Restriction map for
pF1KB3443
(Circular) (Six-base) MAPSORT of: pF1KB3443.seq Check: 2788 from: 1 to: 3857
pF1KB3443 3857 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 895 895
Size: 3857
Acc65I G'GTAC_C
Cuts at: 536 873 536
Size: 337 3520
AclI AA'CG_TT
Cuts at: 851 3697 851
Size: 2846 1011
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 568 1682 2114 2861 3129 211
Size: 357 1114 432 747 268 939
Fragments arranged by size:
1114 939 747 432 357 268
AflII C'TTAA_G
Cuts at: 160 160
Size: 3857
AflIII A'CryG_T
Cuts at: 1209 2313 3290 1209
Size: 1104 977 1776
Fragments arranged by size:
1776 1104 977
AgeI A'CCGG_T
Cuts at: 2245 2245
Size: 3857
AlwNI CAG_nnn'CTG
Cuts at: 1162 2729 1162
Size: 1567 2290
ApaLI G'TGCA_C
Cuts at: 2627 2627
Size: 3857
ApoI r'AATT_y
Cuts at: 177 311 861 3210 3466 177
Size: 134 550 2349 256 568
Fragments arranged by size:
2349 568 550 256 134
AseI AT'TA_AT
Cuts at: 20 20
Size: 3857
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3857
AvaI C'yCGr_G
Cuts at: 877 877
Size: 3857
BamHI G'GATC_C
Cuts at: 882 882
Size: 3857
BanI G'GyrC_C
Cuts at: 536 873 1287 1535 1570 536
Size: 337 414 248 35 2823
Fragments arranged by size:
2823 414 337 248 35
BanII G_rGCy'C
Cuts at: 415 871 1901 415
Size: 456 1030 2371
Fragments arranged by size:
2371 1030 456
BbeI G_GCGC'C
Cuts at: 1291 1539 1291
Size: 248 3609
BbsI GAAGACnn'nnnn_
Cuts at: 740 740
Size: 3857
BbvCI CC'TCA_GC
Cuts at: 505 505
Size: 3857
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 424 458 483 517 424
Size: 34 25 34 3764
Fragments arranged by size:
3764 34 34 25
BciVI GTATCCnnnnn_n'
Cuts at: 1755 2516 3857 1755
Size: 761 1341 1755
Fragments arranged by size:
1755 1341 761
BclI T'GATC_A
Cuts at: 1377 1377
Size: 3857
BglII A'GATC_T
Cuts at: 686 1372 3409 686
Size: 686 2037 1134
Fragments arranged by size:
2037 1134 686
BlpI GC'TnA_GC
Cuts at: 969 969
Size: 3857
Bme1580I G_kGCm'C
Cuts at: 1482 1575 2631 1482
Size: 93 1056 2708
Fragments arranged by size:
2708 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1069 1150 1485 3629 1069
Size: 81 335 2144 1297
Fragments arranged by size:
2144 1297 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2224 2224
Size: 3857
Bpu10I CC'TnA_GC
Cuts at: 505 505
Size: 3857
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1033 1912 2404 2702 1033
Size: 879 492 298 2188
Fragments arranged by size:
2188 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3561 3561
Size: 3857
BsaAI yAC'GTr
Cuts at: 1210 1210
Size: 3857
BsaBI GATnn'nnATC
Cuts at: 6 1371 3408 6
Size: 1365 2037 455
Fragments arranged by size:
2037 1365 455
BsaHI Gr'CG_yC
Cuts at: 1288 1536 2238 3734 1288
Size: 248 702 1496 1411
Fragments arranged by size:
1496 1411 702 248
BsaWI w'CCGG_w
Cuts at: 1046 1567 2245 2519 2666 3077 1046
Size: 521 678 274 147 411 1826
Fragments arranged by size:
1826 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1282 2040 2617 3113 1282
Size: 758 577 496 2026
Fragments arranged by size:
2026 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1399 1445 2653 104
Size: 1295 46 1208 1308
Fragments arranged by size:
1308 1295 1208 46
BsiHKAI G_wGCw'C
Cuts at: 871 1649 1839 2631 871
Size: 778 190 792 2097
Fragments arranged by size:
2097 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 415 871 1482 1575 1649 1839 1901 2631 415
Size: 456 611 93 74 190 62 730 1641
Fragments arranged by size:
1641 730 611 456 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3077 3077
Size: 3857
BspHI T'CATG_A
Cuts at: 681 3033 681
Size: 2352 1505
BspMI ACCTGCnnnn'nnnn_
Cuts at: 366 907 1121 1423 1804 366
Size: 541 214 302 381 2419
Fragments arranged by size:
2419 541 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2149 2203 3687 3852 50
Size: 2099 54 1484 165 55
Fragments arranged by size:
2099 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1769 1769
Size: 3857
BsrFI r'CCGG_y
Cuts at: 1855 2036 2245 1855
Size: 181 209 3467
Fragments arranged by size:
3467 209 181
BsrGI T'GTAC_A
Cuts at: 299 299
Size: 3857
BssSI C'ACGA_G
Cuts at: 755 2128 2486 755
Size: 1373 358 2126
Fragments arranged by size:
2126 1373 358
BstAPI GCAn_nnn'nTGC
Cuts at: 662 1234 662
Size: 572 3285
BstBI TT'CG_AA
Cuts at: 234 315 423 2218 234
Size: 81 108 1795 1873
Fragments arranged by size:
1873 1795 108 81
BstYI r'GATC_y
Cuts at: 1 686 882 1349 1372 1707 1953 2965 3051
Size: 685 196 467 23 335 246 1012 86
Cuts at: 3051 3063 3409 1
Size: 12 346 449
Fragments arranged by size:
1012 685 467 449 346 335 246 196
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3857
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1807 1988 2151 1807
Size: 181 163 3513
Fragments arranged by size:
3513 181 163
BtsI GCAGTG_nn'
Cuts at: 1100 1100
Size: 3857
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 768 803 768
Size: 35 3822
DraI TTT'AAA
Cuts at: 856 856
Size: 3857
DraIII CAC_nnn'GTG
Cuts at: 760 760
Size: 3857
DrdI GACnn_nn'nnGTC
Cuts at: 1563 2421 1563
Size: 858 2999
EaeI y'GGCC_r
Cuts at: 1442 1616 2007 2034 3379 1442
Size: 174 391 27 1345 1920
Fragments arranged by size:
1920 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1442 1442
Size: 3857
EarI CTCTTCn'nnn_
Cuts at: 116 1880 2090 116
Size: 1764 210 1883
Fragments arranged by size:
1883 1764 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2065 2375 2521 3667 2065
Size: 310 146 1146 2255
Fragments arranged by size:
2255 1146 310 146
EcoICRI GAG'CTC
Cuts at: 869 869
Size: 3857
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 568 1682 2114 2861 3129 211
Size: 357 1114 432 747 268 939
Fragments arranged by size:
1114 939 747 432 357 268
EcoO109I rG'GnC_Cy
Cuts at: 996 996
Size: 3857
EcoRI G'AATT_C
Cuts at: 861 861
Size: 3857
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1876 1876
Size: 3857
FspI TGC'GCA
Cuts at: 1638 1638
Size: 3857
HaeII r_GCGC'y
Cuts at: 204 1291 1539 2561 3540 204
Size: 1087 248 1022 979 521
Fragments arranged by size:
1087 1022 979 521 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1699 1729 1731 1761 1699
Size: 30 2 30 3795
Fragments arranged by size:
3795 30 30 2
HincII GTy'rAC
Cuts at: 896 896
Size: 3857
HindIII A'AGCT_T
Cuts at: 250 250
Size: 3857
KasI G'GCGC_C
Cuts at: 1287 1535 1287
Size: 248 3609
KpnI G_GTAC'C
Cuts at: 540 877 540
Size: 337 3520
MluI A'CGCG_T
Cuts at: 3290 3290
Size: 3857
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 678 2528 2712 678
Size: 1850 184 1823
Fragments arranged by size:
1850 1823 184
MscI TGG'CCA
Cuts at: 1618 1618
Size: 3857
MslI CAynn'nnrTG
Cuts at: 664 1846 1973 664
Size: 1182 127 2548
Fragments arranged by size:
2548 1182 127
MspA1I CmG'CkG
Cuts at: 968 1282 1642 2655 2900 968
Size: 314 360 1013 245 1925
Fragments arranged by size:
1925 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2038 2038
Size: 3857
NarI GG'CG_CC
Cuts at: 1288 1536 1288
Size: 248 3609
NcoI C'CATG_G
Cuts at: 107 107
Size: 3857
NgoMIV G'CCGG_C
Cuts at: 2036 2036
Size: 3857
NspI r_CATG'y
Cuts at: 504 910 1410 2317 504
Size: 406 500 907 2044
Fragments arranged by size:
2044 907 500 406
PciI A'CATG_T
Cuts at: 2313 2313
Size: 3857
PflMI CCAn_nnn'nTGG
Cuts at: 760 760
Size: 3857
PmeI GTTT'AAAC
Cuts at: 856 856
Size: 3857
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2996 3028 2996
Size: 32 3825
PstI C_TGCA'G
Cuts at: 363 904 1589 363
Size: 541 685 2631
Fragments arranged by size:
2631 685 541
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3857
PvuII CAG'CTG
Cuts at: 1282 1642 1282
Size: 360 3497
RsrII CG'GwC_CG
Cuts at: 2052 2052
Size: 3857
SacI G_AGCT'C
Cuts at: 871 871
Size: 3857
SalI G'TCGA_C
Cuts at: 894 894
Size: 3857
SapI GCTCTTCn'nnn_
Cuts at: 1880 2090 1880
Size: 210 3647
SbfI CC_TGCA'GG
Cuts at: 904 904
Size: 3857
ScaI AGT'ACT
Cuts at: 3374 3374
Size: 3857
SfcI C'TryA_G
Cuts at: 33 359 900 1585 2578 2769 33
Size: 326 541 685 993 191 1121
Fragments arranged by size:
1121 993 685 541 326 191
SfoI GGC'GCC
Cuts at: 1289 1537 1289
Size: 248 3609
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3857
SmaI CCC'GGG
Cuts at: 879 879
Size: 3857
SmlI C'TyrA_G
Cuts at: 160 1012 1927 2419 2681 160
Size: 852 915 492 262 1336
Fragments arranged by size:
1336 915 852 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3857
SphI G_CATG'C
Cuts at: 910 1410 910
Size: 500 3357
StyI C'CwwG_G
Cuts at: 107 991 1345 107
Size: 884 354 2619
Fragments arranged by size:
2619 884 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 796 1440 1573 2032 2241 796
Size: 644 133 459 209 2412
Fragments arranged by size:
2412 644 459 209 133
TatI w'GTAC_w
Cuts at: 299 3372 299
Size: 3073 784
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 458 490 3374 3406 458
Size: 32 2884 32 909
Fragments arranged by size:
2884 909 32 32
Tth111I GACn'n_nGTC
Cuts at: 1654 1654
Size: 3857
XbaI T'CTAG_A
Cuts at: 888 888
Size: 3857
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 527 1352 527
Size: 825 3032
XmaI C'CCGG_G
Cuts at: 877 877
Size: 3857
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BfrBI BglI BmgBI BmtI BpmI
BsaXI BseRI BsgI BsiWI BsmI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI