG'CTG
Cuts at: 480 543 1507 1867 480
Size: 63 964 360 2695
Fragments arranged by size:
2695 964 360 63
RsrII CG'GwC_CG
Cuts at: 2277 2277
Size: 4082
SacI G_AGCT'C
Cuts at: 923 1096 923
Size: 173 3909
SalI G'TCGA_C
Cuts at: 1119 1119
Size: 4082
SapI GCTCTTCn'nnn_
Cuts at: 2105 2315 2105
Size: 210 3872
SbfI CC_TGCA'GG
Cuts at: 590 1129 590
Size: 539 3543
ScaI AGT'ACT
Cuts at: 3599 3599
Size: 4082
SfcI C'TryA_G
Cuts at: 33 259 586 1125 1810 2803 2994 33
Size: 226 327 539 685 993 191 1121
Fragments arranged by size:
1121 993 685 539 327 226 191
SfoI GGC'GCC
Cuts at: 927 1514 1762 927
Size: 587 248 3247
Fragments arranged by size:
3247 587 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4082
SmaI CCC'GGG
Cuts at: 1104 1104
Size: 4082
SmlI C'TyrA_G
Cuts at: 985 1237 2152 2644 2906 985
Size: 252 915 492 262 2161
Fragments arranged by size:
2161 915 492 262 252
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4082
SphI G_CATG'C
Cuts at: 1135 1635 1135
Size: 500 3582
StuI AGG'CCT
Cuts at: 470 1006 470
Size: 536 3546
StyI C'CwwG_G
Cuts at: 165 270 1216 1570 165
Size: 105 946 354 2677
Fragments arranged by size:
2677 946 354 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1665 1798 2257 2466 1665
Size: 133 459 209 3281
Fragments arranged by size:
3281 459 209 133
TatI w'GTAC_w
Cuts at: 3597 3597
Size: 4082
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3599 3631 3599
Size: 32 4050
Tth111I GACn'n_nGTC
Cuts at: 427 1879 427
Size: 1452 2630
XbaI T'CTAG_A
Cuts at: 1113 1113
Size: 4082
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 973 1577 973
Size: 604 3478
XhoI C'TCGA_G
Cuts at: 985 985
Size: 4082
XmaI C'CCGG_G
Cuts at: 1102 1102
Size: 4082
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
BaeI BbsI BbvCI BfrBI BmtI BpmI BsiWI BsmI
BsrGI BstEII BstXI BstZ17I ClaI CspCI DraIII EcoRV
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XmnI ZraI