Restriction map for
pF1KB3449
(Circular) (Six-base) MAPSORT of: pF1KB3449.seq Check: 4155 from: 1 to: 3710
pF1KB3449 3710 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 748 748
Size: 3710
Acc65I G'GTAC_C
Cuts at: 726 726
Size: 3710
AclI AA'CG_TT
Cuts at: 3550 3550
Size: 3710
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 98 1535 1967 2714 2982 98
Size: 1437 432 747 268 826
Fragments arranged by size:
1437 826 747 432 268
AflIII A'CryG_T
Cuts at: 1062 2166 3143 1062
Size: 1104 977 1629
Fragments arranged by size:
1629 1104 977
AgeI A'CCGG_T
Cuts at: 2098 2098
Size: 3710
AleI CACnn'nnGTG
Cuts at: 256 256
Size: 3710
AlwNI CAG_nnn'CTG
Cuts at: 1015 2582 1015
Size: 1567 2143
ApaLI G'TGCA_C
Cuts at: 2480 2480
Size: 3710
ApoI r'AATT_y
Cuts at: 301 714 3063 3319 301
Size: 413 2349 256 692
Fragments arranged by size:
2349 692 413 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3710
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3710
AvaI C'yCGr_G
Cuts at: 730 730
Size: 3710
BamHI G'GATC_C
Cuts at: 735 735
Size: 3710
BanI G'GyrC_C
Cuts at: 134 726 1140 1388 1423 134
Size: 592 414 248 35 2421
Fragments arranged by size:
2421 592 414 248 35
BanII G_rGCy'C
Cuts at: 724 1754 724
Size: 1030 2680
BbeI G_GCGC'C
Cuts at: 1144 1392 1144
Size: 248 3462
BbsI GAAGACnn'nnnn_
Cuts at: 104 104
Size: 3710
BciVI GTATCCnnnnn_n'
Cuts at: 1608 2369 3710 1608
Size: 761 1341 1608
Fragments arranged by size:
1608 1341 761
BclI T'GATC_A
Cuts at: 1230 1230
Size: 3710
BglI GCCn_nnn'nGGC
Cuts at: 632 632
Size: 3710
BglII A'GATC_T
Cuts at: 1225 3262 1225
Size: 2037 1673
BlpI GC'TnA_GC
Cuts at: 822 822
Size: 3710
Bme1580I G_kGCm'C
Cuts at: 137 265 628 1335 1428 2484 137
Size: 128 363 707 93 1056 1363
Fragments arranged by size:
1363 1056 707 363 128 93
BmrI ACTGGGnnnn_n'
Cuts at: 406 922 1003 1338 3482 406
Size: 516 81 335 2144 634
Fragments arranged by size:
2144 634 516 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2077 2077
Size: 3710
Bpu10I CC'TnA_GC
Cuts at: 446 446
Size: 3710
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 886 1765 2257 2555 886
Size: 879 492 298 2041
Fragments arranged by size:
2041 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3414 3414
Size: 3710
BsaAI yAC'GTr
Cuts at: 1063 1063
Size: 3710
BsaBI GATnn'nnATC
Cuts at: 6 1224 3261 6
Size: 1218 2037 455
Fragments arranged by size:
2037 1218 455
BsaHI Gr'CG_yC
Cuts at: 1141 1389 2091 3587 1141
Size: 248 702 1496 1264
Fragments arranged by size:
1496 1264 702 248
BsaWI w'CCGG_w
Cuts at: 899 1420 2098 2372 2519 2930 899
Size: 521 678 274 147 411 1679
Fragments arranged by size:
1679 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 627 1135 1893 2470 2966 627
Size: 508 758 577 496 1371
Fragments arranged by size:
1371 758 577 508 496
BsiEI CG_ry'CG
Cuts at: 104 1252 1298 2506 104
Size: 1148 46 1208 1308
Fragments arranged by size:
1308 1208 1148 46
BsiHKAI G_wGCw'C
Cuts at: 724 1502 1692 2484 724
Size: 778 190 792 1950
Fragments arranged by size:
1950 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 137 265 628 724 1335 1428 1502 1692 1754
Size: 128 363 96 611 93 74 190 62
Cuts at: 1754 2484 137
Size: 730 1363
Fragments arranged by size:
1363 730 611 363 190 128 96 93
74 62
BspEI T'CCGG_A
Cuts at: 2930 2930
Size: 3710
BspHI T'CATG_A
Cuts at: 2886 2886
Size: 3710
BspMI ACCTGCnnnn'nnnn_
Cuts at: 760 974 1276 1657 760
Size: 214 302 381 2813
Fragments arranged by size:
2813 381 302 214
BsrBI CCG'CTC
Cuts at: 50 560 2002 2056 3540 3705 50
Size: 510 1442 54 1484 165 55
Fragments arranged by size:
1484 1442 510 165 55 54
BsrDI GCAATG_nn'
Cuts at: 275 440 1622 275
Size: 165 1182 2363
Fragments arranged by size:
2363 1182 165
BsrFI r'CCGG_y
Cuts at: 1708 1889 2098 1708
Size: 181 209 3320
Fragments arranged by size:
3320 209 181
BssSI C'ACGA_G
Cuts at: 1981 2339 1981
Size: 358 3352
BstAPI GCAn_nnn'nTGC
Cuts at: 1087 1087
Size: 3710
BstBI TT'CG_AA
Cuts at: 2071 2071
Size: 3710
BstXI CCAn_nnnn'nTGG
Cuts at: 328 635 328
Size: 307 3403
BstYI r'GATC_y
Cuts at: 1 735 1202 1225 1560 1806 2818 2904 2916
Size: 734 467 23 335 246 1012 86 12
Cuts at: 2916 3262 1
Size: 346 449
Fragments arranged by size:
1012 734 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 266 266
Size: 3710
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1660 1841 2004 1660
Size: 181 163 3366
Fragments arranged by size:
3366 181 163
BtsI GCAGTG_nn'
Cuts at: 654 953 654
Size: 299 3411
DraI TTT'AAA
Cuts at: 186 709 186
Size: 523 3187
DrdI GACnn_nn'nnGTC
Cuts at: 1416 2274 1416
Size: 858 2852
EaeI y'GGCC_r
Cuts at: 555 1295 1469 1860 1887 3232 555
Size: 740 174 391 27 1345 1033
Fragments arranged by size:
1345 1033 740 391 174 27
EagI C'GGCC_G
Cuts at: 1295 1295
Size: 3710
EarI CTCTTCn'nnn_
Cuts at: 1733 1943 1733
Size: 210 3500
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1918 2228 2374 3520 1918
Size: 310 146 1146 2108
Fragments arranged by size:
2108 1146 310 146
EcoICRI GAG'CTC
Cuts at: 722 722
Size: 3710
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 98 1535 1967 2714 2982 98
Size: 1437 432 747 268 826
Fragments arranged by size:
1437 826 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 400 492 849 400
Size: 92 357 3261
Fragments arranged by size:
3261 357 92
EcoRI G'AATT_C
Cuts at: 714 714
Size: 3710
EcoRV GAT'ATC
Cuts at: 192 192
Size: 3710
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1729 1729
Size: 3710
FspI TGC'GCA
Cuts at: 1491 1491
Size: 3710
HaeII r_GCGC'y
Cuts at: 1144 1392 2414 3393 1144
Size: 248 1022 979 1461
Fragments arranged by size:
1461 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1552 1582 1584 1614 1552
Size: 30 2 30 3648
Fragments arranged by size:
3648 30 30 2
HincII GTy'rAC
Cuts at: 749 749
Size: 3710
KasI G'GCGC_C
Cuts at: 1140 1388 1140
Size: 248 3462
KpnI G_GTAC'C
Cuts at: 730 730
Size: 3710
MluI A'CGCG_T
Cuts at: 3143 3143
Size: 3710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2381 2565 2381
Size: 184 3526
MscI TGG'CCA
Cuts at: 1471 1471
Size: 3710
MslI CAynn'nnrTG
Cuts at: 256 1699 1826 256
Size: 1443 127 2140
Fragments arranged by size:
2140 1443 127
MspA1I CmG'CkG
Cuts at: 163 631 821 1135 1495 2508 2753 163
Size: 468 190 314 360 1013 245 1120
Fragments arranged by size:
1120 1013 468 360 314 245 190
NaeI GCC'GGC
Cuts at: 1891 1891
Size: 3710
NarI GG'CG_CC
Cuts at: 1141 1389 1141
Size: 248 3462
NgoMIV G'CCGG_C
Cuts at: 1889 1889
Size: 3710
NspI r_CATG'y
Cuts at: 763 1263 2170 763
Size: 500 907 2303
Fragments arranged by size:
2303 907 500
PciI A'CATG_T
Cuts at: 2166 2166
Size: 3710
PmeI GTTT'AAAC
Cuts at: 709 709
Size: 3710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2849 2881 2849
Size: 32 3678
PpuMI rG'GwC_Cy
Cuts at: 400 400
Size: 3710
PstI C_TGCA'G
Cuts at: 757 1442 757
Size: 685 3025
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3710
PvuII CAG'CTG
Cuts at: 163 631 1135 1495 163
Size: 468 504 360 2378
Fragments arranged by size:
2378 504 468 360
RsrII CG'GwC_CG
Cuts at: 1905 1905
Size: 3710
SacI G_AGCT'C
Cuts at: 724 724
Size: 3710
SalI G'TCGA_C
Cuts at: 747 747
Size: 3710
SanDI GG'GwC_CC
Cuts at: 400 400
Size: 3710
SapI GCTCTTCn'nnn_
Cuts at: 1733 1943 1733
Size: 210 3500
SbfI CC_TGCA'GG
Cuts at: 757 757
Size: 3710
ScaI AGT'ACT
Cuts at: 3227 3227
Size: 3710
SfcI C'TryA_G
Cuts at: 33 753 1438 2431 2622 33
Size: 720 685 993 191 1121
Fragments arranged by size:
1121 993 720 685 191
SfoI GGC'GCC
Cuts at: 1142 1390 1142
Size: 248 3462
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3710
SmaI CCC'GGG
Cuts at: 732 732
Size: 3710
SmlI C'TyrA_G
Cuts at: 865 1780 2272 2534 865
Size: 915 492 262 2041
Fragments arranged by size:
2041 915 492 262
SpeI A'CTAG_T
Cuts at: 67 582 67
Size: 515 3195
SphI G_CATG'C
Cuts at: 763 1263 763
Size: 500 3210
SspI AAT'ATT
Cuts at: 688 688
Size: 3710
StuI AGG'CCT
Cuts at: 592 592
Size: 3710
StyI C'CwwG_G
Cuts at: 844 1198 844
Size: 354 3356
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1293 1426 1885 2094 1293
Size: 133 459 209 2909
Fragments arranged by size:
2909 459 209 133
TatI w'GTAC_w
Cuts at: 3225 3225
Size: 3710
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3227 3259 3227
Size: 32 3678
Tth111I GACn'n_nGTC
Cuts at: 1507 1507
Size: 3710
XbaI T'CTAG_A
Cuts at: 741 741
Size: 3710
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1205 1205
Size: 3710
XmaI C'CCGG_G
Cuts at: 730 730
Size: 3710
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
BpmI BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI