Restriction map for
pF1KB3440
(Circular) (Six-base) MAPSORT of: pF1KB3440.seq Check: 1521 from: 1 to: 3887
pF1KB3440 3887 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 712 712
Size: 3887
AccI GT'mk_AC
Cuts at: 698 925 698
Size: 227 3660
Acc65I G'GTAC_C
Cuts at: 903 903
Size: 3887
AclI AA'CG_TT
Cuts at: 3727 3727
Size: 3887
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1712 2144 2891 3159 1712
Size: 432 747 268 2440
Fragments arranged by size:
2440 747 432 268
AfeI AGC'GCT
Cuts at: 262 262
Size: 3887
AflIII A'CryG_T
Cuts at: 242 1239 2343 3320 242
Size: 997 1104 977 809
Fragments arranged by size:
1104 997 977 809
AgeI A'CCGG_T
Cuts at: 2275 2275
Size: 3887
AleI CACnn'nnGTG
Cuts at: 181 181
Size: 3887
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 143 859 143
Size: 716 3171
AlwNI CAG_nnn'CTG
Cuts at: 660 1192 2759 660
Size: 532 1567 1788
Fragments arranged by size:
1788 1567 532
ApaI G_GGCC'C
Cuts at: 452 512 452
Size: 60 3827
ApaLI G'TGCA_C
Cuts at: 2657 2657
Size: 3887
ApoI r'AATT_y
Cuts at: 891 3240 3496 891
Size: 2349 256 1282
Fragments arranged by size:
2349 1282 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3887
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3887
AvaI C'yCGr_G
Cuts at: 907 907
Size: 3887
AvrII C'CTAG_G
Cuts at: 478 478
Size: 3887
BamHI G'GATC_C
Cuts at: 912 912
Size: 3887
BanI G'GyrC_C
Cuts at: 903 1317 1565 1600 903
Size: 414 248 35 3190
Fragments arranged by size:
3190 414 248 35
BanII G_rGCy'C
Cuts at: 452 512 818 901 1931 452
Size: 60 306 83 1030 2408
Fragments arranged by size:
2408 1030 306 83 60
BbeI G_GCGC'C
Cuts at: 1321 1569 1321
Size: 248 3639
BciVI GTATCCnnnnn_n'
Cuts at: 1785 2546 3887 1785
Size: 761 1341 1785
Fragments arranged by size:
1785 1341 761
BclI T'GATC_A
Cuts at: 1407 1407
Size: 3887
BglII A'GATC_T
Cuts at: 1402 3439 1402
Size: 2037 1850
BlpI GC'TnA_GC
Cuts at: 999 999
Size: 3887
Bme1580I G_kGCm'C
Cuts at: 452 512 1512 1605 2661 452
Size: 60 1000 93 1056 1678
Fragments arranged by size:
1678 1056 1000 93 60
BmgBI CAC'GTC
Cuts at: 318 318
Size: 3887
BmrI ACTGGGnnnn_n'
Cuts at: 1099 1180 1515 3659 1099
Size: 81 335 2144 1327
Fragments arranged by size:
2144 1327 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2254 2254
Size: 3887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 289 412 188
Size: 101 123 3663
Fragments arranged by size:
3663 123 101
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1063 1942 2434 2732 1063
Size: 879 492 298 2218
Fragments arranged by size:
2218 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3591 3591
Size: 3887
BsaAI yAC'GTr
Cuts at: 543 1240 543
Size: 697 3190
BsaBI GATnn'nnATC
Cuts at: 6 1401 3438 6
Size: 1395 2037 455
Fragments arranged by size:
2037 1395 455
BsaHI Gr'CG_yC
Cuts at: 1318 1566 2268 3764 1318
Size: 248 702 1496 1441
Fragments arranged by size:
1496 1441 702 248
BsaWI w'CCGG_w
Cuts at: 1076 1597 2275 2549 2696 3107 1076
Size: 521 678 274 147 411 1856
Fragments arranged by size:
1856 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 307 376 409 867 307
Size: 69 33 458 3327
Fragments arranged by size:
3327 458 69 33
BseYI C'CCAG_C
Cuts at: 345 441 607 683 796 810 1312 2070 2647
Size: 96 166 76 113 14 502 758 577
Cuts at: 2647 3143 345
Size: 496 1089
Fragments arranged by size:
1089 758 577 502 496 166 113 96
76 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 444 444
Size: 3887
BsiEI CG_ry'CG
Cuts at: 104 1429 1475 2683 104
Size: 1325 46 1208 1308
Fragments arranged by size:
1325 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 719 901 1679 1869 2661 719
Size: 182 778 190 792 1945
Fragments arranged by size:
1945 792 778 190 182
BsmI GAATG_Cn'
Cuts at: 241 566 241
Size: 325 3562
Bsp1286I G_dGCh'C
Cuts at: 452 512 719 818 901 1512 1605 1679 1869
Size: 60 207 99 83 611 93 74 190
Cuts at: 1869 1931 2661 452
Size: 62 730 1678
Fragments arranged by size:
1678 730 611 207 190 99 93 83
74 62 60
BspEI T'CCGG_A
Cuts at: 3107 3107
Size: 3887
BspHI T'CATG_A
Cuts at: 3063 3063
Size: 3887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 111 536 712 937 1151 1453 1834 111
Size: 425 176 225 214 302 381 2164
Fragments arranged by size:
2164 425 381 302 225 214 176
BsrBI CCG'CTC
Cuts at: 50 2179 2233 3717 3882 50
Size: 2129 54 1484 165 55
Fragments arranged by size:
2129 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1799 1799
Size: 3887
BsrFI r'CCGG_y
Cuts at: 1885 2066 2275 1885
Size: 181 209 3497
Fragments arranged by size:
3497 209 181
BssSI C'ACGA_G
Cuts at: 2158 2516 2158
Size: 358 3529
BstAPI GCAn_nnn'nTGC
Cuts at: 1264 1264
Size: 3887
BstBI TT'CG_AA
Cuts at: 2248 2248
Size: 3887
BstYI r'GATC_y
Cuts at: 1 656 874 912 1379 1402 1737 1983 2995
Size: 655 218 38 467 23 335 246 1012
Cuts at: 2995 3081 3093 3439 1
Size: 86 12 346 449
Fragments arranged by size:
1012 655 467 449 346 335 246 218
86 38 23 12
BtgI C'CryG_G
Cuts at: 743 743
Size: 3887
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1837 2018 2181 1837
Size: 181 163 3543
Fragments arranged by size:
3543 181 163
BtsI GCAGTG_nn'
Cuts at: 413 1130 413
Size: 717 3170
DraI TTT'AAA
Cuts at: 886 886
Size: 3887
DrdI GACnn_nn'nnGTC
Cuts at: 1593 2451 1593
Size: 858 3029
EaeI y'GGCC_r
Cuts at: 1472 1646 2037 2064 3409 1472
Size: 174 391 27 1345 1950
Fragments arranged by size:
1950 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1472 1472
Size: 3887
EarI CTCTTCn'nnn_
Cuts at: 1910 2120 1910
Size: 210 3677
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2095 2405 2551 3697 2095
Size: 310 146 1146 2285
Fragments arranged by size:
2285 1146 310 146
EcoICRI GAG'CTC
Cuts at: 899 899
Size: 3887
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 289 412 1712 2144 2891 3159 188
Size: 101 123 1300 432 747 268 916
Fragments arranged by size:
1300 916 747 432 268 123 101
EcoNI CCTnn'n_nnAGG
Cuts at: 220 220
Size: 3887
EcoO109I rG'GnC_Cy
Cuts at: 508 509 1026 508
Size: 1 517 3369
Fragments arranged by size:
3369 517 1
EcoRI G'AATT_C
Cuts at: 891 891
Size: 3887
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 528 1906 528
Size: 1378 2509
FspI TGC'GCA
Cuts at: 1668 1668
Size: 3887
HaeII r_GCGC'y
Cuts at: 264 468 1321 1569 2591 3570 264
Size: 204 853 248 1022 979 581
Fragments arranged by size:
1022 979 853 581 248 204
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 132 164 821 853 1729 1759 1761 1791 132
Size: 32 657 32 876 30 2 30 2228
Fragments arranged by size:
2228 876 657 32 32 30 30 2
HincII GTy'rAC
Cuts at: 926 926
Size: 3887
KasI G'GCGC_C
Cuts at: 1317 1565 1317
Size: 248 3639
KpnI G_GTAC'C
Cuts at: 907 907
Size: 3887
MluI A'CGCG_T
Cuts at: 242 3320 242
Size: 3078 809
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2558 2742 2558
Size: 184 3703
MscI TGG'CCA
Cuts at: 1648 1648
Size: 3887
MslI CAynn'nnrTG
Cuts at: 181 841 1876 2003 181
Size: 660 1035 127 2065
Fragments arranged by size:
2065 1035 660 127
MspA1I CmG'CkG
Cuts at: 607 998 1312 1672 2685 2930 607
Size: 391 314 360 1013 245 1564
Fragments arranged by size:
1564 1013 391 360 314 245
NaeI GCC'GGC
Cuts at: 2068 2068
Size: 3887
NarI GG'CG_CC
Cuts at: 1318 1566 1318
Size: 248 3639
NgoMIV G'CCGG_C
Cuts at: 2066 2066
Size: 3887
NspI r_CATG'y
Cuts at: 940 1440 2347 940
Size: 500 907 2480
Fragments arranged by size:
2480 907 500
PciI A'CATG_T
Cuts at: 2343 2343
Size: 3887
PflMI CCAn_nnn'nTGG
Cuts at: 660 660
Size: 3887
PfoI T'CCnGG_A
Cuts at: 266 389 266
Size: 123 3764
PmeI GTTT'AAAC
Cuts at: 886 886
Size: 3887
PmlI CAC'GTG
Cuts at: 543 543
Size: 3887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3026 3058 3026
Size: 32 3855
PspOMI G'GGCC_C
Cuts at: 448 508 448
Size: 60 3827
PstI C_TGCA'G
Cuts at: 122 464 882 934 1619 122
Size: 342 418 52 685 2390
Fragments arranged by size:
2390 685 418 342 52
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3887
PvuII CAG'CTG
Cuts at: 607 1312 1672 607
Size: 705 360 2822
Fragments arranged by size:
2822 705 360
RsrII CG'GwC_CG
Cuts at: 2082 2082
Size: 3887
SacI G_AGCT'C
Cuts at: 901 901
Size: 3887
SalI G'TCGA_C
Cuts at: 924 924
Size: 3887
SapI GCTCTTCn'nnn_
Cuts at: 1910 2120 1910
Size: 210 3677
SbfI CC_TGCA'GG
Cuts at: 934 934
Size: 3887
ScaI AGT'ACT
Cuts at: 367 3404 367
Size: 3037 850
SfcI C'TryA_G
Cuts at: 33 118 460 878 930 1615 2608 2799 33
Size: 85 342 418 52 685 993 191 1121
Fragments arranged by size:
1121 993 685 418 342 191 85 52
SfoI GGC'GCC
Cuts at: 1319 1567 1319
Size: 248 3639
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3887
SmaI CCC'GGG
Cuts at: 909 909
Size: 3887
SmlI C'TyrA_G
Cuts at: 1042 1957 2449 2711 1042
Size: 915 492 262 2218
Fragments arranged by size:
2218 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3887
SphI G_CATG'C
Cuts at: 940 1440 940
Size: 500 3387
StyI C'CwwG_G
Cuts at: 478 1021 1375 478
Size: 543 354 2990
Fragments arranged by size:
2990 543 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1470 1603 2062 2271 1470
Size: 133 459 209 3086
Fragments arranged by size:
3086 459 209 133
TatI w'GTAC_w
Cuts at: 365 3402 365
Size: 3037 850
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 248 280 406 438 3404 3436 248
Size: 32 126 32 2966 32 699
Fragments arranged by size:
2966 699 126 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1684 1684
Size: 3887
XbaI T'CTAG_A
Cuts at: 918 918
Size: 3887
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1382 1382
Size: 3887
XmaI C'CCGG_G
Cuts at: 907 907
Size: 3887
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PfoI PmeI
PmlI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI BaeI BbsI BbvCI
BcgI BfrBI BglI BmtI Bpu10I BsaXI BsiWI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI