Restriction map for
pF1KB3441
(Circular) (Six-base) MAPSORT of: pF1KB3441.seq Check: 2463 from: 1 to: 4133
pF1KB3441 4133 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 862 1012 862
Size: 150 3983
AccI GT'mk_AC
Cuts at: 1171 1171
Size: 4133
Acc65I G'GTAC_C
Cuts at: 1149 1149
Size: 4133
AclI AA'CG_TT
Cuts at: 1127 3973 1127
Size: 2846 1287
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 710 1958 2390 3137 3405 710
Size: 1248 432 747 268 1438
Fragments arranged by size:
1438 1248 747 432 268
AflII C'TTAA_G
Cuts at: 148 148
Size: 4133
AflIII A'CryG_T
Cuts at: 601 1485 2589 3566 601
Size: 884 1104 977 1168
Fragments arranged by size:
1168 1104 977 884
AgeI A'CCGG_T
Cuts at: 2521 2521
Size: 4133
AhdI GACnn_n'nnGTC
Cuts at: 703 703
Size: 4133
AlwNI CAG_nnn'CTG
Cuts at: 1438 3005 1438
Size: 1567 2566
ApaLI G'TGCA_C
Cuts at: 2903 2903
Size: 4133
ApoI r'AATT_y
Cuts at: 1137 3486 3742 1137
Size: 2349 256 1528
Fragments arranged by size:
2349 1528 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4133
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4133
AvaI C'yCGr_G
Cuts at: 1153 1153
Size: 4133
BamHI G'GATC_C
Cuts at: 1158 1158
Size: 4133
BanI G'GyrC_C
Cuts at: 266 953 1149 1563 1811 1846 266
Size: 687 196 414 248 35 2553
Fragments arranged by size:
2553 687 414 248 196 35
BanII G_rGCy'C
Cuts at: 890 1147 2177 890
Size: 257 1030 2846
Fragments arranged by size:
2846 1030 257
BbeI G_GCGC'C
Cuts at: 957 1567 1815 957
Size: 610 248 3275
Fragments arranged by size:
3275 610 248
BciVI GTATCCnnnnn_n'
Cuts at: 2031 2792 4133 2031
Size: 761 1341 2031
Fragments arranged by size:
2031 1341 761
BclI T'GATC_A
Cuts at: 1653 1653
Size: 4133
BfrBI ATG'CAT
Cuts at: 826 826
Size: 4133
BglI GCCn_nnn'nGGC
Cuts at: 1018 1018
Size: 4133
BglII A'GATC_T
Cuts at: 278 1118 1648 3685 278
Size: 840 530 2037 726
Fragments arranged by size:
2037 840 726 530
BlpI GC'TnA_GC
Cuts at: 1245 1245
Size: 4133
Bme1580I G_kGCm'C
Cuts at: 269 301 609 1758 1851 2907 269
Size: 32 308 1149 93 1056 1495
Fragments arranged by size:
1495 1149 1056 308 93 32
BmrI ACTGGGnnnn_n'
Cuts at: 217 303 1345 1426 1761 3905 217
Size: 86 1042 81 335 2144 445
Fragments arranged by size:
2144 1042 445 335 86 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 695 2500 695
Size: 1805 2328
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 291 611 680 291
Size: 320 69 3744
Fragments arranged by size:
3744 320 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1099 1309 2188 2680 2978 1099
Size: 210 879 492 298 2254
Fragments arranged by size:
2254 879 492 298 210
BsaI GGTCTCn'nnnn_
Cuts at: 616 3837 616
Size: 3221 912
BsaAI yAC'GTr
Cuts at: 602 1486 602
Size: 884 3249
BsaBI GATnn'nnATC
Cuts at: 6 569 1647 3684 6
Size: 563 1078 2037 455
Fragments arranged by size:
2037 1078 563 455
BsaHI Gr'CG_yC
Cuts at: 167 848 954 1564 1812 2514 4010 167
Size: 681 106 610 248 702 1496 290
Fragments arranged by size:
1496 702 681 610 290 248 106
BsaWI w'CCGG_w
Cuts at: 649 1322 1843 2521 2795 2942 3353 649
Size: 673 521 678 274 147 411 1429
Fragments arranged by size:
1429 678 673 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 454 484 454
Size: 30 4103
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 264 387 949 264
Size: 123 562 3448
Fragments arranged by size:
3448 562 123
BseYI C'CCAG_C
Cuts at: 967 1033 1558 2316 2893 3389 967
Size: 66 525 758 577 496 1711
Fragments arranged by size:
1711 758 577 525 496 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 203
Size: 4133
BsiEI CG_ry'CG
Cuts at: 104 1017 1675 1721 2929 104
Size: 913 658 46 1208 1308
Fragments arranged by size:
1308 1208 913 658 46
BsiHKAI G_wGCw'C
Cuts at: 890 1147 1925 2115 2907 890
Size: 257 778 190 792 2116
Fragments arranged by size:
2116 792 778 257 190
BsmBI CGTCTCn'nnnn_
Cuts at: 158 711 158
Size: 553 3580
Bsp1286I G_dGCh'C
Cuts at: 269 301 609 890 1147 1758 1851 1925 2115
Size: 32 308 281 257 611 93 74 190
Cuts at: 2115 2177 2907 269
Size: 62 730 1495
Fragments arranged by size:
1495 730 611 308 281 257 190 93
74 62 32
BspEI T'CCGG_A
Cuts at: 3353 3353
Size: 4133
BspHI T'CATG_A
Cuts at: 407 668 890 3309 407
Size: 261 222 2419 1231
Fragments arranged by size:
2419 1231 261 222
BspMI ACCTGCnnnn'nnnn_
Cuts at: 862 1012 1183 1397 1699 2080 862
Size: 150 171 214 302 381 2915
Fragments arranged by size:
2915 381 302 214 171 150
BsrBI CCG'CTC
Cuts at: 50 950 2425 2479 3963 4128 50
Size: 900 1475 54 1484 165 55
Fragments arranged by size:
1484 1475 900 165 55 54
BsrDI GCAATG_nn'
Cuts at: 828 2045 828
Size: 1217 2916
BsrFI r'CCGG_y
Cuts at: 625 761 2131 2312 2521 625
Size: 136 1370 181 209 2237
Fragments arranged by size:
2237 1370 209 181 136
BssSI C'ACGA_G
Cuts at: 246 491 781 2404 2762 246
Size: 245 290 1623 358 1617
Fragments arranged by size:
1623 1617 358 290 245
BstAPI GCAn_nnn'nTGC
Cuts at: 1510 1510
Size: 4133
BstBI TT'CG_AA
Cuts at: 2494 2494
Size: 4133
BstEII G'GTnAC_C
Cuts at: 538 538
Size: 4133
BstYI r'GATC_y
Cuts at: 1 278 1118 1158 1625 1648 1983 2229 3241
Size: 277 840 40 467 23 335 246 1012
Cuts at: 3241 3327 3339 3685 1
Size: 86 12 346 449
Fragments arranged by size:
1012 840 467 449 346 335 277 246
86 40 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2083 2264 2427 2083
Size: 181 163 3789
Fragments arranged by size:
3789 181 163
BtsI GCAGTG_nn'
Cuts at: 256 1376 256
Size: 1120 3013
DraI TTT'AAA
Cuts at: 1132 1132
Size: 4133
DraIII CAC_nnn'GTG
Cuts at: 602 602
Size: 4133
DrdI GACnn_nn'nnGTC
Cuts at: 1048 1839 2697 1048
Size: 791 858 2484
Fragments arranged by size:
2484 858 791
EaeI y'GGCC_r
Cuts at: 272 627 777 1718 1892 2283 2310 3655 272
Size: 355 150 941 174 391 27 1345 750
Fragments arranged by size:
1345 941 750 391 355 174 150 27
EagI C'GGCC_G
Cuts at: 1718 1718
Size: 4133
EarI CTCTTCn'nnn_
Cuts at: 2156 2366 2156
Size: 210 3923
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2341 2651 2797 3943 2341
Size: 310 146 1146 2531
Fragments arranged by size:
2531 1146 310 146
EcoICRI GAG'CTC
Cuts at: 888 1145 888
Size: 257 3876
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 291 611 680 710 1958 2390 3137 3405 291
Size: 320 69 30 1248 432 747 268 1019
Fragments arranged by size:
1248 1019 747 432 320 268 69 30
EcoO109I rG'GnC_Cy
Cuts at: 1097 1272 1097
Size: 175 3958
EcoRI G'AATT_C
Cuts at: 1137 1137
Size: 4133
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2152 2152
Size: 4133
FspI TGC'GCA
Cuts at: 244 1914 244
Size: 1670 2463
FspAI rTGC'GCAy
Cuts at: 244 244
Size: 4133
HaeII r_GCGC'y
Cuts at: 957 1567 1815 2837 3816 957
Size: 610 248 1022 979 1274
Fragments arranged by size:
1274 1022 979 610 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 689 721 1975 2005 2007 2037 689
Size: 32 1254 30 2 30 2785
Fragments arranged by size:
2785 1254 32 30 30 2
HincII GTy'rAC
Cuts at: 1172 1172
Size: 4133
KasI G'GCGC_C
Cuts at: 953 1563 1811 953
Size: 610 248 3275
Fragments arranged by size:
3275 610 248
KpnI G_GTAC'C
Cuts at: 1153 1153
Size: 4133
MluI A'CGCG_T
Cuts at: 3566 3566
Size: 4133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2804 2988 2804
Size: 184 3949
MscI TGG'CCA
Cuts at: 274 779 1894 274
Size: 505 1115 2513
Fragments arranged by size:
2513 1115 505
MslI CAynn'nnrTG
Cuts at: 1084 2122 2249 1084
Size: 1038 127 2968
Fragments arranged by size:
2968 1038 127
MspA1I CmG'CkG
Cuts at: 1055 1244 1558 1918 2931 3176 1055
Size: 189 314 360 1013 245 2012
Fragments arranged by size:
2012 1013 360 314 245 189
NaeI GCC'GGC
Cuts at: 763 2314 763
Size: 1551 2582
NarI GG'CG_CC
Cuts at: 954 1564 1812 954
Size: 610 248 3275
Fragments arranged by size:
3275 610 248
NdeI CA'TA_TG
Cuts at: 420 420
Size: 4133
NgoMIV G'CCGG_C
Cuts at: 761 2312 761
Size: 1551 2582
NsiI A_TGCA'T
Cuts at: 828 828
Size: 4133
NspI r_CATG'y
Cuts at: 182 759 1186 1686 2593 182
Size: 577 427 500 907 1722
Fragments arranged by size:
1722 907 577 500 427
PciI A'CATG_T
Cuts at: 2589 2589
Size: 4133
PflMI CCAn_nnn'nTGG
Cuts at: 293 737 293
Size: 444 3689
PfoI T'CCnGG_A
Cuts at: 560 560
Size: 4133
PmeI GTTT'AAAC
Cuts at: 1132 1132
Size: 4133
PmlI CAC'GTG
Cuts at: 602 602
Size: 4133
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3272 3304 3272
Size: 32 4101
PpuMI rG'GwC_Cy
Cuts at: 1097 1097
Size: 4133
PstI C_TGCA'G
Cuts at: 859 1180 1865 859
Size: 321 685 3127
Fragments arranged by size:
3127 685 321
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4133
PvuII CAG'CTG
Cuts at: 1055 1558 1918 1055
Size: 503 360 3270
Fragments arranged by size:
3270 503 360
RsrII CG'GwC_CG
Cuts at: 2328 2328
Size: 4133
SacI G_AGCT'C
Cuts at: 890 1147 890
Size: 257 3876
SalI G'TCGA_C
Cuts at: 1170 1170
Size: 4133
SapI GCTCTTCn'nnn_
Cuts at: 2156 2366 2156
Size: 210 3923
SbfI CC_TGCA'GG
Cuts at: 859 1180 859
Size: 321 3812
ScaI AGT'ACT
Cuts at: 3650 3650
Size: 4133
SfcI C'TryA_G
Cuts at: 33 855 1176 1861 2854 3045 33
Size: 822 321 685 993 191 1121
Fragments arranged by size:
1121 993 822 685 321 191
SfoI GGC'GCC
Cuts at: 955 1565 1813 955
Size: 610 248 3275
Fragments arranged by size:
3275 610 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4133
SmaI CCC'GGG
Cuts at: 1155 1155
Size: 4133
SmlI C'TyrA_G
Cuts at: 148 1114 1288 2203 2695 2957 148
Size: 966 174 915 492 262 1324
Fragments arranged by size:
1324 966 915 492 262 174
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4133
SphI G_CATG'C
Cuts at: 1186 1686 1186
Size: 500 3633
StyI C'CwwG_G
Cuts at: 1267 1621 1267
Size: 354 3779
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1716 1849 2308 2517 1716
Size: 133 459 209 3332
Fragments arranged by size:
3332 459 209 133
TatI w'GTAC_w
Cuts at: 3648 3648
Size: 4133
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 165 197 3650 3682 165
Size: 32 3453 32 616
Fragments arranged by size:
3453 616 32 32
Tth111I GACn'n_nGTC
Cuts at: 1095 1930 1095
Size: 835 3298
XbaI T'CTAG_A
Cuts at: 1164 1164
Size: 4133
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1628 1628
Size: 4133
XmaI C'CCGG_G
Cuts at: 1153 1153
Size: 4133
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NsiI NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AleI AlfI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BmgBI BmtI Bpu10I BsiWI
BsmI BsrGI BssHII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI EcoNI EcoRV FseI HindIII HpaI MfeI NcoI
NheI NotI NruI PacI PasI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI